ES2570460T3 - Método de obtención de ácido nucleico que codifica inmunoglobulina - Google Patents

Método de obtención de ácido nucleico que codifica inmunoglobulina

Info

Publication number
ES2570460T3
ES2570460T3 ES10705301T ES10705301T ES2570460T3 ES 2570460 T3 ES2570460 T3 ES 2570460T3 ES 10705301 T ES10705301 T ES 10705301T ES 10705301 T ES10705301 T ES 10705301T ES 2570460 T3 ES2570460 T3 ES 2570460T3
Authority
ES
Spain
Prior art keywords
chain reaction
primer
nucleic acid
polymerase chain
primers
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES10705301T
Other languages
English (en)
Inventor
Hans Willi Krell
Alexander Lifke
Valeria Lifke
Kairat Madin
Christian Weilke
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
F Hoffmann La Roche AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Application granted granted Critical
Publication of ES2570460T3 publication Critical patent/ES2570460T3/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6881—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/21—Immunoglobulins specific features characterized by taxonomic origin from primates, e.g. man
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55—Fab or Fab'
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2549/00—Reactions characterised by the features used to influence the efficiency or specificity
    • C12Q2549/10—Reactions characterised by the features used to influence the efficiency or specificity the purpose being that of reducing false positive or false negative signals
    • C12Q2549/119—Reactions characterised by the features used to influence the efficiency or specificity the purpose being that of reducing false positive or false negative signals using nested primers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Método de producción de un fragmento Fab de inmunoglobulina que comprende las siguientes etapas: - proporcionar una célula única productora de inmunoglobulina, - obtener a partir de dicha célula el ácido nucleico que codifica los dominios variables de la cadena ligera y pesada de inmunoglobulina, que también codifica una parte del dominio constante de la cadena ligera y una parte del dominio CH1 de la cadena pesada, con un método que comprende las siguientes etapas: - realizar una primera reacción en cadena de la polimerasa con cuatro a seis cebadores 5' y un cebador 3', - realizar con el producto de la primera reacción en cadena de la polimerasa una segunda reacción en cadena de la polimerasa con trece a quince cebadores 5' y un cebador 3', por el cual en la segunda reacción en cadena de la polimerasa el cebador 3' es el mismo que en la primera reacción en cadena de la polimerasa y al menos un cebador 5' se cambia, y por el cual en la segunda reacción en cadena de la polimerasa el número de nucleótidos entre el extremo 5' de cada uno de los cebadores 5' y el extremo 3' del cebador 3' se reduce en comparación con el número de nucleótidos entre el extremo 5' de cada uno de los cebadores 5' y el extremo 3' del cebador 3' en la primera reacción en cadena de la polimerasa, cuando se une al ácido nucleico que va a amplificarse, - generar una matriz de expresión lineal que comprende el ácido nucleico obtenido, - traducir in vitro dicho ácido nucleico y así producir el fragmento Fab de inmunoglobulina.
ES10705301T 2009-02-20 2010-02-18 Método de obtención de ácido nucleico que codifica inmunoglobulina Active ES2570460T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP09002396 2009-02-20
PCT/EP2010/001008 WO2010094475A1 (en) 2009-02-20 2010-02-18 Method for obtaining immunoglobulin encoding nucleic acid

Publications (1)

Publication Number Publication Date
ES2570460T3 true ES2570460T3 (es) 2016-05-18

Family

ID=40790896

Family Applications (1)

Application Number Title Priority Date Filing Date
ES10705301T Active ES2570460T3 (es) 2009-02-20 2010-02-18 Método de obtención de ácido nucleico que codifica inmunoglobulina

Country Status (8)

