ES2159372T3 - Procedimiento de preparacion de aspartasa y de acido l-aspartico. - Google Patents

Procedimiento de preparacion de aspartasa y de acido l-aspartico.

Info

Publication number
ES2159372T3
ES2159372T3 ES97104568T ES97104568T ES2159372T3 ES 2159372 T3 ES2159372 T3 ES 2159372T3 ES 97104568 T ES97104568 T ES 97104568T ES 97104568 T ES97104568 T ES 97104568T ES 2159372 T3 ES2159372 T3 ES 2159372T3
Authority
ES
Spain
Prior art keywords
aspartase
producing
culture
acid
aspartico
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES97104568T
Other languages
English (en)
Inventor
Junichi Takano
Kuniki Kino
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
KH Neochem Co Ltd
Original Assignee
Kyowa Hakko Kogyo Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kyowa Hakko Kogyo Co Ltd filed Critical Kyowa Hakko Kogyo Co Ltd
Application granted granted Critical
Publication of ES2159372T3 publication Critical patent/ES2159372T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/20—Aspartic acid; Asparagine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/02—Atmosphere, e.g. low oxygen conditions

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

SE DESCRIBE UN PROCEDIMIENTO PARA PRODUCIR ASPARTASA QUE CONSISTE EN CULTIVAR UN MICROORGANISMO CAPAZ DE PRODUCIR ASPARTASA PERTENECIENTE AL GENERO ESCHERICHIA EN UN MEDIO, HASTA QUE SE PRODUCE Y ACUMULA ASPARTASA EN EL CULTIVO, Y RECUPERAR LA ASPARTASA DEL MEDIO, ESTANDO LA CONCENTRACION DE OXIGENO DISUELTO EN EL MEDIO EN EL RANGO DE 0 A 1 PPM EN LA FASE DE CRECIMIENTO MEDIO DEL MICROORGANISMO Y/O EN LA ULTIMA FASE DE CRECIMIENTO LOGARITMICO; Y UN PROCEDIMIENTO PARA PRODUCIR ACIDO L - ASPARTICO QUE CONSISTE EN CONVERTIR ACIDO FUMARICO Y AMONIACO EN ACIDO L ASPARTICO EN MEDIO ACUOSO Y EN PRESENCIA DE UNA FUENTE ENZIMATICA, LA CUAL ES UN CULTIVO PRODUCIDO SEGUN EL PROCEDIMIENTO MENCIONADO DE PRODUCCION DE ASPARTASA, CELULAS AISLADAS DE UN CULTIVO O LAS MISMAS CELULAS PROCESADAS.
ES97104568T 1996-03-29 1997-03-18 Procedimiento de preparacion de aspartasa y de acido l-aspartico. Expired - Lifetime ES2159372T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP7598996 1996-03-29

Publications (1)

Publication Number Publication Date
ES2159372T3 true ES2159372T3 (es) 2001-10-01

Family

ID=13592199

Family Applications (1)

Application Number Title Priority Date Filing Date
ES97104568T Expired - Lifetime ES2159372T3 (es) 1996-03-29 1997-03-18 Procedimiento de preparacion de aspartasa y de acido l-aspartico.

Country Status (12)

