ES2143147T3 - Metodo para la determinacion cuantitativa de creatina quinasa y reactivo apropiado para ese fin. - Google Patents
Metodo para la determinacion cuantitativa de creatina quinasa y reactivo apropiado para ese fin.Info
- Publication number
- ES2143147T3 ES2143147T3 ES96308273T ES96308273T ES2143147T3 ES 2143147 T3 ES2143147 T3 ES 2143147T3 ES 96308273 T ES96308273 T ES 96308273T ES 96308273 T ES96308273 T ES 96308273T ES 2143147 T3 ES2143147 T3 ES 2143147T3
- Authority
- ES
- Spain
- Prior art keywords
- creatine kinase
- sulfhydryl
- containing compound
- quantitatively determining
- quantitative determination
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 102000004420 Creatine Kinase Human genes 0.000 title abstract 8
- 108010042126 Creatine kinase Proteins 0.000 title abstract 8
- 238000000034 method Methods 0.000 title abstract 4
- 239000003153 chemical reaction reagent Substances 0.000 title abstract 3
- 150000001875 compounds Chemical class 0.000 abstract 4
- 125000003396 thiol group Chemical group [H]S* 0.000 abstract 4
- 229910052698 phosphorus Inorganic materials 0.000 abstract 3
- 239000011574 phosphorus Substances 0.000 abstract 3
- -1 phosphorus compound Chemical class 0.000 abstract 3
- 230000003213 activating effect Effects 0.000 abstract 1
- 239000012736 aqueous medium Substances 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
- 239000007788 liquid Substances 0.000 abstract 1
- 238000005259 measurement Methods 0.000 abstract 1
- 230000000087 stabilizing effect Effects 0.000 abstract 1
- 239000000758 substrate Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
- C12Q1/50—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving creatine phosphokinase
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
EL OBJETO DE LA INVENCION ES PROPORCIONAR UN REACTIVO PARA DETERMINAR CUANTITATIVAMENTE CINASA DE CREATINA CON CAPACIDAD DE ALMACENAMIENTO MEJORADA EN FORMA LIQUIDA ASI COMO UN METODO PARA DETERMINAR CUANTITATIVAMENTE CINASA DE CREATINA CON MEDICIONES ESTABLES. SE DISPONEN UN METODO PARA DETERMINAR CUANTITATIVAMENTE CINASA DE CREATINA, QUE COMPRENDE ACTIVAR CINASA DE CREATINA EN UNA MUESTRA EN UN MEDIO ACUOSO EN COEXISTENCIA CON UN COMPUESTO FOSFOROSO TRIVALENTE Y UN COMPUESTO QUE CONTENGA SULFHIDRILO Y A CONTINUACION DETERMINAR LA ACTIVIDAD DE CINASA DE CREATINA; UN METODO PARA ESTABILIZAR UN COMPUESTO QUE CONTENGA SULFHIDRILO, EL CUAL COMPRENDE PERMITIR QUE UN COMPUESTO FOSFOROSO TRIVALENTE COEXISTA CON UN COMPUESTO QUE CONTENGA SULFHIDRILO; Y UN REACTIVO PARA DETERMINAR CUANTITATIVAMENTE CINASA DE CREATINA, EL CUAL COMPRENDE UN COMPUESTO FOSFOROSO TRIVALENTE, UN COMPUESTO QUE CONTENGA SULFHIDRILO, Y UN SUSTRATO DE REACCION DE CINASA DE CREATINA.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP29830895 | 1995-11-16 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2143147T3 true ES2143147T3 (es) | 2000-05-01 |
Family
ID=17857970
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES96308273T Expired - Lifetime ES2143147T3 (es) | 1995-11-16 | 1996-11-15 | Metodo para la determinacion cuantitativa de creatina quinasa y reactivo apropiado para ese fin. |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US5817467A (es) |
| EP (1) | EP0774514B1 (es) |
| AT (1) | ATE190356T1 (es) |
