ES2182428T3 - Procedimiento para la preparacion por via enzimatica de antibioticos de betalactamas con recuperacion del producto a un ph de 1 en presencia de penicilamidasa inmovilizada. - Google Patents
Procedimiento para la preparacion por via enzimatica de antibioticos de betalactamas con recuperacion del producto a un ph de 1 en presencia de penicilamidasa inmovilizada.Info
- Publication number
- ES2182428T3 ES2182428T3 ES99109414T ES99109414T ES2182428T3 ES 2182428 T3 ES2182428 T3 ES 2182428T3 ES 99109414 T ES99109414 T ES 99109414T ES 99109414 T ES99109414 T ES 99109414T ES 2182428 T3 ES2182428 T3 ES 2182428T3
- Authority
- ES
- Spain
- Prior art keywords
- immobilized
- way
- procedure
- antibiotics
- enzyme
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000003242 anti bacterial agent Substances 0.000 title abstract 2
- 229940088710 antibiotic agent Drugs 0.000 title abstract 2
- 238000000034 method Methods 0.000 title abstract 2
- 230000002255 enzymatic effect Effects 0.000 title 1
- 238000011084 recovery Methods 0.000 title 1
- 108010093096 Immobilized Enzymes Proteins 0.000 abstract 3
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 abstract 2
- 239000003153 chemical reaction reagent Substances 0.000 abstract 2
- 239000000203 mixture Substances 0.000 abstract 2
- 239000007787 solid Substances 0.000 abstract 2
- -1 AMOXYCLINE Chemical compound 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 239000002253 acid Substances 0.000 abstract 1
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 abstract 1
- 229960000723 ampicillin Drugs 0.000 abstract 1
- 239000003782 beta lactam antibiotic agent Substances 0.000 abstract 1
- 230000003115 biocidal effect Effects 0.000 abstract 1
- 239000003054 catalyst Substances 0.000 abstract 1
- QYIYFLOTGYLRGG-GPCCPHFNSA-N cefaclor Chemical compound C1([C@H](C(=O)N[C@@H]2C(N3C(=C(Cl)CS[C@@H]32)C(O)=O)=O)N)=CC=CC=C1 QYIYFLOTGYLRGG-GPCCPHFNSA-N 0.000 abstract 1
- 229960005361 cefaclor Drugs 0.000 abstract 1
- ZAIPMKNFIOOWCQ-UEKVPHQBSA-N cephalexin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@@H]3N(C2=O)C(=C(CS3)C)C(O)=O)=CC=CC=C1 ZAIPMKNFIOOWCQ-UEKVPHQBSA-N 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
- 238000001914 filtration Methods 0.000 abstract 1
- 238000011534 incubation Methods 0.000 abstract 1
- 230000007935 neutral effect Effects 0.000 abstract 1
- 239000002245 particle Substances 0.000 abstract 1
- 230000007928 solubilization Effects 0.000 abstract 1
- 238000005063 solubilization Methods 0.000 abstract 1
- 239000002132 β-lactam antibiotic Substances 0.000 abstract 1
- 229940124586 β-lactam antibiotics Drugs 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P37/00—Preparation of compounds having a 4-thia-1-azabicyclo [3.2.0] heptane ring system, e.g. penicillin
- C12P37/04—Preparation of compounds having a 4-thia-1-azabicyclo [3.2.0] heptane ring system, e.g. penicillin by acylation of the substituent in the 6 position
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P35/00—Preparation of compounds having a 5-thia-1-azabicyclo [4.2.0] octane ring system, e.g. cephalosporin
- C12P35/04—Preparation of compounds having a 5-thia-1-azabicyclo [4.2.0] octane ring system, e.g. cephalosporin by acylation of the substituent in the 7 position
Landscapes
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Cephalosporin Compounds (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
LOS ANTIBIOTICOS DE BETALACTAMA AMPICILINA, AMOXICILINA, CEFALEXINA, CEFACLOR, CEFADROXILO, SE PREPARAN MEDIANTE BIOCATALISIS A PARTIR DE SU NUCLEO DE AMINOBETALACTAMA Y DE LOS CORRESPONDIENTES COMPONENTES DE CADENAS LATERALES ACTIVADOS. COMO CATALIZADOR SE UTILIZA AQUI PREFERIBLEMENTE LA ENZIMA PENICILINAMIDASA QUE ESTA INMOVILIZADA DE MANERA COVALENTE EN PARTICULAS DE SOPORTE INSOLUBLES. CUANDO SE UTILIZAN GRANDES CONCENTRACIONES DE LOS REACTIVOS PARA HACER ESTE PROCEDIMIENTO DE MANERA INDUSTRIAL SE PRECIPITA EL PRODUCTO OBTENIDO, ES DECIR EL ANTIBIOTICO DE BETALACTAMA, PRINCIPALMENTE EN FORMA SOLIDA SE SACA DE LA MEZCLA, Y SE PUEDE SEPARAR MUY DIFICILMENTE