ES2531055T3 - Cebadores de polinucleótidos para detectar mutaciones de PIK3CA - Google Patents

Cebadores de polinucleótidos para detectar mutaciones de PIK3CA Download PDF

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Publication number
ES2531055T3
ES2531055T3 ES12169604T ES12169604T ES2531055T3 ES 2531055 T3 ES2531055 T3 ES 2531055T3 ES 12169604 T ES12169604 T ES 12169604T ES 12169604 T ES12169604 T ES 12169604T ES 2531055 T3 ES2531055 T3 ES 2531055T3
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polynucleotide
primer
polynucleotide primers
pik3ca mutations
detect
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Ruth Board
Jennifer Ferguson
Paul Francis Ravetto
Nicola Thelwell
David Whitcombe
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Qiagen Manchester Ltd
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Qiagen Manchester Ltd
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
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  • Wood Science & Technology (AREA)
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  • Analytical Chemistry (AREA)
  • Immunology (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
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  • General Engineering & Computer Science (AREA)
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  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
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  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Un método de PCR para detectar la presencia o la ausencia de una mutación en el gen PIK3CA que comprende las etapas de: a) mezclar una muestra de ácido nucleico que comprende al menos un fragmento del gen PIK3CA con una secuencia de polinucleótido cebador que consiste en la SEQ ID NO. 28, donde el polinucleótido se usa como cebador y el segundo cebador corresponde a una región del fragmento de la secuencia de PIK3CA por debajo de la región a la cual el polinucleótido es complementario, y llevar a cabo una PCR con la mezcla; y b) detectar la hibridación del polinucleótido con la muestra de ácido nucleico, donde la hibridación indica la presencia de una mutación.
ES12169604T 2007-09-28 2008-09-29 Cebadores de polinucleótidos para detectar mutaciones de PIK3CA Active ES2531055T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB0719034A GB2453173A (en) 2007-09-28 2007-09-28 Polynucleotide primers

Publications (1)

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ES2531055T3 true ES2531055T3 (es) 2015-03-10

Family

ID=38701889

Family Applications (6)

Application Number Title Priority Date Filing Date
ES10158519.8T Active ES2532141T3 (es) 2007-09-28 2008-09-29 Cebadores de polinucleótidos para detectar mutaciones de PIK3CA
ES12169586.0T Active ES2622885T3 (es) 2007-09-28 2008-09-29 Cebadores polinucleótidos para detectar mutaciones PIK3CA
ES10158523.0T Active ES2531994T3 (es) 2007-09-28 2008-09-29 Cebadores de polinucleótidos para detectar mutaciones de PIK3CA
ES12169604T Active ES2531055T3 (es) 2007-09-28 2008-09-29 Cebadores de polinucleótidos para detectar mutaciones de PIK3CA
ES12169599.3T Active ES2620289T3 (es) 2007-09-28 2008-09-29 Cebadores polinucleótidos para detectar mutaciones PIK3CA
ES12169594.4T Active ES2532225T3 (es) 2007-09-28 2008-09-29 Cebadores de polinucleótidos para detectar mutaciones de PIK3CA

Family Applications Before (3)

Application Number Title Priority Date Filing Date
ES10158519.8T Active ES2532141T3 (es) 2007-09-28 2008-09-29 Cebadores de polinucleótidos para detectar mutaciones de PIK3CA
ES12169586.0T Active ES2622885T3 (es) 2007-09-28 2008-09-29 Cebadores polinucleótidos para detectar mutaciones PIK3CA
ES10158523.0T Active ES2531994T3 (es) 2007-09-28 2008-09-29 Cebadores de polinucleótidos para detectar mutaciones de PIK3CA

Family Applications After (2)

Application Number Title Priority Date Filing Date
ES12169599.3T Active ES2620289T3 (es) 2007-09-28 2008-09-29 Cebadores polinucleótidos para detectar mutaciones PIK3CA
ES12169594.4T Active ES2532225T3 (es) 2007-09-28 2008-09-29 Cebadores de polinucleótidos para detectar mutaciones de PIK3CA

Country Status (13)

Country Link
US (4) US8901285B2 (es)
EP (7) EP2505673B1 (es)
JP (1) JP5719593B2 (es)
KR (4) KR101543215B1 (es)
CN (4) CN102119222B (es)
AU (1) AU2008303400B2 (es)
BR (1) BRPI0817444A2 (es)
CA (2) CA2700710C (es)
ES (6) ES2532141T3 (es)
GB (1) GB2453173A (es)
MX (1) MX2010003486A (es)
RU (1) RU2491289C2 (es)
WO (1) WO2009040557A2 (es)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10190171B2 (en) 2007-09-28 2019-01-29 Qiagen Manchester Limited Polynucleotide primers for detecting PIK3CA mutations

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CA2786696C (en) 2010-01-12 2021-05-25 Jill Detmer Oligonucleotides and methods for detecting pik3ca mutations
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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10190171B2 (en) 2007-09-28 2019-01-29 Qiagen Manchester Limited Polynucleotide primers for detecting PIK3CA mutations
US10196692B2 (en) 2007-09-28 2019-02-05 Qiagen Manchester Limited Polynucleotide primers for detecting PIK3CA mutations

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GB0719034D0 (en) 2007-11-07
MX2010003486A (es) 2010-08-09
US20160032408A1 (en) 2016-02-04
KR101543215B1 (ko) 2015-08-07
US20110027779A1 (en) 2011-02-03
CN103695558B (zh) 2016-01-20
EP2505671A1 (en) 2012-10-03
RU2010116772A (ru) 2011-11-10
EP2236627A8 (en) 2010-12-01
EP2505673A1 (en) 2012-10-03
CN102119222A (zh) 2011-07-06
US9863003B2 (en) 2018-01-09
EP2508624A1 (en) 2012-10-10
KR20150038660A (ko) 2015-04-08
CN103952466B (zh) 2016-07-06
KR20100101070A (ko) 2010-09-16
WO2009040557A2 (en) 2009-04-02
US8901285B2 (en) 2014-12-02
US10190171B2 (en) 2019-01-29
KR101543216B1 (ko) 2015-08-07
EP2239341A8 (en) 2010-12-01
EP2239341A1 (en) 2010-10-13
EP2205760A2 (en) 2010-07-14
CA3010064A1 (en) 2009-04-02
EP2508624B1 (en) 2015-01-14
EP2239341B1 (en) 2015-01-14
EP2236627A1 (en) 2010-10-06
ES2532141T3 (es) 2015-03-24
KR20150038661A (ko) 2015-04-08
US20140141425A1 (en) 2014-05-22
EP2505672A1 (en) 2012-10-03
ES2532225T3 (es) 2015-03-25
AU2008303400A1 (en) 2009-04-02
EP2505673B1 (en) 2017-02-01
AU2008303400B2 (en) 2013-09-26
US10196692B2 (en) 2019-02-05
RU2491289C2 (ru) 2013-08-27
CA2700710A1 (en) 2009-04-02
EP2205760B1 (en) 2012-06-27
JP2010539920A (ja) 2010-12-24
JP5719593B2 (ja) 2015-05-20
US20160040255A1 (en) 2016-02-11
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KR101548758B1 (ko) 2015-09-01
WO2009040557A3 (en) 2009-05-14
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ES2622885T3 (es) 2017-07-07
CN103834724B (zh) 2016-05-25
CN102119222B (zh) 2015-11-25
EP2505672B1 (en) 2015-01-14
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