ES2339227T3 - Acidos nucleicos que codifican antigenos recombinantes de 56 y 82 kda de gametocitos de eimeria maxima y sus usos. - Google Patents

Acidos nucleicos que codifican antigenos recombinantes de 56 y 82 kda de gametocitos de eimeria maxima y sus usos. Download PDF

Info

Publication number: ES2339227T3
Authority: ES; Spain
Prior art keywords: eimeria; kda; polypeptide; nucleic acid; vaccine
Prior art date: 2001-07-06
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired - Lifetime

Application number

ES02746870T

Other languages

English (en)

Spanish (es)

Inventor

Sabina L. Belli

Nicolas C. Smith

Michael Wallach

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Abic Biological Laboratories Ltd

Original Assignee

Abic Biological Laboratories Ltd

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2001-07-06

Filing date

2002-07-03

Publication date

2010-05-18

2002-07-03 Application filed by Abic Biological Laboratories Ltd filed Critical Abic Biological Laboratories Ltd

2010-05-18 Application granted granted Critical

2010-05-18 Publication of ES2339227T3 publication Critical patent/ES2339227T3/es

2022-07-03 Anticipated expiration legal-status Critical

Status Expired - Lifetime legal-status Critical Current

Links

241000223934 Eimeria maxima Species 0.000 title claims abstract description 108
150000007523 nucleic acids Chemical class 0.000 title claims abstract description 98
108020004707 nucleic acids Proteins 0.000 title claims abstract description 96
102000039446 nucleic acids Human genes 0.000 title claims abstract description 96
239000000427 antigen Substances 0.000 title claims description 152
108091007433 antigens Proteins 0.000 title claims description 152
102000036639 antigens Human genes 0.000 title claims description 152
125000003729 nucleotide group Chemical group 0.000 claims abstract description 180
239000002773 nucleotide Substances 0.000 claims abstract description 178
108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 176
102000004196 processed proteins & peptides Human genes 0.000 claims abstract description 150
229920001184 polypeptide Polymers 0.000 claims abstract description 134
210000000973 gametocyte Anatomy 0.000 claims abstract description 119
239000013612 plasmid Substances 0.000 claims abstract description 44
150000001413 amino acids Chemical class 0.000 claims abstract description 27
230000000295 complement effect Effects 0.000 claims abstract description 24
238000009396 hybridization Methods 0.000 claims abstract description 16
241000287828 Gallus gallus Species 0.000 claims description 93
235000013330 chicken meat Nutrition 0.000 claims description 83
229960005486 vaccine Drugs 0.000 claims description 69
125000003275 alpha amino acid group Chemical group 0.000 claims description 47
210000004027 cell Anatomy 0.000 claims description 46
210000003250 oocyst Anatomy 0.000 claims description 40
239000002299 complementary DNA Substances 0.000 claims description 36
108020004414 DNA Proteins 0.000 claims description 34
241000271566 Aves Species 0.000 claims description 33
241000223932 Eimeria tenella Species 0.000 claims description 27
208000015181 infectious disease Diseases 0.000 claims description 27
241000272517 Anseriformes Species 0.000 claims description 25
241000286209 Phasianidae Species 0.000 claims description 25
241000223924 Eimeria Species 0.000 claims description 24
238000000034 method Methods 0.000 claims description 23
241000223931 Eimeria acervulina Species 0.000 claims description 22
241000499563 Eimeria necatrix Species 0.000 claims description 20
241000499566 Eimeria brunetti Species 0.000 claims description 19
241000179199 Eimeria mitis Species 0.000 claims description 19
241000499544 Eimeria praecox Species 0.000 claims description 19
239000013598 vector Substances 0.000 claims description 18
230000001580 bacterial effect Effects 0.000 claims description 14
241000894006 Bacteria Species 0.000 claims description 13
241000272201 Columbiformes Species 0.000 claims description 12
239000002253 acid Substances 0.000 claims description 12
230000003053 immunization Effects 0.000 claims description 12
230000008774 maternal effect Effects 0.000 claims description 12
238000002649 immunization Methods 0.000 claims description 11
238000004519 manufacturing process Methods 0.000 claims description 11
101710159910 Movement protein Proteins 0.000 claims description 10
101710164418 Movement protein TGB2 Proteins 0.000 claims description 9
102100021225 Serine hydroxymethyltransferase, cytosolic Human genes 0.000 claims description 9
230000036039 immunity Effects 0.000 claims description 8
241000251468 Actinopterygii Species 0.000 claims description 7
241000283690 Bos taurus Species 0.000 claims description 7
241001494479 Pecora Species 0.000 claims description 7
230000001939 inductive effect Effects 0.000 claims description 7
238000007918 intramuscular administration Methods 0.000 claims description 7
238000007912 intraperitoneal administration Methods 0.000 claims description 7
238000001990 intravenous administration Methods 0.000 claims description 7
238000002360 preparation method Methods 0.000 claims description 7
