ES2179909T3 - Cebadores de oligonucleotidos para la amplificacion del acido nucleico del vhc. - Google Patents

Cebadores de oligonucleotidos para la amplificacion del acido nucleico del vhc.

Info

Publication number
ES2179909T3
ES2179909T3 ES96118704T ES96118704T ES2179909T3 ES 2179909 T3 ES2179909 T3 ES 2179909T3 ES 96118704 T ES96118704 T ES 96118704T ES 96118704 T ES96118704 T ES 96118704T ES 2179909 T3 ES2179909 T3 ES 2179909T3
Authority
ES
Spain
Prior art keywords
nucleic acid
primers
amplification
hcv
oligonucleotide primers
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES96118704T
Other languages
English (en)
Inventor
Sue Yen Tsang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
F Hoffmann La Roche AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Application granted granted Critical
Publication of ES2179909T3 publication Critical patent/ES2179909T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/706—Specific hybridization probes for hepatitis
    • C12Q1/707—Specific hybridization probes for hepatitis non-A, non-B Hepatitis, excluding hepatitis D
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Communicable Diseases (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Virology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

LA PRESENTE INVENCION PROPORCIONA CEBADORES MEJORADOS PARA LA AMPLIFICACION DE UNA SECUENCIA DE ACIDO NUCLEICO DEL VIRUS DE LA HEPATITIS C (HCV), MEDIANTE REACCION EN CADENA DE LA POLIMERASA (PCR). LOS CEBADORES Y LOS METODOS DE AMPLIFICACION DE LA INVENCION, PERMITEN LA DETECCION DE HCV, CON UNA SENSIBILIDAD CONSIDERABLEMENTE MAYOR. LA INVENCION PROPORCIONA ASIMISMO EQUIPAMIENTOS QUE COMPRENDEN DICHOS CEBADORES, Y METODOS DE AMPLIFICACION DE UN ACIDO NUCLEICO DEL VIRUS DE LA HEPATITIS C (HCV) EN UNA MUESTRA, UTILIZANDO DICHOS CEBADORES. SE PROPORCIONAN TAMBIEN METODOS PARA DETECTAR ACIDO NUCLEICO DE HCV EN UNA MUESTRA, EN LA QUE DICHO ACIDO NUCLEICO HA SIDO AMPLIFICADO UTILIZANDO LOS CEBADORES MENCIONADOS ANTERIORMENTE.
ES96118704T 1995-11-29 1996-11-21 Cebadores de oligonucleotidos para la amplificacion del acido nucleico del vhc. Expired - Lifetime ES2179909T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US773995P 1995-11-29 1995-11-29

Publications (1)

Publication Number Publication Date
ES2179909T3 true ES2179909T3 (es) 2003-02-01

Family

ID=21727873

Family Applications (1)

Application Number Title Priority Date Filing Date
ES96118704T Expired - Lifetime ES2179909T3 (es) 1995-11-29 1996-11-21 Cebadores de oligonucleotidos para la amplificacion del acido nucleico del vhc.

Country Status (8)

Country Link
US (1) US5837442A (es)
EP (1) EP0776981B1 (es)
JP (2) JP3814352B2 (es)
AT (1) ATE220726T1 (es)
CA (1) CA2191182C (es)
DE (1) DE69622347T2 (es)
DK (1) DK0776981T3 (es)
ES (1) ES2179909T3 (es)

