ES2572630T3 - Procedimiento para el enriquecimiento de ácidos nucleicos de cadena corta - Google Patents

Procedimiento para el enriquecimiento de ácidos nucleicos de cadena corta Download PDF

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Publication number
ES2572630T3
ES2572630T3 ES06830479.9T ES06830479T ES2572630T3 ES 2572630 T3 ES2572630 T3 ES 2572630T3 ES 06830479 T ES06830479 T ES 06830479T ES 2572630 T3 ES2572630 T3 ES 2572630T3
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Prior art keywords
anion exchange
exchange matrix
nucleic acid
aqueous phase
nucleic acids
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Active
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ES06830479.9T
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English (en)
Inventor
Christoph Ritt
Ralf Himmelreich
Martin Weber
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Qiagen GmbH
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Qiagen GmbH
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
    • Y10T436/255—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.] including use of a solid sorbent, semipermeable membrane, or liquid extraction

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

Un procedimiento para enriquecer acidos nucleicos con una longitud de menos de 300 nucleotidos de una muestra que se selecciona del grupo constituido por plasma, suero, un fluido corporal, un frotis y un lisado celular, que comprende las etapas de procedimiento i) Proporcionar una fase fluida P1, preferiblemente acuosa, que contiene (α1) al menos un acido nucleico con una longitud de menos de 300 nucleotidos, asi como (α2) al menos un componente distinto de este acido nucleico (α1), ii) poner en contacto la fase P1 con una matriz de intercambio anionico para unir el acido nucleico (α1) a la matriz de intercambio anionico, realizandose la union de los acidos nucleicos a la matriz de intercambio anionico a un valor de pH en un intervalo de 3 a 7, presentando la matriz de intercambio anionico grupos funcionales seleccionados del grupo constituido por grupos amino, grupos hidracina y grupos imina que estan al menos parcialmente presentes en forma cationica en las condiciones bajo las que se pone en contacto la fase acuosa P1 con la matriz de intercambio anionico, y estando la matriz de intercambio anionico presente en forma de un recubrimiento sobre particulas magneticas y para la union de los acidos nucleicos la fase acuosa P1 puesta en contacto con las particulas se mueve continuamente, y separandose las particulas magneticas de la fase acuosa P1 como agregados magneticos, iii) dado el caso lavar la matriz de intercambio anionico con un tampon de lavado, permaneciendo el acido nucleico (α1) unido a la matriz de intercambio anionico, asi como iv) eluir el acido nucleico (α1) unido a la matriz de intercambio anionico de la matriz de intercambio anionico mediante la sustitucion de la fase acuosa P1 o del tampon de lavado con un tampon de elucion manteniendo una fase acuosa P2 que contiene el acido nucleico (α1).

Description

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Claims (1)

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ES06830479.9T 2005-12-09 2006-12-08 Procedimiento para el enriquecimiento de ácidos nucleicos de cadena corta Active ES2572630T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE102005059315A DE102005059315A1 (de) 2005-12-09 2005-12-09 Verfahren zur Anreicherung von kurzkettigen Nukleinsäuren
DE102005059315 2005-12-09
PCT/EP2006/069484 WO2007065950A1 (de) 2005-12-09 2006-12-08 Verfahren zur anreicherung von kurzkettigen nukleinsäuren

Publications (1)

Publication Number Publication Date
ES2572630T3 true ES2572630T3 (es) 2016-06-01

Family

ID=37813635

Family Applications (2)

Application Number Title Priority Date Filing Date
ES16157978T Active ES2729164T3 (es) 2005-12-09 2006-12-08 Procedimiento para el enriquecimiento de ácidos nucleicos de cadena corta
ES06830479.9T Active ES2572630T3 (es) 2005-12-09 2006-12-08 Procedimiento para el enriquecimiento de ácidos nucleicos de cadena corta

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ES16157978T Active ES2729164T3 (es) 2005-12-09 2006-12-08 Procedimiento para el enriquecimiento de ácidos nucleicos de cadena corta

Country Status (8)

Country Link
US (1) US7977109B2 (es)
EP (3) EP3064583B1 (es)
JP (1) JP5203960B2 (es)
CN (3) CN103173436A (es)
AU (1) AU2006323954B2 (es)
DE (1) DE102005059315A1 (es)
ES (2) ES2729164T3 (es)
WO (1) WO2007065950A1 (es)

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HUE019019T5 (en) * 2007-08-22 2017-04-28 Trovagene Inc Methods for Using miRNA for In vivo Cell Death Detection
KR101432034B1 (ko) 2007-11-16 2014-08-21 삼성전자주식회사 코스모트로픽 염을 이용한 고체 지지체에서의 작은 ｒｎａ분리 방법
JP6096660B2 (ja) * 2010-09-02 2017-03-15 キアゲン ゲーエムベーハー 低分子標的核酸を含む標的核酸を高収量で単離するための方法
WO2013002261A1 (ja) 2011-06-27 2013-01-03 オリンパス株式会社 標的粒子の検出方法
SG11201606921PA (en) 2014-01-28 2016-10-28 Dice Molecules Sv Llc Monoliths with attached recognition compounds, arrays thereof and uses thereof
SG11201608575QA (en) * 2014-04-18 2016-11-29 Toppan Printing Co Ltd Method for recovering short-chain nucleic acids
CN104059204B (zh) * 2014-04-28 2017-04-05 苏州工业园区为真生物医药科技有限公司 表面阳离子磁性聚合物微球及其制备方法和应用
WO2015183667A1 (en) * 2014-05-28 2015-12-03 The Regents Of The University Of California HYBRID tRNA/pre-miRNA MOLECULES AND METHODS OF USE
JP2017539080A (ja) 2014-10-23 2017-12-28 コーニング インコーポレイテッド 高分子被包磁性ナノ粒子
CA2965500A1 (en) 2014-10-24 2016-04-28 Abbott Molecular Inc. Enrichment of small nucleic acids
AU2017233436B2 (en) * 2016-03-18 2022-11-24 Hummingbird Diagnostics Gmbh Purification of RNA fractions using a hydrophilic polymeric material
WO2017162518A1 (en) 2016-03-19 2017-09-28 Qiagen Gmbh Stabilization of rna
KR102260012B1 (ko) * 2020-09-29 2021-06-03 주식회사 에이아이더뉴트리진 미생물 농축 또는 핵산 추출용 조성물 및 이를 이용한 미생물 농축 또는 핵산 추출 방법
US20240287498A1 (en) * 2021-06-17 2024-08-29 Qiagen Gmbh Method for isolating non-vesicular mirna
CN117050120A (zh) * 2022-12-09 2023-11-14 苏州赛分科技股份有限公司 一种纯化小干扰rna的柱层析法

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Also Published As

Publication number Publication date
WO2007065950A1 (de) 2007-06-14
EP3064583A1 (de) 2016-09-07
EP3064583B1 (de) 2019-03-27
EP1960522B1 (de) 2016-03-02
JP2009518020A (ja) 2009-05-07
AU2006323954A1 (en) 2007-06-14
CN103173436A (zh) 2013-06-26
US20090081802A1 (en) 2009-03-26
DE102005059315A1 (de) 2007-06-14
ES2729164T3 (es) 2019-10-30
AU2006323954B2 (en) 2012-05-10
EP1960522A1 (de) 2008-08-27
EP3524678A1 (de) 2019-08-14
CN105018470B (zh) 2025-08-29
US7977109B2 (en) 2011-07-12
JP5203960B2 (ja) 2013-06-05
CN105018470A (zh) 2015-11-04
CN101326284A (zh) 2008-12-17

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