ES2158900T3 - Biosensor potenciometrico y metodo de utilizacion del mismo. - Google Patents

Biosensor potenciometrico y metodo de utilizacion del mismo.

Info

Publication number
ES2158900T3
ES2158900T3 ES94922667T ES94922667T ES2158900T3 ES 2158900 T3 ES2158900 T3 ES 2158900T3 ES 94922667 T ES94922667 T ES 94922667T ES 94922667 T ES94922667 T ES 94922667T ES 2158900 T3 ES2158900 T3 ES 2158900T3
Authority
ES
Spain
Prior art keywords
reagent
analyte
enzyme
potentiometric
biosensor
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES94922667T
Other languages
English (en)
Inventor
Kent M Kost
Thomas J Lindsay
John F Price
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics Corp
Original Assignee
Roche Diagnostics Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Roche Diagnostics Corp filed Critical Roche Diagnostics Corp
Application granted granted Critical
Publication of ES2158900T3 publication Critical patent/ES2158900T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • C12Q1/004—Enzyme electrodes mediator-assisted
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/817—Enzyme or microbe electrode

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

UN BIOSENSOR POTENCIOMETRICO, TIRA DE PRUEBA, REACTIVO Y UN METODO POTENCIOMETRICO PARA DETECCION O MEDICION DE UNA ANALITA DE UNA MUESTRA LIQUIDA. EL BIOSENSOR INCLUYE ELECTRODOS DE INDICACION (3) Y REFERENCIA, (5), Y UN REACTIVO (9), QUE SE EXTIENDE SOBRE UNA PARTE DE LA SUPERFICIE DEL ELECTRODO DE INDICACION (3). EL REACTIVO INCLUYE GENERALMENTE UNA ENZIMA Y UN MEDIADOR DE REDOX. CUANDO UNA MUESTRA DE FLUIDO (POR EJEMPLO, SANGRE) QUE CONTIENE UNA ANALITA (POR EJEMPLO, GLUCOSA) SE AÑADE AL REACTIVO, LA ENZIMA (POR EJEMPLO, OXIDASA DE GLUCOSA) CATALIZA UNA ENZIMA QUE IMPLICA UNA REACCION, UN MEDIADOR DE REDOX (POR EJEMPLO, FERRICIANURO POTASICO), Y UNA ANALITA. ESTA REACCION PUEDE SER MONITORIZADA MEDIANTE LA MONITORIZACION DEL POTENCIAL ELECTRICO EN LA SUPERFICIE DEL ELECTRODO DE INDICACION. EL BIOSENSOR POTENCIOMETRICO INVENTADO MIDE EL CAMBIO DE POTENCIAL EN LA SUPERFICIE DEL ELECTRODO DE INDICACION DESPUES DE QUE LA MUESTRA DE FLUIDO QUE CONTIENE LA ANALITA ES AÑADIDA AL REACTIVO. EL CAMBIO EN EL POTENCIAL QUE SE PRODUCE PUEDE ESTAR RELACIONADO CON LA DETECCION O MEDICION DE ANALITA EN LA MUESTRA DE FLUIDO.
ES94922667T 1993-07-23 1994-07-21 Biosensor potenciometrico y metodo de utilizacion del mismo. Expired - Lifetime ES2158900T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/097,331 US5413690A (en) 1993-07-23 1993-07-23 Potentiometric biosensor and the method of its use

Publications (1)

Publication Number Publication Date
ES2158900T3 true ES2158900T3 (es) 2001-09-16

Family

ID=22262812

Family Applications (1)

Application Number Title Priority Date Filing Date
ES94922667T Expired - Lifetime ES2158900T3 (es) 1993-07-23 1994-07-21 Biosensor potenciometrico y metodo de utilizacion del mismo.

Country Status (7)

Country Link
US (1) US5413690A (es)
EP (1) EP0710358B1 (es)
JP (1) JP3387926B2 (es)
CA (1) CA2167822C (es)
DE (1) DE69427353T2 (es)
ES (1) ES2158900T3 (es)
WO (1) WO1995003543A1 (es)

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