ES2551029T3 - Alpha-amylase variants - Google Patents
Alpha-amylase variants Download PDFInfo
- Publication number
- ES2551029T3 ES2551029T3 ES06110159.8T ES06110159T ES2551029T3 ES 2551029 T3 ES2551029 T3 ES 2551029T3 ES 06110159 T ES06110159 T ES 06110159T ES 2551029 T3 ES2551029 T3 ES 2551029T3
- Authority
- ES
- Spain
- Prior art keywords
- alpha
- amylase
- mother
- termamyl
- variant
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 108090000637 alpha-Amylases Proteins 0.000 title abstract 4
- 102000004139 alpha-Amylases Human genes 0.000 title abstract 4
- 229940024171 alpha-amylase Drugs 0.000 title abstract 4
- 108010075550 termamyl Proteins 0.000 abstract 2
- 239000004382 Amylase Substances 0.000 abstract 1
- 108010065511 Amylases Proteins 0.000 abstract 1
- 102000013142 Amylases Human genes 0.000 abstract 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 abstract 1
- 235000019418 amylase Nutrition 0.000 abstract 1
- 229910052791 calcium Inorganic materials 0.000 abstract 1
- 239000011575 calcium Substances 0.000 abstract 1
- 238000006467 substitution reaction Methods 0.000 abstract 1
Landscapes
- Enzymes And Modification Thereof (AREA)
Abstract
Variante de una alfa-amilasa madre de tipo Termamyl, donde (a) la variante comprende una o varias de las siguientes sustituciones: E376K; S417T; A420Q o R; S356A; o Y358F y además K176R+I201F+ H205N utilizando la numeración en SEC ID nº: 4, donde la alfa-amilasa madre de tipo Termamyl tiene un grado de identidad para SEC ID nº: 4 de al menos 80%, y (b) la variante tiene actividad de alfa-amilasa y además las variantes tienen y una estabilidad aumentada a pH por debajo de 7.0, y/o a concentraciones de calcio por debajo de 1 mM a temperaturas en el rango de 95°C a 160°C relativamente a la alfa-amilasa madre de tipo Termamyl.Variant of a mother alpha-amylase of the Termamyl type, where (a) the variant comprises one or more of the following substitutions: E376K; S417T; A420Q or R; S356A; or Y358F and in addition K176R + I201F + H205N using the numbering in SEQ ID NO: 4, where the mother alpha-amylase of the Termamyl type has an identity degree for SEQ ID NO: 4 of at least 80%, and (b) the variant It has alpha-amylase activity and in addition the variants have and an increased stability at pH below 7.0, and / or calcium concentrations below 1 mM at temperatures in the range of 95 ° C to 160 ° C relative to the alpha Termamyl-type mother amylase.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DKPA199801495 | 1998-11-16 | ||
| DK149598 | 1998-11-16 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2551029T3 true ES2551029T3 (en) | 2015-11-13 |
Family
ID=54398561
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES06110159.8T Expired - Lifetime ES2551029T3 (en) | 1998-11-16 | 1999-11-16 | Alpha-amylase variants |
Country Status (1)
| Country | Link |
|---|---|
| ES (1) | ES2551029T3 (en) |
-
1999
- 1999-11-16 ES ES06110159.8T patent/ES2551029T3/en not_active Expired - Lifetime
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