ES2189869T3 - Procedimiento para el enriquecimiento de poblaciones de celulas raras. - Google Patents

Procedimiento para el enriquecimiento de poblaciones de celulas raras.

Info

Publication number
ES2189869T3
ES2189869T3 ES96910382T ES96910382T ES2189869T3 ES 2189869 T3 ES2189869 T3 ES 2189869T3 ES 96910382 T ES96910382 T ES 96910382T ES 96910382 T ES96910382 T ES 96910382T ES 2189869 T3 ES2189869 T3 ES 2189869T3
Authority
ES
Spain
Prior art keywords
erythrocytes
blood sample
fetal nucleated
enrichment
procedure
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES96910382T
Other languages
English (en)
Inventor
David W Sammons
Michael Manley
Joseph G Utermohlen
Garland E Twitty
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bioseparations Inc
Original Assignee
Bioseparations Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/401,131 external-priority patent/US5676849A/en
Application filed by Bioseparations Inc filed Critical Bioseparations Inc
Application granted granted Critical
Publication of ES2189869T3 publication Critical patent/ES2189869T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/49—Blood
    • G01N33/491—Blood by separating the blood components
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D17/00—Separation of liquids, not provided for elsewhere, e.g. by thermal diffusion
    • B01D17/02—Separation of non-miscible liquids
    • B01D17/0202—Separation of non-miscible liquids by ab- or adsorption
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D17/00—Separation of liquids, not provided for elsewhere, e.g. by thermal diffusion
    • B01D17/02—Separation of non-miscible liquids
    • B01D17/0217—Separation of non-miscible liquids by centrifugal force
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D17/00—Separation of liquids, not provided for elsewhere, e.g. by thermal diffusion
    • B01D17/06—Separation of liquids from each other by electricity
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D17/00—Separation of liquids, not provided for elsewhere, e.g. by thermal diffusion
    • B01D17/08—Thickening liquid suspensions by filtration
    • B01D17/085—Thickening liquid suspensions by filtration with membranes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D21/00—Separation of suspended solid particles from liquids by sedimentation
    • B01D21/26—Separation of sediment aided by centrifugal force or centripetal force
    • B01D21/262—Separation of sediment aided by centrifugal force or centripetal force by using a centrifuge
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D17/00—Separation of liquids, not provided for elsewhere, e.g. by thermal diffusion
    • B01D17/02—Separation of non-miscible liquids
    • B01D17/04—Breaking emulsions
    • B01D17/047—Breaking emulsions with separation aids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2221/00—Applications of separation devices
    • B01D2221/10—Separation devices for use in medical, pharmaceutical or laboratory applications, e.g. separating amalgam from dental treatment residues

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Thermal Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Pathology (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Biophysics (AREA)
  • Electrochemistry (AREA)
  • Ecology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

SE PRESENTA UN METODO PARA ENRIQUECER UNA MUESTRA DE SANGRE TOTAL CON CELULAS ESCASAS. UNA MUESTRA DE SANGRE (12) SE DISPONE EN CAPAS EN FICOLL/HYPAQUE-1119 (14) Y SE CENTRIFUGA PARA ELIMINAR LOS ERITROCITOS MATERNOS (16) Y EL RESTO DE LA MUESTRA DE SANGRE CENTRIFUGADA SE HACE PASAR A TRAVES DE UN APARATO SEPARADOR ESTABILIZADO DE CARGA-FLUJO A CONTRACORRIENTE CONTROLADO POR LA GRAVEDAD (20) PARA SEPARAR LOS ERITROCITOS NUCLEADOS FETALES (26) DE LOS DEMAS COMPONENTES CELULARES MEDIANTE LA APLICACION DE UN CAMPO ELECTRICO. LOS ERITROCITOS NUCLEADOS FETALES SEPARADOS (26) SON SOMETIDOS A HEMOLISIS CON AMONIACO (30) PARA ELIMINAR LOS ERITROCITOS MADUROS Y RECUPERAR LOS ERITROCITOS NUCLEADOS FETALES ENRIQUECIDOS.
ES96910382T 1995-03-08 1996-03-07 Procedimiento para el enriquecimiento de poblaciones de celulas raras. Expired - Lifetime ES2189869T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/401,131 US5676849A (en) 1994-10-21 1995-03-08 Method for enrichment of fetal cell population from maternal whole blood samples
PCT/US1995/013631 WO1996012945A1 (en) 1994-10-21 1995-10-20 Method for separating nucleated fetal erythrocytes