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7070925B1 (en) * 1996-07-16 2006-07-04 Gen-Probe Incorporated Method for determining the presence of an RNA analyte in a sample using a modified oligonucleotide probe
DE69827060T2 (de) * 1997-03-20 2005-03-24 F. Hoffmann-La Roche Ag Modifizierte Primer
US6638714B1 (en) * 1999-02-03 2003-10-28 Ortho-Clinical Diagnostics, Inc. Oligonucleotide primers for efficient detection of hepatitis C virus (HCV) and methods of use thereof
DE60015210T2 (de) * 1999-02-03 2006-02-16 Ortho-Clinical Diagnostics, Inc. Oligonukleotid Primer zur effizienten reversen Transkription von Hepatitis C Virus (HCV) RNS und Verfahren zu deren Verwendung
DE60045059D1 (de) * 1999-04-20 2010-11-18 Nat Inst Of Advanced Ind Scien Verfahren und Sonden zur Bestimmung der Konzentration von Nukleinsäure-Molekülen und Verfahren zur Analyse der gewonnenen Daten
US6586584B2 (en) 2001-01-29 2003-07-01 Becton, Dickinson And Company Sequences and methods for detection of Hepatitis C virus
WO2002070751A1 (en) * 2001-03-02 2002-09-12 University Of Pittsburgh Of The Commonwealth System Of Higher Education Pcr method
EP1236804A1 (en) * 2001-03-02 2002-09-04 Boehringer Mannheim Gmbh A method for determination of a nucleic acid using a control
WO2002090572A2 (en) * 2001-05-09 2002-11-14 Third Wave Technologies, Inc. Nucleic acid detection in pooled samples
KR100440852B1 (ko) * 2001-05-10 2004-07-19 주식회사 바이로메드 레트로바이러스에 특이적인 올리고뉴클레오티드 프라이머및 이를 이용한 레트로바이러스 농도 측정방법
US6946245B2 (en) 2001-12-04 2005-09-20 Quest Diagnostics Investments Incorporated Oligonucleotides and methods for detecting hepatitis C viral nucleic acids
DE60330877D1 (de) * 2002-07-26 2010-02-25 Abbott Lab Verfahren zum nachweis und zur quantifizierung von hepatitis-c-virus
WO2004094986A2 (en) 2003-04-16 2004-11-04 Handylab, Inc. System and method for electrochemical detection of biological compounds
US7824857B2 (en) * 2003-12-02 2010-11-02 Musc Foundation For Research Development Methods and compositions for diagnosing epithelial cell cancer
WO2005084254A2 (en) * 2004-02-27 2005-09-15 Musc Foundation For Research Development Enhanced detection of rna using a panel of truncated gene-specific primers for reverse transcription
EP2272987A3 (en) * 2004-07-09 2012-04-11 University of Pittsburgh of the Commonwealth System of Higher Education Identification of markers in esophageal cancer, colon cancer, head and neck cancer and melanoma
JP2008515393A (ja) * 2004-09-20 2008-05-15 ユニバーシティ オブ ピッツバーグ オブ ザ コモンウェルス システム オブ ハイヤー エデュケイション 複数モードの多重化反応消去方法
WO2006085328A2 (en) * 2005-02-14 2006-08-17 Mor Research Applications Ltd. Method and kit for diagnosing and predicting hepatitis c virus-related neoplastic transformation
US7919242B2 (en) * 2005-06-30 2011-04-05 Roche Molecular Systems, Inc. Light emission modifiers and their uses in nucleic acid detection, amplification and analysis
WO2007011901A2 (en) * 2005-07-15 2007-01-25 Applera Corporation Hot start reverse transcription by primer design
CA2571904A1 (en) * 2006-02-15 2007-08-15 Fio Corporation System and method of detecting pathogens
US8119352B2 (en) * 2006-06-20 2012-02-21 Cepheld Multi-stage amplification reactions by control of sequence replication times
CA2580589C (en) 2006-12-19 2016-08-09 Fio Corporation Microfluidic detection system
US8360321B2 (en) 2007-04-02 2013-01-29 Fio Corporation System and method of deconvolving multiplexed fluorescence spectral signals generated by quantum dot optical coding technology
JP2010530912A (ja) 2007-06-22 2010-09-16 フィオ コーポレイション 量子ドットをドープしたポリマーマイクロビーズの製造システム及び方法
WO2009006739A1 (en) 2007-07-09 2009-01-15 Fio Corporation Systems and methods for enhancing fluorescent detection of target molecules in a test sample
WO2009028654A1 (ja) * 2007-08-31 2009-03-05 National University Corporation Tottori University ＴＥＲＴ遺伝子のｃＤＮＡの製造、それを用いた核酸増幅法、検出方法、それらに用いるプライマー、及び、それらを用いた腫瘍の診断キット
CN101861203B (zh) 2007-10-12 2014-01-22 Fio公司 用于形成浓缩体积的微珠的流动聚焦方法和系统以及形成的微珠
US9792809B2 (en) 2008-06-25 2017-10-17 Fio Corporation Bio-threat alert system
BRPI0917839A2 (pt) 2008-08-29 2015-11-24 Fio Corp dispositivo de teste de diagnóstico portátil de uso único, e um sistema associado e método para teste de amostras de teste ambientais e biológicas
CN104374932A (zh) 2009-01-13 2015-02-25 Fio公司 与电子设备和快速诊断测试中的测试盒结合使用的手持诊断测试设备

