ES2603128T3 - Microorganismo con productividad potenciada de L-lisina y procedimiento de producción de L-lisina usando el mismo - Google Patents

Microorganismo con productividad potenciada de L-lisina y procedimiento de producción de L-lisina usando el mismo Download PDF

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Publication number
ES2603128T3
ES2603128T3 ES11791415.0T ES11791415T ES2603128T3 ES 2603128 T3 ES2603128 T3 ES 2603128T3 ES 11791415 T ES11791415 T ES 11791415T ES 2603128 T3 ES2603128 T3 ES 2603128T3
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Spain
Prior art keywords
lysine
microorganism
gntk
same
enhanced
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Active
Application number
ES11791415.0T
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English (en)
Inventor
Sang Jo Lim
Jun Ok Moon
Hyung Joon Kim
Jae Woo Jang
Jae Yong Cho
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CJ CheilJedang Corp
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CJ CheilJedang Corp
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Priority claimed from PCT/KR2011/005252 external-priority patent/WO2012008810A2/en
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Publication of ES2603128T3 publication Critical patent/ES2603128T3/es
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Un procedimiento de producción de L-lisina, que comprende las etapas de: 1) cultivar un microorganismo productor de L-lisina que pertenece a Corynebacterium spp., que tiene la actividad de una gluconato quinasa (GntK) debilitada en comparación con la actividad endógena de la misma en Corynebacterium spp. de tipo silvestre, en el que la GntK tiene la secuencia de aminoácidos de la SEQ ID NO: 2, para obtener un cultivo celular; y 2) recoger L-lisina del cultivo celular o el microorganismo, en el que la actividad GntK está debilitada por deleción completa o parcial, sustitución o inserción parcial: - en un gen cromosómico que codifica gluconato quinasa o - en un elemento regulador para un gen cromosómico que codifica gluconato quinasa.

Description

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Claims (1)

  1. imagen1
ES11791415.0T 2010-07-15 2011-07-15 Microorganismo con productividad potenciada de L-lisina y procedimiento de producción de L-lisina usando el mismo Active ES2603128T3 (es)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
KR20100068618 2010-07-15
KR20100068618 2010-07-15
KR20110065694 2011-07-01
KR1020110065694A KR101269810B1 (ko) 2010-07-15 2011-07-01 Ｌ－라이신 생산능이 향상된 미생물 및 이를 이용한 ｌ－라이신 생산방법
PCT/KR2011/005252 WO2012008810A2 (en) 2010-07-15 2011-07-15 Microorganism with enhanced l-lysine productivity and method for producing l-lysine using the same

Publications (1)

Publication Number Publication Date
ES2603128T3 true ES2603128T3 (es) 2017-02-23

Family

ID=45613138

Family Applications (1)

Application Number Title Priority Date Filing Date
ES11791415.0T Active ES2603128T3 (es) 2010-07-15 2011-07-15 Microorganismo con productividad potenciada de L-lisina y procedimiento de producción de L-lisina usando el mismo

Country Status (8)

Country Link
US (1) US20130102037A1 (es)
JP (1) JP5682072B2 (es)
KR (1) KR101269810B1 (es)
CN (1) CN102741393B (es)
BR (1) BRPI1106092A2 (es)
DK (1) DK2430152T3 (es)
ES (1) ES2603128T3 (es)
PL (1) PL2430152T3 (es)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101582008B1 (ko) 2013-10-15 2015-12-31 씨제이제일제당 (주) 생물막 형성 억제 활성을 가지는 유전자 및 이 유전자가 불활성화된 균주를 이용한 l-라이신 생산 방법
KR101640711B1 (ko) * 2015-04-20 2016-07-19 씨제이제일제당 (주) 글루코네이트 리프레서 변이체, 이를 포함하는 l-라이신을 생산하는 미생물 및 이를 이용한 ｌ－라이신 생산방법
KR101793328B1 (ko) * 2015-07-03 2017-11-03 씨제이제일제당 (주) Ｌ-라이신 생산능을 갖는 미생물 및 이를 이용한 ｌ-라이신 생산 방법
KR101766964B1 (ko) * 2015-08-27 2017-08-09 씨제이제일제당 (주) L-라이신 생산능을 가지는 코리네박테리움 속 미생물 및 이를 이용한 l-라이신 생산방법
KR102011394B1 (ko) * 2017-07-19 2019-10-22 씨제이제일제당 주식회사 퓨트레신을 생산하는 미생물 및 이를 이용한 퓨트레신 생산방법
WO2025244454A1 (ko) * 2024-05-24 2025-11-27 씨제이제일제당 (주) 신규한 단백질 또는 폴리뉴클레오티드 변이체 및 이를 이용한 l-라이신 생산 방법

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4623825B2 (ja) 1999-12-16 2011-02-02 協和発酵バイオ株式会社 新規ポリヌクレオチド
KR100397322B1 (ko) * 2000-12-30 2003-09-06 씨제이 주식회사 엘-라이신의 제조방법
CN1298855C (zh) * 2001-04-04 2007-02-07 金克克国际有限公司 在宿主细胞中生成产物的方法
US7419795B2 (en) * 2003-05-22 2008-09-02 Danisco A/S, Genencor Division Metabolically engineered bacterial strains having non-functional endogenous gluconate transporters
KR100789271B1 (ko) * 2005-11-30 2008-01-02 씨제이 주식회사 Ｌ-라이신 생산능이 향상된 코리네박테리움 속 미생물 및그를 이용하여 ｌ-라이신을 생산하는 방법
KR100838035B1 (ko) 2006-12-29 2008-06-12 씨제이제일제당 (주) Ｌ-라이신 생산능이 향상된 코리네박테리움 속 미생물 및그를 이용한 ｌ-라이신 생산 방법
KR100838038B1 (ko) 2006-12-29 2008-06-12 씨제이제일제당 (주) Ｌ-라이신 생산능이 향상된 코리네박테리움 속 미생물 및그를 이용한 ｌ-라이신 생산 방법

Also Published As

Publication number Publication date
CN102741393A (zh) 2012-10-17
US20130102037A1 (en) 2013-04-25
DK2430152T3 (en) 2016-12-05
JP2012530515A (ja) 2012-12-06
CN102741393B (zh) 2015-11-25
BRPI1106092A2 (pt) 2016-10-11
KR101269810B1 (ko) 2013-05-30
JP5682072B2 (ja) 2015-03-11
PL2430152T3 (pl) 2017-02-28
KR20120007965A (ko) 2012-01-25

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