ES2189783T3 - Celulas huesped de levaduras transformadas de forma estable y su utilizacion parala produccion de albumina. - Google Patents

Celulas huesped de levaduras transformadas de forma estable y su utilizacion parala produccion de albumina.

Info

Publication number
ES2189783T3
ES2189783T3 ES90109683T ES90109683T ES2189783T3 ES 2189783 T3 ES2189783 T3 ES 2189783T3 ES 90109683 T ES90109683 T ES 90109683T ES 90109683 T ES90109683 T ES 90109683T ES 2189783 T3 ES2189783 T3 ES 2189783T3
Authority
ES
Spain
Prior art keywords
lifting
sequence
yeast
albumin
anfritrion
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES90109683T
Other languages
English (en)
Inventor
Ken Okabayashi
Hideyuki Oi
Kazumoto Hirabayashi
Masami Miura
Miho Shimizu
Haruhide Kawabe
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Tanabe Pharma Corp
Original Assignee
Mitsubishi Pharma Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP1234481A external-priority patent/JPH0669365B2/ja
Application filed by Mitsubishi Pharma Corp filed Critical Mitsubishi Pharma Corp
Application granted granted Critical
Publication of ES2189783T3 publication Critical patent/ES2189783T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/76—Albumins
    • C07K14/765—Serum albumin, e.g. HSA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Mycology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

SE PRESENTA UN PLASMIDO PARA UN ANFRITRION DE LEVADURA, COMPRENDIENDO EL MISMO, EN SECUENCIA: (1) UN PROMOTOR DERIVADO DE UNA LEVADURA, (2) UNA ZONA CODIFICANTE DE ALBUMINA SITUADA BAJO EL CONTROL DEL PROMOTOR DERIVADO DE LA LEVADURA, (3) UN FINALIZADOR DE TRANSCRIPCION Y (4) UNA SECUENCIA HOMOLOGA A UNA PARTE DE LA SECUENCIA CROMOSOMICA DEL ANFRITRION DE LEVADURA DE TAL FORMA QUE EL PLASMIDO SEA CAPAZ DE INTEGRARSE DENTRO DEL CROMOSOMA DE LA CELULA ANFRITRIONA DE LEVADURA Y EN LA CUAL EL PLASMIDO SEA CAPAZ DE DUPLICACION AUTONOMA EN CELULAS DE LEVADURA; TAMBIEN SE PRESENTA UN ANFRITRION DE LEVADURA TRANSFORMADO POR EL PLASMIDO DESCRITO; UN METODO PARA PRODUCIR UN TRANSFORMANTE DE LEVADURA QUE COMPRENDE LA TRANSFORMACION DE UN ANFITRION DE LEVADURA MEDIANTE LA INTEGRACION DE AL MENOS DOS PLASMIDOS DIFERENTES CADA UNO DE LOS CUALES CONTENIENDO UNA SECUENCIA HOMONIMA A UNA PARTE DE LA SECUENCIA CROMOSOMICA DE LA LEVADURA ANFITRIONA, UNO CADA VEZ, EN EL CROMOSOMA DE LA CELULA ANFITRIONA DELEVADURA. ASI MISMO SE PRESENTA UN METODO PARA PRODUCIR ALBUMINA QUE COMPRENDE EL CULTIVO DEL TRANSFORMANTE MENCIONADO Y LA RECUPERACION DE LA ALBUMINA ASI PRODUCIDA.
ES90109683T 1989-05-22 1990-05-22 Celulas huesped de levaduras transformadas de forma estable y su utilizacion parala produccion de albumina. Expired - Lifetime ES2189783T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP12992789 1989-05-22
JP1234481A JPH0669365B2 (ja) 1989-05-22 1989-09-08 アルブミン遺伝子を含むプラスミド、形質転換体、形質転換体の製造方法、アルブミンの製造方法

Publications (1)

Publication Number Publication Date
ES2189783T3 true ES2189783T3 (es) 2003-07-16

Family

ID=26465186

Family Applications (1)

Application Number Title Priority Date Filing Date
ES90109683T Expired - Lifetime ES2189783T3 (es) 1989-05-22 1990-05-22 Celulas huesped de levaduras transformadas de forma estable y su utilizacion parala produccion de albumina.

