ES2175992T3 - Obtencion de polimeros a partir de componentes nucleotidicos y/o no nucleotidicos por via enzimatica. - Google Patents

Obtencion de polimeros a partir de componentes nucleotidicos y/o no nucleotidicos por via enzimatica.

Info

Publication number
ES2175992T3
ES2175992T3 ES99929296T ES99929296T ES2175992T3 ES 2175992 T3 ES2175992 T3 ES 2175992T3 ES 99929296 T ES99929296 T ES 99929296T ES 99929296 T ES99929296 T ES 99929296T ES 2175992 T3 ES2175992 T3 ES 2175992T3
Authority
ES
Spain
Prior art keywords
nucleotidic
enzymatic
components
obtaining polymers
polymers
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES99929296T
Other languages
English (en)
Inventor
Roxana Maria Havlina
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Larova Biochemie GmbH
Original Assignee
Larova Biochemie GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Larova Biochemie GmbH filed Critical Larova Biochemie GmbH
Application granted granted Critical
Publication of ES2175992T3 publication Critical patent/ES2175992T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/55—Design of synthesis routes, e.g. reducing the use of auxiliary or protecting groups

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Macromonomer-Based Addition Polymer (AREA)

Abstract

Procedimiento para la obtención de polímeros naturales y/o modificados con una secuencia definida de componentes nucleotídicos y/o no nucleotídicos, que comprende los siguientes pasos: a) unión de un cebador de la formula general p(dNp)m o p(rNp)m, pudiendo faltar, en caso dado, el grupo p en la posición 3¿ del cebador, con un soporte de la fórmula general (rM)ndN o (rM)ndU-rN, en los que p, dN, rN, m, rM, n y dU tienen los siguientes significados: p un grupo fosfato y/o fosforotioato y/o fosfonato, como preferentemente metilfosfonato y/o H-fosfonato, y/o fosforoamidato y/o formacetal y/o fosforoditioato y/o boranofosfato y/o fosforotriéster, dN un desoxiribonucleósido natural y/o modificado con bases y/o azúcares, y/o un grupo de enlace no nucleosídico apto para la unión, rN un ribonucleósido natural y/o modificado con bases y/o azúcares, y/o un grupo de enlace no nucleosídico apto para la unión, m un número entero de 1 o más, rM un ribonucleósido de purina y/o pirimidina, n un número entero de 1 o más. dU una desoxiuridina, pudiendo comprender el cebador de la fórmula general p(dNp)m o p(rNp)m también restos mixtos de dN y/o rN, con ayuda de una ARN-ligasa en presencia de cationes metálicos divalentes, no poseyendo el soporte ningún grupo protector en la posición 3¿; b) desprotección del producto de unión, en el caso de empleo del soporte de la fórmula general (rM)ndN mediante eliminación del grupo protector (rM)n en presencia de una RNasa, o bien eliminación del grupo protector (rM)ndU, en el caso de empleo del soporte de la fórmula general (rM)ndU-rN con ayuda de una uracil-ADN-glicosilasa y una endonucleasa apurínica, o eliminación de los citados grupos protectores en el intervalo alcalino; c) transferencia de un grupo terminal natural y/o modificado, de la fórmula general p definida anteriormente, al grupo 5¿-hidroxilo del producto de unión con ayuda de una polinucleótido-quinasa; d) en caso dado repetición de los pasos a)-c), y; e) en caso dado purificación del producto de unión obtenido en el paso c).
ES99929296T 1998-06-22 1999-06-22 Obtencion de polimeros a partir de componentes nucleotidicos y/o no nucleotidicos por via enzimatica. Expired - Lifetime ES2175992T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19827728 1998-06-22

Publications (1)

Publication Number Publication Date
ES2175992T3 true ES2175992T3 (es) 2002-11-16

Family

ID=7871626

Family Applications (1)

Application Number Title Priority Date Filing Date
ES99929296T Expired - Lifetime ES2175992T3 (es) 1998-06-22 1999-06-22 Obtencion de polimeros a partir de componentes nucleotidicos y/o no nucleotidicos por via enzimatica.

Country Status (11)

Country Link
US (1) US6329177B1 (es)
EP (1) EP1090137B1 (es)
JP (1) JP2002518059A (es)
AT (1) ATE215604T1 (es)
AU (1) AU753605B2 (es)
CA (1) CA2335562A1 (es)
DE (2) DE19928591A1 (es)
DK (1) DK1090137T3 (es)
ES (1) ES2175992T3 (es)
PT (1) PT1090137E (es)
WO (1) WO1999067413A1 (es)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6479262B1 (en) 2000-05-16 2002-11-12 Hercules, Incorporated Solid phase enzymatic assembly of polynucleotides
AP3396A (en) 2009-02-13 2015-08-31 Chem Inc X Methods of creating and screening DNA-encoded libraries
WO2012092540A1 (en) * 2010-12-30 2012-07-05 Ibis Biosciences, Inc. Methods for the synthesis of polyadenylic acid
EP2748357B1 (en) * 2011-09-07 2018-04-04 X-Chem, Inc. Methods for tagging dna-encoded libraries
SG10201907347YA (en) 2012-07-13 2019-09-27 X Chem Inc Dna-encoded libraries having encoding oligonucleotide linkages not readable by polymerases
CN117070512B (zh) * 2023-10-16 2024-04-26 吉林凯莱英医药化学有限公司 tRNA及其生物合成方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3300478A (en) * 1965-06-01 1967-01-24 Upjohn Co Arabinofuranosyl 2', 5'-and 3'-5'-dinucleoside phosphates and process therefor
JP2780572B2 (ja) 1991-09-13 1998-07-30 株式会社島津製作所 オリゴヌクレオチドの酵素的合成法及びオリゴヌクレオチドのプライマーとしての使用
US5516664A (en) * 1992-12-23 1996-05-14 Hyman; Edward D. Enzymatic synthesis of repeat regions of oligonucleotides
US5750341A (en) * 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
US5889136A (en) * 1995-06-09 1999-03-30 The Regents Of The University Of Colorado Orthoester protecting groups in RNA synthesis

Also Published As

Publication number Publication date
WO1999067413A1 (de) 1999-12-29
AU753605B2 (en) 2002-10-24
EP1090137B1 (de) 2002-04-03
PT1090137E (pt) 2002-08-30
DK1090137T3 (da) 2002-06-17
JP2002518059A (ja) 2002-06-25
DE59901131D1 (de) 2002-05-08
CA2335562A1 (en) 1999-12-29
US6329177B1 (en) 2001-12-11
DE19928591A1 (de) 1999-12-23
EP1090137A1 (de) 2001-04-11
AU4614499A (en) 2000-01-10
ATE215604T1 (de) 2002-04-15

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