ES2191680T3 - Gen de aspartoquinasa variante. - Google Patents

Gen de aspartoquinasa variante.

Info

Publication number
ES2191680T3
ES2191680T3 ES94902116T ES94902116T ES2191680T3 ES 2191680 T3 ES2191680 T3 ES 2191680T3 ES 94902116 T ES94902116 T ES 94902116T ES 94902116 T ES94902116 T ES 94902116T ES 2191680 T3 ES2191680 T3 ES 2191680T3
Authority
ES
Spain
Prior art keywords
aspartokinase
corynebacterium
lysine
gen
various
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES94902116T
Other languages
English (en)
Inventor
Masakazu Sugimoto
Yuri Ogawa
Tomoto Suzuki
Akiko Tanaka
Hiroshi Matsui
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ajinomoto Co Inc
Original Assignee
Ajinomoto Co Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ajinomoto Co Inc filed Critical Ajinomoto Co Inc
Application granted granted Critical
Publication of ES2191680T3 publication Critical patent/ES2191680T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1217—Phosphotransferases with a carboxyl group as acceptor (2.7.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Distillation Of Fermentation Liquor, Processing Of Alcohols, Vinegar And Beer (AREA)
  • Ceramic Products (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)

Abstract

SE PRESENTA UN TRANSFORMANTE CON UNA VELOCIDAD INCREMENTADA EN LA PRODUCCION Y SECRECION DE L-LISINA QUE SE OBTIENE INTRODUCIENDO EN UNA BACTERIA UN DNA RECOMBINANTE QUE PUEDA SER REPLICADO EN UNA BACTERIA Y QUE CONTENGA UN FRAGMENTO DE DNA QUE SE ORIGINA EN UNA BACTERIA Y QUE CODIFICA UNA PROTEINA DE UNA {AL}-SUBUNIDAD DE ASPARTOQUINASA MEDIANTE LO CUAL SE LIBERA UNA INHIBICION DE REALIMENTACION SINERGISTICA MEDIANTE L-LISINA Y LTREONINA O UN FRAGMENTO DE DNA QUE SE ORIGINA EN UNA BACTERIA Y QUE CODIFICA UNA {BE}-SUBUNIDAD DE ASPARTOQUINASA MEDIANTE LO CUAL SE LIBERA UNA INHIBICION DE REALIMENTACION SINERGISTICA MEDIANTE L-LISINA Y L-TREONINA. EL TRANSFORMANTE OBTENIDO SE CULTIVA EN UN MEDIO ADECUADO Y SE SEPARA LA L-LISINA FORMADA.
ES94902116T 1993-04-27 1993-12-14 Gen de aspartoquinasa variante. Expired - Lifetime ES2191680T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP10145093A JP3473042B2 (ja) 1992-04-28 1993-04-27 変異型アスパルトキナーゼ遺伝子

Publications (1)

Publication Number Publication Date
ES2191680T3 true ES2191680T3 (es) 2003-09-16

Family

ID=14301034

Family Applications (1)

Application Number Title Priority Date Filing Date
ES94902116T Expired - Lifetime ES2191680T3 (es) 1993-04-27 1993-12-14 Gen de aspartoquinasa variante.

Country Status (8)

Country Link
US (1) US5688671A (es)
EP (1) EP0699759B1 (es)
JP (1) JP3473042B2 (es)
AT (1) ATE235555T1 (es)
DE (1) DE69332805T2 (es)
DK (1) DK0699759T3 (es)
ES (1) ES2191680T3 (es)
WO (1) WO1994025605A1 (es)

