ES2179874T3 - Procedimiento y articulos para producir metabolitos secundarios de celulas vegetales viables inmovilizadas en una matriz porosa. - Google Patents
Procedimiento y articulos para producir metabolitos secundarios de celulas vegetales viables inmovilizadas en una matriz porosa.Info
- Publication number
- ES2179874T3 ES2179874T3 ES95920202T ES95920202T ES2179874T3 ES 2179874 T3 ES2179874 T3 ES 2179874T3 ES 95920202 T ES95920202 T ES 95920202T ES 95920202 T ES95920202 T ES 95920202T ES 2179874 T3 ES2179874 T3 ES 2179874T3
- Authority
- ES
- Spain
- Prior art keywords
- matrix
- pct
- sio2
- cells
- viable
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000011159 matrix material Substances 0.000 title abstract 9
- 229930000044 secondary metabolite Natural products 0.000 title abstract 2
- 235000013311 vegetables Nutrition 0.000 title 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 abstract 7
- 210000004027 cell Anatomy 0.000 abstract 6
- 229910052681 coesite Inorganic materials 0.000 abstract 3
- 229910052906 cristobalite Inorganic materials 0.000 abstract 3
- 239000000377 silicon dioxide Substances 0.000 abstract 3
- 235000012239 silicon dioxide Nutrition 0.000 abstract 3
- 229910052682 stishovite Inorganic materials 0.000 abstract 3
- 229910052905 tridymite Inorganic materials 0.000 abstract 3
- 239000002243 precursor Substances 0.000 abstract 2
- 239000012159 carrier gas Substances 0.000 abstract 1
- 230000003833 cell viability Effects 0.000 abstract 1
- 239000011248 coating agent Substances 0.000 abstract 1
- 238000000576 coating method Methods 0.000 abstract 1
- 239000007789 gas Substances 0.000 abstract 1
- 210000001822 immobilized cell Anatomy 0.000 abstract 1
- 230000003100 immobilizing effect Effects 0.000 abstract 1
- 229910010272 inorganic material Inorganic materials 0.000 abstract 1
- 239000011147 inorganic material Substances 0.000 abstract 1
- 229910052809 inorganic oxide Inorganic materials 0.000 abstract 1
- 230000002452 interceptive effect Effects 0.000 abstract 1
- 239000002207 metabolite Substances 0.000 abstract 1
- 239000011148 porous material Substances 0.000 abstract 1
- 229920006395 saturated elastomer Polymers 0.000 abstract 1
- 239000000725 suspension Substances 0.000 abstract 1
- 230000035899 viability Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/04—Enzymes or microbial cells immobilised on or in an organic carrier entrapped within the carrier, e.g. gel or hollow fibres
Landscapes
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Dispersion Chemistry (AREA)
- Inorganic Chemistry (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
SE DESCRIBE UN PROCEDIMIENTO PARA PRODUCIR METABOLITOS SECUNDARIOS DE CELULAS VEGETALES VIABLES, QUE INCLUYEN LAS ETAPAS DE: (A) PREPARAR UN SOPORTE QUE INCLUYE UNA MATRIZ SUSTANCIALMENTE UNIFORME Y POROSA DE MATERIAL INORGANICO, QUE POSEE UNA RESISTENCIA A LA TRACCION DE AL MENOS 500 MPA; (B) INTRODUCIR UN CULTIVO DE CELULAS VEGETALES VIABLES EN LOS POROS DE DICHA MATRIZ; (C) ATRAPAR LAS CELULAS VEGETALES MEDIANTE EL REVESTIMIENTO DE LA MATRIZ CON UN SOL O UNA SUSPENSION COLOIDAL QUE NO INTERFIERA CON LA VIABILIDAD DE LAS CELULAS; (D) INMOVILIZAR LAS CELULAS ATRAPADAS EN LA MATRIZ CON UN GAS REACTIVO QUE INCLUYE UN GAS VEHICULO SATURADO CON SIO SUB,2} VOLATIL O PRECURSORES DE SIO SUB,2} MODIFICADOS ORGANICOS. LA MATRIZ PUEDE SER UN SIO SUB,2} O MATRICES DE OXIDO INORGANICO, EN LAS CUALES LA PROPORCION EN PESO ENTRE LA CARGA CELULAR Y EL MATERIAL INORGANICO VARIA ENTRE 1 X 10 SUP,-4} Y 1 X 10 SUP,-2}. LAS CELULAS INMOVILIZADAS NO ENTRAN EN SOLUCION DURANTE UN PERIODO DE 6 MESES Y MANTIENENSU VIABILIDAD MIENTRAS QUE PRODUCEN METABOLITOS SECUNDARIOS.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/IT1995/000083 WO1996036703A1 (en) | 1995-05-18 | 1995-05-18 | Process and articles for producing secondary metabolites of viable plant cells immobilized in a porous matrix |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2179874T3 true ES2179874T3 (es) | 2003-02-01 |
