ES2314996T3 - Biosensor de glucosa. - Google Patents

Biosensor de glucosa. Download PDF

Info

Publication number: ES2314996T3
Authority: ES; Spain
Prior art keywords: gbp; glucose; vol; binding; indicator group
Prior art date: 1997-12-31
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired - Lifetime

Application number

ES98966130T

Other languages

English (en)

Spanish (es)

Inventor

Homme W. Hellinga

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Duke University

Original Assignee

Duke University

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1997-12-31

Filing date

1998-12-31

Publication date

2009-03-16

1998-12-31 Application filed by Duke University filed Critical Duke University

2009-03-16 Application granted granted Critical

2009-03-16 Publication of ES2314996T3 publication Critical patent/ES2314996T3/es

2018-12-31 Anticipated expiration legal-status Critical

Status Expired - Lifetime legal-status Critical Current

Links

WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 title claims abstract description 67
239000008103 glucose Substances 0.000 title claims abstract description 67
108090000623 proteins and genes Proteins 0.000 claims abstract description 33
102000004169 proteins and genes Human genes 0.000 claims abstract description 30
238000001514 detection method Methods 0.000 claims abstract description 9
241000588724 Escherichia coli Species 0.000 claims description 7
230000007246 mechanism Effects 0.000 claims description 7
230000008879 allosteric coupling Effects 0.000 claims description 3
230000000694 effects Effects 0.000 claims description 3
125000000539 amino acid group Chemical group 0.000 claims description 2
102000036202 glucose binding proteins Human genes 0.000 claims 14
108091011004 glucose binding proteins Proteins 0.000 claims 14
230000026683 transduction Effects 0.000 claims 1
238000010361 transduction Methods 0.000 claims 1
230000035772 mutation Effects 0.000 abstract description 9
239000008280 blood Substances 0.000 abstract description 4
210000004369 blood Anatomy 0.000 abstract description 4
230000004151 fermentation Effects 0.000 abstract description 2
238000009655 industrial fermentation Methods 0.000 abstract description 2
125000006853 reporter group Chemical group 0.000 abstract 1
235000018102 proteins Nutrition 0.000 description 25
230000008859 change Effects 0.000 description 23
239000003446 ligand Substances 0.000 description 21
230000003281 allosteric effect Effects 0.000 description 19
230000003993 interaction Effects 0.000 description 12
230000008878 coupling Effects 0.000 description 10
238000010168 coupling process Methods 0.000 description 10
238000005859 coupling reaction Methods 0.000 description 10
BEDDXHFONHIYPB-UHFFFAOYSA-N 2-[methyl-(4-nitro-2,1,3-benzoxadiazol-7-yl)amino]ethyl 2-iodoacetate Chemical compound ICC(=O)OCCN(C)C1=CC=C([N+]([O-])=O)C2=NON=C12 BEDDXHFONHIYPB-UHFFFAOYSA-N 0.000 description 9
HMWAJFNEGAJETK-UHFFFAOYSA-N 1-[6-(dimethylamino)naphthalen-2-yl]prop-2-en-1-one Chemical compound C1=C(C(=O)C=C)C=CC2=CC(N(C)C)=CC=C21 HMWAJFNEGAJETK-UHFFFAOYSA-N 0.000 description 8
239000002904 solvent Substances 0.000 description 8
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 6
239000002609 medium Substances 0.000 description 6
XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 description 5
238000013459 approach Methods 0.000 description 5
235000018417 cysteine Nutrition 0.000 description 5
238000013461 design Methods 0.000 description 5
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229930182830 galactose Natural products 0.000 description 5
239000001257 hydrogen Substances 0.000 description 5
229910052739 hydrogen Inorganic materials 0.000 description 5
239000000126 substance Substances 0.000 description 5
102000008300 Mutant Proteins Human genes 0.000 description 4
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XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 4
230000005284 excitation Effects 0.000 description 4
XMBWDFGMSWQBCA-UHFFFAOYSA-N hydrogen iodide Chemical compound I XMBWDFGMSWQBCA-UHFFFAOYSA-N 0.000 description 4
RAXXELZNTBOGNW-UHFFFAOYSA-N imidazole Natural products C1=CNC=N1 RAXXELZNTBOGNW-UHFFFAOYSA-N 0.000 description 4
