ES2565560B2 - Adaptive Thermocycler - Google Patents

Adaptive Thermocycler Download PDF

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Publication number
ES2565560B2
ES2565560B2 ES201500578A ES201500578A ES2565560B2 ES 2565560 B2 ES2565560 B2 ES 2565560B2 ES 201500578 A ES201500578 A ES 201500578A ES 201500578 A ES201500578 A ES 201500578A ES 2565560 B2 ES2565560 B2 ES 2565560B2
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peltier
insulator
cycle exchanger
rough surface
insulating layers
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ES2565560A1 (en
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Ángel Gutiérrez Miguélez
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Universidad de Alicante
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Universidad de Alicante
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    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00Heating or cooling apparatus; Heat insulating devices
    • B01L7/52Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00Heating or cooling apparatus; Heat insulating devices

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Termociclador adaptable.#Consistente en un sistema programable que permite cambiar la temperatura del contenido de un volumen variable un número de veces entre unas temperaturas preestablecidas sin límite de potencias, y con un sensor que permite saber la temperatura en dicho volumen.Adaptive thermocycler. # Consisting of a programmable system that allows changing the temperature of the content of a variable volume a number of times between preset temperatures with no power limit, and with a sensor that allows to know the temperature in that volume.

Description

TERMOCICLADOR ADAPTABLEADAPTABLE THERMOCICLATOR

5 Termociclador adaptable.5 Adaptive thermal cycler.

CAMPO DE LA INVENCIONFIELD OF THE INVENTION

La presente invencion se refiere a un termociclador adaptable sistema de 10 control de calidad, resistencia de materiales y procesos ciclicos. El termociclador ha sido concebido y realizado para obtener numerosas y notables ventajas respecto a otros medios existentes de analogas finalidades.The present invention relates to an adaptable thermal cycler system of quality control, resistance of materials and cyclic processes. The thermocycler has been conceived and realized to obtain numerous and notable advantages over other existing means of analogous purposes.

15 El termociclador esta previsto para lograr producir ciclos de calor y trio de una forma sencilla. Para ello, el termociclador cuenta con 12 partes bien diferenciadas que encajan entre si formando un unico objeto que es capaz de alternar las fases de calor y frio mediante energia electrica.15 The thermal cycler is designed to produce heat and trio cycles in a simple way. For this, the thermal cycler has 12 very different parts that fit together forming a single object that is capable of alternating the phases of heat and cold by electric energy.

20twenty

ESTADO DE LA TECNICA ANTERIORSTATE OF THE PREVIOUS TECHNIQUE

Se conocen varios sistemas y dispositivos capaces de someter a diversos materiales a ciclos alternantes de frio y calor.Several systems and devices are known capable of subjecting various materials to alternating cycles of cold and heat.

En tal sentido pueden citarse sistemas consistentes en un congelador en 25 cual se introducen los materiales y se programa el termostato a la temperatura deseada, una vez sometidas a dicha temperatura este se apaga dejando que vuelva subir la temperatura, si es necesario mayor temperatura que la ambiental, una vez alcanzada la temperatura ambiente se introduciran en una mufla hasta la temperatura deseada, tras lo cual 30 separa la mufla hasta que alcance la temperatura ambiente, momento en el que se repetira todo el proceso el numero de veces deseado.In this sense, systems consisting of a freezer can be mentioned in which the materials are introduced and the thermostat is programmed at the desired temperature, once subjected to this temperature it is turned off allowing the temperature to rise again, if a higher temperature is required than the temperature ambient, once the room temperature has been reached, they will be introduced in a muffle to the desired temperature, after which 30 the muffle separates until it reaches room temperature, at which time the entire process will be repeated the desired number of times.

