ES2164536B1 - Procedimiento para el aislamiento y crecimiento de bacterias capaces de utilizar el manitol como fuente de carbono. - Google Patents
Procedimiento para el aislamiento y crecimiento de bacterias capaces de utilizar el manitol como fuente de carbono.Info
- Publication number
- ES2164536B1 ES2164536B1 ES9901455A ES9901455A ES2164536B1 ES 2164536 B1 ES2164536 B1 ES 2164536B1 ES 9901455 A ES9901455 A ES 9901455A ES 9901455 A ES9901455 A ES 9901455A ES 2164536 B1 ES2164536 B1 ES 2164536B1
- Authority
- ES
- Spain
- Prior art keywords
- medium
- procedure
- carbon source
- growth
- bacteria
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 241000894006 Bacteria Species 0.000 title abstract 3
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 title abstract 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 title abstract 2
- 229910052799 carbon Inorganic materials 0.000 title abstract 2
- 238000000034 method Methods 0.000 title abstract 2
- 238000009413 insulation Methods 0.000 title 1
- 239000002609 medium Substances 0.000 abstract 7
- 229930195725 Mannitol Natural products 0.000 abstract 2
- 239000001963 growth medium Substances 0.000 abstract 2
- 238000002955 isolation Methods 0.000 abstract 2
- 239000000594 mannitol Substances 0.000 abstract 2
- 235000010355 mannitol Nutrition 0.000 abstract 2
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Chemical class [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 abstract 2
- 239000000725 suspension Substances 0.000 abstract 2
- 241001633102 Rhizobiaceae Species 0.000 abstract 1
- 229940041514 candida albicans extract Drugs 0.000 abstract 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical class [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 abstract 1
- 229910000396 dipotassium phosphate Inorganic materials 0.000 abstract 1
- 235000019797 dipotassium phosphate Nutrition 0.000 abstract 1
- 230000007613 environmental effect Effects 0.000 abstract 1
- 229910052500 inorganic mineral Inorganic materials 0.000 abstract 1
- 239000000463 material Substances 0.000 abstract 1
- 239000011707 mineral Chemical class 0.000 abstract 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 abstract 1
- 235000019796 monopotassium phosphate Nutrition 0.000 abstract 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical class [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 abstract 1
- 150000003839 salts Chemical class 0.000 abstract 1
- 239000012137 tryptone Chemical class 0.000 abstract 1
- 229940088594 vitamin Drugs 0.000 abstract 1
- 229930003231 vitamin Natural products 0.000 abstract 1
- 235000013343 vitamin Nutrition 0.000 abstract 1
- 239000011782 vitamin Substances 0.000 abstract 1
- 239000012138 yeast extract Chemical class 0.000 abstract 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Procedimiento y medio de cultivo para el aislamiento y crecimiento de bacterias incluidas en la familia rhizobiaceae: rhizobios y agrobacteria y cualquier bacteria capaz de utilizar el manitol como fuente de carbono, en muestras de procedencia humana, animal, vegetal, industrial o medioambiental y, en general, de cualquier tipo de muestras, que comprende las etapas de: A: Si el medio se va a utilizar como medio de aislamiento:(I) Preparar una suspensión densa del material. (II) incubar el medio a la temperatura óptima de crecimiento. B: Si el medio va a utilizarse como medio de cultivo: (I) sembrar una colonia o suspensión de colonias procedentes de un cultivo puro en el medio. (II) incubar el medio a la temperatura óptima de crecimiento caracterizado porque el medio proporcionado se compone de: manitol, fosfato monopotásico, fosfato dipotásico, extracto de levadura, triptona, sales minerales, vitaminas.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES9901455A ES2164536B1 (es) | 1999-06-30 | 1999-06-30 | Procedimiento para el aislamiento y crecimiento de bacterias capaces de utilizar el manitol como fuente de carbono. |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES9901455A ES2164536B1 (es) | 1999-06-30 | 1999-06-30 | Procedimiento para el aislamiento y crecimiento de bacterias capaces de utilizar el manitol como fuente de carbono. |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| ES2164536A1 ES2164536A1 (es) | 2002-02-16 |
| ES2164536B1 true ES2164536B1 (es) | 2003-04-01 |
Family
ID=8309039
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES9901455A Expired - Fee Related ES2164536B1 (es) | 1999-06-30 | 1999-06-30 | Procedimiento para el aislamiento y crecimiento de bacterias capaces de utilizar el manitol como fuente de carbono. |
Country Status (1)
| Country | Link |
|---|---|
| ES (1) | ES2164536B1 (es) |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5576874A (en) * | 1978-10-27 | 1980-06-10 | Kitasato Inst:The | Novel antibiotic a40a and its preparation |
| ES2099679B1 (es) * | 1995-11-03 | 1998-01-16 | Univ Granada | Utilizacion de rhizobium leguminosarum bv. viceae cepa z25 como inoculante para el cultivo de plantas leguminosas. |
| TW491892B (en) * | 1996-11-07 | 2002-06-21 | Srl Inc | Apparatus for detecting microorganism |
-
1999
- 1999-06-30 ES ES9901455A patent/ES2164536B1/es not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| ES2164536A1 (es) | 2002-02-16 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EC2A | Search report published |
Date of ref document: 20020216 Kind code of ref document: A1 Effective date: 20020216 |
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| FG2A | Definitive protection |
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| FD2A | Announcement of lapse in spain |
Effective date: 20191025 |