ES2170229T3 - Procedimiento de deteccion bioquimica asociado en linea a tecnicas de separacion de flujo. - Google Patents
Procedimiento de deteccion bioquimica asociado en linea a tecnicas de separacion de flujo.Info
- Publication number
- ES2170229T3 ES2170229T3 ES96911109T ES96911109T ES2170229T3 ES 2170229 T3 ES2170229 T3 ES 2170229T3 ES 96911109 T ES96911109 T ES 96911109T ES 96911109 T ES96911109 T ES 96911109T ES 2170229 T3 ES2170229 T3 ES 2170229T3
- Authority
- ES
- Spain
- Prior art keywords
- affinity molecule
- effluent
- line
- controlled amount
- detection
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000001514 detection method Methods 0.000 title abstract 5
- 238000000034 method Methods 0.000 title abstract 4
- 238000000926 separation method Methods 0.000 title 1
- 238000005194 fractionation Methods 0.000 abstract 4
- 239000003446 ligand Substances 0.000 abstract 4
- 150000001875 compounds Chemical class 0.000 abstract 2
- 238000006243 chemical reaction Methods 0.000 abstract 1
- 230000008878 coupling Effects 0.000 abstract 1
- 238000010168 coupling process Methods 0.000 abstract 1
- 238000005859 coupling reaction Methods 0.000 abstract 1
- 230000003993 interaction Effects 0.000 abstract 1
- 238000012216 screening Methods 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/84—Preparation of the fraction to be distributed
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/84—Preparation of the fraction to be distributed
- G01N2030/8429—Preparation of the fraction to be distributed adding modificating material
- G01N2030/8435—Preparation of the fraction to be distributed adding modificating material for chemical reaction
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
- G01N2030/8804—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 automated systems
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
- G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
- G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
- G01N2030/8827—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials involving nucleic acids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
- G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
- G01N2030/884—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample organic compounds
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
- Measurement Of The Respiration, Hearing Ability, Form, And Blood Characteristics Of Living Organisms (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
LA PRESENTE INVENCION SE REFIERE A UN METODO DE DETECCION EN CADENA, QUE COMPRENDE EL ACOPLAMIENTO EN CADENA DE UNA TECNICA BIOQUIMICA Y UNA TECNICA DE FRACCIONAMIENTO, COMPRENDIENDO DICHO METODO LA ADICION DE UNA CANTIDAD CONTROLADA DE UNA MOLECULA AFIN AL EFLUENTE DE LA FASE DE FRACCIONAMEINTO, DE FORMA QUE REACCIONE CON LOS ANALITOS CONTENIDOS EN EL EFLUENTE, LA ADICION POSTERIOR DE UNA CANTIDAD CONTROLADA DE UN LIGANDO DETECTABLE, CAPAZ DE UNIRSE A LA MOLECULA AFIN, Y LA DETECCION DEL COMPLEJO FORMADO ENTRE EL LIGANDO DETECTABLE Y LA MOLECULA AFIN. ADEMAS, LA INVENCION SE REFIERE A UN METODO EN CADENA PARA LA SELECCION DE COMPUESTOS EN FUNCION DE SU CAPACIDAD DE UNION A UNA MOLECULA AFIN CONOCIDA. DICHO METODO COMPRENDE UNA FASE DE UNA FASE DE FRACCIONAMIENTO QUE PROPORCIONA UN EFLUENTE, LA ADICION DE UNA CANTIDAD CONTROLADA DE DICHA MOLECULA AFIN AL EFLUENTE Y EL TRANSCURSO DE UN TIEMPO DE CONTACTO SUFICIENTE PARA PERMITIR UNA REACCION O INTERACCION ENTRE LOS COMPUESTOS PRESENTES EN ELEFLUENTE, LA ADICION POSTERIOR DE UNA CANTIDAD CONTROLADA DE UN LIGANDO DETECTABLE CAPAZ DE UNIRSE A LA MOLECULA AFIN Y LA DETECCION DEL COMPLEJO FORMADO ENTRE EL LIGANDO DETECTABLE Y LA MOLECULA AFIN.
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP95201029 | 1995-04-21 | ||
| EP95202987 | 1995-11-03 | ||
| EP96911109A EP0821790B1 (en) | 1995-04-21 | 1996-04-19 | On-line coupling of biochemical detection to continuous-flow separation techniques |
| PCT/NL1996/000173 WO1996033409A1 (en) | 1995-04-21 | 1996-04-19 | On-line coupling of biochemical detection to continuous-flow separation techniques |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2170229T3 true ES2170229T3 (es) | 2002-08-01 |
Family
ID=26139240
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES96911109T Expired - Lifetime ES2170229T3 (es) | 1995-04-21 | 1996-04-19 | Procedimiento de deteccion bioquimica asociado en linea a tecnicas de separacion de flujo. |
Country Status (10)
| Country | Link |
|---|---|
| EP (1) | EP0821790B1 (es) |
| JP (1) | JP3739399B2 (es) |
| AT (1) | ATE212441T1 (es) |
| AU (1) | AU5409296A (es) |
| DE (1) | DE69618776T2 (es) |
| DK (1) | DK0821790T3 (es) |
| ES (1) | ES2170229T3 (es) |
| PT (1) | PT821790E (es) |
| SI (1) | SI0821790T1 (es) |
| WO (1) | WO1996033409A1 (es) |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5183740A (en) * | 1990-02-23 | 1993-02-02 | The United States Of America As Represented By The Secretary Of The Navy | Flow immunosensor method and apparatus |
-
1996
- 1996-04-19 AU AU54092/96A patent/AU5409296A/en not_active Abandoned
- 1996-04-19 AT AT96911109T patent/ATE212441T1/de active
- 1996-04-19 WO PCT/NL1996/000173 patent/WO1996033409A1/en not_active Ceased
- 1996-04-19 DK DK96911109T patent/DK0821790T3/da active
- 1996-04-19 DE DE69618776T patent/DE69618776T2/de not_active Expired - Lifetime
- 1996-04-19 JP JP53164496A patent/JP3739399B2/ja not_active Expired - Fee Related
- 1996-04-19 SI SI9630440T patent/SI0821790T1/xx unknown
- 1996-04-19 PT PT96911109T patent/PT821790E/pt unknown
- 1996-04-19 ES ES96911109T patent/ES2170229T3/es not_active Expired - Lifetime
- 1996-04-19 EP EP96911109A patent/EP0821790B1/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| SI0821790T1 (en) | 2002-04-30 |
| JP3739399B2 (ja) | 2006-01-25 |
| WO1996033409A1 (en) | 1996-10-24 |
| DK0821790T3 (da) | 2002-05-13 |
| EP0821790A1 (en) | 1998-02-04 |
| DE69618776D1 (de) | 2002-03-14 |
| ATE212441T1 (de) | 2002-02-15 |
| JPH11509922A (ja) | 1999-08-31 |
| PT821790E (pt) | 2002-07-31 |
| AU5409296A (en) | 1996-11-07 |
| DE69618776T2 (de) | 2002-10-17 |
| EP0821790B1 (en) | 2002-01-23 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG2A | Definitive protection |
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