ES2183665A1 - Dna segments that are useful for selecting differentiated cell types and the applications thereof - Google Patents

Dna segments that are useful for selecting differentiated cell types and the applications thereof

Info

Publication number
ES2183665A1
ES2183665A1 ES009902251A ES9902251A ES2183665A1 ES 2183665 A1 ES2183665 A1 ES 2183665A1 ES 009902251 A ES009902251 A ES 009902251A ES 9902251 A ES9902251 A ES 9902251A ES 2183665 A1 ES2183665 A1 ES 2183665A1
Authority
ES
Spain
Prior art keywords
cell types
differentiated cell
applications
useful
dna segments
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
ES009902251A
Other languages
Spanish (es)
Other versions
ES2183665B1 (en
Inventor
Abril Alfonso Valera
Belda Josefa Maria Mayol
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universidad Miguel Hernandez de Elche UMH
Original Assignee
Universidad Miguel Hernandez de Elche UMH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universidad Miguel Hernandez de Elche UMH filed Critical Universidad Miguel Hernandez de Elche UMH
Priority to ES9902251A priority Critical patent/ES2183665B1/en
Priority to ES200102125A priority patent/ES2208024B1/en
Priority to PCT/ES2000/000391 priority patent/WO2001027266A1/en
Priority to AU77913/00A priority patent/AU7791300A/en
Publication of ES2183665A1 publication Critical patent/ES2183665A1/en
Application granted granted Critical
Publication of ES2183665B1 publication Critical patent/ES2183665B1/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Segmentos de ADN útiles para seleccionar tipos celulares diferenciados y sus aplicaciones. El segmento de ADN comprende (i) las regiones que contribuyen a formar y estabilizar el complejo de factores de transcripción de un gen específico de un tipo celular que es expresado específicamente en un tipo celular diferenciado, y (ii) un gen de selección funcionalmente conectado, de manera que se exprese cuando el segmento de ADN sea reconocido y transcrito por el complejo de factores de transcripción del gen específico. El segmento de ADN puede utilizarse en la obtención y selección in vitro de tipos celulares diferenciados a partir de unas células precursoras. Con los tipos celulares obtenidos se pueden generar bancos celulares y tisulares con fines terapéuticos o de investigación.DNA segments useful for selecting differentiated cell types and their applications. The DNA segment comprises (i) the regions that contribute to forming and stabilizing the transcription factor complex of a cell type-specific gene that is specifically expressed in a differentiated cell type, and (ii) a functionally linked selection gene. , so that it is expressed when the DNA segment is recognized and transcribed by the complex of transcription factors of the specific gene. The DNA segment can be used in obtaining and in vitro selection of differentiated cell types from precursor cells. With the cell types obtained, cell and tissue banks can be generated for therapeutic or research purposes.

ES9902251A 1999-10-14 1999-10-14 USEFUL DNA SEGMENTS TO SELECT DIFFERENTIATED CELLULAR TYPES AND THEIR APPLICATIONS. Expired - Fee Related ES2183665B1 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
ES9902251A ES2183665B1 (en) 1999-10-14 1999-10-14 USEFUL DNA SEGMENTS TO SELECT DIFFERENTIATED CELLULAR TYPES AND THEIR APPLICATIONS.
ES200102125A ES2208024B1 (en) 1999-10-14 1999-10-14 A METHOD FOR OBTAINING IN VITRO MOTHER CELLS OF INDIVIDUAL MAMMALS ADULTS.
PCT/ES2000/000391 WO2001027266A1 (en) 1999-10-14 2000-10-13 Dna segments that are useful for selecting differentiated cell types and the applications thereof
AU77913/00A AU7791300A (en) 1999-10-14 2000-10-13 Dna segments that are useful for selecting differentiated cell types and the applications thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES9902251A ES2183665B1 (en) 1999-10-14 1999-10-14 USEFUL DNA SEGMENTS TO SELECT DIFFERENTIATED CELLULAR TYPES AND THEIR APPLICATIONS.

Publications (2)

Publication Number Publication Date
ES2183665A1 true ES2183665A1 (en) 2003-03-16
ES2183665B1 ES2183665B1 (en) 2004-08-01

Family

ID=8310234

Family Applications (2)

Application Number Title Priority Date Filing Date
ES9902251A Expired - Fee Related ES2183665B1 (en) 1999-10-14 1999-10-14 USEFUL DNA SEGMENTS TO SELECT DIFFERENTIATED CELLULAR TYPES AND THEIR APPLICATIONS.
ES200102125A Expired - Fee Related ES2208024B1 (en) 1999-10-14 1999-10-14 A METHOD FOR OBTAINING IN VITRO MOTHER CELLS OF INDIVIDUAL MAMMALS ADULTS.

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES200102125A Expired - Fee Related ES2208024B1 (en) 1999-10-14 1999-10-14 A METHOD FOR OBTAINING IN VITRO MOTHER CELLS OF INDIVIDUAL MAMMALS ADULTS.

Country Status (3)

Country Link
AU (1) AU7791300A (en)
ES (2) ES2183665B1 (en)
WO (1) WO2001027266A1 (en)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994024274A1 (en) * 1993-04-21 1994-10-27 The University Of Edinburgh Isolation, selection and propagation of animal transgenic stem cells
WO1998035022A1 (en) * 1997-02-06 1998-08-13 Osiris Therapeutics, Inc. p21?CIP1 OR p27KIP1¿ EFFECTS ON THE REGULATION OF DIFFERENTIATION OF HUMAN MESENCHYMAL STEM CELLS
WO1998044142A1 (en) * 1997-04-01 1998-10-08 The General Hospital Corporation Molecular marker for muscle stem cells

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9401380D0 (en) * 1994-01-25 1994-03-23 Pharmaceutical Proteins Ltd Embryonic cell isolation
US5753506A (en) * 1996-05-23 1998-05-19 Cns Stem Cell Technology, Inc. Isolation propagation and directed differentiation of stem cells from embryonic and adult central nervous system of mammals
AU729377B2 (en) * 1997-10-23 2001-02-01 Asterias Biotherapeutics, Inc. Methods and materials for the growth of primate-derived primordial stem cells in feeder-free culture

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994024274A1 (en) * 1993-04-21 1994-10-27 The University Of Edinburgh Isolation, selection and propagation of animal transgenic stem cells
WO1998035022A1 (en) * 1997-02-06 1998-08-13 Osiris Therapeutics, Inc. p21?CIP1 OR p27KIP1¿ EFFECTS ON THE REGULATION OF DIFFERENTIATION OF HUMAN MESENCHYMAL STEM CELLS
WO1998044142A1 (en) * 1997-04-01 1998-10-08 The General Hospital Corporation Molecular marker for muscle stem cells

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
HERRERA, P.L. et al. "Two transgenic approaches to define the cell lineages in endocrine pancreas development", MOLECULAR AND CELULAR ENDOCRINOLOGY, 1998, Vol. 140, pßginas 45-50. Todo el documento. *
JUN-HSIANG, L. et al. "A tissue-specific promoter that can drive a foreign gene to express in the suprabasal urothelial cells of transgenic mice", PROC. NATL. ACAD. SCI. USA, 1995, Vol. 92, pßginas 679-683. Todo el documento. *

Also Published As

Publication number Publication date
ES2183665B1 (en) 2004-08-01
ES2208024A1 (en) 2004-06-01
ES2208024B1 (en) 2005-10-01
WO2001027266A1 (en) 2001-04-19
AU7791300A (en) 2001-04-23

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