ES2184661T1 - Procedimiento y aparato para recuperar plasmidos por ultrafiltracion. - Google Patents

Procedimiento y aparato para recuperar plasmidos por ultrafiltracion.

Info

Publication number
ES2184661T1
ES2184661T1 ES01922922T ES01922922T ES2184661T1 ES 2184661 T1 ES2184661 T1 ES 2184661T1 ES 01922922 T ES01922922 T ES 01922922T ES 01922922 T ES01922922 T ES 01922922T ES 2184661 T1 ES2184661 T1 ES 2184661T1
Authority
ES
Spain
Prior art keywords
plasmids
ultrafiltration
dna
appliance
procedure
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
ES01922922T
Other languages
English (en)
Other versions
ES2184661T3 (es
Inventor
Jack Thacher Leonard
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
EMD Millipore Corp
Original Assignee
Millipore Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=22727950&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ES2184661(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Millipore Corp filed Critical Millipore Corp
Publication of ES2184661T1 publication Critical patent/ES2184661T1/es
Application granted granted Critical
Publication of ES2184661T3 publication Critical patent/ES2184661T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
    • B01D61/14—Ultrafiltration; Microfiltration
    • B01D61/18—Apparatus therefor
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
    • B01D61/14—Ultrafiltration; Microfiltration
    • B01D61/149—Multistep processes comprising different kinds of membrane processes selected from ultrafiltration or microfiltration
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
    • B01D61/14—Ultrafiltration; Microfiltration
    • B01D61/16—Feed pretreatment
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2311/00—Details relating to membrane separation process operations and control
    • B01D2311/04—Specific process operations in the feed stream; Feed pretreatment
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

Landscapes

  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Water Supply & Treatment (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Saccharide Compounds (AREA)

Abstract

Un procedimiento para recuperar plásmidos u otro DNA que comprende las etapas de: (a) romper las paredes celulares para liberar los componentes celulares incluyendo plásmidos u otro DNA; (b) filtrar los componentes celulares a través de una o más membranas seleccionadas del grupo que consiste en microfiltración, filtración en bruto y sus combinaciones y recoger el filtrado; (c) filtrar el filtrado de (b) a través de una o más membranas de ultrafiltración de manera que se dejan los plásmidos u otro DNA como material retenido en la superficie superior de una o más membranas de ultrafiltración y (d) recuperar los plásmidos u otro DNA de la superficie superior de la membrana de ultrafiltración.
ES01922922T 2000-04-13 2001-03-29 Metodo y aparato para la recuperacion de plasmidos que implica ultrafiltracion. Expired - Lifetime ES2184661T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US19707800P 2000-04-13 2000-04-13
US197078P 2000-04-13

Publications (2)

Publication Number Publication Date
ES2184661T1 true ES2184661T1 (es) 2003-04-16
ES2184661T3 ES2184661T3 (es) 2005-09-01

Family

ID=22727950

Family Applications (1)

Application Number Title Priority Date Filing Date
ES01922922T Expired - Lifetime ES2184661T3 (es) 2000-04-13 2001-03-29 Metodo y aparato para la recuperacion de plasmidos que implica ultrafiltracion.

Country Status (6)

