ES2613539T3 - Producción fermentativa de isobutanol con levaduras - Google Patents
Producción fermentativa de isobutanol con levaduras Download PDFInfo
- Publication number
- ES2613539T3 ES2613539T3 ES09713531.3T ES09713531T ES2613539T3 ES 2613539 T3 ES2613539 T3 ES 2613539T3 ES 09713531 T ES09713531 T ES 09713531T ES 2613539 T3 ES2613539 T3 ES 2613539T3
- Authority
- ES
- Spain
- Prior art keywords
- seq
- reaction
- genes
- catalyzes
- acetolactate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- ZXEKIIBDNHEJCQ-UHFFFAOYSA-N isobutanol Chemical compound CC(C)CO ZXEKIIBDNHEJCQ-UHFFFAOYSA-N 0.000 title abstract 8
- 240000004808 Saccharomyces cerevisiae Species 0.000 title 1
- 230000004151 fermentation Effects 0.000 title 1
- 238000000855 fermentation Methods 0.000 title 1
- 238000004519 manufacturing process Methods 0.000 title 1
- AMIMRNSIRUDHCM-UHFFFAOYSA-N Isopropylaldehyde Chemical compound CC(C)C=O AMIMRNSIRUDHCM-UHFFFAOYSA-N 0.000 abstract 6
- 108090000623 proteins and genes Proteins 0.000 abstract 4
- JTEYKUFKXGDTEU-UHFFFAOYSA-N 2,3-dihydroxy-3-methylbutanoic acid Chemical compound CC(C)(O)C(O)C(O)=O JTEYKUFKXGDTEU-UHFFFAOYSA-N 0.000 abstract 3
- WTLNOANVTIKPEE-UHFFFAOYSA-N 2-acetyloxypropanoic acid Chemical compound OC(=O)C(C)OC(C)=O WTLNOANVTIKPEE-UHFFFAOYSA-N 0.000 abstract 3
- QHKABHOOEWYVLI-UHFFFAOYSA-N 3-methyl-2-oxobutanoic acid Chemical compound CC(C)C(=O)C(O)=O QHKABHOOEWYVLI-UHFFFAOYSA-N 0.000 abstract 3
- 108010004277 2-acetolactate mutase Proteins 0.000 abstract 2
- 108010000700 Acetolactate synthase Proteins 0.000 abstract 2
- 108090000790 Enzymes Proteins 0.000 abstract 2
- 102000004190 Enzymes Human genes 0.000 abstract 2
- LCTONWCANYUPML-UHFFFAOYSA-M Pyruvate Chemical compound CC(=O)C([O-])=O LCTONWCANYUPML-UHFFFAOYSA-M 0.000 abstract 2
- 230000002438 mitochondrial effect Effects 0.000 abstract 2
- 210000005253 yeast cell Anatomy 0.000 abstract 2
- 102000007698 Alcohol dehydrogenase Human genes 0.000 abstract 1
- 108010021809 Alcohol dehydrogenase Proteins 0.000 abstract 1
- 108700028369 Alleles Proteins 0.000 abstract 1
- 108020004705 Codon Proteins 0.000 abstract 1
- 108700016168 Dihydroxy-acid dehydratases Proteins 0.000 abstract 1
- 101150111679 ILV5 gene Proteins 0.000 abstract 1
- 101100396751 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) ilv-2 gene Proteins 0.000 abstract 1
- 101100396749 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) ILV3 gene Proteins 0.000 abstract 1
- 101100082596 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) PDC5 gene Proteins 0.000 abstract 1
- 101100519200 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) PDC6 gene Proteins 0.000 abstract 1
- 239000002253 acid Substances 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
- 101150045896 ilv-2 gene Proteins 0.000 abstract 1
- 230000002503 metabolic effect Effects 0.000 abstract 1
- 150000007523 nucleic acids Chemical group 0.000 abstract 1
- 101150017059 pcd1 gene Proteins 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/16—Butanols
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1022—Transferases (2.) transferring aldehyde or ketonic groups (2.2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
Célula de levadura que produce isobutanol, caracterizada por que la célula presenta un flujo de sustancias metabólico incrementado de piruvato a través de acetolactato, 2,3-dihidroxiisovalerato, 2-cetoisovalerato, isobutiraldehído para formar isobutanol, en donde todos los genes que codifican las enzimas que participan en esta reacción están sobre-expresados, y por que estos genes son homólogos a dicha célula de levadura, en donde Ilv2 con SEQ ID Nº 2 cataliza la reacción de acetolactato sintasa de piruvato para formar acetolactato, ilv5 con SEQ ID Nº 6 cataliza la reacción de acetohidroxiácido reductoisomerasa de acetolactato para formar 2,3-dihidroxiisovalerato, Ilv3 con SEQ ID Nº 8 cataliza la reacción de dihidroxiácido deshidratasa de 2,3-dihidroxiisovalerato para formar 2- cetoisovalerato, una 2-cetoácido descarboxilasa cataliza la reacción de 2-cetoisovalerato para formar isobutiraldehído y una alcohol deshidrogenasa cataliza la reacción de isobutiraldehído para formar isobutanol, en donde al menos uno de los promotores de estos genes es intercambiado por al menos un promotor más fuerte, o las secuencias de ácidos nucleicos de estos genes son transformadas en alelos optimizados en codones, caracterizada por que se sobre-expresan los genes ILV2, ILV5 e ILV3 sin la secuencia diana mitocondrial de las enzimas acetolactato sintasa, acetohidroxiácido reductoisomerasa y dihidroxiácido deshidratasa o se sobre expresan con una secuencia diana mitocondrial destruida e inactivada, y caracterizada porque, adicionalmente, se reduce o bien se suprime la expresión de los genes PCD1 con SEQ. ID. No. 13, PDC5 con SEQ. ID. No. 15 y PDC6 con SEQ. ID. No. 17 o bien la actividad de las enzimas codificadas.