Family
ID=11332468
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES95920202T Expired - Lifetime ES2179874T3 (es) | 1995-05-18 | 1995-05-18 | Procedimiento y articulos para producir metabolitos secundarios de celulas vegetales viables inmovilizadas en una matriz porosa. |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US5998162A (es) |
| EP (1) | EP0827535B1 (es) |
| JP (1) | JP3662026B2 (es) |
| AT (1) | ATE221572T1 (es) |
| AU (1) | AU2574095A (es) |
| BR (1) | BR9510598A (es) |
| CA (1) | CA2219491A1 (es) |
| DE (1) | DE69527657T2 (es) |
| ES (1) | ES2179874T3 (es) |
| IL (1) | IL118220A (es) |
| WO (1) | WO1996036703A1 (es) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6214593B1 (en) * | 1996-05-28 | 2001-04-10 | Biosil A.G. | Encapsulation of supported animal cells using gas-phase inorganic alkoxides |
| DE10016554A1 (de) * | 2000-04-03 | 2001-10-18 | Rootec Ges Fuer Bioaktive Wirk | Vorrichtung zum Kultivieren von pflanzlichen oder tierischen Gewebekulturen |
| DE10163640A1 (de) * | 2001-12-21 | 2003-07-03 | Arno Kromminga | Nachweis von Wechselwirkungen zwischen mindestens zwei spezifischen Biomolekülen |
| US7229808B2 (en) | 2002-01-18 | 2007-06-12 | Silbiotec Due S.A. | Bioreactor |
| WO2009158502A1 (en) * | 2008-06-25 | 2009-12-30 | Purdue Research Foundation | Encapsulation of living cells within an aerosolized sol-gel matrix |
| AU2010276255A1 (en) | 2009-07-21 | 2012-03-01 | Purdue Research Foundation | Cell-mediated silica sol-gel encapsulation of living cells and tissues |
| WO2012074502A1 (en) * | 2010-11-29 | 2012-06-07 | Chayil Technologies, Llc | Secondary metabolite stimulation in photoautotrophic cultures |
| DE102013208227A1 (de) * | 2013-05-06 | 2014-11-06 | Siemens Aktiengesellschaft | Photobioreaktor für immobilisierte Mikroorganismen |
| US11299700B1 (en) | 2021-02-19 | 2022-04-12 | Acequia Biotechnology, Llc | Bioreactor containers and methods of growing hairy roots using the same |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4845054A (en) * | 1985-06-14 | 1989-07-04 | Focus Semiconductor Systems, Inc. | Low temperature chemical vapor deposition of silicon dioxide films |
| GB2185998A (en) * | 1986-01-30 | 1987-08-05 | Pilkington Brothers Plc | Improvements in or relating to the growth of plant tissue cultures |
| EP0267470A1 (en) * | 1986-11-03 | 1988-05-18 | Manville Corporation | Porous glass fiber mats for attachment of cells and biologically active substances |
| US5041138A (en) * | 1986-11-20 | 1991-08-20 | Massachusetts Institute Of Technology | Neomorphogenesis of cartilage in vivo from cell culture |
-
1995
- 1995-05-18 JP JP53468496A patent/JP3662026B2/ja not_active Expired - Fee Related
- 1995-05-18 EP EP95920202A patent/EP0827535B1/en not_active Expired - Lifetime
- 1995-05-18 AU AU25740/95A patent/AU2574095A/en not_active Abandoned
- 1995-05-18 DE DE69527657T patent/DE69527657T2/de not_active Expired - Fee Related
- 1995-05-18 BR BR9510598-0A patent/BR9510598A/pt unknown
- 1995-05-18 AT AT95920202T patent/ATE221572T1/de not_active IP Right Cessation
- 1995-05-18 ES ES95920202T patent/ES2179874T3/es not_active Expired - Lifetime
- 1995-05-18 WO PCT/IT1995/000083 patent/WO1996036703A1/en not_active Ceased
- 1995-05-18 US US08/952,104 patent/US5998162A/en not_active Expired - Fee Related
- 1995-05-18 CA CA002219491A patent/CA2219491A1/en not_active Abandoned
-
1996
- 1996-05-10 IL IL11822096A patent/IL118220A/xx not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| US5998162A (en) | 1999-12-07 |
| BR9510598A (pt) | 1999-11-30 |
| WO1996036703A1 (en) | 1996-11-21 |
| DE69527657D1 (de) | 2002-09-05 |
| DE69527657T2 (de) | 2003-04-03 |
| EP0827535B1 (en) | 2002-07-31 |
| IL118220A (en) | 2000-07-16 |
| IL118220A0 (en) | 1996-09-12 |
| JP3662026B2 (ja) | 2005-06-22 |
| AU2574095A (en) | 1996-11-29 |
| ATE221572T1 (de) | 2002-08-15 |
| CA2219491A1 (en) | 1996-11-21 |
| JPH11505120A (ja) | 1999-05-18 |
| EP0827535A1 (en) | 1998-03-11 |
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