102000053602 DNA Human genes 0.000 claims description 6
230000028993 immune response Effects 0.000 claims description 6
241000282887 Suidae Species 0.000 claims description 5
238000012258 culturing Methods 0.000 claims description 5
210000001161 mammalian embryo Anatomy 0.000 claims description 5
238000005507 spraying Methods 0.000 claims description 4
241000238631 Hexapoda Species 0.000 claims description 3
210000003608 fece Anatomy 0.000 claims description 3
238000011534 incubation Methods 0.000 claims description 3
238000013518 transcription Methods 0.000 claims description 3
230000035897 transcription Effects 0.000 claims description 3
101710095306 Alpha-crystallin Proteins 0.000 claims description 2
108090000623 proteins and genes Proteins 0.000 description 169
102000004169 proteins and genes Human genes 0.000 description 133
235000018102 proteins Nutrition 0.000 description 126
235000001014 amino acid Nutrition 0.000 description 25
230000014509 gene expression Effects 0.000 description 25
239000012634 fragment Substances 0.000 description 24
238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 23
239000000499 gel Substances 0.000 description 18
238000003119 immunoblot Methods 0.000 description 16
208000003495 Coccidiosis Diseases 0.000 description 15
206010023076 Isosporiasis Diseases 0.000 description 15
102000007056 Recombinant Fusion Proteins Human genes 0.000 description 15
108010008281 Recombinant Fusion Proteins Proteins 0.000 description 15
108091028043 Nucleic acid sequence Proteins 0.000 description 14
241000894007 species Species 0.000 description 13
238000012360 testing method Methods 0.000 description 13
108091032973 (ribonucleotides)n+m Proteins 0.000 description 12
108091026890 Coding region Proteins 0.000 description 12
RAXXELZNTBOGNW-UHFFFAOYSA-N imidazole Natural products C1=CNC=N1 RAXXELZNTBOGNW-UHFFFAOYSA-N 0.000 description 12
239000000203 mixture Substances 0.000 description 12
125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 12
239000000047 product Substances 0.000 description 12
210000003936 merozoite Anatomy 0.000 description 11
244000045947 parasite Species 0.000 description 11
238000012163 sequencing technique Methods 0.000 description 11
210000002966 serum Anatomy 0.000 description 11
239000013642 negative control Substances 0.000 description 10
239000000523 sample Substances 0.000 description 10
238000012546 transfer Methods 0.000 description 10
OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 9
101000793655 Oryza sativa subsp. japonica Calreticulin Proteins 0.000 description 9
235000019606 astringent taste Nutrition 0.000 description 9
238000010367 cloning Methods 0.000 description 9
238000011081 inoculation Methods 0.000 description 9
230000001681 protective effect Effects 0.000 description 9
210000003046 sporozoite Anatomy 0.000 description 9
OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 9
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 8
238000007792 addition Methods 0.000 description 8
239000013604 expression vector Substances 0.000 description 8
BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 description 8
108020004999 messenger RNA Proteins 0.000 description 8
230000009257 reactivity Effects 0.000 description 7
238000006467 substitution reaction Methods 0.000 description 7
230000004071 biological effect Effects 0.000 description 6
210000000055 macrogametocyte Anatomy 0.000 description 6
239000012528 membrane Substances 0.000 description 6
238000000746 purification Methods 0.000 description 6
241000196324 Embryophyta Species 0.000 description 5
241000588724 Escherichia coli Species 0.000 description 5
108700026244 Open Reading Frames Proteins 0.000 description 5
238000012217 deletion Methods 0.000 description 5
230000037430 deletion Effects 0.000 description 5
238000002474 experimental method Methods 0.000 description 5
230000005847 immunogenicity Effects 0.000 description 5
238000002955 isolation Methods 0.000 description 5
239000003550 marker Substances 0.000 description 5
244000005700 microbiome Species 0.000 description 5
238000002965 ELISA Methods 0.000 description 4
241001465754 Metazoa Species 0.000 description 4
241000699666 Mus <mouse, genus> Species 0.000 description 4
PXHVJJICTQNCMI-UHFFFAOYSA-N Nickel Chemical compound [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 description 4
239000002033 PVDF binder Substances 0.000 description 4
125000000539 amino acid group Chemical group 0.000 description 4
230000027455 binding Effects 0.000 description 4
230000000740 bleeding effect Effects 0.000 description 4
210000004369 blood Anatomy 0.000 description 4
239000008280 blood Substances 0.000 description 4
239000000872 buffer Substances 0.000 description 4
239000011248 coating agent Substances 0.000 description 4
238000000576 coating method Methods 0.000 description 4
238000011161 development Methods 0.000 description 4
229940079593 drug Drugs 0.000 description 4
239000003814 drug Substances 0.000 description 4
230000000694 effects Effects 0.000 description 4
230000006698 induction Effects 0.000 description 4
239000006166 lysate Substances 0.000 description 4
