ES2168848T3 - Metodo de seleccion por polarizacion de fluorescencia. - Google Patents

Metodo de seleccion por polarizacion de fluorescencia.

Info

Publication number
ES2168848T3
ES2168848T3 ES99906092T ES99906092T ES2168848T3 ES 2168848 T3 ES2168848 T3 ES 2168848T3 ES 99906092 T ES99906092 T ES 99906092T ES 99906092 T ES99906092 T ES 99906092T ES 2168848 T3 ES2168848 T3 ES 2168848T3
Authority
ES
Spain
Prior art keywords
fluorescence polarization
selection
transferase
determination
compound
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES99906092T
Other languages
English (en)
Inventor
Lars Kongsbak
Kristian Skovgaard Joergensen
Jesper Valbjoern
Christel Thea Joergensen
Tommy Lykke Husum
Steffen Ernst
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novozymes AS
Original Assignee
Novozymes AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes AS filed Critical Novozymes AS
Application granted granted Critical
Publication of ES2168848T3 publication Critical patent/ES2168848T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/40—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving amylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6445—Measuring fluorescence polarisation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/91—Transferases (2.)
    • G01N2333/91045—Acyltransferases (2.3)
    • G01N2333/91074—Aminoacyltransferases (general) (2.3.2)
    • G01N2333/9108—Aminoacyltransferases (general) (2.3.2) with definite EC number (2.3.2.-)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/916—Hydrolases (3) acting on ester bonds (3.1), e.g. phosphatases (3.1.3), phospholipases C or phospholipases D (3.1.4)
    • G01N2333/918—Carboxylic ester hydrolases (3.1.1)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/924—Hydrolases (3) acting on glycosyl compounds (3.2)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Materials By The Use Of Optical Means Adapted For Particular Applications (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Método para seleccionar una secuencia nucleótida que codifica un compuesto biológico que comprende: a) expresar la secuencia nucleótida en un sistema de expresión para producir el compuesto biológico, b) poner en contacto el compuesto biológico con una sustancia fluorescente capaz de reaccionar ante el compuesto biológico para dejar que la reacción tenga lugar, c) medir la polarización de fluorescencia de la sustancia fluorescente, y d) seleccionar los sistemas de expresión en los que haya ocurrido un cambio en la polarización de fluorescencia.
ES99906092T 1998-03-06 1999-03-05 Metodo de seleccion por polarizacion de fluorescencia. Expired - Lifetime ES2168848T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DK30898 1998-03-06

Publications (1)

Publication Number Publication Date
ES2168848T3 true ES2168848T3 (es) 2002-06-16

Family

ID=8092089

Family Applications (1)

Application Number Title Priority Date Filing Date
ES99906092T Expired - Lifetime ES2168848T3 (es) 1998-03-06 1999-03-05 Metodo de seleccion por polarizacion de fluorescencia.

Country Status (11)

Country Link
EP (2) EP1156122A3 (es)
JP (1) JP2002505114A (es)
CN (2) CN1560609A (es)
AT (1) ATE208828T1 (es)
AU (1) AU2612299A (es)
CA (1) CA2322488A1 (es)
DE (1) DE69900465T2 (es)
DK (1) DK1058738T3 (es)
ES (1) ES2168848T3 (es)
PT (1) PT1058738E (es)
WO (1) WO1999045143A2 (es)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2328719A1 (en) * 1998-04-15 1999-10-21 Hiroyoshi Hidaka Method for in vivo detecting target protein gene by drug
DE60016951T2 (de) * 1999-10-01 2005-12-15 Novozymes A/S Zellulosefilme zur durchfuehrung von screening-verfahren
US6426189B1 (en) 1999-10-01 2002-07-30 Novozymes A/S Cellulose films for screening
AU7646100A (en) * 1999-11-05 2001-05-14 Novozymes A/S A high throughput screening (hts) method
US6723537B2 (en) 2001-05-18 2004-04-20 Rigel Pharmaceuticals, Incorporated Directed evolution of protein in mammalian cells
JP4607950B2 (ja) * 2004-03-18 2011-01-05 トランス テック ファーマ，インコーポレイテッド 蛍光偏光アッセイ
FR2897164B1 (fr) 2006-02-09 2008-03-14 Commissariat Energie Atomique Realisation de cavites pouvant etre remplies par un materiau fluidique dans un compose microtechnologique optique
FR2929715B1 (fr) 2008-04-04 2010-09-10 Commissariat Energie Atomique Procede de realisation de cavites microniques ou submicroniques
FR2933205B1 (fr) 2008-06-27 2010-08-13 Commissariat Energie Atomique Procede ameliore de realisation d'enceintes remplies de liquide et fermees par une membrane
FR2938834B1 (fr) * 2008-11-27 2011-03-04 Commissariat Energie Atomique Procede de realisation d'une matrice de retention et comprenant un liquide fonctionnel
FR2963112B1 (fr) 2010-07-21 2013-02-15 Commissariat Energie Atomique Microstructure a parois a propriete optique determinee et procede de realisation de microstructures
WO2014067933A1 (en) 2012-10-31 2014-05-08 C-Lecta Gmbh Bioactive carrier preparation for enhanced safety in care products and food
KR101494881B1 (ko) 2013-05-06 2015-02-24 한국과학기술연구원 설퍼 옥시도리덕타아제 결합 형광 화합물을 이용한 바실러스 서브틸리스 검출 방법
CN105866316B (zh) * 2016-06-24 2018-03-30 曲阜师范大学 一种同时检测食品中氨基酸和生物胺的分析方法
CN110954517A (zh) * 2019-12-11 2020-04-03 西北大学 还原性糖的一步荧光衍生方法及其应用
CN113514445B (zh) * 2021-08-05 2023-12-26 荆州市第一人民医院（荆州市肿瘤医院、长江大学附属第一医院） 一种基于荧光偏振的去泛素化酶活性检测方法
CN114166807B (zh) * 2021-11-22 2023-12-12 中国农业大学 用于检测多粘菌素的荧光偏振免疫分析方法
WO2025168975A1 (en) * 2024-02-10 2025-08-14 Biuro Techniczno-Prawne Adam Turczyński Method of manufacturing a probe for determining the activity of pectinolytic enzymes and method of determining the activity of pectinolytic enzymes using said probe
WO2025168976A1 (en) * 2024-02-10 2025-08-14 Biuro Techniczno-Prawne Adam Turczyński Method for detecting pectinolytic pathogens of crops and ornamental plants, microbial medium for use in this method and test kit implementing this method