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US6696561B1 (en) 1909-07-09 2004-02-24 Basf Aktiengesellschaft Corynebacterium glutamicum genes encoding proteins involved in membrane synthesis and membrane transport
JP3473042B2 (ja) * 1992-04-28 2003-12-02 味の素株式会社 変異型アスパルトキナーゼ遺伝子
US5877179A (en) * 1992-09-29 1999-03-02 The United States Of America As Represented By The Department Of Health And Human Services Xanthines for identifying CFTR--binding compounds useful for activating chloride conductance in animal cells
CN1165619C (zh) * 1995-06-07 2004-09-08 味之素株式会社 制备l-赖氨酸的方法
JP4035855B2 (ja) 1996-06-05 2008-01-23 味の素株式会社 Ｌ−リジンの製造法
SK285201B6 (sk) * 1996-12-05 2006-08-03 Ajinomoto Co., Inc. Rekombinantná DNA, autonómne reprodukovaná v bunkách koryneformnej baktérie, koryneformná baktéria a spôsob výroby L-lyzínu, vektor pVK7
JP4075087B2 (ja) * 1996-12-05 2008-04-16 味の素株式会社 Ｌ−リジンの製造法
CA2336967C (en) 1998-07-10 2010-06-29 The United States Of America, Represented By The Secretary, Department Of Health And Human Services A3 adenosine receptor antagonists
EP1172437B1 (en) * 1999-04-19 2011-05-25 Kyowa Hakko Bio Co., Ltd. Novel desensitized aspartokinase
MXPA01013123A (es) 1999-06-25 2002-06-21 Basf Ag Genes de corynebacterium glutamicum que codifican proteinas de via metabolica.
US7270984B1 (en) 1999-06-25 2007-09-18 Basf Aktiengesellschaft Polynucleotides encoding a 6-phosphogluconolactonase polypeptide from corynebacterium glutamicum
EP1702980A1 (en) 1999-07-01 2006-09-20 Basf Aktiengesellschaft Corynebacterium glutamicum gene encoding Hpr of phosphoenolpyruvate:sugar phosphotransferase system
US6884614B1 (en) 1999-07-01 2005-04-26 Basf Aktiengesellschaft Corynebacterium glutamicum genes encoding phosphoenolpyruvate: sugar phosphotransferase system proteins
AU781091B2 (en) 1999-07-02 2005-05-05 Ajinomoto Co., Inc. DNA encoding sucrose PTS enzyme II
US6797509B1 (en) 1999-07-09 2004-09-28 Degussa-Huls Ag Nucleotide sequences which code for the tal gene
PL354310A1 (en) 1999-09-15 2004-01-12 Basf Plant Science Gmbh Plants having altered amino acid contents and method for the production thereof
JP2001120269A (ja) * 1999-10-22 2001-05-08 Ajinomoto Co Inc 発酵法によるｌ−リジンの製造法
CN1230525C (zh) * 1999-12-24 2005-12-07 味之素株式会社 生产l－氨基酸的方法和新型基因
US6927046B1 (en) 1999-12-30 2005-08-09 Archer-Daniels-Midland Company Increased lysine production by gene amplification using coryneform bacteria
US7087589B2 (en) 2000-01-14 2006-08-08 The United States Of America As Represented By The Department Of Health And Human Services Methanocarba cycloakyl nucleoside analogues
DE10210527A1 (de) 2002-03-09 2003-09-18 Degussa Allele des aceA-Gens aus coryneformen Bakterien
WO2007086546A1 (en) * 2006-01-26 2007-08-02 Ajinomoto Co., Inc. A method for producing an l-amino acid using a bacterium of enterobacteriaceae family with attenuated expression of the aldh gene
EP2004803A2 (en) 2006-03-23 2008-12-24 Ajinomoto Co., Inc. A method for producing an l-amino acid using bacterium of theenterobacteriaceae family with attenuated expression of a gene coding for small rna
EP2386650B1 (en) * 2006-04-07 2013-07-03 Evonik Degussa GmbH Method for producing L-amino acids using the gap promoter