230000036961 partial effect Effects 0.000 description 4
229920002981 polyvinylidene fluoride Polymers 0.000 description 4
239000011347 resin Substances 0.000 description 4
229920005989 resin Polymers 0.000 description 4
230000001568 sexual effect Effects 0.000 description 4
239000011780 sodium chloride Substances 0.000 description 4
238000010186 staining Methods 0.000 description 4
238000001262 western blot Methods 0.000 description 4
QFVHZQCOUORWEI-UHFFFAOYSA-N 4-[(4-anilino-5-sulfonaphthalen-1-yl)diazenyl]-5-hydroxynaphthalene-2,7-disulfonic acid Chemical compound C=12C(O)=CC(S(O)(=O)=O)=CC2=CC(S(O)(=O)=O)=CC=1N=NC(C1=CC=CC(=C11)S(O)(=O)=O)=CC=C1NC1=CC=CC=C1 QFVHZQCOUORWEI-UHFFFAOYSA-N 0.000 description 3
229920000936 Agarose Polymers 0.000 description 3
102000004190 Enzymes Human genes 0.000 description 3
108090000790 Enzymes Proteins 0.000 description 3
102000003886 Glycoproteins Human genes 0.000 description 3
108090000288 Glycoproteins Proteins 0.000 description 3
FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 3
241000274183 Micromeria Species 0.000 description 3
238000000636 Northern blotting Methods 0.000 description 3
108010033276 Peptide Fragments Proteins 0.000 description 3
102000007079 Peptide Fragments Human genes 0.000 description 3
238000012300 Sequence Analysis Methods 0.000 description 3
238000002105 Southern blotting Methods 0.000 description 3
239000011543 agarose gel Substances 0.000 description 3
230000003321 amplification Effects 0.000 description 3
238000004458 analytical method Methods 0.000 description 3
230000015572 biosynthetic process Effects 0.000 description 3
238000010276 construction Methods 0.000 description 3
230000029087 digestion Effects 0.000 description 3
238000010790 dilution Methods 0.000 description 3
239000012895 dilution Substances 0.000 description 3
238000010828 elution Methods 0.000 description 3
229940088598 enzyme Drugs 0.000 description 3
239000000284 extract Substances 0.000 description 3
230000004927 fusion Effects 0.000 description 3
210000000936 intestine Anatomy 0.000 description 3
238000004949 mass spectrometry Methods 0.000 description 3
239000000463 material Substances 0.000 description 3
229930182817 methionine Natural products 0.000 description 3
238000012986 modification Methods 0.000 description 3
230000004048 modification Effects 0.000 description 3
230000009871 nonspecific binding Effects 0.000 description 3
238000003199 nucleic acid amplification method Methods 0.000 description 3
239000013641 positive control Substances 0.000 description 3
238000000926 separation method Methods 0.000 description 3
239000000243 solution Substances 0.000 description 3
238000005406 washing Methods 0.000 description 3
239000002699 waste material Substances 0.000 description 3
GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 2
206010059866 Drug resistance Diseases 0.000 description 2
ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 2
GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 2
239000006137 Luria-Bertani broth Substances 0.000 description 2
101800000135 N-terminal protein Proteins 0.000 description 2
239000000020 Nitrocellulose Substances 0.000 description 2
108020005187 Oligonucleotide Probes Proteins 0.000 description 2
101800001452 P1 proteinase Proteins 0.000 description 2
108010076504 Protein Sorting Signals Proteins 0.000 description 2
241000700605 Viruses Species 0.000 description 2
239000002671 adjuvant Substances 0.000 description 2
229960000723 ampicillin Drugs 0.000 description 2
AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 2
239000003242 anti bacterial agent Substances 0.000 description 2
229940088710 antibiotic agent Drugs 0.000 description 2
230000000890 antigenic effect Effects 0.000 description 2
238000013459 approach Methods 0.000 description 2
238000003556 assay Methods 0.000 description 2
230000008901 benefit Effects 0.000 description 2
230000008859 change Effects 0.000 description 2
239000003224 coccidiostatic agent Substances 0.000 description 2
239000000356 contaminant Substances 0.000 description 2
238000013461 design Methods 0.000 description 2
230000008030 elimination Effects 0.000 description 2
238000003379 elimination reaction Methods 0.000 description 2
238000000605 extraction Methods 0.000 description 2
239000000706 filtrate Substances 0.000 description 2
239000003999 initiator Substances 0.000 description 2
230000000968 intestinal effect Effects 0.000 description 2
239000008101 lactose Substances 0.000 description 2
210000004962 mammalian cell Anatomy 0.000 description 2
238000005259 measurement Methods 0.000 description 2
230000007246 mechanism Effects 0.000 description 2
210000000052 microgametocyte Anatomy 0.000 description 2
238000010369 molecular cloning Methods 0.000 description 2
229910052759 nickel Inorganic materials 0.000 description 2
229920001220 nitrocellulos Polymers 0.000 description 2
239000002751 oligonucleotide probe Substances 0.000 description 2
230000003071 parasitic effect Effects 0.000 description 2
239000013600 plasmid vector Substances 0.000 description 2
229920002704 polyhistidine Polymers 0.000 description 2
