ES2443047T3 - Método para mejorar la actividad enzimática - Google Patents

Método para mejorar la actividad enzimática Download PDF

Info

Publication number: ES2443047T3
Authority: ES; Spain
Prior art keywords: polynucleotides; polynucleotide; solid; solid phases; variants
Prior art date: 2008-04-04
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Active

Application number

ES09727374.2T

Other languages

English (en)

Spanish (es)

Inventor

Robert G. Blazej

Brian M. Paegel

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Novozymes AS

Scripps Research Institute

Allopartis Biotechnologies Inc

Original Assignee

Novozymes AS

Scripps Research Institute

Allopartis Biotechnologies Inc

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2008-04-04

Filing date

2009-04-03

Publication date

2014-02-17

2009-04-03 Application filed by Novozymes AS, Scripps Research Institute, Allopartis Biotechnologies Inc filed Critical Novozymes AS

2014-02-17 Application granted granted Critical

2014-02-17 Publication of ES2443047T3 publication Critical patent/ES2443047T3/es

Status Active legal-status Critical Current

2029-04-03 Anticipated expiration legal-status Critical

Links

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Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1075—Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

Life Sciences & Earth Sciences (AREA)
Genetics & Genomics (AREA)
Health & Medical Sciences (AREA)
Chemical & Material Sciences (AREA)
Engineering & Computer Science (AREA)
Bioinformatics & Cheminformatics (AREA)
Organic Chemistry (AREA)
Zoology (AREA)
Biomedical Technology (AREA)
Wood Science & Technology (AREA)
Biotechnology (AREA)
General Engineering & Computer Science (AREA)
Crystallography & Structural Chemistry (AREA)
Plant Pathology (AREA)
Molecular Biology (AREA)
Microbiology (AREA)
Biophysics (AREA)
Physics & Mathematics (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
Bioinformatics & Computational Biology (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Enzymes And Modification Thereof (AREA)

ES09727374.2T 2008-04-04 2009-04-03 Método para mejorar la actividad enzimática Active ES2443047T3 (es)

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US4263708P	2008-04-04	2008-04-04
US42637P		2008-04-04
PCT/US2009/039558 WO2009124296A2 (en)	2008-04-04	2009-04-03	Method of enhancing enzyme activity

Publications (1)

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US (2)	US8557742B2 (pt)
EP (1)	EP2260130B1 (pt)
AU (1)	AU2009231585B2 (pt)
BR (1)	BRPI0911326B1 (pt)
CA (1)	CA2719211C (pt)
DK (1)	DK2260130T3 (pt)
ES (1)	ES2443047T3 (pt)
MX (1)	MX2010010798A (pt)
WO (1)	WO2009124296A2 (pt)

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CA2719211C (en)	2008-04-04	2017-01-24	Allopartis Biotechnologies, Inc.	Method of enhancing enzyme activity
WO2016164583A2 (en)	2015-04-07	2016-10-13	Novozymes A/S	Methods for selecting enzymes having enhanced activity
US20180355404A1 (en) *	2015-04-07	2018-12-13	Novozymes A/S	Methods for selecting enzymes having lipase activity
WO2016196691A2 (en)	2015-06-01	2016-12-08	California Institute Of Technology	Compositions and methods for screening t cells with antigens for specific populations
US20190161786A1 (en)	2016-04-07	2019-05-30	Novozymes A/S	Methods for selecting enzymes having protease activity
US12258613B2 (en)	2017-03-08	2025-03-25	California Institute Of Technology	Pairing antigen specificity of a T cell with T cell receptor sequences
GB2569561A (en) *	2017-12-19	2019-06-26	Sphere Fluidics Ltd	Methods for performing biological reactions
WO2020223675A1 (en)	2019-05-02	2020-11-05	The Regents Of The University Of California	Laser-induced confocal microscope and microfluidic device for dielectrophoretic fluorescence-activated droplet sorting
AU2020361681A1 (en)	2019-10-10	2022-05-05	1859, Inc.	Methods and systems for microfluidic screening