Country Status (5)

Country Link
EP (1) EP0399455B1 (es)
AU (1) AU640654B2 (es)
CA (1) CA2017176A1 (es)
DE (1) DE69034051T2 (es)
ES (1) ES2189783T3 (es)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2649991B2 (fr) * 1988-08-05 1994-03-04 Rhone Poulenc Sante Utilisation de derives stables du plasmide pkd1 pour l'expression et la secretion de proteines heterologues dans les levures du genre kluyveromyces
JP3230091B2 (ja) * 1990-06-25 2001-11-19 ウェルファイド株式会社 ヒト血清アルブミンの着色抑制方法
JPH0671432B2 (ja) * 1991-03-20 1994-09-14 株式会社ミドリ十字 ヒト血清アルブミンの製造方法
FR2679249B1 (fr) * 1991-07-15 1993-11-26 Centre Nal Recherc Scientifique Souches de levure avec integration stable de genes heterologues.
JPH07308199A (ja) 1994-05-18 1995-11-28 Green Cross Corp:The ヒト血清アルブミンの製造方法
DE10036491A1 (de) 2000-07-25 2002-02-07 Roche Diagnostics Gmbh Expression von Alkalischer Phosphatase in Hefe
GB0217033D0 (en) 2002-07-23 2002-08-28 Delta Biotechnology Ltd Gene and polypeptide sequences
RU2337966C2 (ru) * 2006-08-07 2008-11-10 Государственное учреждение Гематологический научный центр (ГНЦ) РАМН Препарат рекомбинантного человеческого сывороточного альбумина и способ его получения
RU2010151453A (ru) 2008-05-15 2012-06-20 Р-Тек Уено, Лтд. (Jp) Фармацевтическая композиция для лечения сухости глаз и/или повреждения конъюнктивы и роговицы
JP7146637B2 (ja) 2015-12-22 2022-10-04 アルブミディクス リミティド 改善されたタンパク質発現株
US11130979B2 (en) 2017-06-20 2021-09-28 Albumedix Ltd Protein expression strains

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2563533B1 (fr) * 1984-04-27 1986-08-22 Centre Nat Rech Scient Procede d'amplification de l'expression d'un gene determine chez bacillus subtilis et souches obtenues
US4882279A (en) * 1985-10-25 1989-11-21 Phillips Petroleum Company Site selective genomic modification of yeast of the genus pichia
GB8613388D0 (en) * 1986-06-03 1986-07-09 Delta Biotechnology Ltd Induction of galactose regulated gene expression in yeast
AR247761A1 (es) * 1987-07-28 1995-03-31 Gist Brocades Nv Metodo para producir un polipeptido en una celula hospedante de kluyveromyces, dicha celula hospedante transformada y secuencia a adn empleada
SE459586B (sv) * 1987-09-14 1989-07-17 Mta Szegedi Biolog Koezponti Strukturgen som kodar foer autentiskt humant serum albumin och foerfarande foer dess framstaellning
FR2649991B2 (fr) * 1988-08-05 1994-03-04 Rhone Poulenc Sante Utilisation de derives stables du plasmide pkd1 pour l'expression et la secretion de proteines heterologues dans les levures du genre kluyveromyces

Also Published As

Publication number Publication date
EP0399455A2 (en) 1990-11-28
CA2017176A1 (en) 1990-11-22
EP0399455A3 (en) 1991-06-19
AU5578690A (en) 1990-11-22
DE69034051T2 (de) 2003-11-27
AU640654B2 (en) 1993-09-02
EP0399455B1 (en) 2003-03-26
DE69034051D1 (de) 2003-04-30

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