239000002243 precursor Substances 0.000 description 2
230000003252 repetitive effect Effects 0.000 description 2
238000011160 research Methods 0.000 description 2
108091008146 restriction endonucleases Proteins 0.000 description 2
150000003839 salts Chemical class 0.000 description 2
239000003381 stabilizer Substances 0.000 description 2
239000006228 supernatant Substances 0.000 description 2
108020005345 3' Untranslated Regions Proteins 0.000 description 1
108020003589 5' Untranslated Regions Proteins 0.000 description 1
241000224482 Apicomplexa Species 0.000 description 1
208000002109 Argyria Diseases 0.000 description 1
241000238421 Arthropoda Species 0.000 description 1
241000223836 Babesia Species 0.000 description 1
238000009010 Bradford assay Methods 0.000 description 1
UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
101710177832 Co-chaperonin GroES Proteins 0.000 description 1
241000224483 Coccidia Species 0.000 description 1
108020004705 Codon Proteins 0.000 description 1
108020004635 Complementary DNA Proteins 0.000 description 1
238000011537 Coomassie blue staining Methods 0.000 description 1
238000001712 DNA sequencing Methods 0.000 description 1
241000224484 Eimeriidae Species 0.000 description 1
241001342769 Eimeriorina Species 0.000 description 1
241000206602 Eukaryota Species 0.000 description 1
WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
108010003272 Hyaluronate lyase Proteins 0.000 description 1
102000001974 Hyaluronidases Human genes 0.000 description 1
102100034343 Integrase Human genes 0.000 description 1
WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 1
108090001090 Lectins Proteins 0.000 description 1
102000004856 Lectins Human genes 0.000 description 1
101710132682 Lysozyme 1 Proteins 0.000 description 1
241000699670 Mus sp. Species 0.000 description 1
125000001429 N-terminal alpha-amino-acid group Chemical group 0.000 description 1
108091005461 Nucleic proteins Proteins 0.000 description 1
108091034117 Oligonucleotide Proteins 0.000 description 1
108010038807 Oligopeptides Proteins 0.000 description 1
102000015636 Oligopeptides Human genes 0.000 description 1
108091005804 Peptidases Proteins 0.000 description 1
102000035195 Peptidases Human genes 0.000 description 1
241000224016 Plasmodium Species 0.000 description 1
239000002202 Polyethylene glycol Substances 0.000 description 1
108010092799 RNA-directed DNA polymerase Proteins 0.000 description 1
229920002684 Sepharose Polymers 0.000 description 1
BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 1
KEAYESYHFKHZAL-UHFFFAOYSA-N Sodium Chemical compound [Na] KEAYESYHFKHZAL-UHFFFAOYSA-N 0.000 description 1
108010006785 Taq Polymerase Proteins 0.000 description 1
239000007984 Tris EDTA buffer Substances 0.000 description 1
239000007983 Tris buffer Substances 0.000 description 1
241000209140 Triticum Species 0.000 description 1
235000021307 Triticum Nutrition 0.000 description 1
102000004142 Trypsin Human genes 0.000 description 1
108090000631 Trypsin Proteins 0.000 description 1
240000008042 Zea mays Species 0.000 description 1
235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
235000002017 Zea mays subsp mays Nutrition 0.000 description 1
JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 1
230000009471 action Effects 0.000 description 1
238000005349 anion exchange Methods 0.000 description 1
230000000844 anti-bacterial effect Effects 0.000 description 1
230000005784 autoimmunity Effects 0.000 description 1
238000000376 autoradiography Methods 0.000 description 1
201000008680 babesiosis Diseases 0.000 description 1
230000004888 barrier function Effects 0.000 description 1
238000010256 biochemical assay Methods 0.000 description 1
239000008366 buffered solution Substances 0.000 description 1
239000001110 calcium chloride Substances 0.000 description 1
235000011148 calcium chloride Nutrition 0.000 description 1
229910001628 calcium chloride Inorganic materials 0.000 description 1
230000001413 cellular effect Effects 0.000 description 1
238000006243 chemical reaction Methods 0.000 description 1
239000003638 chemical reducing agent Substances 0.000 description 1
230000009194 climbing Effects 0.000 description 1
238000009833 condensation Methods 0.000 description 1
230000005494 condensation Effects 0.000 description 1
238000011109 contamination Methods 0.000 description 1
238000007796 conventional method Methods 0.000 description 1
235000005822 corn Nutrition 0.000 description 1
238000004132 cross linking Methods 0.000 description 1
238000005520 cutting process Methods 0.000 description 1
235000018417 cysteine Nutrition 0.000 description 1
125000000151 cysteine group Chemical class N[C@@H](CS)C(=O)* 0.000 description 1
230000001086 cytosolic effect Effects 0.000 description 1
238000001514 detection method Methods 0.000 description 1
229960000633 dextran sulfate Drugs 0.000 description 1
235000005911 diet Nutrition 0.000 description 1
230000037213 diet Effects 0.000 description 1
201000010099 disease Diseases 0.000 description 1
208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
239000012149 elution buffer Substances 0.000 description 1