Country Link
US (3) US9399670B2 (es)
EP (1) EP2398913B1 (es)
JP (2) JP5684734B2 (es)
CN (1) CN102325903B (es)
CA (1) CA2751669C (es)
ES (1) ES2570460T3 (es)
SG (1) SG173669A1 (es)
WO (1) WO2010094475A1 (es)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2570460T3 (es) 2009-02-20 2016-05-18 Hoffmann La Roche Método de obtención de ácido nucleico que codifica inmunoglobulina
CN103687873B (zh) * 2011-07-12 2016-10-19 埃克斯生物科技公司 鉴定亲和力成熟的人类抗体
BR112014004566A2 (pt) * 2011-09-21 2017-06-13 Hoffmann La Roche método de amplificação e quantificação de cadeias leve e pesada de igg cognato, método de obtenção de anticorpo monoclonal, métodos de produção de fragmento de fab de imunoglobulina e de imunoglobulina, ácido nucleico e kit
US10329545B2 (en) 2014-11-14 2019-06-25 Illumina, Inc. Polymerases
WO2019046817A1 (en) 2017-09-01 2019-03-07 Life Technologies Corporation COMPOSITIONS AND METHODS FOR IMMUNOLOGICAL REPERTOIRE SEQUENCING
WO2020018837A1 (en) * 2018-07-18 2020-01-23 Life Technologies Corporation Compositions and methods for immune repertoire sequencing
WO2020018836A1 (en) 2018-07-18 2020-01-23 Life Technologies Corporation Compositions and methods for immune repertoire sequencing
EP4093887A1 (en) * 2020-01-22 2022-11-30 Life Technologies Corporation Immune repertoire biomarkers in autoimmune disease and immunodeficiency disorders
JP2023184046A (ja) * 2022-06-17 2023-12-28 株式会社日立製作所 セルソータ用細胞懸濁液を改良した高感度な単一細胞解析方法

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3101690B2 (ja) 1987-03-18 2000-10-23 エス・ビィ・２・インコーポレイテッド 変性抗体の、または変性抗体に関する改良
US5204244A (en) 1987-10-27 1993-04-20 Oncogen Production of chimeric antibodies by homologous recombination
US5202238A (en) 1987-10-27 1993-04-13 Oncogen Production of chimeric antibodies by homologous recombination
US5478730A (en) 1988-12-21 1995-12-26 Institute Of Protein Research Method of preparing polypeptides in cell-free translation system
US5571690A (en) 1991-05-08 1996-11-05 The University Of Virginia Patents Foundation Method for the cell-free synthesis of proteins
GB9613418D0 (en) 1996-06-26 1996-08-28 Crc Technology Ltd A cell-free system for initiation of DNA replication
EP0953055A1 (en) 1997-01-21 1999-11-03 Daniel Favre Production of biologically active polypeptides
RU2148649C1 (ru) 1998-03-31 2000-05-10 Институт белка РАН Способ получения полипептидов в бесклеточной системе (варианты) и устройство для его осуществления
WO2000055353A1 (en) 1999-03-17 2000-09-21 The Board Of Trustees Of The Leland Stanford Junior University In vitro macromolecule biosynthesis methods using exogenous amino acids and a novel atp regeneration system
RU2169154C2 (ru) 1999-03-25 2001-06-20 Институт белка РАН Способ получения полипептидов в бесклеточной системе
KR20030038693A (ko) * 2000-08-11 2003-05-16 파브릴, 인크. Ｂ 세포 매개 병증을 개선시키기 위한 방법 및 조성물
US7425328B2 (en) 2003-04-22 2008-09-16 Purdue Pharma L.P. Tissue factor antibodies and uses thereof
CA2542656A1 (en) 2003-10-16 2005-05-06 Genomic Health, Inc. Qrt-pcr assay system for gene expression profiling
DE502005011243D1 (de) 2004-05-26 2011-05-26 Fraunhofer Ges Forschung Isolation allergen-spezifischer immunoglobulin-gene aus humanen b-zellen von atopikern
DK2121920T3 (da) 2007-03-01 2011-11-21 Symphogen As Fremgangsmåde til kloning af sammenhørende antistoffer
JP5205597B2 (ja) 2007-06-01 2013-06-05 静岡県 １細胞レベルでの抗体遺伝子の解析・同定方法
ES2570460T3 (es) 2009-02-20 2016-05-18 Hoffmann La Roche Método de obtención de ácido nucleico que codifica inmunoglobulina
US20130029340A1 (en) 2010-01-15 2013-01-31 Steffen Mergemeier Method for detecting more than one target in a pcr-based approach applying an un-specific dye which is not interfering with the emission of fluorophore-labeled probes

