ES2154648T3 - Deteccion y amplificacion de acidos nucleicos blanco con actividad exonucleolitica. - Google Patents

Deteccion y amplificacion de acidos nucleicos blanco con actividad exonucleolitica.

Info

Publication number
ES2154648T3
ES2154648T3 ES93917324T ES93917324T ES2154648T3 ES 2154648 T3 ES2154648 T3 ES 2154648T3 ES 93917324 T ES93917324 T ES 93917324T ES 93917324 T ES93917324 T ES 93917324T ES 2154648 T3 ES2154648 T3 ES 2154648T3
Authority
ES
Spain
Prior art keywords
amplification
exonucleolitic
activity
detection
nucleic acids
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES93917324T
Other languages
English (en)
Inventor
John J Carrinno
Uwe Spies
Laurie A Rinehardt
Edward K Pabich
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Abbott Laboratories
Original Assignee
Abbott Laboratories
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Abbott Laboratories filed Critical Abbott Laboratories
Application granted granted Critical
Publication of ES2154648T3 publication Critical patent/ES2154648T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6862—Ligase chain reaction [LCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

LA INVENCION SE REFIERE A ESQUEMAS MEJORADOS DE AMPLIFICACION LCR QUE UTILIZAN AL MENOS UNA SONDA MODIFICADA EN SU EXTREMO 5'' PARA REDUCIR O ELIMINAR LA AMPLIFICACION INDEPENDIENTE DEL OBJETIVO. TAMBIEN SE PRESENTAN LAS SONDAS DIFERENTES, MODIFICADAS Y LOS EQUIPOS QUE LAS CONTIENEN. TAMBIEN SE PRESENTA UN METODO PARA DETECTAR LAS DIFERENCIAS EN SECUENCIAS DE ACIDO NUCLEICO, CON UNA AMPLIFICACION REDUCIDA INDEPENDIENTE DEL OBJETIVO, UTILIZANDO LAS ONDAS MODIFICADAS.
ES93917324T 1992-08-03 1993-07-21 Deteccion y amplificacion de acidos nucleicos blanco con actividad exonucleolitica. Expired - Lifetime ES2154648T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US92540292A 1992-08-03 1992-08-03

Publications (1)

Publication Number Publication Date
ES2154648T3 true ES2154648T3 (es) 2001-04-16

Family

ID=25451687

Family Applications (1)

Application Number Title Priority Date Filing Date
ES93917324T Expired - Lifetime ES2154648T3 (es) 1992-08-03 1993-07-21 Deteccion y amplificacion de acidos nucleicos blanco con actividad exonucleolitica.

Country Status (7)

Country Link
EP (1) EP0654093B1 (es)
JP (1) JP3516953B2 (es)
AU (1) AU4687393A (es)
CA (1) CA2140331C (es)
DE (1) DE69329824T2 (es)
ES (1) ES2154648T3 (es)
WO (1) WO1994003636A1 (es)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5593840A (en) * 1993-01-27 1997-01-14 Oncor, Inc. Amplification of nucleic acid sequences
US5888731A (en) * 1995-08-30 1999-03-30 Visible Genetics Inc. Method for identification of mutations using ligation of multiple oligonucleotide probes
US6852487B1 (en) 1996-02-09 2005-02-08 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
EP1736554B1 (en) 1996-05-29 2013-10-09 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
GB9902000D0 (en) 1999-01-30 1999-03-17 Delta Biotechnology Ltd Process
JP2004507230A (ja) * 2000-07-03 2004-03-11 アプレラ　コーポレイション ポリヌクレオチド配列アッセイ
EP1327682B1 (de) * 2002-01-11 2009-05-13 BioSpring Gesellschaft für Biotechnologie mbH Verfahren zur Herstellung von DNA
CN120138110B (zh) * 2025-03-14 2026-04-07 湖南大地同年生物科技有限公司 多重pcr检测反应中代替高温变性产生单链的方法和提高多重pcr产物液相杂交检测灵敏性和准确性的方法及配套的试剂盒

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0379559B1 (en) * 1988-06-24 1996-10-23 Amgen Inc. Method and reagents for detecting nucleic acid sequences
DE69118930T2 (de) * 1990-01-26 1997-01-09 Abbott Lab Verbessertes Verfahren zur Amplifikation von Nuklein säurezielsequenz, einsetzbar für die Polymerase und Ligasekettenreaktion
US5427930A (en) * 1990-01-26 1995-06-27 Abbott Laboratories Amplification of target nucleic acids using gap filling ligase chain reaction
DE69133629D1 (de) * 1990-05-03 2010-04-15 Cornell Res Foundation Inc nssystem zur Bestimmung von genetischen Krankheiten
US5210015A (en) * 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
CA2136764A1 (en) * 1992-05-29 1993-12-09 Ronald L. Marshall Ligase chain reaction starting with rna sequences

Also Published As

Publication number Publication date
DE69329824T2 (de) 2001-08-09
CA2140331C (en) 2000-01-18
AU4687393A (en) 1994-03-03
WO1994003636A1 (en) 1994-02-17
DE69329824D1 (de) 2001-02-08
EP0654093A4 (en) 1998-01-07
JPH08501212A (ja) 1996-02-13
EP0654093B1 (en) 2001-01-03
JP3516953B2 (ja) 2004-04-05
EP0654093A1 (en) 1995-05-24
CA2140331A1 (en) 1994-02-17

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