ES2188384B1 - PROCEDURE FOR OBTAINING HUMAN DNA FROM GINGIVAL CREVICULAR FLUID. - Google Patents

PROCEDURE FOR OBTAINING HUMAN DNA FROM GINGIVAL CREVICULAR FLUID.

Info

Publication number
ES2188384B1
ES2188384B1 ES200101441A ES200101441A ES2188384B1 ES 2188384 B1 ES2188384 B1 ES 2188384B1 ES 200101441 A ES200101441 A ES 200101441A ES 200101441 A ES200101441 A ES 200101441A ES 2188384 B1 ES2188384 B1 ES 2188384B1
Authority
ES
Spain
Prior art keywords
human dna
crevicular fluid
gingival crevicular
procedure
obtaining
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
ES200101441A
Other languages
Spanish (es)
Other versions
ES2188384A1 (en
Inventor
Juan Manuel Morillo Velazquez
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Clinica Perio S L
Clinica Perio Sl
Original Assignee
Clinica Perio S L
Clinica Perio Sl
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Clinica Perio S L, Clinica Perio Sl filed Critical Clinica Perio S L
Priority to ES200101441A priority Critical patent/ES2188384B1/en
Publication of ES2188384A1 publication Critical patent/ES2188384A1/en
Application granted granted Critical
Publication of ES2188384B1 publication Critical patent/ES2188384B1/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D21/00—Separation of suspended solid particles from liquids by sedimentation
    • B01D21/26—Separation of sediment aided by centrifugal force or centripetal force
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Physics & Mathematics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Urology & Nephrology (AREA)
  • Plant Pathology (AREA)
  • Hematology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Procedimiento de obtención de ADN humano a partir de fluido crevicular gingival. Realiza la extracción de ADN humano, para su análisis mediante detección molecular, así como para el análisis de la susceptibilidad genética a cualquier enfermedad, sin afectar a la actividad de la Taq-polimerasa en la PCR, obteniéndose un producto de correcta visualización tras su electroforesis en gel de agarosa y mediante tinción con bromuro de etidio, así como sometible a digestión con enzimas de restricción (RFLP) para estudio alélico o a su secuenciación directa, con calidad diagnóstica, en base a realizar una cuidadosa obtención del fluido crevicular gingival mediante puntas o tiras de papel absorbente o un cepillo interdental, evitando la contaminación de la muestra con las propias células del operador.Procedure for obtaining human DNA from gingival crevicular fluid. It carries out the extraction of human DNA, for its analysis by molecular detection, as well as for the analysis of the genetic susceptibility to any disease, without affecting the activity of Taq polymerase in the PCR, obtaining a product of correct visualization after its electrophoresis in agarose gel and by staining with ethidium bromide, as well as subject to digestion with restriction enzymes (RFLP) for allelic study or to its direct sequencing, with diagnostic quality, based on careful preparation of gingival crevicular fluid by means of tips or strips of absorbent paper or an interdental brush, preventing contamination of the sample with the operator's own cells.

ES200101441A 2001-06-21 2001-06-21 PROCEDURE FOR OBTAINING HUMAN DNA FROM GINGIVAL CREVICULAR FLUID. Expired - Fee Related ES2188384B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES200101441A ES2188384B1 (en) 2001-06-21 2001-06-21 PROCEDURE FOR OBTAINING HUMAN DNA FROM GINGIVAL CREVICULAR FLUID.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES200101441A ES2188384B1 (en) 2001-06-21 2001-06-21 PROCEDURE FOR OBTAINING HUMAN DNA FROM GINGIVAL CREVICULAR FLUID.

Publications (2)

Publication Number Publication Date
ES2188384A1 ES2188384A1 (en) 2003-06-16
ES2188384B1 true ES2188384B1 (en) 2005-01-01

Family

ID=8498138

Family Applications (1)

Application Number Title Priority Date Filing Date
ES200101441A Expired - Fee Related ES2188384B1 (en) 2001-06-21 2001-06-21 PROCEDURE FOR OBTAINING HUMAN DNA FROM GINGIVAL CREVICULAR FLUID.

Country Status (1)

Country Link
ES (1) ES2188384B1 (en)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990004787A1 (en) * 1988-10-26 1990-05-03 The Board Of Trustees Of The University Of Illinois Method for determination of the type and severity of periodontal disease states
WO1998044158A1 (en) * 1997-03-28 1998-10-08 Epitope, Inc. Simultaneous collection of dna and non-nucleic analytes from oral fluids
ES2128265A1 (en) * 1997-06-04 1999-05-01 Euroespes S L Genetic kit for the molecular identification of Alzheimer's disease
WO2000050564A2 (en) * 1999-02-26 2000-08-31 Myriad Genetics, Inc. Method for extracting dna from dried specimens

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990004787A1 (en) * 1988-10-26 1990-05-03 The Board Of Trustees Of The University Of Illinois Method for determination of the type and severity of periodontal disease states
WO1998044158A1 (en) * 1997-03-28 1998-10-08 Epitope, Inc. Simultaneous collection of dna and non-nucleic analytes from oral fluids
ES2128265A1 (en) * 1997-06-04 1999-05-01 Euroespes S L Genetic kit for the molecular identification of Alzheimer's disease
WO2000050564A2 (en) * 1999-02-26 2000-08-31 Myriad Genetics, Inc. Method for extracting dna from dried specimens

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
HARTY LC et al. Collection of buccal cell DNA using treated cards. Cancer Epidemiol. Biomarkers Prev., Mayo 2000, Vol. 9, Núm. 5, páginas 501-506. Páginas 501,502. *

Also Published As

Publication number Publication date
ES2188384A1 (en) 2003-06-16

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