238000000034 method Methods 0.000 description 4
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231100000350 mutagenesis Toxicity 0.000 description 4
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OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 3
WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 3
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
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GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 3
238000002835 absorbance Methods 0.000 description 3
WQZGKKKJIJFFOK-PHYPRBDBSA-N alpha-D-galactose Chemical compound OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-PHYPRBDBSA-N 0.000 description 3
WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 3
238000005119 centrifugation Methods 0.000 description 3
239000013078 crystal Substances 0.000 description 3
206010012601 diabetes mellitus Diseases 0.000 description 3
238000010353 genetic engineering Methods 0.000 description 3
238000001597 immobilized metal affinity chromatography Methods 0.000 description 3
MQZFZDIZKWNWFX-UHFFFAOYSA-N osmium(2+) Chemical compound [Os+2] MQZFZDIZKWNWFX-UHFFFAOYSA-N 0.000 description 3
125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 3
239000000523 sample Substances 0.000 description 3
230000019491 signal transduction Effects 0.000 description 3
239000011780 sodium chloride Substances 0.000 description 3
239000000243 solution Substances 0.000 description 3
150000003573 thiols Chemical group 0.000 description 3
-1 (2- (iodoacetoxy) ethyl) methyl Chemical group 0.000 description 2
108020004414 DNA Proteins 0.000 description 2
QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 2
229910019142 PO4 Inorganic materials 0.000 description 2
XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 2
108091081024 Start codon Proteins 0.000 description 2
235000004279 alanine Nutrition 0.000 description 2
230000008877 allosteric signaling Effects 0.000 description 2
QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
239000001045 blue dye Substances 0.000 description 2
102220421658 c.461A>C Human genes 0.000 description 2
230000001419 dependent effect Effects 0.000 description 2
238000000295 emission spectrum Methods 0.000 description 2
238000001506 fluorescence spectroscopy Methods 0.000 description 2
239000003269 fluorescent indicator Substances 0.000 description 2
238000005558 fluorometry Methods 0.000 description 2
235000013305 food Nutrition 0.000 description 2
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230000003287 optical effect Effects 0.000 description 2
239000001301 oxygen Substances 0.000 description 2
229910052760 oxygen Inorganic materials 0.000 description 2
NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 2
239000010452 phosphate Substances 0.000 description 2
229910052710 silicon Inorganic materials 0.000 description 2
239000010703 silicon Substances 0.000 description 2
239000000758 substrate Substances 0.000 description 2
238000006257 total synthesis reaction Methods 0.000 description 2
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
KIUMMUBSPKGMOY-UHFFFAOYSA-N 3,3'-Dithiobis(6-nitrobenzoic acid) Chemical compound C1=C([N+]([O-])=O)C(C(=O)O)=CC(SSC=2C=C(C(=CC=2)[N+]([O-])=O)C(O)=O)=C1 KIUMMUBSPKGMOY-UHFFFAOYSA-N 0.000 description 1
QFVHZQCOUORWEI-UHFFFAOYSA-N 4-[(4-anilino-5-sulfonaphthalen-1-yl)diazenyl]-5-hydroxynaphthalene-2,7-disulfonic acid Chemical compound C=12C(O)=CC(S(O)(=O)=O)=CC2=CC(S(O)(=O)=O)=CC=1N=NC(C1=CC=CC(=C11)S(O)(=O)=O)=CC=C1NC1=CC=CC=C1 QFVHZQCOUORWEI-UHFFFAOYSA-N 0.000 description 1
102000006410 Apoproteins Human genes 0.000 description 1
108010083590 Apoproteins Proteins 0.000 description 1
241000894006 Bacteria Species 0.000 description 1
101100037309 Enterobacteria phage T4 Y10A gene Proteins 0.000 description 1
102000004190 Enzymes Human genes 0.000 description 1
108090000790 Enzymes Proteins 0.000 description 1
229910002601 GaN Inorganic materials 0.000 description 1
JMASRVWKEDWRBT-UHFFFAOYSA-N Gallium nitride Chemical compound [Ga]#N JMASRVWKEDWRBT-UHFFFAOYSA-N 0.000 description 1
UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
108091034117 Oligonucleotide Proteins 0.000 description 1
108010038807 Oligopeptides Proteins 0.000 description 1
