ES2362120T3 - Vector para la selección eficaz y/o maduración de un anticuerpo y usos del mismo. - Google Patents

Vector para la selección eficaz y/o maduración de un anticuerpo y usos del mismo. Download PDF

Info

Publication number
ES2362120T3
ES2362120T3 ES06842807T ES06842807T ES2362120T3 ES 2362120 T3 ES2362120 T3 ES 2362120T3 ES 06842807 T ES06842807 T ES 06842807T ES 06842807 T ES06842807 T ES 06842807T ES 2362120 T3 ES2362120 T3 ES 2362120T3
Authority
ES
Spain
Prior art keywords
antibody
phage
antibodies
image
scfv
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES06842807T
Other languages
English (en)
Spanish (es)
Inventor
Olga Minenkova
Emiliano Pavoni
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sigma Tau Industrie Farmaceutiche Riunite SpA
Tecnogen SpA
Original Assignee
Sigma Tau Industrie Farmaceutiche Riunite SpA
Tecnogen SpA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sigma Tau Industrie Farmaceutiche Riunite SpA, Tecnogen SpA filed Critical Sigma Tau Industrie Farmaceutiche Riunite SpA
Application granted granted Critical
Publication of ES2362120T3 publication Critical patent/ES2362120T3/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/005—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies constructed by phage libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/30—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells
    • C07K16/3007—Carcino-embryonic Antigens
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/30—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells
    • C07K16/3015—Breast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/21—Immunoglobulins specific features characterized by taxonomic origin from primates, e.g. man
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622—Single chain antibody (scFv)

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Oncology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Communicable Diseases (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Indexing, Searching, Synchronizing, And The Amount Of Synchronization Travel Of Record Carriers (AREA)
  • Television Signal Processing For Recording (AREA)
ES06842807T 2005-12-27 2006-12-27 Vector para la selección eficaz y/o maduración de un anticuerpo y usos del mismo. Active ES2362120T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP05028501A EP1803814A1 (fr) 2005-12-27 2005-12-27 Procédé d'amélioration la capacité de sélection d'anticorps dans une bibliothèque d'affichage de phages
EP05028501 2005-12-27

Publications (1)

Publication Number Publication Date
ES2362120T3 true ES2362120T3 (es) 2011-06-28

Family

ID=36177357

Family Applications (1)

Application Number Title Priority Date Filing Date
ES06842807T Active ES2362120T3 (es) 2005-12-27 2006-12-27 Vector para la selección eficaz y/o maduración de un anticuerpo y usos del mismo.

Country Status (17)

Country Link
US (2) US8003383B2 (fr)
EP (2) EP1803814A1 (fr)
JP (1) JP5435956B2 (fr)
AT (1) ATE506435T1 (fr)
AU (1) AU2006329517B2 (fr)
CA (1) CA2634962C (fr)
CY (1) CY1111505T1 (fr)
DE (1) DE602006021476D1 (fr)
DK (1) DK1966375T3 (fr)
ES (1) ES2362120T3 (fr)
HR (1) HRP20110369T1 (fr)
IL (1) IL191633A (fr)
PL (1) PL1966375T3 (fr)
PT (1) PT1966375E (fr)
RS (1) RS51866B (fr)
SI (1) SI1966375T1 (fr)
WO (1) WO2007074496A2 (fr)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1803814A1 (fr) 2005-12-27 2007-07-04 SIGMA-TAU Industrie Farmaceutiche Riunite S.p.A. Procédé d'amélioration la capacité de sélection d'anticorps dans une bibliothèque d'affichage de phages
WO2009109572A2 (fr) * 2008-03-03 2009-09-11 Ablynx Nv Exposition sur phage monovalent de domaines variables simples
WO2013115425A1 (fr) * 2012-01-31 2013-08-08 Scripps Korea Antibody Institute Procédés de criblage d'un anticorps par une technique de panning sur un seul cycle
WO2014096672A1 (fr) 2012-12-17 2014-06-26 Laboratoire Francais Du Fractionnement Et Des Biotechnologies Utilisation d'anticorps monoclonaux pour le traitement de l'inflammation et d'infections bacteriennes
WO2016183222A1 (fr) 2015-05-12 2016-11-17 Regeneron Pharmaceuticals, Inc. Détermination de la pureté de protéine multimère
PL3390441T3 (pl) 2015-12-15 2022-01-10 Gilead Sciences, Inc. Przeciwciała neutralizujące ludzki wirus niedoboru odporności
CA3037694C (fr) 2016-09-30 2023-11-14 Centre National De La Recherche Scientifique Marqueurs cellulaires
TWI728250B (zh) 2017-06-21 2021-05-21 美商基利科學股份有限公司 靶向hiv gp120及cd3之多特異性抗體
CN111196857B (zh) * 2020-02-05 2023-07-21 杭州贤至生物科技有限公司 一种新型冠状病毒多表位重组抗原及其制备方法
CA3177770A1 (fr) * 2020-05-06 2021-11-11 Sorrento Therapeutics, Inc. Anticorps neutralisants se liant a la proteine s de sars-cov-2
WO2022032139A1 (fr) * 2020-08-07 2022-02-10 Sorrento Therapeutics, Inc. Anticorps neutralisants se liant à la protéine s du sars-cov-2
WO2022053188A1 (fr) 2020-09-11 2022-03-17 Alfasigma S.P.A. Nouvelles séquences d'adn encodant des anticorps humains scfv à activité neutralisante contre le sars-coronavirus-2 et leurs utilisations

