ES2409537B8 - Medio de cultivo celular útil para la proliferación in vitro de células del epitelio del limbo esclerocorneal - Google Patents

Medio de cultivo celular útil para la proliferación in vitro de células del epitelio del limbo esclerocorneal Download PDF

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Publication number
ES2409537B8
ES2409537B8 ES201132081A ES201132081A ES2409537B8 ES 2409537 B8 ES2409537 B8 ES 2409537B8 ES 201132081 A ES201132081 A ES 201132081A ES 201132081 A ES201132081 A ES 201132081A ES 2409537 B8 ES2409537 B8 ES 2409537B8
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Prior art keywords
cells
cell culture
scalcorneal
epitel
limb
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Active
Application number
ES201132081A
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English (en)
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ES2409537B1 (es
ES2409537A1 (es
Inventor
Margarita Calonge Cano
Rosa María CORRALES HERRÁN
Marina LÓPEZ PANIAGUA
Ana DE LA MATA SAMPEDRO
Sara GALINDO DE LA ROSA
Teresa NIETO MIGUEL
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Universidad de Valladolid
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Universidad de Valladolid
Centro de Investigacion Biomedica en Red en Bioingenieria Biomateriales y Nanomedicina CIBERBBN
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Priority to ES201132081A priority Critical patent/ES2409537B8/es
Publication of ES2409537A1 publication Critical patent/ES2409537A1/es
Publication of ES2409537B1 publication Critical patent/ES2409537B1/es
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/13—Amines
    • A61K31/135—Amines having aromatic rings, e.g. ketamine, nortriptyline
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Cell Biology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Epidemiology (AREA)
  • Dermatology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Veterinary Medicine (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Public Health (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

Medio de cultivo celular útil para la proliferación in vitro de células de epitelio del limbo esclerocorneal.#La presente invención proporciona un medio de cultivo celular carente de componentes de origen animal no humano y de compuestos nocivos para el organismo humano, útil para la proliferación in vitro de células de epitelio del limbo esclerocorneal, tanto de las células epiteliales diferenciadas o en proceso de diferenciación como de las células madre presentes entre ellas. Por tanto, las células cultivadas en el medo de cultivo de la invención pueden ser aplicadas de forma segura en clínica, por ejemplo, en procedimientos de terapia celular para la regeneración epitelial de la superficie ocular, preferiblemente en los casos de Síndrome de Insuficiencia Límbica (SIL).
ES201132081A 2011-12-22 2011-12-22 Medio de cultivo celular útil para la proliferación in vitro de células del epitelio del limbo esclerocorneal Active ES2409537B8 (es)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES201132081A ES2409537B8 (es) 2011-12-22 2011-12-22 Medio de cultivo celular útil para la proliferación in vitro de células del epitelio del limbo esclerocorneal

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES201132081A ES2409537B8 (es) 2011-12-22 2011-12-22 Medio de cultivo celular útil para la proliferación in vitro de células del epitelio del limbo esclerocorneal

Publications (3)

Publication Number Publication Date
ES2409537A1 ES2409537A1 (es) 2013-06-26
ES2409537B1 ES2409537B1 (es) 2014-04-30
ES2409537B8 true ES2409537B8 (es) 2014-10-09

Family

ID=48577339

Family Applications (1)

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ES201132081A Active ES2409537B8 (es) 2011-12-22 2011-12-22 Medio de cultivo celular útil para la proliferación in vitro de células del epitelio del limbo esclerocorneal

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Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018111908A1 (en) * 2016-12-13 2018-06-21 The Regents Of The University Of California Xenobiotic-free culture system to expand human limbal stem cells

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6692961B1 (en) * 1996-10-11 2004-02-17 Invitrogen Corporation Defined systems for epithelial cell culture and use thereof
CN101347640A (zh) * 2008-09-05 2009-01-21 西安交通大学 用于筛选退色剂的色素化皮肤类似物模型及其构建方法
CN101486994B (zh) * 2009-02-24 2011-01-05 中国检验检疫科学研究院 促进组织工程皮肤脂质结构形成的培养液及其配制方法

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Publication number Publication date
ES2409537B1 (es) 2014-04-30
ES2409537A1 (es) 2013-06-26

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