239000000839 emulsion Substances 0.000 description 1
238000005516 engineering process Methods 0.000 description 1
210000002919 epithelial cell Anatomy 0.000 description 1
239000003797 essential amino acid Substances 0.000 description 1
235000020776 essential amino acid Nutrition 0.000 description 1
ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 1
229960005542 ethidium bromide Drugs 0.000 description 1
239000004744 fabric Substances 0.000 description 1
230000004720 fertilization Effects 0.000 description 1
210000001035 gastrointestinal tract Anatomy 0.000 description 1
238000001502 gel electrophoresis Methods 0.000 description 1
210000004317 gizzard Anatomy 0.000 description 1
239000008103 glucose Substances 0.000 description 1
239000003292 glue Substances 0.000 description 1
229930195712 glutamate Natural products 0.000 description 1
230000013595 glycosylation Effects 0.000 description 1
238000006206 glycosylation reaction Methods 0.000 description 1
238000000227 grinding Methods 0.000 description 1
230000009931 harmful effect Effects 0.000 description 1
230000036541 health Effects 0.000 description 1
229960002773 hyaluronidase Drugs 0.000 description 1
230000008004 immune attack Effects 0.000 description 1
238000010166 immunofluorescence Methods 0.000 description 1
230000005764 inhibitory process Effects 0.000 description 1
230000000977 initiatory effect Effects 0.000 description 1
238000003780 insertion Methods 0.000 description 1
230000037431 insertion Effects 0.000 description 1
239000002198 insoluble material Substances 0.000 description 1
230000003993 interaction Effects 0.000 description 1
244000000056 intracellular parasite Species 0.000 description 1
230000009545 invasion Effects 0.000 description 1
239000002523 lectin Substances 0.000 description 1
230000003902 lesion Effects 0.000 description 1
230000000670 limiting effect Effects 0.000 description 1
210000004698 lymphocyte Anatomy 0.000 description 1
239000012139 lysis buffer Substances 0.000 description 1
239000002609 medium Substances 0.000 description 1
235000013336 milk Nutrition 0.000 description 1
239000008267 milk Substances 0.000 description 1
210000004080 milk Anatomy 0.000 description 1
LPUQAYUQRXPFSQ-DFWYDOINSA-M monosodium L-glutamate Chemical compound [Na+].[O-]C(=O)[C@@H](N)CCC(O)=O LPUQAYUQRXPFSQ-DFWYDOINSA-M 0.000 description 1
235000013923 monosodium glutamate Nutrition 0.000 description 1
239000004223 monosodium glutamate Substances 0.000 description 1
235000019799 monosodium phosphate Nutrition 0.000 description 1
210000004877 mucosa Anatomy 0.000 description 1
239000002245 particle Substances 0.000 description 1
230000001717 pathogenic effect Effects 0.000 description 1
238000013492 plasmid preparation Methods 0.000 description 1
239000002985 plastic film Substances 0.000 description 1
238000007747 plating Methods 0.000 description 1
229920002401 polyacrylamide Polymers 0.000 description 1
229920001223 polyethylene glycol Polymers 0.000 description 1
238000003752 polymerase chain reaction Methods 0.000 description 1
239000000843 powder Substances 0.000 description 1
238000012545 processing Methods 0.000 description 1
125000001500 prolyl group Chemical group [H]N1C([H])(C(=O)[*])C([H])([H])C([H])([H])C1([H])[H] 0.000 description 1
239000012474 protein marker Substances 0.000 description 1
229940024999 proteolytic enzymes for treatment of wounds and ulcers Drugs 0.000 description 1
230000009467 reduction Effects 0.000 description 1
230000002829 reductive effect Effects 0.000 description 1
230000001105 regulatory effect Effects 0.000 description 1
230000010076 replication Effects 0.000 description 1
238000012216 screening Methods 0.000 description 1
230000011664 signaling Effects 0.000 description 1
229910052709 silver Inorganic materials 0.000 description 1
239000004332 silver Substances 0.000 description 1
238000012868 site-directed mutagenesis technique Methods 0.000 description 1
238000004513 sizing Methods 0.000 description 1
AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 description 1
229910000162 sodium phosphate Inorganic materials 0.000 description 1
239000002689 soil Substances 0.000 description 1
229940031626 subunit vaccine Drugs 0.000 description 1
239000000725 suspension Substances 0.000 description 1
238000013519 translation Methods 0.000 description 1
LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
239000012588 trypsin Substances 0.000 description 1
238000000539 two dimensional gel electrophoresis Methods 0.000 description 1
238000011144 upstream manufacturing Methods 0.000 description 1
238000002255 vaccination Methods 0.000 description 1
239000011534 wash buffer Substances 0.000 description 1
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/44—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from protozoa
- C07K14/455—Eimeria
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P33/00—Antiparasitic agents
- A61P33/02—Antiprotozoals, e.g. for leishmaniasis, trichomoniasis, toxoplasmosis
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
- A61P37/04—Immunostimulants
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies

Landscapes

Health & Medical Sciences (AREA)
Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Life Sciences & Earth Sciences (AREA)
General Health & Medical Sciences (AREA)
Medicinal Chemistry (AREA)
Immunology (AREA)
Veterinary Medicine (AREA)
Public Health (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Pharmacology & Pharmacy (AREA)
General Chemical & Material Sciences (AREA)
Animal Behavior & Ethology (AREA)
Chemical Kinetics & Catalysis (AREA)
Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
Molecular Biology (AREA)
Genetics & Genomics (AREA)
Toxicology (AREA)
Zoology (AREA)
Gastroenterology & Hepatology (AREA)
Biochemistry (AREA)
Biophysics (AREA)
Tropical Medicine & Parasitology (AREA)
Engineering & Computer Science (AREA)
Bioinformatics & Cheminformatics (AREA)
Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Peptides Or Proteins (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

ES02746870T 2001-07-06 2002-07-03 Acidos nucleicos que codifican antigenos recombinantes de 56 y 82 kda de gametocitos de eimeria maxima y sus usos. Expired - Lifetime ES2339227T3 (es)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
US30369901P	2001-07-06	2001-07-06
US303699P		2001-07-06

Publications (1)

Publication Number	Publication Date
ES2339227T3 true ES2339227T3 (es)	2010-05-18

Family

ID=23173289

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
ES02746870T Expired - Lifetime ES2339227T3 (es)	2001-07-06	2002-07-03	Acidos nucleicos que codifican antigenos recombinantes de 56 y 82 kda de gametocitos de eimeria maxima y sus usos.

Country Status (18)

