ES2147149B1 - PROCEDURE FOR THE DETECTION OF THE GROWTH OF MICROORGANISMS IN BIOLOGICAL FLUIDS AND CLINICAL SAMPLES THROUGH ULTRASONICS. - Google Patents

PROCEDURE FOR THE DETECTION OF THE GROWTH OF MICROORGANISMS IN BIOLOGICAL FLUIDS AND CLINICAL SAMPLES THROUGH ULTRASONICS.

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Publication number
ES2147149B1
ES2147149B1 ES9802442A ES9802442A ES2147149B1 ES 2147149 B1 ES2147149 B1 ES 2147149B1 ES 9802442 A ES9802442 A ES 9802442A ES 9802442 A ES9802442 A ES 9802442A ES 2147149 B1 ES2147149 B1 ES 2147149B1
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ES
Spain
Prior art keywords
pulse
procedure
microorganisms
growth
detection
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
ES9802442A
Other languages
Spanish (es)
Other versions
ES2147149A1 (en
Inventor
De Espinosa Freijo Fra Montero
Vera Juan Ramon Maestre
Rodriguez Gloria Tardajos
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Consejo Superior de Investigaciones Cientificas CSIC
Original Assignee
Consejo Superior de Investigaciones Cientificas CSIC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Consejo Superior de Investigaciones Cientificas CSIC filed Critical Consejo Superior de Investigaciones Cientificas CSIC
Priority to ES9802442A priority Critical patent/ES2147149B1/en
Publication of ES2147149A1 publication Critical patent/ES2147149A1/en
Application granted granted Critical
Publication of ES2147149B1 publication Critical patent/ES2147149B1/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Procedimiento para la detección del crecimiento de microorganismos en fluidos biológicos y muestras clínicas mediante ultrasonidos. El procedimiento consiste en la medida automática de la velocidad de propagación de una señal impulsiva con una frecuencia central de 5 MHz a través de un recipiente estéril que contiene un medio de cultivo líquido al que previamente se le ha inoculado una muestra clínica de sangre, orina u otro fluido orgánico. El recipiente y la muestra líquida han de permanecer a una temperatura controlada y fija. En condiciones estándar se fija en 35ºC. Las técnica de medida puede utilizar bien la configuración de transmisión-through-transmission- con dos transductores piezoeléctricos enfrentados, bien la de pulso-eco - pulse-echo- con un sólo transductor y un reflector enfrentado y paralelo a la superficie de emisión del mismo. El pulso ultrasónico es procesado después de atravesar el recipiente, calculando por procedimientos digitales la diferencia en tiempo entre la señal de disparo y la llegada de dicho pulso. **FORMULA**Procedure for the detection of the growth of microorganisms in biological fluids and clinical samples by ultrasound. The procedure consists in the automatic measurement of the propagation speed of an impulse signal with a central frequency of 5 MHz through a sterile container that contains a liquid culture medium that has previously been inoculated with a clinical sample of blood, urine or other organic fluid. The container and the liquid sample must remain at a controlled and fixed temperature. Under standard conditions it is set at 35 ° C. The measurement techniques can use either the transmission-through-transmission configuration with two piezoelectric transducers facing each other, or the pulse-echo-pulse-echo- with a single transducer and a reflector facing and parallel to the emission surface thereof . The ultrasonic pulse is processed after crossing the vessel, calculating by digital procedures the difference in time between the trigger signal and the arrival of said pulse. **FORMULA**

ES9802442A 1998-11-20 1998-11-20 PROCEDURE FOR THE DETECTION OF THE GROWTH OF MICROORGANISMS IN BIOLOGICAL FLUIDS AND CLINICAL SAMPLES THROUGH ULTRASONICS. Expired - Fee Related ES2147149B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES9802442A ES2147149B1 (en) 1998-11-20 1998-11-20 PROCEDURE FOR THE DETECTION OF THE GROWTH OF MICROORGANISMS IN BIOLOGICAL FLUIDS AND CLINICAL SAMPLES THROUGH ULTRASONICS.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES9802442A ES2147149B1 (en) 1998-11-20 1998-11-20 PROCEDURE FOR THE DETECTION OF THE GROWTH OF MICROORGANISMS IN BIOLOGICAL FLUIDS AND CLINICAL SAMPLES THROUGH ULTRASONICS.

Publications (2)

Publication Number Publication Date
ES2147149A1 ES2147149A1 (en) 2000-08-16
ES2147149B1 true ES2147149B1 (en) 2001-03-01

Family

ID=8305866

Family Applications (1)

Application Number Title Priority Date Filing Date
ES9802442A Expired - Fee Related ES2147149B1 (en) 1998-11-20 1998-11-20 PROCEDURE FOR THE DETECTION OF THE GROWTH OF MICROORGANISMS IN BIOLOGICAL FLUIDS AND CLINICAL SAMPLES THROUGH ULTRASONICS.

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ES (1) ES2147149B1 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2197022B1 (en) * 2002-06-14 2005-03-01 Consejo Sup Investi. Cientificas NON-INVASIVE PROCEDURE FOR DETECTION AND ANALYSIS OF MICROORGANISMS IN DAIRY PRODUCTS IN LAMINATED PAPER OR CARTON PACKS USING ELASTIC WAVES BY DRY COUPLING.
ATE342376T1 (en) * 2002-06-14 2006-11-15 Consejo Superior Investigacion NON-INVASIVE ANALYSIS AND DETECTION OF MICROORGANISMS IN DAIRY PRODUCTS LOCATED IN LAMINATED PAPER OR CARDBOARD CONTAINERS USING ELASTIC WAVES BY DRY COUPLING
US12560578B2 (en) 2023-09-19 2026-02-24 Fresenius Medical Care Holdings, Inc. Ultrasonic detection of bacteria in water of a medical system

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DD229430A1 (en) * 1984-12-06 1985-11-06 Inst Tech Mikrobiologie METHOD FOR CONTROLLING THE GROWTH OF MICROORGANISMS IN BIOLOGICAL SYSTEMS
DD286873A5 (en) * 1989-08-10 1991-02-07 Tech Hochschule C Schorlemmer METHOD AND DEVICE FOR CARRYING OUT ULTRASOUND DAMAGE MEASUREMENTS ON MICROBIOLOGICAL SUSPENSIONS
JPH06261793A (en) * 1993-03-16 1994-09-20 Kawasaki Heavy Ind Ltd Method and apparatus for measuring microalgae growth amount
JPH08285837A (en) * 1995-04-17 1996-11-01 Kurita Water Ind Ltd Sludge activity measuring device

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Publication number Publication date
ES2147149A1 (en) 2000-08-16

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