Country Link
US (2) US6759233B2 (es)
EP (1) EP1272627B1 (es)
JP (1) JP4150187B2 (es)
DE (2) DE60108950T2 (es)
ES (1) ES2184661T3 (es)
WO (1) WO2001079486A2 (es)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1290158B1 (en) * 2000-06-02 2006-12-06 Pall Corporation Processing of plasmid-containing fluids
US7074333B2 (en) 2001-01-19 2006-07-11 Millipore Corporation Recovery of linear nucleic acids by salt dilution and/or reduced pressure prior to continuous pressure differential ultrafiltration
US20030202909A1 (en) * 2002-04-29 2003-10-30 Genetix Limited Vacuum manifold and uses thereof
DE10238630A1 (de) * 2002-08-19 2004-03-04 Macherey, Nagel Gmbh & Co. Handelsgesellschaft Verfahren zur Isolierung biologischer Makromoleküle sowie Vorrichtung zur Durchführung dieses Verfahrens
US20040214180A1 (en) * 2003-04-28 2004-10-28 Hironori Kobayashi Method and kit for determining quantity of protein
JP4076978B2 (ja) * 2003-11-20 2008-04-16 ミリポア・コーポレイション プラスミドｄｎａ清浄化
US7700369B2 (en) 2004-07-27 2010-04-20 Protedyne Corporation Method and apparatus for applying a pressure differential to a multi-well plate
US7309589B2 (en) * 2004-08-20 2007-12-18 Vironix Llc Sensitive detection of bacteria by improved nested polymerase chain reaction targeting the 16S ribosomal RNA gene and identification of bacterial species by amplicon sequencing
US7510654B2 (en) * 2005-12-29 2009-03-31 Spf Innovations, Llc Method and apparatus for the filtration of biological samples
US8747669B1 (en) 2005-12-29 2014-06-10 Spf Innovations, Llc Method and apparatus for the filtration of biological samples
WO2011156734A2 (en) 2010-06-11 2011-12-15 Hitachi Chemical Co., Ltd. Method of characterizing vascular diseases
US10883100B2 (en) 2011-03-29 2021-01-05 Phynexus, Inc Devices and methods for plasmid purification
US11274292B2 (en) 2011-03-29 2022-03-15 Phynexus, Inc. Devices and methods for plasmid purification
US10597652B2 (en) * 2011-03-29 2020-03-24 Phynexus, Inc. Methods and devices for nucleic acid purification
JP5823031B2 (ja) * 2011-06-10 2015-11-25 日立化成株式会社 小胞捕捉デバイスおよびそれを用いるための方法
JP6297676B2 (ja) 2013-05-06 2018-03-20 日立化成株式会社 標的分子を捕捉するためのデバイス及び方法
US10266895B2 (en) 2014-11-05 2019-04-23 Hitachi Chemical Company Ltd. Exosomes and microvesicles in intestinal luminal fluids and stool and use of same for the assessment of inflammatory bowel disease
US10370719B2 (en) 2014-11-12 2019-08-06 Hitachi Chemical Co., Ltd. Method and device for diagnosing organ injury
JP6624704B2 (ja) 2015-08-31 2019-12-25 日立化成株式会社 尿路上皮疾患の評価のための分子法
MX2019007316A (es) * 2016-12-22 2019-09-27 Unibio As Eliminacion de acidos nucleicos y fragmentos de los mismos de un material de biomasa.
EP3774835A4 (en) * 2018-04-13 2022-01-19 National Agricultural Genotyping Center Universal lactic acid bacteria quantification kits, methods, compositions and apparatuses therefor
WO2019239514A1 (ja) * 2018-06-13 2019-12-19 日立化成株式会社 細胞溶解装置および細胞溶解方法
EP4127163A1 (en) 2020-03-31 2023-02-08 Richter-Helm Biologics GmbH & Co. KG Methods for producing plasmid dna
CN112940915B (zh) * 2021-03-31 2025-07-04 四川工易生物科技有限公司 一种质粒提取装置及方法
WO2024013278A1 (en) * 2022-07-13 2024-01-18 Dna Script Method of purifying solution comprising polynucleotide
WO2025157970A1 (en) 2024-01-26 2025-07-31 Richter Biologics Gmbh & Co. Kg Large scale methods for producing plasmid dna
CN118767685B (zh) * 2024-09-06 2025-02-07 济南绿德地生物科技有限公司 一种微生物菌剂生产的分离装置及方法

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4734192A (en) 1982-07-01 1988-03-29 Millipore Corporation Multiwell membrane filtration apparatus
US4948564A (en) 1986-10-28 1990-08-14 Costar Corporation Multi-well filter strip and composite assemblies
US5108704A (en) 1988-09-16 1992-04-28 W. R. Grace & Co.-Conn. Microfiltration apparatus with radially spaced nozzles
WO1992013963A1 (en) 1991-01-30 1992-08-20 Hyman Edward D Method for preparation of closed circular dna
US5116496A (en) 1991-03-19 1992-05-26 Minnesota Mining And Manufacturing Company Membrane-containing wells for microtitration and microfiltration
DE4143639C2 (de) * 1991-12-02 2002-10-24 Qiagen Gmbh Verfahren zur Isolierung und Reinigung von Nukleinsäuren
US5561064A (en) * 1994-02-01 1996-10-01 Vical Incorporated Production of pharmaceutical-grade plasmid DNA
CA2192262C (en) 1995-12-08 2011-03-15 Yoshihide Hayashizaki Method for purification and transfer to separation/detection systems of dna sequencing samples and plates used therefor
US6011148A (en) * 1996-08-01 2000-01-04 Megabios Corporation Methods for purifying nucleic acids
US5665247A (en) 1996-09-16 1997-09-09 Whatman Inc. Process for sealing microplates utilizing a thin polymeric film
EP1025263A2 (en) * 1997-10-23 2000-08-09 GUILLET, James Edwin Labelling of polymers and sequencing of nucleic acids
WO2000066723A1 (en) * 1999-05-04 2000-11-09 Millipore Corporation Method of ultrafiltration

Also Published As

Publication number Publication date
WO2001079486A3 (en) 2002-06-13
US7297534B2 (en) 2007-11-20
EP1272627B1 (en) 2005-02-16
DE60108950D1 (de) 2005-03-24
US6759233B2 (en) 2004-07-06
ES2184661T3 (es) 2005-09-01
EP1272627A2 (en) 2003-01-08
DE60108950T2 (de) 2006-02-23
US20030203453A1 (en) 2003-10-30
JP4150187B2 (ja) 2008-09-17
WO2001079486A2 (en) 2001-10-25
US20020001836A1 (en) 2002-01-03
DE1272627T1 (de) 2003-05-28
JP2003530854A (ja) 2003-10-21

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