ES2194909T3 - Expresion de endotoxina delta quimerica en pseudomonas fluorescentes. - Google Patents

Expresion de endotoxina delta quimerica en pseudomonas fluorescentes.

Info

Publication number
ES2194909T3
ES2194909T3 ES95918907T ES95918907T ES2194909T3 ES 2194909 T3 ES2194909 T3 ES 2194909T3 ES 95918907 T ES95918907 T ES 95918907T ES 95918907 T ES95918907 T ES 95918907T ES 2194909 T3 ES2194909 T3 ES 2194909T3
Authority
ES
Spain
Prior art keywords
expression
protoxin
segment
chemerica
pseudomones
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES95918907T
Other languages
English (en)
Inventor
Mark Thompson
George E Schwab
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mycogen Corp
Original Assignee
Mycogen Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mycogen Corp filed Critical Mycogen Corp
Application granted granted Critical
Publication of ES2194909T3 publication Critical patent/ES2194909T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/50—Isolated enzymes; Isolated proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C07K14/325—Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Pest Control & Pesticides (AREA)
  • Dentistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Environmental Sciences (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Physics & Mathematics (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

LA EXPRESION DE LA ENDOTOXINA DE BACILLUS THURINGIENSIS EN PSEUDOMONAS SE PUEDE MEJORAR MODIFICANDO EL GEN QUE CODIFICA PARA LA ENDOTOXINA DE BACILLUS THURINGIENSIS. LOS GENES QUIMERICOS SE CREAN REEMPLAZANDO EL SEGMENTO DEL GEN DE BACILLUS THURINGIENSIS QUE CODIFICA PARA LA PROTOXINA NATIVA, POR UN SEGMENTO QUE CODIFIQUE PARA UNA PROTOXINA DIFERENTE. AQUI SE EJEMPLIFICA LA QUIMERA CRYIF/CRYI(B), EN LA QUE EL SEGMENTO NATIVO QUE CODIFICA PARA PROTOXINA CRYIF HA SIDO SUSTITUIDO POR EL SEGMENTO PARA PROTOXINA CRYI(B), PARA PRODUCIR UNA EXPRESION MEJORADA DE LA TOXINA CRYIF EN PSEUDOMONAS. LA INVENCION HACE ASIMISMO REFERENCIA A NUEVOS GENES Y PLASMIDOS.
ES95918907T 1994-05-06 1995-05-05 Expresion de endotoxina delta quimerica en pseudomonas fluorescentes. Expired - Lifetime ES2194909T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/239,476 US5527883A (en) 1994-05-06 1994-05-06 Delta-endotoxin expression in pseudomonas fluorescens

Publications (1)

Publication Number Publication Date
ES2194909T3 true ES2194909T3 (es) 2003-12-01

Family

ID=22902310

Family Applications (1)

Application Number Title Priority Date Filing Date
ES95918907T Expired - Lifetime ES2194909T3 (es) 1994-05-06 1995-05-05 Expresion de endotoxina delta quimerica en pseudomonas fluorescentes.

Country Status (11)

Country Link
US (2) US5527883A (es)
EP (1) EP0758385B1 (es)
JP (1) JP3878209B2 (es)
KR (1) KR100376096B1 (es)
AT (1) ATE236253T1 (es)
AU (1) AU697148B2 (es)
CA (1) CA2188795A1 (es)
DE (1) DE69530194T2 (es)
ES (1) ES2194909T3 (es)
NZ (1) NZ285524A (es)
WO (1) WO1995030753A1 (es)

