ES2147121A1 - Nuevos biocatalizadores de lipasas inmovilizadas. - Google Patents

Nuevos biocatalizadores de lipasas inmovilizadas.

Info

Publication number
ES2147121A1
ES2147121A1 ES009800707A ES9800707A ES2147121A1 ES 2147121 A1 ES2147121 A1 ES 2147121A1 ES 009800707 A ES009800707 A ES 009800707A ES 9800707 A ES9800707 A ES 9800707A ES 2147121 A1 ES2147121 A1 ES 2147121A1
Authority
ES
Spain
Prior art keywords
lipases
derivatives
new
supports
adsorption
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
ES009800707A
Other languages
English (en)
Other versions
ES2147121B1 (es
Inventor
Seijas Jose Manuel Guisan
Gil Pilar Armisen
Castrillo Pilar Sabuquillo
Lorente Gloria Fernandez
Lafuente Roberto Fernandez
Codina Agatha Bastida
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Vita Invest SA
Original Assignee
Vita Invest SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Vita Invest SA filed Critical Vita Invest SA
Priority to ES9800707A priority Critical patent/ES2147121B1/es
Priority to AU29353/99A priority patent/AU2935399A/en
Priority to PCT/ES1999/000075 priority patent/WO1999051726A1/es
Publication of ES2147121A1 publication Critical patent/ES2147121A1/es
Application granted granted Critical
Publication of ES2147121B1 publication Critical patent/ES2147121B1/es
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

Nuevos biocatalizadores de lipasas inmovilizadas. Nuevos biocatalizadores de lipasas inmovilizadas por adsorción sobre soportes hidrofílicos recubiertos químicamente de una densa capa de grupos altamente hidrofóbicos. La preparación de estos nuevos derivados inmovilizados de lipasas comprende: la activación de soportes hidrofílicos con una adecuada morfología interna con grupos hidrofóbicos de estructura definida; y la adsorción, a muy baja fuerza iónica, de extractos de diferentes lipasas comerciales sobre estos soportes fabricados a medida. Los derivados obtenidos muestran una excelente actividad y estabilidad en ausencia de nuevas interfases, en algunos casos una actividad más de 100 veces más alta que los correspondientes enzimas solubles. Estos derivados de lipasas purificadas e hiperactivadas serán muy útiles como catalizadores industriales de hidrólisis enantio y regioselectiva de intermedios farmacéuticos especialmente cuando estos procesos se realicen en medios acuosos.
ES9800707A 1998-04-03 1998-04-03 Nuevos biocatalizadores de lipasas inmovilizadas. Expired - Fee Related ES2147121B1 (es)

Priority Applications (3)

Application Number Priority Date Filing Date Title
ES9800707A ES2147121B1 (es) 1998-04-03 1998-04-03 Nuevos biocatalizadores de lipasas inmovilizadas.
AU29353/99A AU2935399A (en) 1998-04-03 1999-03-24 Immobilized lipase biocatalysts
PCT/ES1999/000075 WO1999051726A1 (es) 1998-04-03 1999-03-24 Biocatalizadores de lipasas inmovilizadas

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES9800707A ES2147121B1 (es) 1998-04-03 1998-04-03 Nuevos biocatalizadores de lipasas inmovilizadas.

Publications (2)

Publication Number Publication Date
ES2147121A1 true ES2147121A1 (es) 2000-08-16
ES2147121B1 ES2147121B1 (es) 2001-04-16

Family

ID=8303330

Family Applications (1)

Application Number Title Priority Date Filing Date
ES9800707A Expired - Fee Related ES2147121B1 (es) 1998-04-03 1998-04-03 Nuevos biocatalizadores de lipasas inmovilizadas.

Country Status (3)

Country Link
AU (1) AU2935399A (es)
ES (1) ES2147121B1 (es)
WO (1) WO1999051726A1 (es)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2161167A1 (es) * 1999-09-24 2001-11-16 Vita Invest Sa Procedimiento de hidrolisis enzimatica de esteres del acido (3sr,4rs)-4-(4-flurofenil)-6-oxopiperidin-3-carboxilico con biocatalizadores de lipasas o esterasas inmovilizadas.

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2203319B1 (es) * 2002-04-03 2005-03-01 Universidad De Oviedo Nuevos carbonatos opticamente activos como intermedios en la sintesis de (+)-zopiclona.

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0322213A2 (en) * 1987-12-22 1989-06-28 Unilever Plc Process for the preparation of fatty acid esters
WO1990015868A1 (en) * 1989-06-21 1990-12-27 Novo Nordisk A/S Immobilized lipase preparation and use thereof for ester synthesis
EP0579928A1 (en) * 1992-05-25 1994-01-26 The Nisshin Oil Mills, Ltd. Immobilized lipase, process for producing the same and process for transesterifying oil and fat with the same
WO1994028118A1 (en) * 1993-05-20 1994-12-08 Loders Croklaan B.V. Immobilized lipases

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0322213A2 (en) * 1987-12-22 1989-06-28 Unilever Plc Process for the preparation of fatty acid esters
WO1990015868A1 (en) * 1989-06-21 1990-12-27 Novo Nordisk A/S Immobilized lipase preparation and use thereof for ester synthesis
EP0579928A1 (en) * 1992-05-25 1994-01-26 The Nisshin Oil Mills, Ltd. Immobilized lipase, process for producing the same and process for transesterifying oil and fat with the same
WO1994028118A1 (en) * 1993-05-20 1994-12-08 Loders Croklaan B.V. Immobilized lipases

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
YAMANÉ, T. et al. "Repeated use of lipase immobilized on amphiphilic gel fo hydrolysis of a small amount of glycerides included in liquid crude fatty acid", J. Fermentation Technol., 1982, Vol. 60 (6), paginas 517-523 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2161167A1 (es) * 1999-09-24 2001-11-16 Vita Invest Sa Procedimiento de hidrolisis enzimatica de esteres del acido (3sr,4rs)-4-(4-flurofenil)-6-oxopiperidin-3-carboxilico con biocatalizadores de lipasas o esterasas inmovilizadas.

Also Published As

Publication number Publication date
AU2935399A (en) 1999-10-25
ES2147121B1 (es) 2001-04-16
WO1999051726A1 (es) 1999-10-14

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