ES2171505T3 - Metodo turbidimetrico cinetico para medir endotoxina o (1-3)-beta-d-glucano. - Google Patents

Metodo turbidimetrico cinetico para medir endotoxina o (1-3)-beta-d-glucano.

Info

Publication number
ES2171505T3
ES2171505T3 ES95307614T ES95307614T ES2171505T3 ES 2171505 T3 ES2171505 T3 ES 2171505T3 ES 95307614 T ES95307614 T ES 95307614T ES 95307614 T ES95307614 T ES 95307614T ES 2171505 T3 ES2171505 T3 ES 2171505T3
Authority
ES
Spain
Prior art keywords
glucano
sample
mixture
horseshoe
present
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES95307614T
Other languages
English (en)
Inventor
Aya Takaoka
Masakazu Tsuchiya
Keiko Kawabe
Haruhisa Ijiri
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fujifilm Wako Pure Chemical Corp
Original Assignee
Wako Pure Chemical Industries Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wako Pure Chemical Industries Ltd filed Critical Wako Pure Chemical Industries Ltd
Application granted granted Critical
Publication of ES2171505T3 publication Critical patent/ES2171505T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/579—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving limulus lysate

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

UN PROCESO PARA MEDIR LA CANTIDAD DE ENDOTOXINA O (1 --> 3){BE}-D-GLUCANO (DENOMINADO DE AHORA EN ADELANTE {BE}-GLUCANO) PRESENTE EN UNA MUESTRA, QUE CONSISTE EN MEZCLAR LA MUESTRA CON LISADO DE AMEBOCITO DE CANGREJOS DE HERRADURA EN PRESENCIA DE AL MENOS UN POLIMERO SOLUBLE EN AGUA SELECCIONADO DEL GRUPO QUE CONSISTE EN UN POLIETILENGLICOL, UN ALCOHOL POLIVINILICO, UNA METILCELULOSA Y UNA HIDROXIPROPILCELULOSA, APLICAR UNA LUZ A LA MEZCLA RESULTANTE, MEDIR EL TIEMPO REQUERIDO HASTA QUE UN GRADO DE VARIACION OPTICA DE LA MEZCLA RESULTANTE ALCANCE UN VALOR PREDETERMINADO TRAS LA MEZCLA DE LA MUESTRA CON EL LISADO DE AMEBOCITO, O TRAS HABER TRANSCURRIDO UN PERIODO DE TIEMPO PREDETERMINADO DESDE LA MEZCLA DE LA MUESTRA CON EL LISADO DE AMEBOCITO, Y DETERMINAR LA CANTIDAD DE ENDOTOXINA O DE {BE}GLUCANO PRESENTE EN LA MUESTRA EN BASE A LA RELACION ENTRE DICHO TIEMPO Y LA CANTIDAD DE ENDOTOXINA Y {BE}-GLUCANO; Y TAMBIEN UN REACTIVO PARA MEDIR LA CANTIDAD DE ENDOTOXINA O {BE}GLUCANO PRESENTE EN UNA MUESTRA, QUE CONTIENE LISADO DE AMEBOCITO DE CANGREJOS DE HERRADURA Y AL MENOS UN POLIMERO SOLUBLE EN AGUA SELECCIONADO DEL GRUPO QUE CONSISTE EN UN POLIETILENGLICOL, UN ALCOHOL POLIVINILICO, UNA METILCELULOSA Y UNA HIDROXIPROPILCELULOSA.
ES95307614T 1994-10-31 1995-10-26 Metodo turbidimetrico cinetico para medir endotoxina o (1-3)-beta-d-glucano. Expired - Lifetime ES2171505T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP29059694 1994-10-31

Publications (1)

Publication Number Publication Date
ES2171505T3 true ES2171505T3 (es) 2002-09-16

Family

ID=17758060

Family Applications (1)

Application Number Title Priority Date Filing Date
ES95307614T Expired - Lifetime ES2171505T3 (es) 1994-10-31 1995-10-26 Metodo turbidimetrico cinetico para medir endotoxina o (1-3)-beta-d-glucano.