Este sistema presenta diversos inconvenientes, tales como la necesidadThis system has several drawbacks, such as the need

de una mufla y un congelador tan grandes como los materiales a introducir, la necesidad de un operario dedicado a esta funcion que al ser poco intensiva en tiempo tienden a no ser rentable por el numero de horas y turnos de los empleados, o la prolongacion en el tiempo del proceso al 5 dejar el material de forma estable fuera de las horas de trabajo. Ademas, el proceso no es modular con lo que no se adapta a las diferentes necesidades del mercado segun la demanda. Tambien hay que tener en cuenta que a excepcion de las piezas de un tamano minimo, los volumenes, y pesos de estos sistemas, impiden su desplazamiento fuera 10 del laboratorio de pruebas de materiales.of a flask and a freezer as large as the materials to be introduced, the need for an operator dedicated to this function that being not very time intensive tends not to be profitable due to the number of hours and shifts of the employees, or the extension in the process time to 5 leave the material stably out of working hours. In addition, the process is not modular, so it does not adapt to different market needs according to demand. It should also be borne in mind that with the exception of the pieces of a minimum size, the volumes, and weights of these systems, prevent their displacement outside the laboratory of materials tests.

Igualmente, se conocen otros sistemas basados en microcontroladores programables cuyas salidas se encuentran unidas a modulos de Peltier, entradas a sensores de temperatura, luz, y tambien cuentan con una 15 interfase que permite al usuario programarlo en numero de ciclos y las condiciones de estos. Sin embargo la limitation en la potencia que pueden disipar estos controladores limita su amperaje, y con ello la potencia de los cambios termicos y de los materiales a termociclar.Likewise, other systems based on programmable microcontrollers are known whose outputs are linked to Peltier modules, inputs to temperature sensors, light, and also have an interface that allows the user to program it in number of cycles and their conditions. However, the limitation in the power that these controllers can dissipate limits their amperage, and with it the power of the thermal changes and of the materials to be thermocycled.

20twenty

EXPLICACION DE LA INVENCIONEXPLANATION OF THE INVENTION

El termociclador adaptable de la invencion presenta una nueva estrategia a la hora de generar los ciclos termicos: aprovechando la tecnologia Peltier permite invertir los flujos termicos con una inversion de voltaje 25 creando ciclos programados en un microcontrolador pero sin hacer pasar la corriente del Peltier por el microcontrolador, sino que el microcontrolador controlara los cambios de polaridad de la corriente de una fuente externa al microcontrolador y que alimenta el peltier, todo ello con una capa de aislante adaptable que permita diferentes formas y 30 volumenes, ademas de un sensor termico que determinen el punto del ciclo en el que se encuentra.The adaptable thermocycler of the invention presents a new strategy when generating thermal cycles: taking advantage of Peltier technology allows to reverse thermal fluxes with an inversion of voltage 25 creating programmed cycles in a microcontroller but without passing the Peltier current through the microcontroller, but the microcontroller will control the polarity changes of the current from an external source to the microcontroller and that feeds the peltier, all with an adaptive insulating layer that allows different shapes and 30 volumes, in addition to a thermal sensor that determines the point of the cycle in which it is located.

Ademas, se ha previsto que el Peltier cuente con un sistema de distribucion de energia, mediante un disipador con ventilador que favorezca los flujos termicos entre el material de prueba y el exterior del 5 habitaculo creado por la capa aislante.In addition, the Peltier is expected to have an energy distribution system, by means of a fan heatsink that favors thermal fluxes between the test material and the exterior of the room created by the insulating layer.

Tambien, se ha previsto que la capa aislante cuente con una superficie rugosa en su cara interna para favorecer los flujos termicos, asi como un perimetro de sellado.Also, it is envisioned that the insulating layer has a rough surface on its inner face to favor thermal fluxes, as well as a sealing perimeter.

1010

Igualmente existe la posibilidad de incluir varios peltier, bien sea para aumentar la potencia de los ciclos a que se somete a una muestra, como para someter a varias muestras a los mismos ciclos.There is also the possibility of including several peltier, either to increase the power of the cycles to which a sample is subjected, as to subject several samples to the same cycles.

15fifteen

BREVE DESCRIPCION DE LAS FIGURASBRIEF DESCRIPTION OF THE FIGURES

Para completar la descripcion que seguidamente se va a realizar, y con el objeto de ayudar a una mejor comprension de las caracteristicas del invento, se acompaha a la presente memoria descriptiva de un juego de 20 pianos, en base a cuyas figuras se comprenderan mas facilmente las innovaciones y ventajas del dispositivo objeto de la invencion.To complete the description that will be carried out below, and in order to help a better understanding of the features of the invention, the present specification of a set of 20 pianos is attached, based on whose figures will be more easily understood the innovations and advantages of the device object of the invention.