EP2007873B1 (en) * 2006-04-18 2015-11-18 Ajinomoto Co., Inc. A METHOD FOR PRODUCING AN L-AMINO ACID USING A BACTERIUM OF THE ENTEROBACTERIACEAE FAMILY WITH ATTENUATED EXPRESSION OF THE sfmACDFH-fimZ CLUSTER OR THE fimZ GENE
JP2009165355A (ja) * 2006-04-28 2009-07-30 Ajinomoto Co Inc Ｌ−アミノ酸を生産する微生物及びｌ−アミノ酸の製造法
EP2035569A1 (en) * 2006-06-01 2009-03-18 Ajinomoto Co., Inc. A method for producing an l-amino acid using a bacterium of the enterobacteriaceae family with attenuated expression of the rcsa gene
RU2337961C2 (ru) * 2006-07-04 2008-11-10 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) СПОСОБ ПОЛУЧЕНИЯ L-ТРЕОНИНА С ИСПОЛЬЗОВАНИЕМ БАКТЕРИИ, ПРИНАДЛЕЖАЩЕЙ К РОДУ Escherichia, В КОТОРОЙ ИНАКТИВИРОВАН ОПЕРОН rspAB
EP1881076A1 (en) * 2006-07-17 2008-01-23 Evonik Degussa GmbH Method for producing L-amino acids
CN103215319A (zh) * 2006-07-19 2013-07-24 味之素株式会社 使用肠杆菌科细菌产生l-氨基酸的方法
JP2010017081A (ja) 2006-10-10 2010-01-28 Ajinomoto Co Inc Ｌ−アミノ酸の製造法
WO2008072761A2 (en) * 2006-12-11 2008-06-19 Ajinomoto Co., Inc. Method for producing an l-amino acid
RU2006143864A (ru) * 2006-12-12 2008-06-20 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) (RU) СПОСОБ ПОЛУЧЕНИЯ L-АМИНОКИСЛОТ С ИСПОЛЬЗОВАНИЕМ БАКТЕРИИ СЕМЕЙСТВА ENTEROBACTERIACEAE, В КОТОРОЙ ОСЛАБЛЕНА ЭКСПРЕССИЯ ГЕНОВ cynT, cynS, cynX, ИЛИ cynR, ИЛИ ИХ КОМБИНАЦИИ
RU2006145712A (ru) * 2006-12-22 2008-06-27 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) (RU) Способ получения l-аминокислот методом ферментации с использованием бактерий, обладающих повышенной способностью к утилизации глицерина
JP2010110217A (ja) * 2007-02-22 2010-05-20 Ajinomoto Co Inc Ｌ−アミノ酸生産菌及びｌ−アミノ酸の製造法
JP5217780B2 (ja) * 2008-02-08 2013-06-19 味の素株式会社 Ｌ−アミノ酸を生産する微生物及びｌ−アミノ酸の製造法
JP5445453B2 (ja) 2008-07-09 2014-03-19 味の素株式会社 アミノヒドロキシ安息香酸類の製造方法
JP2012029565A (ja) 2008-11-27 2012-02-16 Ajinomoto Co Inc Ｌ−アミノ酸の製造法
US8518957B2 (en) 2009-12-02 2013-08-27 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Methanocarba adenosine derivatives, pharmaceutical compositions, and method of reducing intraocular pressure
EP2374873A1 (en) 2010-04-08 2011-10-12 Technische Universität Hamburg-Harburg Modified aspartate kinase from corynebacterium and its application for amino acid production
WO2013179711A1 (ja) 2012-05-29 2013-12-05 味の素株式会社 ３－アセチルアミノ－４－ヒドロキシ安息香酸の製造方法
DE102012016716A1 (de) 2012-08-22 2014-02-27 Forschungszentrum Jülich GmbH Verfahren zur Herstellung von Vektoren enthaltend ein für in seiner feedback-Inhibierung gemindertes oder ausgeschaltetes Enzym kodierendes Gen und deren Verwendung für die Herstellung von Aminosäuren und Nukleotiden
DE102014208199A1 (de) 2014-04-30 2015-11-05 Evonik Degussa Gmbh Verfahren zur Produktion von L-Aminosäuren unter Verwendung eines alkaliphilen Bakteriums
PL2940143T3 (pl) 2014-04-30 2020-06-29 Evonik Operations Gmbh Sposób wytwarzania L-aminokwasów z użyciem bakterii bazofilowych
JP6415845B2 (ja) * 2014-04-30 2018-10-31 日本テクニカ株式会社 展示物の展示及び収容用ケースの構造
EP2940039A1 (de) 2014-04-30 2015-11-04 Evonik Degussa GmbH Verfahren zur Produktion von L-Aminosäuren in Corynebakterien unter Verwendung eines Glycin spaltenden Systems