Country Status (6)

Country Link
US (1) US5637474A (es)
EP (1) EP0709681B1 (es)
CN (1) CN1081333C (es)
CA (1) CA2161210C (es)
DE (1) DE69525587T2 (es)
ES (1) ES2171505T3 (es)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5858401A (en) * 1996-01-22 1999-01-12 Sidmak Laboratories, Inc. Pharmaceutical composition for cyclosporines
US6270982B1 (en) 1997-10-09 2001-08-07 Charles River Laboratories Methods and compositions for the detection of bacterial endotoxins
JP4235279B2 (ja) * 1998-04-10 2009-03-11 生化学工業株式会社 新規酵素反応測定法
JP2004251807A (ja) * 2003-02-21 2004-09-09 Nipro Corp エンドトキシン測定用センサ、測定方法、診断方法、製造方法およびセンサ再生方法
JP5118849B2 (ja) * 2003-03-17 2013-01-16 チャールズ リバー ラボラトリーズ， インコーポレイテッド 微生物夾雑物の検出のための方法および組成物
CN100383528C (zh) * 2004-07-02 2008-04-23 中国科学院大连化学物理研究所 一种通过测定浊度值来进行细菌内毒素检查的方法
WO2007078268A2 (en) * 2004-12-02 2007-07-12 Charles River Laboratories, Inc. Methods and compositions for the detection and/or quantification of gram positive bacterial contaminants
ATE419526T1 (de) 2005-01-13 2009-01-15 Charles River Lab Inc Verfahren zur klassifizierung von mikroben in einer biologischen probe
WO2009137244A1 (en) * 2008-04-15 2009-11-12 Charles River Laboratories, Inc. Cartridge and method for sample analysis
JP5746614B2 (ja) 2008-04-15 2015-07-08 ザ ユナイテッド ステイツ オブ アメリカ、 アズ リプレゼンティッド バイ ザ セクレタリー オブ アグリカルチャー 澱粉含有穀物から得られる蛋白質濃縮物、その組成、製造方法及び使用
WO2010107068A1 (ja) * 2009-03-17 2010-09-23 和光純薬工業株式会社 β－グルカンの測定方法及びそれに用いられるβ－グルカン結合性蛋白質
JP5319842B2 (ja) * 2010-10-18 2013-10-16 徹 小幡 ゲル粒子測定試薬及びこれを用いた測定方法
CN102650593A (zh) * 2012-04-24 2012-08-29 湖南迪斯生物技术有限公司 一种内毒素真菌检测仪
CN102866255B (zh) * 2012-09-13 2014-12-24 莫水晶 一种人体液真菌(1,3)-β-D葡聚糖检测试剂盒及其应用方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4221865A (en) * 1978-10-06 1980-09-09 Baxter Travenol Laboratories, Inc. Method for determining endotoxin concentration
US4221866A (en) * 1978-10-06 1980-09-09 Baxter Travenol Laboratories, Inc. Method for determining endotoxin concentration
US4273557A (en) * 1980-03-31 1981-06-16 Mallinckrodt, Inc. Limulus lysate procedure for determining endotoxins
US4322217A (en) * 1980-06-27 1982-03-30 Mallinckrodt, Inc. Process for preparing Limulus lysate
ATE92630T1 (de) * 1984-06-27 1993-08-15 Wako Pure Chem Ind Ltd Geraet zur messung von endotoxin.
JPH0715474B2 (ja) * 1988-02-27 1995-02-22 和光純薬工業株式会社 エンドトキシンの測定法
JP3524120B2 (ja) * 1992-05-08 2004-05-10 生化学工業株式会社 前処理剤、前処理方法、前処理された試料による測定法、測定用キット及び試料の判定方法
JP2817607B2 (ja) * 1993-12-27 1998-10-30 和光純薬工業株式会社 エンドトキシンの安定化方法

Also Published As

Publication number Publication date
CN1081333C (zh) 2002-03-20
EP0709681A1 (en) 1996-05-01
DE69525587D1 (de) 2002-04-04
CA2161210C (en) 2005-08-02
US5637474A (en) 1997-06-10
DE69525587T2 (de) 2002-10-17
CN1132352A (zh) 1996-10-02
EP0709681B1 (en) 2002-02-27
CA2161210A1 (en) 1996-05-01

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