Description
Claims (1)
-
imagen1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE102008010121A DE102008010121B4 (de) | 2008-02-20 | 2008-02-20 | Fermentative Produktion von Isobutanol mit Hefe |
| DE102008010121 | 2008-02-20 | ||
| PCT/EP2009/001191 WO2009103533A1 (de) | 2008-02-20 | 2009-02-19 | Fermentative produktion von isobutanol mit hefe |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2613539T3 true ES2613539T3 (es) | 2017-05-24 |
Family
ID=40718833
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES09713531.3T Active ES2613539T3 (es) | 2008-02-20 | 2009-02-19 | Producción fermentativa de isobutanol con levaduras |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US8530226B2 (es) |
| EP (1) | EP2255007B1 (es) |
| BR (1) | BRPI0913670B1 (es) |
| CA (1) | CA2716427C (es) |
| DE (1) | DE102008010121B4 (es) |
| DK (1) | DK2255007T3 (es) |
| ES (1) | ES2613539T3 (es) |
| HR (1) | HRP20170096T1 (es) |
| HU (1) | HUE031212T2 (es) |
| PL (1) | PL2255007T3 (es) |
| PT (1) | PT2255007T (es) |
| WO (1) | WO2009103533A1 (es) |
Families Citing this family (31)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9303225B2 (en) | 2005-10-26 | 2016-04-05 | Butamax Advanced Biofuels Llc | Method for the production of isobutanol by recombinant yeast |
| US8273558B2 (en) | 2005-10-26 | 2012-09-25 | Butamax(Tm) Advanced Biofuels Llc | Fermentive production of four carbon alcohols |
| RU2394913C2 (ru) | 2005-10-26 | 2010-07-20 | Е.И.Дюпон Де Немур Энд Компани | Ферментативное получение четырехуглеродных спиртов |
| US8455239B2 (en) | 2007-12-23 | 2013-06-04 | Gevo, Inc. | Yeast organism producing isobutanol at a high yield |
| CA2710359C (en) | 2007-12-23 | 2018-02-20 | Gevo, Inc. | Yeast organism producing isobutanol at a high yield |
| MY153731A (en) | 2007-12-27 | 2015-03-13 | Gevo Inc | Recovery of higher alcohols from dilute aqueous solutions |
| BRPI0909989A2 (pt) | 2008-06-05 | 2021-06-22 | Butamax Advanced Biofuels Llc | célula de levedura recombinante e método para a produção de um produto |
| ES2521675T3 (es) | 2008-09-29 | 2014-11-13 | Butamax Advanced Biofuels Llc | Identificación y uso de [2Fe-2S]-dihidroxi-ácido deshidratasas bacterianas |
| NZ591126A (en) | 2008-09-29 | 2012-08-31 | Butamax Tm Advanced Biofuels | INCREASED HETEROLOGOUS Fe-S ENZYME ACTIIVTY IN YEAST |
| US20160222370A1 (en) * | 2008-09-29 | 2016-08-04 | Butamax Advanced Biofuels Llc | Recombinant Yeast Host Cell With Fe-S Cluster Proteins And Methods Of Using Thereof |
| EP2464736A4 (en) * | 2009-08-12 | 2013-07-31 | Gevo Inc | PATIENT LOCALIZATION OF CYTOSOLIC ISOBUTANOL FOR THE MANUFACTURE OF ISOBUTANOL |
| EP2504422B1 (en) | 2009-11-24 | 2016-01-27 | GEVO, Inc. | Methods of increasing dihydroxy acid dehydratase activity to improve production of fuels, chemicals, and amino acids |
| US20120034666A1 (en) * | 2009-11-24 | 2012-02-09 | Gevo, Inc. | Methods of increasing dihydroxy acid dehydratase activity to improve production of fuels, chemicals, and amino acids |
| CA2783432A1 (en) | 2009-12-15 | 2011-06-23 | Stratley Ag | Method for recovery of organic components from dilute aqueous solutions |
| BR112012020261B8 (pt) * | 2010-02-12 | 2020-04-14 | California Inst Of Techn | saccharomyces cerevisiae recombinante e método de produção de isobutanol |
| BR112012020589B8 (pt) * | 2010-02-17 | 2021-05-18 | Butamax Tm Advanced Biofuels | célula hospedeira recombinante de levedura, método para fabricar um produto, método para converter 2,3-diidroxiisovalerato em alfa-cetoisovalerato, método para aumentar a atividade específica de um polipeptídeo heterólogo, método para aumentar o fluxo em uma via biossintética e método para aumentar a atividade de uma proteína |