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4458066A (en) 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US5322770A (en) 1989-12-22 1994-06-21 Hoffman-Laroche Inc. Reverse transcription with thermostable DNA polymerases - high temperature reverse transcription
US5310652A (en) 1986-08-22 1994-05-10 Hoffman-La Roche Inc. Reverse transcription with thermostable DNA polymerase-high temperature reverse transcription
BR8807310A (pt) 1987-11-18 1990-03-13 Chiron Corp Polinucleotidio,polinucleotidio recombinante,vetor recombinante,celula hospedeira,sistema de expressao recombinante,celula transformada,polipeptidio,hcv,preparacao de polipeptidio,polipeptidio de hcv,polipeptidio purificado,polipeptidio recombinante,polipeptidio de fusao,anticorpo monoclonal,preparacao purificada de anticorpos policlonais,particula,sonda de polinucleotidio,kit anticorpo para epetopo de hcv,metodo para producao de polipeptidio,metodo para deteccao de acidos nucleicos de hcv,imunoensaio para detectar antigeno de hcv,imunoensaio para detectar anticorpos dirigidos contra antigeno de hcv,vacina para tratamento de infeccao de hcv,celula,celula infectada com hcv,metodo para producao de anticorpos para hcv e metodo para isolar cdna derivado do genoma de um agente infeccioso nao identificado
HU225068B1 (en) 1989-03-17 2006-05-29 Chiron Corp Process for producing diagnostics and vaccine of nanbh
JP2656996B2 (ja) 1989-05-18 1997-09-24 カイロン コーポレイション Ｎａｎｂｖの診断用薬
US5035996A (en) 1989-06-01 1991-07-30 Life Technologies, Inc. Process for controlling contamination of nucleic acid amplification reactions
WO1991012342A1 (en) 1990-02-16 1991-08-22 F. Hoffmann-La Roche Ag Improvements in the specificity and convenience of the polymerase chain reaction
ES2128323T3 (es) 1990-07-24 1999-05-16 Hoffmann La Roche Reduccion de la amplificacion no-especifica durante la amplificacion in vitro del acido nucleico empleando bases de acido nucleico modificadas.
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
DE69128520T2 (de) 1990-10-31 1998-07-09 Tosoh Corp Verfahren zum Nachweis oder Quantifizierung von Zielnukleinsäuren
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
DE69223562T2 (de) * 1991-08-27 1998-06-04 Hoffmann La Roche Verfahren und Reagenzien zum Nachweis von Hepatitis C
AU4387193A (en) * 1992-05-29 1993-12-30 Abbott Laboratories Ligase chain reaction starting with rna sequences
ATE205259T1 (de) * 1993-07-01 2001-09-15 Hoffmann La Roche Reagenz und verfahren für reverse transkription bei hoher temperatur, verbunden mit einer polymerase-kettenreaktion
AU7718594A (en) * 1993-09-03 1995-03-22 United States Of America As Represented By The Secretary Department Of Health And Human Services Method and compositions for primer specific solid-phase detection of pcr products
US5491063A (en) 1994-09-01 1996-02-13 Hoffmann-La Roche Inc. Methods for in-solution quenching of fluorescently labeled oligonucleotide probes
US5571673A (en) 1994-11-23 1996-11-05 Hoffmann-La Roche Inc. Methods for in-solution quenching of fluorescently labeled oligonucleotide probes
US9105210B2 (en) 2012-06-29 2015-08-11 Microsoft Technology Licensing, Llc Multi-node poster location

Also Published As

Publication number Publication date
CA2191182A1 (en) 1997-05-30
CA2191182C (en) 2004-09-21
JPH09163991A (ja) 1997-06-24
JP3814352B2 (ja) 2006-08-30
JP3903059B2 (ja) 2007-04-11
ATE220726T1 (de) 2002-08-15
JP2006020642A (ja) 2006-01-26
EP0776981A3 (en) 1998-08-19
DK0776981T3 (da) 2002-11-11
EP0776981A2 (en) 1997-06-04
DE69622347T2 (de) 2003-02-13
EP0776981B1 (en) 2002-07-17
DE69622347D1 (de) 2002-08-22
US5837442A (en) 1998-11-17

Similar Documents

Publication Publication Date Title
ATE220726T1 (de) Oligonukleotid-primer für amplifizierung von hcv- nukleinsäure
AU665952B2 (en) Methods and reagents for hepatitis C detection
NO20000536D0 (no) Oligonukleotidprimere for effektiv deteksjon av Hepatitis C- virus (HCV) og metoder for anvendelse derav
DE69627408D1 (de) Primers und Sonden zum Nachweis von HIV
AU650055B2 (en) Nucleotide sequences useful as type-specific probes, PCR primers and LCR probes for the amplification and detection of human papilloma virus, and related kits and methods
CN112852921A (zh) 一种基于即时检测试纸条的核酸检测方法、检测探针及其试剂盒
ATE197481T1 (de) Verfahren und reagenzien zur typisierung von hla- genen der klasse i
DE60015210D1 (de) Oligonukleotid Primer zur effizienten reversen Transkription von Hepatitis C Virus (HCV) RNS und Verfahren zu deren Verwendung
RU93013954A (ru) Способ обнаружения вируса иммунодефицита