En dichos dibujos, la figura 1 representa el diagrama completo del termociclador adaptable, donde podemos distinguir el Microcontrolador (1) 25 que mediante el intercambiador de ciclos (3) altera la polaridad de la electricidad que reciben los peltier de las capas aislantes (2), proveniente de la fuente externa (4); la figura 2 representa el diagrama basico del termociclador adaptable donde podemos observar el Microcontrolador (1), el intercambiador de ciclos (3), la capa aislante (2), la fuente externa (4), 30 el aislante deformable (6), el sistema de distribucion de energia (7), y el sensor (5); la figura 3 representa el diagrama esquematico delIn said drawings, figure 1 represents the complete diagram of the adaptable thermocycler, where we can distinguish the Microcontroller (1) 25 which through the cycle exchanger (3) alters the polarity of the electricity received by the peltier of the insulating layers (2) , from the external source (4); Figure 2 represents the basic diagram of the adaptable thermocycler where we can see the Microcontroller (1), the cycle exchanger (3), the insulating layer (2), the external source (4), the deformable insulator (6), the power distribution system (7), and the sensor (5); Figure 3 represents the schematic diagram of the

termociclador adaptable donde podemos observar el peltier (8), el Microcontrolador (1), el intercambiador de ciclos (3), la fuente externa (4) y el sensor (5); la figura 4 representa en detalle, el intercambiador de ciclos, del diagrama esquematico del termociclador adaptable donde 5 podemos observar la parte fija (9), la parte movil (11), el servo (10) y el Microcontrolador (1); la figura 5 representa en detalle la capa aislante del diagrama esquematico del termociclador adaptable, donde podemos observar el aislante deformable (6) y el sistema de distribucion de energia (7); la figura 6 representa en detalle el sistema de distribucion de energia 10 de la capa aislante, del diagrama esquematico del termociclador adaptable donde podemos observar el Peltier (8), el disipador (12) y el ventilador (13); la figura 7 representa en detalle el aislante deformable de la capa aislante, del diagrama esquematico del termociclador adaptable donde podemos observar el aislante de vacio (14), las laminas 15 refractarias (15), la superficie rugosa (16) y el perlmetro de sellado (17).adaptable thermal cycler where we can observe the peltier (8), the Microcontroller (1), the cycle exchanger (3), the external source (4) and the sensor (5); Figure 4 represents in detail, the cycle exchanger, of the schematic diagram of the adaptable thermal cycler where we can observe the fixed part (9), the mobile part (11), the servo (10) and the Microcontroller (1); Figure 5 represents in detail the insulating layer of the schematic diagram of the adaptable thermocycler, where we can observe the deformable insulator (6) and the energy distribution system (7); Figure 6 represents in detail the energy distribution system 10 of the insulating layer, of the schematic diagram of the adaptable thermal cycler where we can observe the Peltier (8), the heatsink (12) and the fan (13); Figure 7 represents in detail the deformable insulator of the insulating layer, of the schematic diagram of the adaptable thermocycler where we can observe the vacuum insulator (14), the refractory sheets 15 (15), the rough surface (16) and the sealing perimeter (17).