Country	Link
US (2)	US7423137B2 (de)
EP (1)	EP1432722B1 (de)
JP (2)	JP2005501533A (de)
CN (1)	CN1606565B (de)
AT (1)	ATE454392T1 (de)
AU (1)	AU2002316558B2 (de)
BR (1)	BR0210853A (de)
CA (1)	CA2467006A1 (de)
DE (1)	DE60235021D1 (de)
ES (1)	ES2339227T3 (de)
HU (1)	HUP0600028A3 (de)
IL (2)	IL159716A0 (de)
MX (1)	MXPA04000127A (de)
PL (1)	PL366540A1 (de)
RU (1)	RU2004103469A (de)
TR (1)	TR200400049T2 (de)
WO (1)	WO2003004683A2 (de)
ZA (1)	ZA200400479B (de)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
DK0775746T3 (da) *	1995-07-03	2006-04-03	Akzo Nobel Nv	Vaccine mod fjerkræcoccidiose
ZA200400479B (en)	2001-07-06	2006-05-31	Abic Biolog Lab Teva	Nucleic acids encoding recombinant 56 a 82 KDA antigens from gametocytes of Eimeria maxima and their use
CN100348608C (zh)	2001-07-06	2007-11-14	Abic特瓦生物实验室有限公司	编码重组的来源于巨型艾美球虫子孢子/裂殖子的250kDa抗原的核酸,和它们的应用
PL2066339T3 (pl)	2006-09-18	2015-02-27	Univ Arkansas	Kompozycje i sposoby zwiększania odpowiedzi immunologicznych
PT3097926T (pt) *	2007-11-01	2020-01-08	Univ Guelph	Composições e métodos de potenciação das respostas imunitárias frente a eimeria
US20100150958A1 (en) *	2008-12-15	2010-06-17	Vectogen Pty Ltd.	Methods and Compositions for Use of a Coccidiosis Vaccine
AR094791A1 (es)	2013-02-14	2015-08-26	Univ Arkansas	Composiciones y métodos para mejorar las respuestas inmunes ante una infección por eimeria o para limitar una infección por eimeria
AU2015263877B2 (en) *	2014-05-23	2021-01-21	Desert King International Llc	Combination, composition, and method of administering the combination or composition to animals
CN111471107B (zh) *	2020-04-27	2023-04-18	中国农业大学	抗鸡球虫多价重组蛋白卵黄抗体及其制备方法和应用
CN111840529B (zh) *	2020-07-30	2023-08-25	广东省农业科学院动物卫生研究所	一种柔嫩艾美耳球虫重组多肽疫苗vkvq的制备及其在抗鸡球虫病中的应用方法
CN113521264A (zh) *	2021-07-14	2021-10-22	中国农业科学院北京畜牧兽医研究所	一种鸽球虫病活疫苗及其制备方法和应用
CN114051971B (zh) *	2021-08-26	2023-08-29	山东农巧农业科技有限公司	一种高效率乳鸽生态养殖方法
CN114736308B (zh) *	2022-03-15	2022-12-27	四川宜美康科技有限公司	球虫抗原肽/il5的融合蛋白基因工程菌的制备及用途
CN116212009A (zh) *	2022-12-15	2023-06-06	四川农业大学	兔球虫重组蛋白在防控兔球虫病中的应用
CN118360158B (zh) *	2024-05-14	2024-10-29	中国农业科学院上海兽医研究所(中国动物卫生与流行病学中心上海分中心)	柔嫩艾美耳球虫卵囊壁蛋白6（EtOWP6）缺失弱毒株、构建方法、组合物及其应用

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
EP0135712A3 (de)	1983-08-19	1987-05-27	American Cyanamid Company	Gegen Sporozoiten von Eimeria Spp. reaktive monoklonale Antikörper und Antigene
CA1340520C (en)	1984-06-05	1999-05-04	Karel Z. Newman Jr.	Antigenic proteins and vaccines containing them and protective antibodies directed to them for prevention of coccidiosis caused by eimeria tenella and eimeria necatrix
US4639372A (en)	1984-06-29	1987-01-27	Merck & Co., Inc.	Coccidiosis vaccine
WO1990000403A1 (en)	1988-07-05	1990-01-25	Genex Corporation	Genetically engineered coccidiosis vaccine
US5496550A (en) *	1986-08-14	1996-03-05	Chilwalner	Method of reducing the output of Eimeria oocysts from a newborn chick
CA1340838C (en)	1986-08-14	1999-12-07	Michael Wallach	Coccidiosis vacine
ZA893740B (en) *	1988-06-03	1990-02-28	Hoffmann La Roche	Recombinant coccidiosis vaccines
ZA200400479B (en)	2001-07-06	2006-05-31	Abic Biolog Lab Teva	Nucleic acids encoding recombinant 56 a 82 KDA antigens from gametocytes of Eimeria maxima and their use

