ES2192945A1 - Un metodo para interferir con la infeccion de virus en plantas. - Google Patents

Un metodo para interferir con la infeccion de virus en plantas.

Info

Publication number
ES2192945A1
ES2192945A1 ES200101593A ES200101593A ES2192945A1 ES 2192945 A1 ES2192945 A1 ES 2192945A1 ES 200101593 A ES200101593 A ES 200101593A ES 200101593 A ES200101593 A ES 200101593A ES 2192945 A1 ES2192945 A1 ES 2192945A1
Authority
ES
Spain
Prior art keywords
interfering
plant
whose
plants
virus infection
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
ES200101593A
Other languages
English (en)
Other versions
ES2192945B1 (es
Inventor
Peralo Francisco Tenllado
Ruiz Jose Ramon Diaz
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Consejo Superior de Investigaciones Cientificas CSIC
Original Assignee
Consejo Superior de Investigaciones Cientificas CSIC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Consejo Superior de Investigaciones Cientificas CSIC filed Critical Consejo Superior de Investigaciones Cientificas CSIC
Priority to ES200101593A priority Critical patent/ES2192945B1/es
Priority to PCT/ES2002/000319 priority patent/WO2003004649A1/es
Priority to EP02743278A priority patent/EP1416049A1/en
Publication of ES2192945A1 publication Critical patent/ES2192945A1/es
Application granted granted Critical
Publication of ES2192945B1 publication Critical patent/ES2192945B1/es
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8283—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for virus resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

Un método para interferir con la infección de virus en plantas. Se revela un método para interferir o degradar de forma especifica (preferentemente mediante silenciamiento génico) cualquier secuencia nucleotídica de RNA de cadena sencilla (ssRNA) (preferentemente RNA mensajeros de virus, de genes endógenos o de transgenes) en las células de cualquier planta, cuyo método consiste en la aplicación directa en cualquier tejido de la planta (preferentemente hojas) y por cualquier método de aplicación (preferentemente inoculación o infiltración) de moléculas de RNA de doble cadena (dsRNA) producidas por cualquier procedimiento natural o sintético (preferentemente mediante transcripción in vitro) cuyas moléculas de dsRNA tienen suficiente similitud de secuencia con cualquier fragmento del ssRNA que se quiere degradar, o cuya expresión se quiere silenciar, en la planta.
ES200101593A 2001-07-06 2001-07-06 Un metodo para interferir con la infeccion de virus en plantas. Expired - Fee Related ES2192945B1 (es)

Priority Applications (3)

Application Number Priority Date Filing Date Title
ES200101593A ES2192945B1 (es) 2001-07-06 2001-07-06 Un metodo para interferir con la infeccion de virus en plantas.
PCT/ES2002/000319 WO2003004649A1 (es) 2001-07-06 2002-06-28 Un método para interferir con la infección de virus en plantas
EP02743278A EP1416049A1 (en) 2001-07-06 2002-06-28 Method of interfering with a virus infection in plants

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES200101593A ES2192945B1 (es) 2001-07-06 2001-07-06 Un metodo para interferir con la infeccion de virus en plantas.

Publications (2)

Publication Number Publication Date
ES2192945A1 true ES2192945A1 (es) 2003-10-16
ES2192945B1 ES2192945B1 (es) 2005-03-01

Family

ID=8498334

Family Applications (1)

Application Number Title Priority Date Filing Date
ES200101593A Expired - Fee Related ES2192945B1 (es) 2001-07-06 2001-07-06 Un metodo para interferir con la infeccion de virus en plantas.

Country Status (3)

Country Link
EP (1) EP1416049A1 (es)
ES (1) ES2192945B1 (es)
WO (1) WO2003004649A1 (es)