EXPOSICION DETALLADA DE MODOS DE REALIZACIONDETAILED EXHIBITION OF MODES OF EMBODIMENT

En la actualidad existen muy diferentes materiales con los que realizar las 20 diversas partes de termociclador, y multiples tecnicas que podriamos utilizar en la confeccion del intercambiador de ciclos y de la capa aislante. No obstante, por simple economia elegiremos materiales y tecnicas generalizadas, que resistan los cambios termicos. Asi pues, para el disipador elegiremos aluminio mecanizado al igual que la parte fija y la 25 parte movil del intercambiador de ciclos. El aislante de vacio de la capa aislante sera de carton reforzado con goma EVA, la superficie rugosa y el perlmetro de sellado seran de goma EVA con polvo de Nd2Fe14B suspendido en su matriz. Las laminas refractarias seran de acero inoxidable con un grosor de 0.050mm, la fuente externa, el sensor, el 30 peltier, el servo y el ventilador seran de los disponibles comercialmente alAt present there are very different materials with which to make the 20 different parts of thermocycler, and multiple techniques that we could use in the preparation of the cycle exchanger and the insulating layer. However, for simple economy we will choose generalized materials and techniques that resist thermal changes. Thus, for the heat sink we will choose machined aluminum as well as the fixed part and the mobile part of the cycle exchanger. The vacuum insulation of the insulating layer will be made of cardboard reinforced with EVA rubber, the rough surface and the sealing perimeter will be made of EVA rubber with Nd2Fe14B powder suspended in its matrix. The refractory sheets will be stainless steel with a thickness of 0.050mm, the external source, the sensor, the 30 peltier, the servo and the fan will be commercially available from the

igual que el Microcontrolador, solo que este sera de los mas populares de forma que facilite su programacion.Like the Microcontroller, only this will be the most popular in a way that facilitates its programming.

En consecuencia, para testar la respuesta a cambios de temperatura de 5 un nuevo tipo de adoquin de hormigon, envolvemos el adoquin junto con el sensor en la capa aislante sellandola mediante el perlmetro de sellado, dejando despejado el sistema de distribution de energia y conectando este a la fuente externa a traves del intercambiador de ciclos. Repetiremos este proceso sin sensor hasta el numero de adoquines 10 deseados en la prueba, a continuation introduciremos en elConsequently, to test the response to changes in temperature of a new type of concrete paver, we wrap the paver together with the sensor in the insulating layer by sealing it by the sealing perimeter, leaving the energy distribution system clear and connecting this to the external source through the cycle exchanger. We will repeat this process without sensor up to the number of cobblestones 10 desired in the test, then we will introduce in the

microcontrolador la temperatura maxima y minima y el numero de ciclos, los cuales se conseguiran mediante el giro de la parte movil delmicrocontroller the maximum and minimum temperature and the number of cycles, which will be achieved by turning the mobile part of the

intercambiador de ciclos mediante el servo girando con respecto a la parte fija. La velocidad del cambio la definiremos en la fuente externa mediante 15 la intensidad y de voltaje de salida, que regularan la cantidad deCycle exchanger by means of the servo rotating with respect to the fixed part. The speed of the change will be defined in the external source by means of the intensity and output voltage, which will regulate the amount of

electricidad que pasara por el Peltier y la velocidad del ventilador queelectricity that will pass through the Peltier and the fan speed that

permitira bombear energia, en uno u otro sentido, a traves de loswill allow to pump energy, in one way or another, through the

disipadores y distribuyendola, gracias al flujo de aire que se creara a traves de la superficie rugosa del aislantes deformable.heatsinks and distributing it, thanks to the flow of air that will be created through the rough surface of the deformable insulators.

20twenty

Seran independientes del objeto de la invention los materiales empleados en la fabrication de los componentes del termociclador adaptable, formas y dimensiones de los mismos, y todos los detalles accesorios que puedan presentarse, siempre y cuando no afecten a su esencialidad.The materials used in the manufacture of the adaptable thermocycler components, shapes and dimensions thereof, and all the accessory details that may arise, will be independent of the object of the invention, provided they do not affect their essentiality.

Claims (1)