2001
- 2001-01-22 ZA ZA200400479A patent/ZA200400479B/xx unknown
2002
- 2002-07-03 DE DE60235021T patent/DE60235021D1/de not_active Expired - Lifetime
- 2002-07-03 RU RU2004103469/13A patent/RU2004103469A/ru not_active Application Discontinuation
- 2002-07-03 JP JP2003510441A patent/JP2005501533A/ja active Pending
- 2002-07-03 MX MXPA04000127A patent/MXPA04000127A/es not_active Application Discontinuation
- 2002-07-03 US US10/483,159 patent/US7423137B2/en not_active Expired - Fee Related
- 2002-07-03 BR BR0210853-4A patent/BR0210853A/pt not_active IP Right Cessation
- 2002-07-03 TR TR2004/00049T patent/TR200400049T2/xx unknown
- 2002-07-03 ES ES02746870T patent/ES2339227T3/es not_active Expired - Lifetime
- 2002-07-03 CA CA002467006A patent/CA2467006A1/en not_active Abandoned
- 2002-07-03 WO PCT/US2002/021233 patent/WO2003004683A2/en not_active Ceased
- 2002-07-03 PL PL02366540A patent/PL366540A1/xx not_active Application Discontinuation
- 2002-07-03 IL IL15971602A patent/IL159716A0/xx unknown
- 2002-07-03 EP EP02746870A patent/EP1432722B1/de not_active Expired - Lifetime
- 2002-07-03 HU HU0600028A patent/HUP0600028A3/hu not_active Application Discontinuation
- 2002-07-03 AT AT02746870T patent/ATE454392T1/de not_active IP Right Cessation
- 2002-07-03 CN CN028174976A patent/CN1606565B/zh not_active Expired - Fee Related
- 2002-07-03 AU AU2002316558A patent/AU2002316558B2/en not_active Ceased
2004
- 2004-01-06 IL IL159716A patent/IL159716A/en not_active IP Right Cessation
2008
- 2008-05-30 US US12/156,206 patent/US7968695B2/en not_active Expired - Fee Related
- 2008-09-08 JP JP2008230091A patent/JP2009077713A/ja active Pending

Also Published As

Publication number	Publication date
US20050033042A1 (en)	2005-02-10
EP1432722A2 (de)	2004-06-30
IL159716A0 (en)	2004-06-20
TR200400049T2 (tr)	2009-01-21
HUP0600028A2 (en)	2006-04-28
ZA200400479B (en)	2006-05-31
US20090196888A1 (en)	2009-08-06
BR0210853A (pt)	2005-08-30
AU2002316558B2 (en)	2009-01-08
DE60235021D1 (de)	2010-02-25
EP1432722A4 (de)	2005-08-10
CN1606565B (zh)	2011-06-29
RU2004103469A (ru)	2005-04-10
US7423137B2 (en)	2008-09-09
CA2467006A1 (en)	2003-01-16
IL159716A (en)	2010-06-16
WO2003004683A3 (en)	2004-04-29
HUP0600028A3 (en)	2010-01-28
MXPA04000127A (es)	2005-07-01
JP2005501533A (ja)	2005-01-20
WO2003004683A2 (en)	2003-01-16
EP1432722B1 (de)	2010-01-06
CN1606565A (zh)	2005-04-13
US7968695B2 (en)	2011-06-28
JP2009077713A (ja)	2009-04-16
ATE454392T1 (de)	2010-01-15
PL366540A1 (en)	2005-02-07

Publication	Publication Date	Title
US7968695B2 (en)	2011-06-28	Nucleic acids encoding recombinant 56 and 82 kDa antigens from gametocytes of Eimeria maxima and their uses
US7638131B2 (en)	2009-12-29	Coccidiosis poultry vaccine
MX2011006409A (es)	2011-07-12	Metodos y composiciones para uso de una vacuna de coccidiosis.
JPH0699475B2 (ja)	1994-12-07	コクシジウムのモノクロ−ナル抗体
KR0165115B1 (ko)	1999-02-18	콕시듐증 백신
AU2002316558A1 (en)	2003-05-22	Nucleic acids encoding recombinant 56 and 82 kDa antigens from gametocytes of Eimeria maxima and their uses
KR100244828B1 (ko)	2000-02-15	콕시디아증 백신
Hoan et al.	2016	Identification and immunogenicity of microneme protein 2 (EbMIC2) of Eimeria brunetti
US7462707B1 (en)	2008-12-09	Nucleic acids encoding a recombinant 250 kDa antigen from sporozoites/merozoites of Eimeria maxima and their uses
EP0995799B1 (de)	2007-01-24	Impstoffe gegen Coccidiosis
KR0152245B1 (ko)	1998-10-01	에이메리아 테넬라 왁찐
AU2002316560A1 (en)	2003-05-22	Nucleic acids encoding a recombinant 250 kDa antigen from Sporozoites/Merozoites of Eimeria maxima and their uses
US20110053148A1 (en)	2011-03-03	Coccidiosis vaccines
AU7505198A (en)	1999-01-14	Parasite vaccine