ES2155342A1 - Mejoras de cepas de streptomyces mediante la utilizacion de genes biosinteticos de tilosina. - Google Patents

Mejoras de cepas de streptomyces mediante la utilizacion de genes biosinteticos de tilosina.

Info

Publication number
ES2155342A1
ES2155342A1 ES009801682A ES9801682A ES2155342A1 ES 2155342 A1 ES2155342 A1 ES 2155342A1 ES 009801682 A ES009801682 A ES 009801682A ES 9801682 A ES9801682 A ES 9801682A ES 2155342 A1 ES2155342 A1 ES 2155342A1
Authority
ES
Spain
Prior art keywords
tylosine
genes
streptomyces
fradiae
strains
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
ES009801682A
Other languages
English (en)
Other versions
ES2155342B1 (es
Inventor
Martinez Roberto Fouces
Duran Encarnacion Mellado
Garcia Bruno Diez
Fuente Jose Luis Barredo
Morales Manuel Esteban
Ermanno Bernasconi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Antibioticos SA
Original Assignee
Antibioticos SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Antibioticos SA filed Critical Antibioticos SA
Priority to ES009801682A priority Critical patent/ES2155342B1/es
Priority to AU52911/99A priority patent/AU5291199A/en
Priority to PCT/ES1999/000248 priority patent/WO2000008168A1/es
Publication of ES2155342A1 publication Critical patent/ES2155342A1/es
Application granted granted Critical
Publication of ES2155342B1 publication Critical patent/ES2155342B1/es
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/36—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Actinomyces; from Streptomyces (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0077—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with a reduced iron-sulfur protein as one donor (1.14.15)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1007—Methyltransferases (general) (2.1.1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1077—Pentosyltransferases (2.4.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/60—Preparation of O-glycosides, e.g. glucosides having an oxygen of the saccharide radical directly bound to a non-saccharide heterocyclic ring or a condensed ring system containing a non-saccharide heterocyclic ring, e.g. coumermycin, novobiocin
    • C12P19/62—Preparation of O-glycosides, e.g. glucosides having an oxygen of the saccharide radical directly bound to a non-saccharide heterocyclic ring or a condensed ring system containing a non-saccharide heterocyclic ring, e.g. coumermycin, novobiocin the hetero ring having eight or more ring members and only oxygen as ring hetero atoms, e.g. erythromycin, spiramycin, nystatin

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Mejoras de cepas de Streptomyces mediante la utilización de genes biosintéticos de tilosina. La presente invención se refiere a un procedimiento basado en la utilización de genes del cluster biosintético de tilosina para el incremento de la producción de tilosina en S. fradiae o para la producción de nuevos metabolitos híbridos en microorganismos relacionados (Streptomyces spp.). En él se describe: (I) un método para aislar un fragmento de ADN de S. fradiae que contiene 11 genes, 10 de ellos pertenecientes al cluster de genes biosintéticos de tilosina y (11) la expresión de dichos genes en el microorganismo producto de tilosina S. fradiae, así como en otros microorganismos relacios (Streptomyces spp.) consiguiendo un incremento en la producción de tilosina en el primer caso y la producción de nuevos antibióticos híbridos en el segundo.
ES009801682A 1998-08-05 1998-08-05 Mejoras de cepas de streptomyces mediante la utilizacion de genes biosinteticos de tilosina. Expired - Fee Related ES2155342B1 (es)

Priority Applications (3)

Application Number Priority Date Filing Date Title
ES009801682A ES2155342B1 (es) 1998-08-05 1998-08-05 Mejoras de cepas de streptomyces mediante la utilizacion de genes biosinteticos de tilosina.
AU52911/99A AU5291199A (en) 1998-08-05 1999-08-02 Improvements to strains of streptomyces through the use of biosynthetic genes oftylosine
PCT/ES1999/000248 WO2000008168A1 (es) 1998-08-05 1999-08-02 Mejora de cepas de streptomyces mediante la utilizacion de genes biosinteticos de tilosina

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES009801682A ES2155342B1 (es) 1998-08-05 1998-08-05 Mejoras de cepas de streptomyces mediante la utilizacion de genes biosinteticos de tilosina.

Publications (2)

Publication Number Publication Date
ES2155342A1 true ES2155342A1 (es) 2001-05-01
ES2155342B1 ES2155342B1 (es) 2001-12-01

Family

ID=8304796

Family Applications (1)

Application Number Title Priority Date Filing Date
ES009801682A Expired - Fee Related ES2155342B1 (es) 1998-08-05 1998-08-05 Mejoras de cepas de streptomyces mediante la utilizacion de genes biosinteticos de tilosina.