Also Published As

Publication number Publication date
CN102325903A (zh) 2012-01-18
US20110306094A1 (en) 2011-12-15
US20170107550A1 (en) 2017-04-20
JP2012518392A (ja) 2012-08-16
CA2751669A1 (en) 2010-08-26
EP2398913A1 (en) 2011-12-28
CN102325903B (zh) 2015-06-03
SG173669A1 (en) 2011-09-29
WO2010094475A1 (en) 2010-08-26
US9399670B2 (en) 2016-07-26
US20250137028A1 (en) 2025-05-01
EP2398913B1 (en) 2016-03-30
CA2751669C (en) 2017-11-21
JP5684734B2 (ja) 2015-03-18
JP2014221069A (ja) 2014-11-27

Similar Documents

Publication Publication Date Title
ES2570460T3 (es) Método de obtención de ácido nucleico que codifica inmunoglobulina
CY1125029T1 (el) Τροποποιημενοι νουκλεοζιτες, νουκλεοτιδια, και νουκλεϊκα οξεα και χρησεις αυτων
ECSP14013220A (es) Moléculas biespecíficas de unión a antígeno activadoras de células t
AR120511A2 (es) Producción de proteínas heteromultiméricas
AR106201A1 (es) Moléculas biespecíficas de unión a antígeno activadoras de células t
AR109771A1 (es) Moléculas biespecíficas de unión a antígeno activadoras de células t
ECSP14004893A (es) Moléculas de unión para bcma y cd3
ATE427965T1 (de) Antikírper gegen 25-hydroxyvitamin d
MX2009007745A (es) Sistema y metodo para generar instrucciones para personalizar.
CL2009000898A1 (es) Cepa de levadura transformada que tiene alterado el transportador de urea; metodo para modificar dicha levadura; util para producir una bebida o producto alimenticio fermentado, asi como el producto producido con dicha levadura.
BR112013018377A2 (pt) processo para produção de microalgas, cianobactérias e seus metabólitos
EP4410986A3 (en) Cells, vertebrates, populations & methods
EA201890028A1 (ru) Антитело, связывающее cd3 человека
AR094896A1 (es) Moléculas biespecíficas de unión a antígeno activadoras de células t
BR112013016321A8 (pt) Composição vulcanizável, processo para a produção de uma composição vulcanizável, processo para a produção de vulcanizados com base em uma borracha de nitrila hidrogenada, vulcanizado obtenível pelo processo e borracha de nitrila hidrogenada
CY1119173T1 (el) Νεα μορια αναστολεα jnk
BR112015020904A2 (pt) microrganismos e métodos para produção de butadieno e compostos relacionados por assimilação de formiato
BRPI0916147A8 (pt) Cepa de saccharomyces ap., método para a produção de um produto de fermentação e massa celular, e, uso da cepa de saccharomyces sp.
BR112017009223A2 (pt) módulo de biorreator eletroquímico melhorado e uso do mesmo
BR112015022529A2 (pt) meios de cultura de células e processos de produção de anticorpo
PH12020551948A1 (en) Monospecific and multispecific anti-tmeff2 antibodies and their uses
FI20115832A7 (fi) Menetelmä erinomaisilla mekaanisilla ominaisuuksilla varustetun terästuotteen valmistamiseksi, menetelmällä valmistettu terästuote ja muokkauslujitetun teräksen käyttö
CL2017001814A1 (es) Un proceso para la síntesis de amoníaco.
MX2014005242A (es) Proceso para producir 2,3,3,3-tretrafluoropropeno.
BR112015015786A2 (pt) composição de polietileno reticulado, processo para preparar uma composição de polietileno reticulado, artigo manufaturado, tubo e método para produzir um tubo