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ES2161096B1 (es) * 1996-03-08 2003-02-16 Univ Catalunya Politecnica Aparato para la determinacion de la resistencia al paso de liquidos enlos tejidos.
US6063756A (en) 1996-09-24 2000-05-16 Monsanto Company Bacillus thuringiensis cryET33 and cryET34 compositions and uses therefor
US6110668A (en) * 1996-10-07 2000-08-29 Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. Gene synthesis method
US5965428A (en) * 1996-10-08 1999-10-12 Ecogen, Inc. Chimeric lepidopteran-toxic crystal proteins
AP9901541A0 (en) 1996-11-20 1999-06-30 Ecogen Inc Broad-spectrum delta-endotoxins.
US6713063B1 (en) 1996-11-20 2004-03-30 Monsanto Technology, Llc Broad-spectrum δ-endotoxins
US6017534A (en) 1996-11-20 2000-01-25 Ecogen, Inc. Hybrid Bacillus thuringiensis δ-endotoxins with novel broad-spectrum insecticidal activity
US6121436A (en) 1996-12-13 2000-09-19 Monsanto Company Antifungal polypeptide and methods for controlling plant pathogenic fungi
EP2325308A3 (en) * 1997-04-04 2011-07-27 The Board of Regents of the University of Nebraska Methods and materials for making and using transgenic dicamba-degrading organisms
US6218188B1 (en) * 1997-11-12 2001-04-17 Mycogen Corporation Plant-optimized genes encoding pesticidal toxins
EP1676922B1 (en) * 1997-11-12 2008-09-17 Mycogen Corporation Plant-optimized genes encoding pesticidal toxins
AU774176B2 (en) * 1997-11-12 2004-06-17 Mycogen Corporation Plant-optimised genes encoding pesticidal toxins
US6593299B1 (en) 1999-04-21 2003-07-15 University Of Florida Research Foundation, Inc. Compositions and methods for controlling pests
AU4463000A (en) 1999-04-21 2000-11-02 University Of Florida Research Foundation, Inc. Neuropeptides and their use for pest control
AU2002306484A1 (en) * 2002-02-13 2003-09-04 Dow Global Technologies Inc. Over-expression of extremozyme genes in pseudomonads and closely related bacteria
CA2482995C (en) * 2002-04-22 2013-01-29 Dow Global Technologies Inc. Low-cost production of peptides
US9453251B2 (en) 2002-10-08 2016-09-27 Pfenex Inc. Expression of mammalian proteins in Pseudomonas fluorescens
WO2005052151A1 (en) 2003-11-19 2005-06-09 Dow Global Technologies Inc. Improved protein expression systems
CA2546157C (en) 2003-11-21 2014-07-22 Dow Global Technolgies Inc. Improved expression systems with sec-system secretion
CA2547511A1 (en) * 2003-12-01 2005-07-28 Dow Global Technolgies Inc. Recombinant icosahedral virus like particle production in pseudomonads
EP1709170B1 (en) 2004-01-16 2018-02-21 Pfenex Inc. Expression of mammalian proteins in pseudomonas fluorescens
BRPI0513826A2 (pt) 2004-07-26 2010-06-22 Dow Global Technologies Inc processo para expressão de proteìna melhorada através de engenharia de cepa
EA015908B1 (ru) 2005-08-31 2011-12-30 Монсанто Текнолоджи, Ллс Инсектицидный белок в.thuringiensis cry1a.105, кодирующий его полинуклеотид и их применение
EP2021476B1 (en) 2006-05-26 2014-07-09 Monsanto Technology, LLC Corn plant and seed corresponding to transgenic event mon89034 and methods for detection and use thereof
EP2468869B1 (en) 2007-01-31 2015-03-18 Pfenex Inc. Bacterial leader sequences for increased expression
CN101784655A (zh) * 2007-04-27 2010-07-21 菲尼克斯股份有限公司 可溶性重组二十面体病毒样颗粒的改良生成和体内装配
US9580719B2 (en) 2007-04-27 2017-02-28 Pfenex, Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
US9394571B2 (en) 2007-04-27 2016-07-19 Pfenex Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
US20110020830A1 (en) * 2008-03-31 2011-01-27 Schneider Jane C Design for rapidly cloning one or more polypeptide chains into an expression system
AU2010247938B2 (en) 2009-05-11 2016-02-18 Pelican Technology Holdings, Inc. Production of recombinant proteins utilizing non-antibiotic selection methods and the incorporation of non-natural amino acids therein
BR112012014801B1 (pt) 2009-12-16 2024-03-05 Dow Agrosciences Llc Métodos para controlar o desenvolvimento da resistência de um inseto à uma proteína inseticida derivada de um bacillus thuringiensis, e para o controle de pragas de lepidópteros, bem como composição para o controle de pragas de lepidópteros
US20120324605A1 (en) * 2009-12-16 2012-12-20 Dow Agrosciences Llc Insectcidal protein combinations for controlling fall armyworm and european corn borer, and methods for insect resistance management
MX2014002027A (es) 2011-08-19 2014-11-10 Synthetic Genomics Inc Metodo integrado para identificacion de alto rendimiento de composiciones pesticidas novedosas y uso para el mismo.
PY1346216A (es) * 2012-10-05 2016-11-01 Dow Agrosciences Llc Uso de cry1ea en combinaciones para el control del insecto resistente gusano del ejército del otoño
WO2016057123A1 (en) 2014-08-28 2016-04-14 Dow Agrosciences Llc Dig-17 insecticidal cry toxins
CA3192494A1 (en) 2014-12-30 2016-07-07 Corteva Agriscience Llc Modified cry1ca toxins useful for control of insect pests
EP3240900A4 (en) * 2014-12-30 2018-06-06 Dow Agrosciences LLC Dig-305 insecticidal cry toxins
US10676723B2 (en) 2015-05-11 2020-06-09 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
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Also Published As

Publication number Publication date
WO1995030753A1 (en) 1995-11-16
KR100376096B1 (ko) 2003-10-10
AU2465295A (en) 1995-11-29
DE69530194D1 (de) 2003-05-08
NZ285524A (en) 1998-09-24
EP0758385A1 (en) 1997-02-19
DE69530194T2 (de) 2003-11-13
KR970702925A (ko) 1997-06-10
US5840554A (en) 1998-11-24
US5527883A (en) 1996-06-18
AU697148B2 (en) 1998-10-01
JP3878209B2 (ja) 2007-02-07
EP0758385B1 (en) 2003-04-02
JPH10500292A (ja) 1998-01-13
CA2188795A1 (en) 1995-11-16
ATE236253T1 (de) 2003-04-15

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