Country Link
US (1) US5916782A (es)
EP (1) EP0798377B1 (es)
KR (1) KR970065723A (es)
CN (1) CN1165860A (es)
AT (1) ATE201443T1 (es)
AU (1) AU708588B2 (es)
CA (1) CA2200420A1 (es)
DE (1) DE69704900T2 (es)
DK (1) DK0798377T3 (es)
ES (1) ES2159372T3 (es)
PT (1) PT798377E (es)
TW (1) TW487733B (es)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5939296A (en) * 1996-08-09 1999-08-17 Nippon Shokubai Co., Ltd. Process for producing L-aspartic acid
EP0945513B1 (en) * 1998-02-13 2005-09-21 Nippon Shokubai Co., Ltd. Method for producing L-aspartic acid
AT408755B (de) * 1998-05-22 2002-03-25 Dsm Fine Chem Austria Gmbh Verfahren zur herstellung von l-asparaginsäure
EP2080769A3 (en) 2004-07-02 2010-12-01 Metanomics GmbH Process for the production of fine chemicals
CA2585798A1 (en) * 2004-12-17 2006-06-17 Metanomics Gmbh Process for the control of production of fine chemicals
CN102559538B (zh) * 2011-11-25 2014-04-09 烟台恒源生物股份有限公司 高产l-天冬氨酸酶的大肠埃希氏菌及其应用
CN102660527B (zh) * 2012-05-30 2013-07-03 山东大学 利用原料预处理和补料发酵生产高酶活碱性果胶酶的方法
CN104593306B (zh) * 2015-02-04 2017-06-23 烟台恒源生物股份有限公司 一种大肠埃希氏菌株hy‑05c高密度培养方法
CN104845921B (zh) * 2015-06-15 2018-02-27 中国食品发酵工业研究院 一株噬菌体抗性l‑天冬氨酸转化菌及其应用
CN105316273B (zh) * 2015-11-24 2019-08-30 南京工业大学 一株无苹果酸副产的l-天冬氨酸酶重组大肠杆菌及其构建方法与应用
CN108531525A (zh) * 2018-05-09 2018-09-14 宿州学院 一种l-天冬氨酸的酶法转化制备方法
CN109337941A (zh) * 2018-09-29 2019-02-15 宿州学院 一种l-天冬氨酸的质控生产方法及其应用
CN109970587B (zh) * 2019-05-06 2024-04-30 宿州学院 一种l-天冬氨酸分离提纯装置及其分离提纯l-天冬氨酸的方法

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5412553A (en) * 1977-06-29 1979-01-30 Toshiba Corp Microwave oscillation circuit
JPS5718867A (en) * 1980-07-08 1982-01-30 Nissan Motor Co Ltd Pressure control valve
ZA824805B (en) * 1981-10-15 1983-04-27 Genex Corp Production of aspartase
US4436813A (en) * 1982-03-16 1984-03-13 Purification Engineering, Inc. Immobilized microbial cell composition for making L-aspartic acid
EP0102207A3 (en) * 1982-08-13 1986-03-26 The Board Of Trustees Of The Leland Stanford Junior University Method of immobilizing enzymes
JPS59113895A (ja) * 1982-12-03 1984-06-30 Tanabe Seiyaku Co Ltd Ｌ−アスパラギン酸の製法
US4560653A (en) * 1983-06-06 1985-12-24 W. R. Grace & Co. Process for preparing L-aspartic acid
JPS59232088A (ja) * 1983-06-15 1984-12-26 Tanabe Seiyaku Co Ltd Ｌ−アスパラギン酸の製法
JPS60133883A (ja) * 1983-12-20 1985-07-17 Tanabe Seiyaku Co Ltd 新規微生物及びそれを用いるｌ−アスパラギン酸の製法
US4698224A (en) * 1984-04-10 1987-10-06 Kirin Beer Kabushiki Kaisha Production of alcoholic beverages
JP2501124B2 (ja) * 1989-07-25 1996-05-29 松下電工株式会社 プリント基板の小径穴あけ方法
JP3009257B2 (ja) * 1991-07-25 2000-02-14 三菱化学株式会社 アスパルターゼをコードする遺伝子ｄｎａ及びその利用
US5441634A (en) * 1993-07-06 1995-08-15 Edwards Laboratories, Inc. Apparatus and method of circulating a body of fluid containing a mixture of solid waste and water and separating them
JP2664648B2 (ja) * 1994-05-20 1997-10-15 株式会社日本触媒 Ｌ−アスパラギン酸の製造方法
US5677156A (en) * 1995-04-04 1997-10-14 Mitsubishi Chemical Corporation Method for producing fumaric acid
US5939296A (en) * 1996-08-09 1999-08-17 Nippon Shokubai Co., Ltd. Process for producing L-aspartic acid

Also Published As

Publication number Publication date
CN1165860A (zh) 1997-11-26
US5916782A (en) 1999-06-29
PT798377E (pt) 2001-08-30
EP0798377B1 (en) 2001-05-23
KR970065723A (ko) 1997-10-13
EP0798377A3 (en) 1998-09-09
AU1650697A (en) 1997-10-02
DE69704900D1 (de) 2001-06-28
TW487733B (en) 2002-05-21
DK0798377T3 (da) 2001-07-30
CA2200420A1 (en) 1997-09-29
AU708588B2 (en) 1999-08-05
EP0798377A2 (en) 1997-10-01
ATE201443T1 (de) 2001-06-15
DE69704900T2 (de) 2001-10-25

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