EP2940144A1 (de) 2014-04-30 2015-11-04 Evonik Degussa GmbH Verfahren zur Produktion von L-Lysin unter Verwendung eines alkaliphilen Bakteriums
KR102011994B1 (ko) * 2017-06-30 2019-08-20 씨제이제일제당 주식회사 신규한 아스파토키나제 변이체 및 이를 이용한 l-아미노산의 제조방법
EP3456833A1 (en) 2017-09-18 2019-03-20 Evonik Degussa GmbH Method for the fermentative production of l-amino acids
EP3467099A1 (en) * 2017-10-05 2019-04-10 Evonik Degussa GmbH Method for the fermentative production of l-amino acids
EP3498853A1 (en) 2017-12-14 2019-06-19 Evonik Degussa GmbH Method for the fermentative production of l-lysine
EP3594355A1 (en) 2018-07-12 2020-01-15 Evonik Operations GmbH Method for the fermentative production of l-lysine
EP3599282B1 (en) 2018-07-24 2021-03-17 Evonik Operations GmbH Method for the fermentative production of l-lysine
RU2019128538A (ru) 2018-09-26 2021-03-11 Эвоник Оперейшенс ГмбХ Способ ферментативного получения l-лизина
EP3660158A1 (en) 2018-11-29 2020-06-03 Evonik Operations GmbH Method for the fermentative production of l-lysine
EP3670525A1 (en) 2018-12-18 2020-06-24 Evonik Operations GmbH Method for the fermentative production of l-lysine using c. glutamicum strains with a mutated kup transporter
US10829746B2 (en) 2019-01-23 2020-11-10 Evonik Operations Gmbh Method for the fermentative production of L-lysine
WO2021048353A1 (en) 2019-09-11 2021-03-18 Evonik Operations Gmbh Coryneform bacteria with a heterologous threonine transporter and their use in the production of l-threonine
WO2023041425A1 (en) 2021-09-20 2023-03-23 Evonik Operations Gmbh Method for the fermentative production of l-lysine using c. glutamicum strains expressing modified gluconate repressor proteins gntr1 and gntr2
EP4497818A1 (en) 2023-07-25 2025-01-29 Evonik Operations GmbH Method for the fermentative production of l-lysine using c. glutamicum strains expressing heterologous nicotinamide nucleotide transhydrogenase pntab
CN117327677B (zh) * 2023-09-26 2024-09-20 江南大学 天冬氨酸激酶突变体及其应用
EP4613854A1 (en) 2024-03-05 2025-09-10 Evonik Operations GmbH Method for the fermentative production of l-lysine using c. glutamicum strains expressing a heterologous nicotinamide nucleotide transhydrogenase pntab and having an increased activity of a myo-inositol permease

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JPS5763095A (en) * 1980-09-30 1982-04-16 Ajinomoto Co Inc Production of l-lysine through fermentation process
DE3908201A1 (de) * 1989-03-14 1990-09-27 Degussa Verfahren zur fermentativen herstellung von l-lysin
JP3473042B2 (ja) * 1992-04-28 2003-12-02 味の素株式会社 変異型アスパルトキナーゼ遺伝子
JPH06261766A (ja) * 1993-03-16 1994-09-20 Mitsubishi Petrochem Co Ltd フィードバックインヒビションの解除されたアスパルトキナーゼをコードする遺伝子ｄｎａ及びその利用

Also Published As

Publication number Publication date
EP0699759B1 (en) 2003-03-26
US5688671A (en) 1997-11-18
WO1994025605A1 (fr) 1994-11-10
JPH0662866A (ja) 1994-03-08
EP0699759A1 (en) 1996-03-06
DE69332805T2 (de) 2004-02-19
JP3473042B2 (ja) 2003-12-02
DK0699759T3 (da) 2003-09-15
EP0699759A4 (en) 1999-09-22
DE69332805D1 (de) 2003-04-30
ATE235555T1 (de) 2003-04-15

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