ES2530979T3 - Detección de ácidos nucleicos y proteínas - Google Patents

Detección de ácidos nucleicos y proteínas Download PDF

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Publication number
ES2530979T3
ES2530979T3 ES11798559T ES11798559T ES2530979T3 ES 2530979 T3 ES2530979 T3 ES 2530979T3 ES 11798559 T ES11798559 T ES 11798559T ES 11798559 T ES11798559 T ES 11798559T ES 2530979 T3 ES2530979 T3 ES 2530979T3
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ES
Spain
Prior art keywords
aptamer
nucleic acid
sample
probe
detecting
Prior art date
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Active
Application number
ES11798559T
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English (en)
Inventor
Anthony P Shuber
Eugene Chiu
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PHYSICIANS CHOICE LAB SERVICES LLC
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PHYSICIANS CHOICE LAB SERVICES LLC
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/575—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/60—Complex ways of combining multiple protein biomarkers for diagnosis

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)

Abstract

Un método para detectar múltiples analitos en una muestra, comprendiendo dicho método las etapas de: obtener una muestra que comprende al menos un ácido nucleico diana y al menos una proteína diana; introducir un aptámero que se une a la proteína diana en la muestra; y realizar un único ensayo, en el que el ensayo detecta el ácido nucleico diana y la proteína diana, comprendiendo dicho ensayo las etapas de: retirar el aptámero no unido del complejo de aptámero-proteína; realizar una PCR con el ácido nucleico diana; realizar una PCR con el aptámero; introducir una primera sonda marcada de modo detectable que se une al ácido nucleico diana amplificado; introducir una segunda sonda marcada de modo detectable que se une al aptámero amplificado; detectar la primera sonda, detectando con ello el ácido nucleico en la muestra; y detectar la segunda sonda que está unida al aptámero amplificado, en el que la detección de la segunda sonda detecta la proteína diana.
ES11798559T 2010-06-21 2011-05-23 Detección de ácidos nucleicos y proteínas Active ES2530979T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US12/819,700 US7955822B2 (en) 2007-09-14 2010-06-21 Detection of nucleic acids and proteins
PCT/US2011/037539 WO2011162894A1 (en) 2010-06-21 2011-05-23 Detection of nucleic acids and proteins

Publications (1)

Publication Number Publication Date
ES2530979T3 true ES2530979T3 (es) 2015-03-09

Family

ID=45371752

Family Applications (1)

Application Number Title Priority Date Filing Date
ES11798559T Active ES2530979T3 (es) 2010-06-21 2011-05-23 Detección de ácidos nucleicos y proteínas

Country Status (5)

Country Link
US (3) US7955822B2 (es)
EP (1) EP2582849B1 (es)
CA (1) CA2801305A1 (es)
ES (1) ES2530979T3 (es)
WO (1) WO2011162894A1 (es)

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US20120252019A1 (en) * 2007-08-17 2012-10-04 Predictive Biosciences, Inc. Detection of Bladder Cancers
US20120252020A1 (en) * 2007-08-17 2012-10-04 Predictive Biosciences, Inc. Screening Assay for Bladder Cancer
US7955822B2 (en) * 2007-09-14 2011-06-07 Predictive Biosciences Corp. Detection of nucleic acids and proteins
US20130203058A1 (en) * 2012-02-02 2013-08-08 Anthony P. Shuber Composite assay for detecting a clinical condition
CN103163235B (zh) * 2012-07-12 2014-09-24 海南合瑞制药股份有限公司 固相合成胸腺肽α1的16肽阶段的缺失肽及检测方法
CN103163236B (zh) * 2012-07-12 2014-09-24 海南合瑞制药股份有限公司 鉴别纯化后的胸腺肽α1是否含有缺失肽的方法
CN103163234B (zh) * 2012-07-12 2014-09-24 海南合瑞制药股份有限公司 固相合成胸腺肽α1的11肽阶段的缺失肽、非肽杂质及检测方法
CN106170558B (zh) 2013-10-21 2021-10-29 金圣千 利用寡核苷酸的生物分子的分析方法及装置
CN107614685B (zh) * 2015-04-17 2021-10-19 肯塔基大学研究基金会 Rna纳米颗粒及其使用方法
KR101801227B1 (ko) 2016-01-14 2017-11-24 고려대학교 산학협력단 폴리스타틴에 특이적으로 결합하는 핵산 앱타머 및 그 용도
WO2023060267A1 (en) * 2021-10-07 2023-04-13 Serimmune Inc. Global protein-based immunome wide association studies