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE78517T1 (de) * 1985-02-25 1992-08-15 Abbott Lab Polarisierter fluoreszenztest von makromolekularen hydrolasen.
MX9206979A (es) * 1991-12-04 1993-07-01 Novo Nordisk As Metodo de clonacion de proteinas en levaduras
JPH0723800A (ja) * 1993-07-08 1995-01-27 Tanabe Seiyaku Co Ltd 核酸の検出方法
GB9519393D0 (en) * 1995-09-22 1995-11-22 Univ Edinburgh Novel compounds and assay method

Also Published As

Publication number Publication date
CN1297487A (zh) 2001-05-30
EP1156122A3 (en) 2005-02-09
CA2322488A1 (en) 1999-09-10
CN1560609A (zh) 2005-01-05
EP1058738B1 (en) 2001-11-14
CN1167810C (zh) 2004-09-22
DE69900465T2 (de) 2002-09-05
DE69900465D1 (de) 2001-12-20
JP2002505114A (ja) 2002-02-19
WO1999045143A2 (en) 1999-09-10
PT1058738E (pt) 2002-05-31
AU2612299A (en) 1999-09-20
ATE208828T1 (de) 2001-11-15
EP1156122A2 (en) 2001-11-21
EP1058738A2 (en) 2000-12-13
DK1058738T3 (da) 2002-03-11
WO1999045143A3 (en) 1999-10-21

Similar Documents

Publication Publication Date Title
ES2168848T3 (es) Metodo de seleccion por polarizacion de fluorescencia.
ES2182234T3 (es) Inhibidores de metaloproteasa.
BR9506535A (pt) Inibidores de metaloproteinases
BG101833A (bg) Гуаниново производно
ES2193709T3 (es) Nuevos efectores de la dipeptidil-peptidasa iv.
ATE296833T1 (de) Nukleinsäure-markierungsverbindungen
AU7316900A (en) Compounds for fluorescence labeling
PT788605E (pt) Deteccao de anticorpos bacterianos por polarizacao de fluorescencia
TR200000586T2 (tr) Farmasötik maddelerin oral yoldan biyolojik kullanışlılığının arttırılması için bir yöntem, bileşimler ve kitler.
ES2136887T3 (es) Derivados antifungicos de sordaridina.
FI954029A0 (fi) Kemiluminesoiva reagenssi ja analyysimenetelmä, jossa käytetään substituoitua asetanilidia valon muodostukseen
BR9607164A (pt) Processo dosagem ultra-sensível da troponina i cardíaca
ATE250021T1 (de) Hydroxycyclopentanone
FI913611A7 (fi) Menetelmä elohopean toteamiseksi elohopeasta aiheutuneen kohonneen bio luminesenssin omaavien mikro-organismien avulla
ATE205822T1 (de) Substituierte 4-arylbutyrsäurederivate als matrix metalloprotease inhibitoren
FI882057A7 (fi) Menetelmä polarisoitujen fluoresenssiemissioiden mittaamiseksi
ES2051773T3 (es) Procedimiento para la obtencion de aralquil-4h,1,2,4-triazil derivados.
NO985775L (no) FremgangsmÕte til fremstilling av aminocyanacetamid
DE69604263D1 (de) Wärmeempfindliches Auszeignungsmaterial, das Phosphorsäurederivaten als Schmiermitteln enthält
ES2195309T3 (es) Derivados de tetrahidrofurano.
SU1515906A1 (ru) Способ определения интенсивности сверхслабого свечения цельной крови
KR970048709U (ko) 양액 재배용 베드벤치
ES2039270T3 (es) Derivados de gamma-l-glutamil-4-nitroanilida y procedimiento para determinar actividad de gamma-gtp utilizandolos.
IT1188716B (it) Rullo di strutturazione per la goffratura di nastri ricoperti per flocculazione
KR960011944U (ko) Ph측정용 온도조절장치

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 1058738

Country of ref document: ES