ES2153745A1 - Dispositivo compartimentado y metodo para la captura y doble amplificacion enzimatica de secuencias diana de acidos nucleicos en un solo dispositivo compartimentado cerrado. - Google Patents
Dispositivo compartimentado y metodo para la captura y doble amplificacion enzimatica de secuencias diana de acidos nucleicos en un solo dispositivo compartimentado cerrado.Info
- Publication number
- ES2153745A1 ES2153745A1 ES009801642A ES9801642A ES2153745A1 ES 2153745 A1 ES2153745 A1 ES 2153745A1 ES 009801642 A ES009801642 A ES 009801642A ES 9801642 A ES9801642 A ES 9801642A ES 2153745 A1 ES2153745 A1 ES 2153745A1
- Authority
- ES
- Spain
- Prior art keywords
- nucleic acid
- reverse transcription
- amplification
- enzymatic amplification
- acid sequences
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 230000003321 amplification Effects 0.000 title abstract 6
- 230000002255 enzymatic effect Effects 0.000 title abstract 6
- 238000003199 nucleic acid amplification method Methods 0.000 title abstract 6
- 238000006243 chemical reaction Methods 0.000 title abstract 5
- 150000007523 nucleic acids Chemical group 0.000 title abstract 3
- 238000010839 reverse transcription Methods 0.000 title abstract 3
- 238000000034 method Methods 0.000 abstract 3
- 239000003153 chemical reaction reagent Substances 0.000 abstract 2
- 238000001514 detection method Methods 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Dispositivo compartimentado y método de captura y doble amplificación enzimática de secuencias diana de ácidos nucleicos en un solo dispositivo compartimentado cerrado. El dispositivo compartimentado (1) consta de un tubo de reacción que comprende dos compartimentos (2) y (3), que configuran dos zonas físicamente separadas y destinadas a contener reactivos, y una tapa (4). El método incluye el empleo de dicho dispositivo y comprende la captura, específica o no, de una secuencia diana, su transcripción reversa (en su caso), una primera amplificación enzimática y una segunda amplificación enzimática de una diana presente en los productos resultantes de la primera amplificación enzimática, en un solo dispositivo que contiene todos los reactivos necesarios para dichas reacciones y sin necesidad de abrir el dispositivo una vez iniciada la reacción de transcripción reversa (en su caso) o la primera reacción amplificación enzimática. El método tiene aplicación en la detección de dianas de ácidos nucleicos.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES9801642A ES2153745B1 (es) | 1998-07-31 | 1998-07-31 | Dispositivo compartimentado y metodo para la captura y doble amplificacion enzimatica de secuencias diana de acidos nucleicos en un solo dispositivo compartimentado cerrado. |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES9801642A ES2153745B1 (es) | 1998-07-31 | 1998-07-31 | Dispositivo compartimentado y metodo para la captura y doble amplificacion enzimatica de secuencias diana de acidos nucleicos en un solo dispositivo compartimentado cerrado. |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| ES2153745A1 true ES2153745A1 (es) | 2001-03-01 |
| ES2153745B1 ES2153745B1 (es) | 2001-11-16 |
Family
ID=8304741
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES9801642A Expired - Fee Related ES2153745B1 (es) | 1998-07-31 | 1998-07-31 | Dispositivo compartimentado y metodo para la captura y doble amplificacion enzimatica de secuencias diana de acidos nucleicos en un solo dispositivo compartimentado cerrado. |
Country Status (1)
| Country | Link |
|---|---|
| ES (1) | ES2153745B1 (es) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2183695A1 (es) * | 2000-10-31 | 2003-03-16 | Ivia | Metodo y mezcla de reaccion para la amplificacion enzimatica simultanea, coordinada y cooperativa de secuencias diana de acidos nucleicos. |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1991012342A1 (en) * | 1990-02-16 | 1991-08-22 | F. Hoffmann-La Roche Ag | Improvements in the specificity and convenience of the polymerase chain reaction |
| WO1991018110A1 (en) * | 1990-05-16 | 1991-11-28 | Mats Malmquist | Method and means to perform biochemical reactions |
| WO1992020778A1 (en) * | 1991-05-24 | 1992-11-26 | Kindconi Pty Limited | Biochemical reaction control |
| WO1994026414A1 (en) * | 1993-05-17 | 1994-11-24 | Syntex (U.S.A.) Inc. | Reaction container for specific binding assays and method for its use |
| US5576197A (en) * | 1995-04-07 | 1996-11-19 | Molecular Bio-Products | Polymerase chain reaction container and methods of using the same |
-
1998
- 1998-07-31 ES ES9801642A patent/ES2153745B1/es not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1991012342A1 (en) * | 1990-02-16 | 1991-08-22 | F. Hoffmann-La Roche Ag | Improvements in the specificity and convenience of the polymerase chain reaction |
| WO1991018110A1 (en) * | 1990-05-16 | 1991-11-28 | Mats Malmquist | Method and means to perform biochemical reactions |
| WO1992020778A1 (en) * | 1991-05-24 | 1992-11-26 | Kindconi Pty Limited | Biochemical reaction control |
| WO1994026414A1 (en) * | 1993-05-17 | 1994-11-24 | Syntex (U.S.A.) Inc. | Reaction container for specific binding assays and method for its use |
| US5576197A (en) * | 1995-04-07 | 1996-11-19 | Molecular Bio-Products | Polymerase chain reaction container and methods of using the same |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2183695A1 (es) * | 2000-10-31 | 2003-03-16 | Ivia | Metodo y mezcla de reaccion para la amplificacion enzimatica simultanea, coordinada y cooperativa de secuencias diana de acidos nucleicos. |
Also Published As
| Publication number | Publication date |
|---|---|
| ES2153745B1 (es) | 2001-11-16 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EC2A | Search report published |
Date of ref document: 20010301 Kind code of ref document: A1 Effective date: 20010301 |
|
| FD2A | Announcement of lapse in spain |
Effective date: 20110819 |