Country Status (3)

Country Link
AU (1) AU5291199A (es)
ES (1) ES2155342B1 (es)
WO (1) WO2000008168A1 (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR122014018568B1 (pt) * 2001-10-19 2015-12-01 Sumitomo Chemical Co métodos para conferir a uma planta resistência a um herbicida, controle de ervas daninhas, bem como avaliar a resistência de uma célula ou planta a um composto herbicida
WO2015138494A1 (en) * 2014-03-10 2015-09-17 Georges Belfort Anti-microbial peptides and method for designing novel anti-microbial peptides

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0364130A2 (en) * 1988-09-29 1990-04-18 Eli Lilly And Company Novel tylosin biosynthetic genes
US5672497A (en) * 1986-03-21 1997-09-30 Eli Lilly And Company Method for increasing the antibiotic-producing ability of antibiotic-producing microorganisms

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5672497A (en) * 1986-03-21 1997-09-30 Eli Lilly And Company Method for increasing the antibiotic-producing ability of antibiotic-producing microorganisms
EP0364130A2 (en) * 1988-09-29 1990-04-18 Eli Lilly And Company Novel tylosin biosynthetic genes

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
FISHMAN, S.E. et al. "Cloning genes for the biosynthesis of a macrolide antibiotic", PROC. NATL. ACAD. SCI. USA, 1987, Vol. 84, paginas 8248-8252. Todo el documento. *
GANDECHA, A.R. et al. "Analysis of four tylosin biosynthesis genes from the tylLM region of the Streptomyces fradial genome", GENE, 1997, Vol. 184, paginas 197-203. Todo el documento. *

Also Published As

Publication number Publication date
AU5291199A (en) 2000-02-28
ES2155342B1 (es) 2001-12-01
WO2000008168A1 (es) 2000-02-17

Similar Documents

Publication Publication Date Title
AU5102899A (en) Process and materials for production of glucosamine
EP0913466A3 (en) Method for producing L-leucine
CA2289968A1 (en) A method of producing butanol using a mutant strain of clostridium beijerinckii
ZA963737B (en) Bioconversion of a fermentable carbon source to 1,3-propanediol by a single microorganism.
BR9811248B1 (pt) variante de enzima subtilase derivada de uma subtilase originária selecionada a partir do sub-grupo i-s1 ou do sub-grupo i-s2, dita variante tendo melhorado desempenho de lavagem em detergentes em comparação com a subtilase originária, sequência de dna isolada, vetor de expressão, célula hospedeira microbiana, processo para produzir uma variante, composição, uso de uma variante de subtilase.
EP1118667A4 (en) NOVEL ENZYME COMPOSITIONS, PROCESS FOR PRODUCING SUCH COMPOSITIONS, AND USE THEREOF
DE3571646D1 (en) Process for the amplification of the expression of a determined gene in bacillus subtilis and strains obtained
ES2155342A1 (es) Mejoras de cepas de streptomyces mediante la utilizacion de genes biosinteticos de tilosina.
WO2000037669A3 (en) Non-continuous process for the production of vitamin b12
AU7298600A (en) Method for producing exopolysaccharides
IL110430A (en) Dna sequence comprising a gene cluster encoding branched chain alpha-ketoacid dehydrogenase complex from streptomyces avermitilis
EP1170370A3 (en) Method for producing nucleotide by fermentation
EP1225217A4 (en) PROCESS FOR PRODUCING TRANSGLUTAMINASE DERIVED FROM A MICROORGANISM
WO2004106557A3 (en) Thiamin production by fermentation
AU698813B2 (en) Process for producing transglutaminase by microorganism
BG104759A (en) Streptomyces avermitilis gene directing the ratio of b2:b1 avermectins
YU8402A (sh) Gen streptomyces avermitilis koji usmerava odnos b2:b1 avermektina
BG103704A (en) New compounds
GB9828711D0 (en) Production of proteins in gram-positive microorganisms
AU2001258400A1 (en) Method for introducing a nucleic acid into a cell (transfection) by means of calcium phosphate, and transfected cell
NZ511728A (en) Substances WK-5344A and WK-5344B obtained from microorganisms of the genus Streptomyces
EP1063288A3 (en) Method for producing L-lysine
AU2781297A (en) Biosynthesis method enabling the preparation of cobalamins
AP2002002418A0 (en) Streptomyces avermitilis gene directing the ratio of B2:B1 avermectins.
GB9424950D0 (en) Novel process

Legal Events

Date Code Title Description
EC2A Search report published

Date of ref document: 20010501

Kind code of ref document: A1

Effective date: 20010501

FD1A Patent lapsed

Effective date: 20070806