DEL BIOCATALIZADOR QUE TAMBIEN SE ENCUENTRA EN FORMA SOLIDA. LA ADICION DE ACIDO SULFURICO A LA MEZCLA REACTIVA Y EN PRESENCIA DE LA ENZIMA INMOVILIZADA DE MANERA COVALENTE NOS LLEVA HASTA UN PH DEL 1,0, LO QUE PERMITE LA SOLUBILIZACION DE LOS ANTIBIOTICOS DE BETALACTAMA ANTES CITADOS. DE ESTA MANERA SE PUEDEN SEPARAR POR FILTRACION FACILMENTE DE LA ENZIMA INMOVILIZADA. LA ENZIMA INMOVILIZADA INACTIVADA EN ESTE TRATAMIENTO SE PUEDE REGENERAR MEDIANTE INCUBACION EN UN TAMPON NEUTRO EN UNA CANTIDAD DE MAS DEL 90 % EN RELACION CON SU ACTIVIDAD Y SU ESTABILIDAD OPERACIONAL. DE ESTA MANERA SE PUEDE UTILIZAR LA ENZIMA INMOVILIZADA INCLUSO DESPUES DE LA ACCION DEL ACIDO VARIAS VECES, LO QUE SIGNIFICA UN AVANCE IMPORTANTE TECNICO Y ECONOMICO.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19823332A DE19823332C2 (de) | 1998-05-26 | 1998-05-26 | Verfahren zur enzymatischen Herstellung von Betalactam-Antibiotika |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2182428T3 true ES2182428T3 (es) | 2003-03-01 |
Family
ID=7868866
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES99109414T Expired - Lifetime ES2182428T3 (es) | 1998-05-26 | 1999-05-11 | Procedimiento para la preparacion por via enzimatica de antibioticos de betalactamas con recuperacion del producto a un ph de 1 en presencia de penicilamidasa inmovilizada. |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US6218138B1 (es) |
| EP (1) | EP0960945B1 (es) |
| AT (1) | ATE223496T1 (es) |
| DE (1) | DE19823332C2 (es) |
| ES (1) | ES2182428T3 (es) |
| TR (1) | TR199901164A3 (es) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE10029671A1 (de) * | 2000-06-23 | 2002-01-10 | Frieder Schauer | Biotransformation von biologisch aktiven Verindungen aus verschiedenen chemischen Stoffklassen mittels der Enzyme Laccase und Manganperoxidase |
| ITMI20030042A1 (it) * | 2003-01-14 | 2004-07-15 | Univ Degli Studi Trieste | Processo di sintesi enzimatica di antibiotici b lattamici. |
| JP4915732B2 (ja) * | 2006-12-15 | 2012-04-11 | 花王株式会社 | 固定化酵素を用いた有用物質の製造方法 |
| DE202008003248U1 (de) | 2008-03-07 | 2008-05-08 | Eurofilters Holding N.V. | Staubsaugerfilterbeutel |
| ES2440722T3 (es) | 2008-03-07 | 2014-01-30 | Eurofilters Holding N.V. | Bolsa de filtro de aspiradora |
| KR102869384B1 (ko) | 2017-07-07 | 2025-10-15 | 에피센트알엑스, 인코포레이티드 | 치료제의 비경구 투여를 위한 조성물 |
| CN109682913A (zh) * | 2019-01-16 | 2019-04-26 | 徐州立兴佳正医药科技有限公司 | 一种液质联用测定血浆中头孢羟氨苄浓度的方法 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4250260A (en) * | 1979-08-22 | 1981-02-10 | Uop Inc. | Regeneration of an immobilized enzyme system |
| DE3909018A1 (de) * | 1989-03-18 | 1990-09-20 | Roehm Gmbh | Protein-reinigungsverfahren |
| ES2059285T1 (es) * | 1991-12-19 | 1994-11-16 | Novo Nordisk As | Un metodo mejorado para la preparacion de ciertos antibioticos beta-lactamicos. |
| WO1994017800A1 (en) * | 1993-02-15 | 1994-08-18 | Gist-Brocades B.V. | AN IMPROVED β-LACTAM ANTIBIOTIC COMPOSITION |
| US6048708A (en) * | 1994-07-18 | 2000-04-11 | Gist-Brocades B.V. | Process for preparation of β-lactams at constantly high concentration of reactants |
| BE1009071A3 (nl) * | 1995-02-02 | 1996-11-05 | Dsm Nv | Werkwijze voor de bereiding van een beta-lactam antibioticum. |
-
1998
- 1998-05-26 DE DE19823332A patent/DE19823332C2/de not_active Expired - Fee Related
-
1999
- 1999-05-11 EP EP99109414A patent/EP0960945B1/de not_active Expired - Lifetime
- 1999-05-11 AT AT99109414T patent/ATE223496T1/de not_active IP Right Cessation
- 1999-05-11 ES ES99109414T patent/ES2182428T3/es not_active Expired - Lifetime
- 1999-05-26 TR TR1999/01164A patent/TR199901164A3/tr unknown
- 1999-05-26 US US09/318,732 patent/US6218138B1/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| US6218138B1 (en) | 2001-04-17 |
| EP0960945A2 (de) | 1999-12-01 |
| DE19823332C2 (de) | 2000-09-07 |
| TR199901164A2 (xx) | 1999-12-21 |
| EP0960945B1 (de) | 2002-09-04 |
| ATE223496T1 (de) | 2002-09-15 |
| TR199901164A3 (tr) | 1999-12-21 |
| EP0960945A3 (de) | 1999-12-22 |
| DE19823332A1 (de) | 1999-12-16 |
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