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3663684A (en) * 1970-06-01 1972-05-16 Hoffmann La Roche Carcinoembryonic antigen and diagnostic method using radioactive iodine
EP0737750B1 (fr) 1989-03-21 2003-05-14 Vical, Inc. Expression de séquences polynucléotidiques exogènes chez un vertébré
GB9015198D0 (en) * 1990-07-10 1990-08-29 Brien Caroline J O Binding substance
JPH0482266A (ja) * 1990-07-24 1992-03-16 Seiko Epson Corp 半導体装置、及びその製造方法
DE69233769D1 (de) * 1991-03-01 2009-09-24 Dyax Corp Chimäres Protein mit Mikroprotein mit zwei oder mehr Disulfidbindungen und Ausgestaltungen davon
PT1696031E (pt) * 1991-12-02 2010-06-25 Medical Res Council Produção de auto-anticorpos a partir de reportórios de segmentos de anticorpo e exibidos em fagos
GB9610967D0 (en) * 1996-05-24 1996-07-31 Cambridge Antibody Tech Specific binding members,materials and methods
US6703015B1 (en) 1999-09-03 2004-03-09 Ramot At Tel-Aviv University Ltd. Filamentous bacteriophage displaying an β-amyloid epitope
AU775679B2 (en) 1998-08-20 2004-08-12 Cell Genesys, Inc. Use of suppressor tRNA's to regulate cytotoxicity during the production of recombinant gene products
CA2286301A1 (fr) 1999-11-01 2001-05-01 Michael Bruce Zwick Bacteriophage filamenteux utilise comme structure multivalente pour le couplage des peptides synthetiques
AU780577B2 (en) * 1999-11-15 2005-04-07 Oncothyreon Inc. Synthetic Lipid-A analogs and uses thereof
TW513852B (en) 2002-01-21 2002-12-11 Nat Chung Cheng Univerity Multiphase oscillator and multiphase oscillation signal generating method
US20040151724A1 (en) * 2002-10-31 2004-08-05 Julia Coronella-Wood Antibody fab fragments specific for breast cancer
EP1789453A2 (fr) 2004-05-18 2007-05-30 Genentech, Inc. Principaux variants de la proteine de coque majeure du virus m13 pour affichage c-terminal et bi-terminal d'une proteine heterologue
EP1803814A1 (fr) 2005-12-27 2007-07-04 SIGMA-TAU Industrie Farmaceutiche Riunite S.p.A. Procédé d'amélioration la capacité de sélection d'anticorps dans une bibliothèque d'affichage de phages
US20130122033A1 (en) 2010-07-02 2013-05-16 Rita De Santis Fimh vaccine against urinary tract infections (uti)

Also Published As

Publication number Publication date
EP1966375B1 (fr) 2011-04-20
RS51866B (sr) 2012-02-29
SI1966375T1 (sl) 2011-07-29
AU2006329517B2 (en) 2012-09-06
US20080286274A1 (en) 2008-11-20
PT1966375E (pt) 2011-06-28
DE602006021476D1 (de) 2011-06-01
WO2007074496A2 (fr) 2007-07-05
DK1966375T3 (da) 2011-06-20
EP1966375A2 (fr) 2008-09-10
CY1111505T1 (el) 2015-08-05
EP1803814A1 (fr) 2007-07-04
USRE45968E1 (en) 2016-04-12
AU2006329517A1 (en) 2007-07-05
ATE506435T1 (de) 2011-05-15
JP2009521241A (ja) 2009-06-04
CA2634962A1 (fr) 2007-07-05
JP5435956B2 (ja) 2014-03-05
IL191633A0 (en) 2008-12-29
HRP20110369T1 (hr) 2011-06-30
PL1966375T3 (pl) 2011-08-31
IL191633A (en) 2012-04-30
US8003383B2 (en) 2011-08-23
WO2007074496A3 (fr) 2008-01-10
CA2634962C (fr) 2015-03-31

Similar Documents

Publication Publication Date Title
Ahmad et al. scFv antibody: principles and clinical application
US7582736B2 (en) Prostate cancer specific internalizing human antibodies
ES2535813T3 (es) Anticuerpos recombinantes y fragmentos que reconocen el gangliósido N-glicolil-GM3 y su uso en el diagnóstico y tratamiento de tumores
US20100240100A1 (en) Human antibodies that have mn binding and cell adhesion-neutralizing activity
KR101740030B1 (ko) ScFv 항체 라이브러리, 이의 제조방법 및 이를 이용한 ScFv 항체 스크리닝 방법
JP2016190839A (ja) 細胞表面の前立腺特異的膜抗原に対するモノクローナル抗体および単鎖抗体フラグメント
CA2386926A1 (fr) Nouveaux composes
IL191633A (en) Vector for efficient selection and/or maturation of an antibody and uses thereof
WO2004007717A1 (fr) Criblage proteomique pour identifier des molecules biologiques associees a des maladies et leurs inhibiteurs
HUP0400775A2 (en) Specific human antibodies for selective cancer therapy
Ayat et al. Isolation of scFv antibody fragments against HER2 and CEA tumor antigens from combinatorial antibody libraries derived from cancer patients
KR102698752B1 (ko) 암 치료법에서 괴사를 표적화하기 위한 인간화 항-핵 항체
Wong et al. Human scFv antibody fragments specific for the epithelial tumour marker MUC-1, selected by phage display on living cells
KR20100064985A (ko) 인간 및 마우스에서 발현하는 ｌ1ｃａｍ에 특이적으로 결합하는 재조합 단일클론항체 및 그 이용
AU775876B2 (en) Novel antibody with specificity for colon cancer
Bao et al. Isolating human antibody against human hepatocellular carcinoma by guided-selection
Spillner et al. Paratope-based protein identification by antibody and peptide phage display
CN121194995A (zh) 抗bcam抗体及其抗体-药物偶联物
AU2002349969A1 (en) Human antibodies that have MN binding and cell adhesion-neutralizing activity