102000015636 Oligopeptides Human genes 0.000 description 1
108010092494 Periplasmic binding proteins Proteins 0.000 description 1
102000006335 Phosphate-Binding Proteins Human genes 0.000 description 1
108010058514 Phosphate-Binding Proteins Proteins 0.000 description 1
241000269907 Pleuronectes platessa Species 0.000 description 1
108020005038 Terminator Codon Proteins 0.000 description 1
102220481255 Thymocyte selection-associated high mobility group box protein TOX_Y10A_mutation Human genes 0.000 description 1
HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
230000004075 alteration Effects 0.000 description 1
235000001014 amino acid Nutrition 0.000 description 1
150000001413 amino acids Chemical class 0.000 description 1
AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 1
229960000723 ampicillin Drugs 0.000 description 1
239000012491 analyte Substances 0.000 description 1
238000004458 analytical method Methods 0.000 description 1
239000012736 aqueous medium Substances 0.000 description 1
239000007853 buffer solution Substances 0.000 description 1
238000004364 calculation method Methods 0.000 description 1
230000001413 cellular effect Effects 0.000 description 1
238000006243 chemical reaction Methods 0.000 description 1
230000000295 complement effect Effects 0.000 description 1
238000010276 construction Methods 0.000 description 1
238000005520 cutting process Methods 0.000 description 1
150000001945 cysteines Chemical class 0.000 description 1
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239000010432 diamond Substances 0.000 description 1
238000004090 dissolution Methods 0.000 description 1
238000009510 drug design Methods 0.000 description 1
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238000010828 elution Methods 0.000 description 1
238000005516 engineering process Methods 0.000 description 1
239000013604 expression vector Substances 0.000 description 1
108010090623 galactose binding protein Proteins 0.000 description 1
238000002523 gelfiltration Methods 0.000 description 1
150000002303 glucose derivatives Chemical class 0.000 description 1
238000003384 imaging method Methods 0.000 description 1
NBZBKCUXIYYUSX-UHFFFAOYSA-N iminodiacetic acid Chemical compound OC(=O)CNCC(O)=O NBZBKCUXIYYUSX-UHFFFAOYSA-N 0.000 description 1
230000003116 impacting effect Effects 0.000 description 1
239000003999 initiator Substances 0.000 description 1
229910052816 inorganic phosphate Inorganic materials 0.000 description 1
230000010354 integration Effects 0.000 description 1
238000004020 luminiscence type Methods 0.000 description 1
239000006166 lysate Substances 0.000 description 1
229920002521 macromolecule Polymers 0.000 description 1
229910052751 metal Inorganic materials 0.000 description 1
239000002184 metal Substances 0.000 description 1
239000000203 mixture Substances 0.000 description 1
239000003068 molecular probe Substances 0.000 description 1
229910052762 osmium Inorganic materials 0.000 description 1
SYQBFIAQOQZEGI-UHFFFAOYSA-N osmium atom Chemical compound [Os] SYQBFIAQOQZEGI-UHFFFAOYSA-N 0.000 description 1
XQBKHDFIPARBOX-UHFFFAOYSA-N osmium(3+) Chemical compound [Os+3] XQBKHDFIPARBOX-UHFFFAOYSA-N 0.000 description 1
210000000496 pancreas Anatomy 0.000 description 1
230000001575 pathological effect Effects 0.000 description 1
230000002093 peripheral effect Effects 0.000 description 1
239000008055 phosphate buffer solution Substances 0.000 description 1
230000004962 physiological condition Effects 0.000 description 1
239000013612 plasmid Substances 0.000 description 1
229920002401 polyacrylamide Polymers 0.000 description 1
238000002360 preparation method Methods 0.000 description 1
108090000765 processed proteins & peptides Proteins 0.000 description 1
238000012545 processing Methods 0.000 description 1
238000000159 protein binding assay Methods 0.000 description 1
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102000005962 receptors Human genes 0.000 description 1
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150000003839 salts Chemical class 0.000 description 1
239000012898 sample dilution Substances 0.000 description 1
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Classifications

- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/66—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood sugars, e.g. galactose
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/808—Optical sensing apparatus
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/817—Enzyme or microbe electrode
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/805—Optical property

Landscapes

Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Engineering & Computer Science (AREA)
Hematology (AREA)
Molecular Biology (AREA)
Biomedical Technology (AREA)
Chemical & Material Sciences (AREA)
Immunology (AREA)
Urology & Nephrology (AREA)
Food Science & Technology (AREA)
General Physics & Mathematics (AREA)
Cell Biology (AREA)
Biotechnology (AREA)
Medicinal Chemistry (AREA)
Physics & Mathematics (AREA)
Analytical Chemistry (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
Microbiology (AREA)
Pathology (AREA)
Diabetes (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Investigating Or Analysing Biological Materials (AREA)
Peptides Or Proteins (AREA)
Measurement Of The Respiration, Hearing Ability, Form, And Blood Characteristics Of Living Organisms (AREA)
Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

ES98966130T 1997-12-31 1998-12-31 Biosensor de glucosa. Expired - Lifetime ES2314996T3 (es)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
US7029397P	1997-12-31	1997-12-31
US70293P		1997-12-31

Publications (1)

Publication Number	Publication Date
ES2314996T3 true ES2314996T3 (es)	2009-03-16

Family

ID=22094408

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
ES98966130T Expired - Lifetime ES2314996T3 (es)	1997-12-31	1998-12-31	Biosensor de glucosa.

Country Status (10)

Country	Link
US (2)	US6277627B1 (de)
EP (1)	EP1044374B1 (de)
JP (1)	JP4472169B2 (de)
AT (1)	ATE410683T1 (de)
AU (1)	AU760743B2 (de)
CA (1)	CA2316199C (de)
DE (1)	DE69840107D1 (de)
DK (1)	DK1044374T3 (de)
ES (1)	ES2314996T3 (de)
WO (1)	WO1999034212A1 (de)