REIVINDICACIONES 1. Termociclador adaptable en sistemas de calidad, resistencia de materiales y procesos ciclicos, caracterizado por comprender doce 5 piezas; el Microcontrolador; las capas aislantes (el aislante de1. Adaptable thermocycler in quality systems, resistance of materials and cyclic processes, characterized by comprising twelve 5 pieces; the microcontroller; the insulating layers (the insulator of vaclo, las laminas refractarias y la superficie rugosa); el intercambiador de ciclos (la parte fija y la parte movil); la fuente externa; el sensor; el aislante deformable (las capas aislantes y el perlmetro de sellado); el sistema de distribucion de energla (el 10 Peltier, el disipador; y el ventilador); y el servo; acoplables entre sivacuum, refractory sheets and rough surface); the cycle exchanger (the fixed part and the mobile part); the external source; the sensor; the deformable insulator (the insulating layers and the sealing perimeter); the energy distribution system (the 10 Peltier, the heatsink; and the fan); and the servo; attachable with each other creando el termociclador adaptable, el cual mediante la informacion que proporciona el sensor al Microcontrolador que controla el servo del intercambiador de ciclos, hace girar la parte movil del intercambiador de ciclos sobre la parte fija del intercambiador de 15 ciclos, alterando la polaridad de la fuente externa de electricidadcreating the adaptable thermocycler, which by means of the information provided by the sensor to the Microcontroller that controls the servo of the cycle exchanger, rotates the mobile part of the cycle exchanger over the fixed part of the 15-cycle exchanger, altering the polarity of the source external electricity que alimenta el Peltier que bombea la energia calorifica a traves del disipador gracias al flujo de aire proporcionado por el ventilador, y que cambiaran la temperatura del contenido de las capas aislantes gracias a la superficie rugosa del aislante deformable que 20 ademas aisla mediante el aislante de vacio de las perdidasthat feeds the Peltier that pumps the energy heat through the heatsink thanks to the air flow provided by the fan, and that will change the temperature of the content of the insulating layers thanks to the rough surface of the deformable insulator that 20 also insulates by means of the insulator of empty of losses energeticas por conveccion, las laminas refractarias por radiacion y el perimetro de sellado del intercambio de aire a diferentes temperaturas. el Microcontrolador se encuentra unido al sensor y al servo, el servo se une tambien a la parte fija del intercambiador 25 de ciclos, la parte fija del intercambiador de ciclos esta unidaenergy by convection, the refractory sheets by radiation and the perimeter of sealing of the exchange of air at different temperatures. The Microcontroller is attached to the sensor and the servo, the servo is also attached to the fixed part of the cycle exchanger 25, the fixed part of the cycle exchanger is connected ademas a la parte movil del intercambiador de ciclos, la parte movil del intercambiador de ciclos esta unida tambien a la fuente externa y al Peltier, el Peltier forma parte del sistema de distribucion de energia teniendo a cada lado un disipador, y el disipador tambien 30 tiene unido un ventilador, siendo el ventilador la ultima pieza delin addition to the mobile part of the cycle exchanger, the mobile part of the cycle exchanger is also connected to the external source and the Peltier, the Peltier is part of the energy distribution system having on each side a heatsink, and the heatsink also 30 has a fan attached, the fan being the last piece of the sistema de distribucion de energia, unido al perfil del Peltier, seenergy distribution system, together with the Peltier profile, is encuentran las capas aislantes (el aislante de vacio, las laminas refractarias y la superficie rugosa); el aislante de vacio se encuentra en el interior de todas las capas, y esta rodeada por las laminas refractarias, una de las laminas refractarias se encuentra 5 unida ademas a la superficie rugosa y a perimetro de sellado, elthey find the insulating layers (the vacuum insulator, the refractory sheets and the rough surface); the vacuum insulator is inside all layers, and is surrounded by refractory sheets, one of the refractory sheets is also attached to the rough surface and perimeter of sealing, the perimetro de sellado rodea a la superficie rugosa, el perimetro de sellado mas las capas aislantes forman el aislante deformable.sealing perimeter surrounds the rough surface, the sealing perimeter plus the insulating layers form the deformable insulator.
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Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0342155A3 (en) * 1988-05-13 1990-06-27 Agrogen-Stiftung Laboratory device for optional heating and cooling
GB2261111A (en) * 1991-09-26 1993-05-05 Stuart Scient Company Limited Apparatus and method for heating and/or cooling reaction vessels
WO2007146443A2 (en) * 2006-06-14 2007-12-21 Oldenburg Kevin R Ph D Thermal-cycling devices and methods of using the same
US9492825B2 (en) * 2007-09-06 2016-11-15 It-Is International Limited Thermal control apparatus for chemical and biochemical reactions
GB201016014D0 (en) * 2010-09-24 2010-11-10 Epistem Ltd Thermal cycler

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