| DE (1) | DE69606952T2 (es) |
| ES (1) | ES2143147T3 (es) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19755079A1 (de) | 1997-12-11 | 1999-06-17 | Roche Diagnostics Gmbh | Stabilisiertes Reagenz und Verfahren zur Bestimmung von Creatin-Kinase |
| EP1181356B1 (en) * | 1999-03-11 | 2005-12-07 | Eastman Chemical Company | Phosphine stabilizers for oxidoreductase enzymes |
| JP4986281B2 (ja) * | 2004-10-05 | 2012-07-25 | 旭化成ファーマ株式会社 | 補酵素の安定化方法およびその組成物 |
| TW200808974A (en) * | 2006-08-02 | 2008-02-16 | Ind Tech Res Inst | Luminescence-based recipe |
| CN112881311B (zh) * | 2019-11-29 | 2025-05-30 | 深圳迈瑞生物医疗电子股份有限公司 | 肌酸激酶检测试剂、试剂盒和检测方法 |
| CN113866410A (zh) * | 2021-11-04 | 2021-12-31 | 广州达安基因股份有限公司 | 一种测定肌酸激酶同工酶活性的试剂盒及其测定方法 |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2908054A1 (de) * | 1979-03-02 | 1980-09-11 | Boehringer Mannheim Gmbh | Verfahren und reagenz zur bestimmung der creatinkinase |
| JPS5934119A (ja) * | 1982-08-20 | 1984-02-24 | Yamatake Honeywell Co Ltd | 流量積算表示回路を有する湯沸器 |
| US4547461A (en) * | 1983-01-18 | 1985-10-15 | Eastman Kodak Company | Composition, analytical element and method for the quantification of creatine kinase |
| JPS6188899A (ja) * | 1984-10-05 | 1986-05-07 | Unitika Ltd | クレアチンキナ−ゼ定量用試薬 |
| US4701420A (en) * | 1985-04-01 | 1987-10-20 | Eastman Kodak Company | Analytical compositions, elements and methods utilizing reduction of ferric ion chelates to form detectable dyes |
| JPS62104598A (ja) * | 1985-11-01 | 1987-05-15 | Yatoron:Kk | クレアチンキナ−ゼ測定用試薬 |
| US5250420A (en) * | 1987-04-02 | 1993-10-05 | Toyo Boseki Kabushiki Kaisha | Method and reagent for determination of dehydrogenase or its substrate |
| JPH07102156B2 (ja) * | 1987-04-02 | 1995-11-08 | 東洋紡績株式会社 | 脱水素酵素または基質の測定法 |
| JPS6428799A (en) * | 1987-07-24 | 1989-01-31 | Tokyo Sokushin Kk | Switching device for earthquake control circuit |
| JPH07114711B2 (ja) | 1987-11-11 | 1995-12-13 | 協和メデックス株式会社 | 還元型補酵素の定量法 |
| CA2028593C (en) * | 1989-10-31 | 2007-08-21 | Douglas R. Brandt | Stabilized enzyme compositions |
| DE4029585A1 (de) * | 1990-09-14 | 1992-03-19 | Pharma Hameln Gmbh | Verfahren zur stabilisierung sterilisierter pharmazeutischer loesungen mit oxidationsempfindlichen bestandteilen sowie zusammensetzung zur verwendung als stabilisator fuer derartige loesungen |
| JPH0724957B2 (ja) * | 1991-07-31 | 1995-03-22 | 株式会社神戸製鋼所 | 固体超高圧プレスの圧力制御方法 |
| JP3203108B2 (ja) * | 1993-08-26 | 2001-08-27 | 協和メデックス株式会社 | グルコース−6−リン酸デヒドロゲナーゼの安定化方法 |
-
1996
- 1996-11-14 US US08/748,803 patent/US5817467A/en not_active Expired - Fee Related
- 1996-11-15 ES ES96308273T patent/ES2143147T3/es not_active Expired - Lifetime
- 1996-11-15 AT AT96308273T patent/ATE190356T1/de not_active IP Right Cessation
- 1996-11-15 DE DE69606952T patent/DE69606952T2/de not_active Expired - Fee Related
- 1996-11-15 EP EP96308273A patent/EP0774514B1/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| DE69606952D1 (de) | 2000-04-13 |
| EP0774514A1 (en) | 1997-05-21 |
| HK1001311A1 (en) | 1998-06-12 |
| ATE190356T1 (de) | 2000-03-15 |
| EP0774514B1 (en) | 2000-03-08 |
| US5817467A (en) | 1998-10-06 |
| DE69606952T2 (de) | 2000-07-20 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG2A | Definitive protection |
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