Publications (1)

Publication Number Publication Date
ES2189869T3 true ES2189869T3 (es) 2003-07-16

Family

ID=23586432

Family Applications (1)

Application Number Title Priority Date Filing Date
ES96910382T Expired - Lifetime ES2189869T3 (es) 1995-03-08 1996-03-07 Procedimiento para el enriquecimiento de poblaciones de celulas raras.

Country Status (6)

Country Link
EP (1) EP0813442B1 (es)
JP (1) JPH11502106A (es)
AT (1) ATE228026T1 (es)
DE (1) DE69624908T2 (es)
ES (1) ES2189869T3 (es)
WO (1) WO1996027420A1 (es)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2813891B1 (fr) * 2000-09-14 2005-01-14 Immunotech Sa Reactif multifonctionnel pour erythrocytes mettant en jeu des carbamates et applications
US8986944B2 (en) 2001-10-11 2015-03-24 Aviva Biosciences Corporation Methods and compositions for separating rare cells from fluid samples
US8980568B2 (en) 2001-10-11 2015-03-17 Aviva Biosciences Corporation Methods and compositions for detecting non-hematopoietic cells from a blood sample
DE60328193D1 (de) * 2003-10-16 2009-08-13 Sequenom Inc Nicht invasiver Nachweis fötaler genetischer Merkmale
WO2006119569A1 (en) * 2005-05-11 2006-11-16 Genetic Technologies Limtied Methods of enriching fetal cells
CN101583722A (zh) * 2006-07-14 2009-11-18 阿维瓦生物科学股份有限公司 从生物学样品检测稀有细胞的方法和组合物
JP5265815B2 (ja) * 2010-08-20 2013-08-14 国立大学法人北陸先端科学技術大学院大学 血球の密度変化を利用した密度勾配遠心による有核赤血球の回収法
ITRM20120028A1 (it) * 2012-01-25 2013-07-26 Natalia Malara Metodo per isolare cellule tumorali circolanti.

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4925572A (en) * 1987-10-20 1990-05-15 Pall Corporation Device and method for depletion of the leukocyte content of blood and blood components
US5192553A (en) * 1987-11-12 1993-03-09 Biocyte Corporation Isolation and preservation of fetal and neonatal hematopoietic stem and progenitor cells of the blood and methods of therapeutic use
US5336387A (en) * 1990-09-11 1994-08-09 Bioseparations, Inc. Electrical separator apparatus and method of counterflow gradient focusing
US5439571A (en) * 1991-11-19 1995-08-08 Bioseparations, Inc. Apparatus and method for enhanced resolution continuous flow zone electrophoresis
US5457024A (en) * 1993-01-22 1995-10-10 Aprogenex, Inc. Isolation of fetal erythrocytes
US5489386A (en) * 1994-01-31 1996-02-06 Applied Imaging Density gradient medium for the separation of cells
US5432054A (en) * 1994-01-31 1995-07-11 Applied Imaging Method for separating rare cells from a population of cells

Also Published As

Publication number Publication date
ATE228026T1 (de) 2002-12-15
DE69624908D1 (de) 2003-01-02
JPH11502106A (ja) 1999-02-23
EP0813442A4 (en) 1998-09-09
WO1996027420A1 (en) 1996-09-12
DE69624908T2 (de) 2003-07-03
EP0813442A1 (en) 1997-12-29
EP0813442B1 (en) 2002-11-20

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