Family Cites Families (19)

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US5324634A (en) * 1992-03-31 1994-06-28 The Research Foundation Of State University Of New York Diagnostic tests measuring gelatinase/inhibitor complexes for detection of aggressive and metastatic cancer
US6566101B1 (en) * 1997-06-16 2003-05-20 Anthony P. Shuber Primer extension methods for detecting nucleic acids
AU2002322348A1 (en) * 2001-06-28 2003-03-03 Advanced Research And Technology Institute, Inc. Methods of preparing multicolor quantum dot tagged beads and conjugates thereof
US20040219509A1 (en) * 2001-08-20 2004-11-04 Biosite, Inc. Diagnostic markers of stroke and cerebral injury and methods of use thereof
WO2004059293A2 (en) 2002-12-24 2004-07-15 Biosite Incorporated Markers for differential diagnosis and methods of use thereof
US7666583B2 (en) * 2004-02-19 2010-02-23 Yale University Identification of cancer protein biomarkers using proteomic techniques
CA2605143A1 (en) * 2005-04-15 2006-10-26 Becton, Dickinson And Company Diagnosis of sepsis
US20070099239A1 (en) * 2005-06-24 2007-05-03 Raymond Tabibiazar Methods and compositions for diagnosis and monitoring of atherosclerotic cardiovascular disease
WO2007050706A2 (en) * 2005-10-27 2007-05-03 University Of Missouri-Columbia Dna methylation biomarkers in lymphoid and hematopoietic malignancies
US20070122830A1 (en) * 2005-11-10 2007-05-31 Aurelium Biopharma Inc. Prohibitin-directed diagnostics and therapeutics for cancer and chemotherapeutic drug resistance
EP1969363B1 (en) * 2005-12-22 2016-11-30 Abbott Molecular Inc. Methods and marker combinations for screening for predisposition to lung cancer
US20070255113A1 (en) * 2006-05-01 2007-11-01 Grimes F R Methods and apparatus for identifying disease status using biomarkers
WO2008105814A2 (en) * 2006-08-22 2008-09-04 Los Alamos National Security, Llc Miniturized lateral flow device for rapid and sensitive detection of proteins or nucleic acids
CA2679205A1 (en) 2007-02-23 2008-08-28 Predictive Biosciences Corporation Clinical intervention directed diagnostic methods
US20090047694A1 (en) * 2007-08-17 2009-02-19 Shuber Anthony P Clinical Intervention Directed Diagnostic Methods
KR100896987B1 (ko) * 2007-03-14 2009-05-14 한국과학기술연구원 앱타머를 이용한 표적 단백질 검출 방법 및 검출키트
US8058415B2 (en) * 2007-04-24 2011-11-15 The Board Of Trustees Of The University Of Illinois Aptamer- and nucleic acid enzyme-based systems for simultaneous detection of multiple analytes
US20090075266A1 (en) * 2007-09-14 2009-03-19 Predictive Biosciences Corporation Multiple analyte diagnostic readout
US7955822B2 (en) * 2007-09-14 2011-06-07 Predictive Biosciences Corp. Detection of nucleic acids and proteins

Also Published As

Publication number Publication date
WO2011162894A1 (en) 2011-12-29
US8530198B2 (en) 2013-09-10
US20130316356A1 (en) 2013-11-28
EP2582849A4 (en) 2013-12-04
EP2582849B1 (en) 2015-01-07
US20110129837A1 (en) 2011-06-02
US9080206B2 (en) 2015-07-14
US20110014618A1 (en) 2011-01-20
US7955822B2 (en) 2011-06-07
CA2801305A1 (en) 2011-12-29
EP2582849A1 (en) 2013-04-24

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