Families Citing this family (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4505626B2 (ja) * 2003-12-15 2010-07-21 独立行政法人農業生物資源研究所 花粉特異的発現活性を有するプロモーター
JP4505628B2 (ja) * 2003-12-15 2010-07-21 独立行政法人農業生物資源研究所 葉特異的発現活性を有するプロモーター
JP4505627B2 (ja) * 2003-12-15 2010-07-21 独立行政法人農業生物資源研究所 緑色組織特異的発現活性を有するプロモーター
EP1807522B1 (en) 2004-10-21 2013-11-20 Venganza Inc. Methods and materials for conferring resistance to pests and pathogens of plants
US8097712B2 (en) * 2007-11-07 2012-01-17 Beelogics Inc. Compositions for conferring tolerance to viral disease in social insects, and the use thereof
MX352992B (es) 2009-05-05 2017-12-15 Beeologics Inc Prevencion y tratamiento de la enfermedad de nosema en abejas.
MX2012002189A (es) 2009-08-21 2012-03-16 Beeologics Inc Prevencion y cura de las enfermedades de los insectos beneficiosos a traves de moleculas.
US8962584B2 (en) 2009-10-14 2015-02-24 Yissum Research Development Company Of The Hebrew University Of Jerusalem, Ltd. Compositions for controlling Varroa mites in bees
PE20130649A1 (es) * 2010-03-08 2013-07-13 Monsanto Technology Llc Moleculas polinucleotidicas para regulacion genetica en plantas
EP2755466A4 (en) 2011-09-13 2015-04-15 Monsanto Technology Llc METHOD AND COMPOSITIONS FOR WEED CONTROL
US10829828B2 (en) 2011-09-13 2020-11-10 Monsanto Technology Llc Methods and compositions for weed control
WO2013040116A1 (en) 2011-09-13 2013-03-21 Monsanto Technology Llc Methods and compositions for weed control
US10806146B2 (en) 2011-09-13 2020-10-20 Monsanto Technology Llc Methods and compositions for weed control
US10760086B2 (en) 2011-09-13 2020-09-01 Monsanto Technology Llc Methods and compositions for weed control
MX343072B (es) 2011-09-13 2016-10-21 Monsanto Technology Llc Metodos y composiciones para controlar malezas.
US9840715B1 (en) 2011-09-13 2017-12-12 Monsanto Technology Llc Methods and compositions for delaying senescence and improving disease tolerance and yield in plants
CN103957697B (zh) 2011-09-13 2017-10-24 孟山都技术公司 用于杂草控制的方法和组合物
US9920326B1 (en) 2011-09-14 2018-03-20 Monsanto Technology Llc Methods and compositions for increasing invertase activity in plants
US10240162B2 (en) 2012-05-24 2019-03-26 A.B. Seeds Ltd. Compositions and methods for silencing gene expression
AR093058A1 (es) 2012-10-18 2015-05-13 Monsanto Technology Llc Metodos y composiciones para el control de pestes en plantas
US10683505B2 (en) 2013-01-01 2020-06-16 Monsanto Technology Llc Methods of introducing dsRNA to plant seeds for modulating gene expression
UA118841C2 (uk) * 2013-01-01 2019-03-25 Ей.Бі. СІДС ЛТД. Спосіб формування рослини, яка має підвищену стійкість до комахи-шкідника
US10000767B2 (en) 2013-01-28 2018-06-19 Monsanto Technology Llc Methods and compositions for plant pest control
WO2014164761A1 (en) 2013-03-13 2014-10-09 Monsanto Technology Llc Methods and compositions for weed control
EP2967082A4 (en) 2013-03-13 2016-11-02 Monsanto Technology Llc METHOD AND COMPOSITIONS FOR WEED CONTROL
US20140283211A1 (en) 2013-03-14 2014-09-18 Monsanto Technology Llc Methods and Compositions for Plant Pest Control
US10568328B2 (en) 2013-03-15 2020-02-25 Monsanto Technology Llc Methods and compositions for weed control
US9850496B2 (en) 2013-07-19 2017-12-26 Monsanto Technology Llc Compositions and methods for controlling Leptinotarsa
JP6668236B2 (ja) 2013-07-19 2020-03-18 モンサント テクノロジー エルエルシー Ｌｅｐｔｉｎｏｔａｒｓａ防除用組成物及びその方法
MX390055B (es) 2013-11-04 2025-03-20 Monsanto Technology Llc Composiciones y metodos para controlar infestaciones de plagas y parasitos de los artropodos.
UA119253C2 (uk) 2013-12-10 2019-05-27 Біолоджикс, Інк. Спосіб боротьби із вірусом у кліща varroa та у бджіл
ES2751497T3 (es) 2013-12-20 2020-03-31 Univ Queensland Composiciones de iARN de protección de plantas que comprenden ARN bicatenario de protección de plantas adsorbido en partículas de hidróxido doble en capas
CN105979770B (zh) 2014-01-15 2019-07-05 孟山都技术公司 用于使用epsps多核苷酸的杂草控制的方法和组合物
BR112016022711A2 (pt) 2014-04-01 2017-10-31 Monsanto Technology Llc composições e métodos para controle de pragas de inseto
WO2015200223A1 (en) 2014-06-23 2015-12-30 Monsanto Technology Llc Compositions and methods for regulating gene expression via rna interference
US11807857B2 (en) 2014-06-25 2023-11-07 Monsanto Technology Llc Methods and compositions for delivering nucleic acids to plant cells and regulating gene expression
AU2015296700B2 (en) 2014-07-29 2021-10-21 Monsanto Technology Llc Compositions and methods for controlling insect pests
US10968449B2 (en) 2015-01-22 2021-04-06 Monsanto Technology Llc Compositions and methods for controlling Leptinotarsa
US10883103B2 (en) 2015-06-02 2021-01-05 Monsanto Technology Llc Compositions and methods for delivery of a polynucleotide into a plant
EP3302030A4 (en) * 2015-06-03 2019-04-24 Monsanto Technology LLC METHOD AND COMPOSITIONS FOR INTRODUCING NUCLEIC ACIDS IN PLANTS
MX370604B (es) 2015-06-19 2019-12-18 Univ Queensland Composicion.
WO2019051232A1 (en) 2017-09-11 2019-03-14 Altria Client Services Llc COMPOSITIONS AND METHODS FOR PRODUCING TOBACCO PLANTS AND PRODUCTS HAVING REDUCED OR ELIMINATED GOURMETS