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GB2342094B (en)	1997-07-07	2002-10-23	Medical Res Council	In vitro sorting method
WO2003040342A2 (en) *	2001-11-07	2003-05-15	Integrigen, Inc.	Method for generation of modular polynucleotides using solid supports
CN101128601B (zh) *	2003-01-29	2011-06-08	454生命科学公司	核酸扩增和测序的方法
US20050106577A1 (en) *	2003-11-17	2005-05-19	Hashem Akhavan-Tafti	Cleavable solid phases for isolating nucleic acids
EP1691792A4 (en) *	2003-11-24	2008-05-28	Yeda Res & Dev	COMPOSITIONS AND PROCESSES FOR SORTING IN VITRO OF MOLECULE AND CELL LIBRARIES
EA018577B1 (ru) *	2005-03-15	2013-09-30	Верениум Корпорейшн	Целлюлазы, нуклеиновые кислоты, кодирующие их, и способы их получения и применения
US7785854B2 (en) *	2006-08-31	2010-08-31	Iogen Energy Corporation	Modified cellulases with increased thermostability, thermophilicity, and alkalophilicity
CA2719211C (en)	2008-04-04	2017-01-24	Allopartis Biotechnologies, Inc.	Method of enhancing enzyme activity

2009
- 2009-04-03 CA CA2719211A patent/CA2719211C/en active Active
- 2009-04-03 WO PCT/US2009/039558 patent/WO2009124296A2/en not_active Ceased
- 2009-04-03 EP EP09727374.2A patent/EP2260130B1/en active Active
- 2009-04-03 US US12/935,249 patent/US8557742B2/en not_active Expired - Fee Related
- 2009-04-03 AU AU2009231585A patent/AU2009231585B2/en not_active Ceased
- 2009-04-03 MX MX2010010798A patent/MX2010010798A/es active IP Right Grant
- 2009-04-03 ES ES09727374.2T patent/ES2443047T3/es active Active
- 2009-04-03 BR BRPI0911326 patent/BRPI0911326B1/pt not_active IP Right Cessation
- 2009-04-03 DK DK09727374.2T patent/DK2260130T3/da active
2013
- 2013-10-14 US US14/053,439 patent/US20140045723A1/en not_active Abandoned

Also Published As

Publication number	Publication date
AU2009231585A1 (en)	2009-10-08
CA2719211A1 (en)	2009-10-08
US20110046012A1 (en)	2011-02-24
WO2009124296A2 (en)	2009-10-08
BRPI0911326A2 (pt)	2016-10-11
US8557742B2 (en)	2013-10-15
AU2009231585B2 (en)	2014-08-14
DK2260130T3 (da)	2014-01-27
EP2260130B1 (en)	2013-10-23
US20140045723A1 (en)	2014-02-13
EP2260130A2 (en)	2010-12-15
BRPI0911326B1 (pt)	2019-11-26
MX2010010798A (es)	2011-01-21
WO2009124296A3 (en)	2009-12-30
CA2719211C (en)	2017-01-24
EP2260130A4 (en)	2012-04-04

Publication	Publication Date	Title
ES2443047T3 (es)	2014-02-17	Método para mejorar la actividad enzimática
Taly et al.	2007	Droplets as microreactors for high‐throughput biology
ES2392990T3 (es)	2012-12-17	Método de clasificación óptica
ES2274901T3 (es)	2007-06-01	Procedimiento de evolucion dirigida.
ES2303959T3 (es)	2008-09-01	Cst (automarcado compartimentalizado).
AU2002304450B2 (en)	2008-05-08	Selective gene amplification
US9701959B2 (en)	2017-07-11	High throughput screen for biologically active polypeptides
JP5997691B2 (ja)	2016-09-28	酵素
US20060003347A1 (en)	2006-01-05	Single-molecule in vitro evolution
ES2404665T3 (es)	2013-05-28	Polimerasas de ADN que incorporan análogos de nucleótidos marcados con colorante
AU2002304450A1 (en)	2003-05-15	Selective gene amplification
US20080004436A1 (en)	2008-01-03	Directed Evolution and Selection Using in Vitro Compartmentalization
US12116570B2 (en)	2024-10-15	Directed evolution of enzymes by plasmid tagging in droplets
Chetverin et al.	2007	Scientific and practical applications of molecular colonies
Gielen	2021	Single-Cell Microencapsulation for Evolution and Discovery of Biocatalysts
Barendt et al.	2009	Cell-free display systems for protein engineering
Wang et al.	2019	A TNA enzyme capable of discriminating single point mutation
Hollfelder	0	Evolution of enzyme catalysts caged in biomimetic gel-shell beads
Patil	2014	Evolution of enzyme catalysts caged in biomimetic gel-shell beads