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US6535753B1 (en) *	1998-08-20	2003-03-18	Microsense International, Llc	Micro-invasive method for painless detection of analytes in extra-cellular space
FR2805820B1 (fr) *	2000-03-01	2009-09-11	Pasteur Institut	Biocapteurs, leur procede d'obtention et leurs applications
JP4647742B2 (ja) *	2000-03-13	2011-03-09	三菱化学メディエンス株式会社	アスコルビン酸の分析方法及び分析用試薬
JP2002250730A (ja) *	2001-02-27	2002-09-06	Shinwa Kako Kk	分子センサー及びその製造方法
US20100248268A1 (en) *	2001-03-27	2010-09-30	Woods Daniel F	Methods to utilize invertebrate chemosensory proteins for industrial and commercial uses
WO2003021247A1 (en) *	2001-08-28	2003-03-13	Duke University	Biosensor
US6767733B1 (en)	2001-10-10	2004-07-27	Pritest, Inc.	Portable biosensor apparatus with controlled flow
US20050239155A1 (en) *	2002-01-04	2005-10-27	Javier Alarcon	Entrapped binding protein as biosensors
US20030153026A1 (en) *	2002-01-04	2003-08-14	Javier Alarcon	Entrapped binding protein as biosensors
US6855556B2 (en)	2002-01-04	2005-02-15	Becton, Dickinson And Company	Binding protein as biosensors
US7851593B2 (en) *	2002-01-04	2010-12-14	Becton, Dickinson And Company	Binding proteins as biosensors
US7064103B2 (en) *	2002-01-04	2006-06-20	Becton, Dickinson And Company	Binding protein as biosensors
US20030232370A1 (en) *	2002-04-22	2003-12-18	Trifiro Mark A.	Glucose sensor and uses thereof
US20040060987A1 (en) *	2002-05-07	2004-04-01	Green Larry R.	Digital image analysis method for enhanced and optimized signals in fluorophore detection
DE60326256D1 (de) *	2002-10-16	2009-04-02	Univ Duke	Biosensor zum nachweis von glukose
AU2003293959A1 (en) *	2002-12-20	2004-07-14	Julius-Maximilians-Uni Versitat Wurzburg	Millisecond activation switch for seven-transmembrane proteins
US20040234962A1 (en) *	2003-05-02	2004-11-25	Javier Alarcon	Multicoated or multilayer entrapment matrix for protein biosensor
EP1620548A2 (de) *	2003-05-07	2006-02-01	Duke University	Proteinkonstruktion für rezeptor-liganden-erkennung und -bindung
US7718353B2 (en) *	2003-05-09	2010-05-18	University Of Maryland Baltimore County	Proteins, sensors, and methods of characterizing analytes using the same
US20050112685A1 (en) *	2003-11-26	2005-05-26	Amiss Terry J.	Compositions and methods for measuring analyte concentrations
US7496392B2 (en)	2003-11-26	2009-02-24	Becton, Dickinson And Company	Fiber optic device for sensing analytes
US7787923B2 (en) *	2003-11-26	2010-08-31	Becton, Dickinson And Company	Fiber optic device for sensing analytes and method of making same
US7563891B2 (en) *	2004-05-21	2009-07-21	Becton, Dickinson & Company	Long wavelength thiol-reactive fluorophores
US8685724B2 (en)	2004-06-01	2014-04-01	Kwalata Trading Limited	In vitro techniques for use with stem cells
JP4799557B2 (ja) *	2004-06-09	2011-10-26	ベクトン・ディキンソン・アンド・カンパニー	多検体センサー
DE102004029941B3 (de) *	2004-06-21	2005-12-15	Infineon Technologies Ag	System zur Auswertung eines Sensorsignals
US7951357B2 (en) *	2004-07-14	2011-05-31	Glusense Ltd.	Implantable power sources and sensors
US8530633B2 (en) *	2004-10-14	2013-09-10	Carnegie Institution Of Washington	Development of sensitive FRET sensors and methods of using the same
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DE102005007723A1 (de)	2005-02-18	2006-08-24	Heinrich Meurer	Luftgestütztes Verfahren zur Detektion von Landminen
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1998
- 1998-12-31 EP EP98966130A patent/EP1044374B1/de not_active Expired - Lifetime
- 1998-12-31 JP JP2000526812A patent/JP4472169B2/ja not_active Expired - Fee Related
- 1998-12-31 US US09/223,775 patent/US6277627B1/en not_active Expired - Lifetime
- 1998-12-31 CA CA2316199A patent/CA2316199C/en not_active Expired - Fee Related
- 1998-12-31 WO PCT/US1998/027882 patent/WO1999034212A1/en not_active Ceased
- 1998-12-31 AT AT98966130T patent/ATE410683T1/de not_active IP Right Cessation
- 1998-12-31 ES ES98966130T patent/ES2314996T3/es not_active Expired - Lifetime
- 1998-12-31 DE DE69840107T patent/DE69840107D1/de not_active Expired - Lifetime
- 1998-12-31 AU AU22101/99A patent/AU760743B2/en not_active Ceased
- 1998-12-31 DK DK98966130T patent/DK1044374T3/da active
2001
- 2001-06-27 US US09/891,307 patent/US6521446B2/en not_active Expired - Lifetime

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Publication number	Publication date
US6277627B1 (en)	2001-08-21
AU760743B2 (en)	2003-05-22
JP4472169B2 (ja)	2010-06-02
CA2316199A1 (en)	1999-07-08
US20020004217A1 (en)	2002-01-10
DE69840107D1 (de)	2008-11-20
ATE410683T1 (de)	2008-10-15
WO1999034212A1 (en)	1999-07-08
DK1044374T3 (da)	2009-02-16
JP2002500361A (ja)	2002-01-08
EP1044374A4 (de)	2002-11-27
CA2316199C (en)	2012-03-27
US6521446B2 (en)	2003-02-18
EP1044374A1 (de)	2000-10-18
EP1044374B1 (de)	2008-10-08
AU2210199A (en)	1999-07-19

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