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999032619A1 (en) * 1997-12-23 1999-07-01 The Carnegie Institution Of Washington Genetic inhibition by double-stranded rna
WO1999061631A1 (en) * 1998-05-26 1999-12-02 Novartis Ag Dsrna-mediated regulation of gene expression in plants
WO2000044914A1 (en) * 1999-01-28 2000-08-03 Medical College Of Georgia Research Institute, Inc. Composition and method for in vivo and in vitro attenuation of gene expression using double stranded rna

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001037654A2 (en) * 1999-11-24 2001-05-31 Dna Plant Technology Corporation METHOD OF EXPRESSING dsRNA IN PLANTS TO INHIBIT INSECT PESTS

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999032619A1 (en) * 1997-12-23 1999-07-01 The Carnegie Institution Of Washington Genetic inhibition by double-stranded rna
WO1999061631A1 (en) * 1998-05-26 1999-12-02 Novartis Ag Dsrna-mediated regulation of gene expression in plants
WO2000044914A1 (en) * 1999-01-28 2000-08-03 Medical College Of Georgia Research Institute, Inc. Composition and method for in vivo and in vitro attenuation of gene expression using double stranded rna

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
WATERHOUSE et al. "Virus resistance and gene silencing in plants can be induced by simultaneous expression of sense and antisense RNA" Proceedings of the National Academy of USA, (1998), Vol. 95, pßginas 13959-13964. *

Also Published As

Publication number Publication date
EP1416049A1 (en) 2004-05-06
ES2192945B1 (es) 2005-03-01
WO2003004649A1 (es) 2003-01-16

Similar Documents

Publication Publication Date Title
ES2192945B1 (es) Un metodo para interferir con la infeccion de virus en plantas.
WO2020198509A3 (en) Modified oligonucleotides with increased stability
PA8813901A1 (es) Paramixovirus oncolíticos atenuados que codifican citoquinas de aves
AU2003261231A8 (en) Modified small interfering rna molecules and methods of use
CY1119062T1 (el) Μικρα rna μορια που προκαλουν παρεμβαση rna
ATE526407T1 (de) Insektenresistenz durch inhibierung von genexpression
EP1470257A4 (en) RNA INTERFERENCE-MEDIATED INHIBITION OF EXPRESSION OF THE GENE FOR NF-KAPPA B / REL-A USING SINA (SHORT INTERFERING NUCLEIC ACID)
BRPI0516874A (pt) micrornas
Vetukuri et al. Evidence for involvement of Dicer‐like, Argonaute and histone deacetylase proteins in gene silencing in Phytophthora infestans
NO20083208L (no) RNA-interferensmediert inhibering av hepatitt C-virus-genekspresjon ved anvendelse av korte interfererende nukleinsyrer (siNA)
Shimura et al. Viral induction and suppression of RNA silencing in plants
Yan et al. Characterization of siRNAs derived from rice stripe virus in infected rice plants by deep sequencing
AR079710A1 (es) Proceso para el cultivo de celulas adherentes
HUP0102103A2 (hu) A génexpresszió szabályozása növényekben dsRNS segítségével
BR112014010134A2 (pt) inibição da expressão genética viral
AR079813A1 (es) Metodos para controlar el nematodo de las lesiones de las raices
Li et al. Different virus-derived siRNAs profiles between leaves and fruits in cucumber green mottle mosaic virus-infected Lagenaria siceraria plants
MX2020010802A (es) Moleculas inhibidoras de acidos nucleicos de caracter bicatenario modificadas con nucleotidos que aumentan la tm.
Lin et al. Global analyses of small interfering RNAs derived from Bamboo mosaic virus and its associated satellite RNAs in different plants
Sundaresha et al. In vitro method for synthesis of large-scale dsRNA molecule as a novel plant protection strategy
RU2016131028A (ru) Композиции, содержащие асимметричную интерферирующую рнк, которые подвергают сайленсингу k-ras, и способы их применения
Cao et al. Integrative analysis of the RNA interference toolbox in two Salicaceae willow species, and their roles in stress response in poplar (Populus trichocarpa Torr. & Gray)
Folimonova et al. Superinfection exclusion by Citrus tristeza virus does not correlate with the production of viral small RNAs
CL2021002842A1 (es) Moléculas inhibidoras de ácido nucleico bicatenario con cadenas de sentido acortadas
Velázquez et al. Association of citrus psorosis B symptoms with a sequence variant of the Citrus psorosis virus RNA 2

Legal Events

Date Code Title Description
EC2A Search report published

Date of ref document: 20031016

Kind code of ref document: A1

FG2A Definitive protection

Ref document number: 2192945B1

Country of ref document: ES

FD2A Announcement of lapse in spain

Effective date: 20180807