ES2145838T3 - Procedimiento para la obtencion recombinante de proteinas en la levadura hansenula. - Google Patents

Procedimiento para la obtencion recombinante de proteinas en la levadura hansenula.

Info

Publication number
ES2145838T3
ES2145838T3 ES94927528T ES94927528T ES2145838T3 ES 2145838 T3 ES2145838 T3 ES 2145838T3 ES 94927528 T ES94927528 T ES 94927528T ES 94927528 T ES94927528 T ES 94927528T ES 2145838 T3 ES2145838 T3 ES 2145838T3
Authority
ES
Spain
Prior art keywords
pct
proteins
procedure
date mar
hansenula
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES94927528T
Other languages
English (en)
Inventor
Juergen Schweden
Claus Bollschweiler
Michael Piontek
Ulrike Weydemann
Zbigniew A Janowicz
Alexander W M Strasser
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BASF SE
Dynavax GmbH
Original Assignee
BASF SE
Rhein Biotech GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by BASF SE, Rhein Biotech GmbH filed Critical BASF SE
Application granted granted Critical
Publication of ES2145838T3 publication Critical patent/ES2145838T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

PROCEDIMIENTO PARA LA SINTESIS RECOMBINANTE DE PROTEINAS EN LA LEVADURA HANSENULA, CARACTERIZADO, PORQUE HANSENULA SE TRANSFORMA CON UNA EXPRESION QUE TIENE LOS SIGUIENTES ELEMENTOS ESTRUCTURALES: L-A-P-GEN, DONDE L: UN DIRECTOR DE SECUENCIA, A: ES UN ADAPTADOR QUE GENERA UNA ESTRUCTURA EN ALFA-HELICE, P: UNA SEÑAL DE PROCESAMIENTO, Y GEN: UN GEN ESTRUCTURAL PARA LA PROTEINA DESEADA.
ES94927528T 1993-09-04 1994-09-01 Procedimiento para la obtencion recombinante de proteinas en la levadura hansenula. Expired - Lifetime ES2145838T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE4329969A DE4329969A1 (de) 1993-09-04 1993-09-04 Verfahren zur rekombinanten Herstellung von Proteinen in Hefe

Publications (1)

Publication Number Publication Date
ES2145838T3 true ES2145838T3 (es) 2000-07-16

Family

ID=6496878

Family Applications (1)

Application Number Title Priority Date Filing Date
ES94927528T Expired - Lifetime ES2145838T3 (es) 1993-09-04 1994-09-01 Procedimiento para la obtencion recombinante de proteinas en la levadura hansenula.

Country Status (11)

Country Link
US (1) US5741674A (es)
EP (1) EP0716705B1 (es)
JP (1) JPH09502347A (es)
AT (1) ATE191007T1 (es)
CA (1) CA2169567A1 (es)
DE (2) DE4329969A1 (es)
DK (1) DK0716705T3 (es)
ES (1) ES2145838T3 (es)
GR (1) GR3033114T3 (es)
PT (1) PT716705E (es)
WO (1) WO1995007356A1 (es)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19620443C2 (de) * 1996-05-21 1998-09-17 Rhein Biotech Proz & Prod Gmbh Verfahren zum automatischen Durchführen von Bluttests
EP1373476B1 (en) 2001-03-09 2011-06-15 University Of Kentucky Research Foundation Cytochrome p450s and uses thereof
HUE050997T2 (hu) * 2008-05-06 2021-03-01 Archer Daniels Midland Co Keményítõ- és xilánbontó enzimek expresszálásával keményítõ és xilán alkoholos fermentációjára képes hõtûrõ Hansenula polymorpha élesztõtörzsek kifejlesztése
US9452212B2 (en) 2011-04-14 2016-09-27 Dynavax Technologies Corporation Methods and compositions for eliciting an immune response against hepatitis B virus

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0423890A3 (en) * 1984-07-27 1991-07-03 Unilever Nv Use of oxidoreductases in bleaching and/or detergent compositions and their preparation by microorganisms engineered by recombinant dna technology
WO1992013951A1 (en) * 1991-02-04 1992-08-20 The Salk Institute Biotechnology/Industrial Associates, Inc. Production of human serum albumin in methylotrophic yeast cells

Also Published As

Publication number Publication date
PT716705E (pt) 2000-09-29
DK0716705T3 (da) 2000-07-10
WO1995007356A1 (de) 1995-03-16
EP0716705B1 (de) 2000-03-22
JPH09502347A (ja) 1997-03-11
ATE191007T1 (de) 2000-04-15
EP0716705A1 (de) 1996-06-19
GR3033114T3 (en) 2000-08-31
DE4329969A1 (de) 1995-03-09
US5741674A (en) 1998-04-21
DE59409242D1 (de) 2000-04-27
CA2169567A1 (en) 1995-03-16

Similar Documents

Publication Publication Date Title
AU1575088A (en) Process for the purification of recombinant polypeptides
NZ232742A (en) Yeast heterologous protein production method, vectors, dna constructs and proteins so produced and aprotinin (1-58) so produced
ES2068907T3 (es) Secuencias de nucleotidos que codifican una proteina con actividad ureasica.
GB2197324B (en) Process for the production of a polypeptide
PT850310E (pt) Molecula de dna circular com origem de replicacao condicional, metodo para a sua preparacao e utilizacao da mesma na terapia genica
EP0330451A3 (en) A cdna coding for human normal serum albumin a, and a process for production of the albumin
EP0823438A3 (en) Human mucin cone protein: peptide fragments and antibodies thereto, and uses thereof in diagnostic and therapeutic methods
EP0276723A3 (de) Vorläuferprotein des APC-Polypeptids, dafür codierende DNA und diagnostische Verwendung der DNA und des Proteins
IE883612L (en) Vectors and compounds for direct expression of activated human protein c
AU5053796A (en) Cytoplasmic expression of antibodies, antibody fragments and antibody fragment fusion molecules in E. Coli
CA2260365A1 (en) Differentiation-suppressive polypeptide
ES2145838T3 (es) Procedimiento para la obtencion recombinante de proteinas en la levadura hansenula.
HU9401604D0 (en) Production of proteins using fb2 protein
AU7873798A (en) N-terminally extended proteins expressed in yeast
DE3877035D1 (de) Gentechnologisches verfahren zur herstellung von polypeptiden.
AU630287B2 (en) Process for producing foreign proteins in streptomyces
ES2146666T3 (es) Procedimiento para la obtencion recombinante de proteinas en levadura.
PT93614A (pt) Estirpe de levedura melhorada para a producao de proteinas heterologas maduras,em particular hirudina, e processo para a preparacao da hirudina correspondente
AU6134596A (en) Enamel matrix related polypeptide
EE03074B1 (et) Rekombinantsed eukarüootsed rakud, nende saamiseks vajalikud DNA järjestused, meetod eukarüootsete rakkude konstrueerimiseks ja sekreteeritavate valkude ning biomassi tootmismeetod
FI870501A0 (fi) Menetelmä melanoomaan liittyvän p97-antigeenin antigeenisen sukulaispeptidin tai -proteiinin valmistamiseksi, sekä siinä käyttökelpoiset yhdistelmävirukset, yhdistelmävektorit ja isäntäsolut
EP0497911A4 (en) Recombinant production of lactoperoxidase
GR890100542A (el) Συστηματα εκφρασεως για την παρασκευη πολυπεπτιδιων σε προκαρυοτικα κυτταρα.

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 716705

Country of ref document: ES