| US9249420B2 (en) * | 2010-05-31 | 2016-02-02 | Vib Vzw | Isobutanol production using yeasts with modified transporter expression |
| US9012190B2 (en) | 2011-06-15 | 2015-04-21 | Butamax Advanced Biofuels Llc | Use of thiamine and nicotine adenine dinucleotide for butanol production |
| CA2810244C (en) * | 2010-09-07 | 2020-10-06 | Butamax(Tm) Advanced Biofuels Llc | Integration of a polynucleotide encoding a polypeptide that catalyzes pyruvate to acetolactate conversion |
| EP2508597A1 (en) | 2011-04-05 | 2012-10-10 | Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK) | Production of butanol by fermentation in Arxula sp. |
| WO2012173659A2 (en) * | 2011-06-17 | 2012-12-20 | Butamax (Tm) Advanced Biofuels Llc | Lignocellulosic hydrolysates as feedstocks for isobutanol fermentation |
| WO2013055667A2 (en) * | 2011-10-10 | 2013-04-18 | Regents Of The University Of Minnesota | Biocatalysis cells and methods |
| EP2583957A1 (de) | 2011-10-18 | 2013-04-24 | LANXESS Deutschland GmbH | Lineare Butene aus Isobutanol |
| JP2015503351A (ja) | 2011-12-30 | 2015-02-02 | ビュータマックス・アドバンスド・バイオフューエルズ・エルエルシー | ブタノール生産のための遺伝的スイッチ |
| US9650624B2 (en) | 2012-12-28 | 2017-05-16 | Butamax Advanced Biofuels Llc | DHAD variants for butanol production |
| WO2014151190A1 (en) | 2013-03-15 | 2014-09-25 | Butamax Advanced Biofuels Llc | Dhad variants and methods of screening |
| US9771602B2 (en) * | 2013-03-15 | 2017-09-26 | Butamax Advanced Biofuels Llc | Competitive growth and/or production advantage for butanologen microorganism |
| US9663759B2 (en) | 2013-07-03 | 2017-05-30 | Butamax Advanced Biofuels Llc | Partial adaptation for butanol production |
| US10829789B2 (en) | 2016-12-21 | 2020-11-10 | Creatus Biosciences Inc. | Methods and organism with increased xylose uptake |
| EP4114917A4 (en) | 2020-03-06 | 2024-08-21 | GEVO, Inc. | Novel nkr variants for increased production of isobutanol |
| US11788053B2 (en) | 2020-06-15 | 2023-10-17 | Melio Peptide Systems Inc. | Microorganisms and methods for reducing bacterial contamination |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2394913C2 (ru) * | 2005-10-26 | 2010-07-20 | Е.И.Дюпон Де Немур Энд Компани | Ферментативное получение четырехуглеродных спиртов |
-
2008
- 2008-02-20 DE DE102008010121A patent/DE102008010121B4/de not_active Expired - Fee Related
-
2009
- 2009-02-19 ES ES09713531.3T patent/ES2613539T3/es active Active
- 2009-02-19 EP EP09713531.3A patent/EP2255007B1/de active Active
- 2009-02-19 US US12/918,722 patent/US8530226B2/en not_active Expired - Fee Related
- 2009-02-19 HU HUE09713531A patent/HUE031212T2/en unknown
- 2009-02-19 PT PT97135313T patent/PT2255007T/pt unknown
- 2009-02-19 BR BRPI0913670-3A patent/BRPI0913670B1/pt not_active IP Right Cessation
- 2009-02-19 DK DK09713531.3T patent/DK2255007T3/en active
- 2009-02-19 CA CA2716427A patent/CA2716427C/en active Active
- 2009-02-19 PL PL09713531T patent/PL2255007T3/pl unknown
- 2009-02-19 WO PCT/EP2009/001191 patent/WO2009103533A1/de not_active Ceased
- 2009-02-19 HR HRP20170096TT patent/HRP20170096T1/hr unknown
Also Published As
| Publication number | Publication date |
|---|---|
| BRPI0913670A2 (pt) | 2016-07-26 |
| US8530226B2 (en) | 2013-09-10 |
| PT2255007T (pt) | 2017-02-08 |
| EP2255007B1 (de) | 2016-11-02 |
| PL2255007T3 (pl) | 2017-06-30 |
| BRPI0913670B1 (pt) | 2020-10-20 |
| WO2009103533A1 (de) | 2009-08-27 |
| HRP20170096T1 (hr) | 2017-03-24 |
| US20110053235A1 (en) | 2011-03-03 |
| CA2716427A1 (en) | 2009-08-27 |
| DE102008010121A1 (de) | 2009-09-03 |
| EP2255007A1 (de) | 2010-12-01 |
| CA2716427C (en) | 2017-08-29 |
| HUE031212T2 (en) | 2017-07-28 |
| DK2255007T3 (en) | 2017-02-06 |
| DE102008010121B4 (de) | 2013-11-21 |
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