ES2618730T3 - Nueva cepa fúngica para la producción de celulasa y método de sacarificación que la utiliza - Google Patents

Nueva cepa fúngica para la producción de celulasa y método de sacarificación que la utiliza Download PDF

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Publication number
ES2618730T3
ES2618730T3 ES13752441.9T ES13752441T ES2618730T3 ES 2618730 T3 ES2618730 T3 ES 2618730T3 ES 13752441 T ES13752441 T ES 13752441T ES 2618730 T3 ES2618730 T3 ES 2618730T3
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saccharification
fungal strain
cellulase
cellulase production
saccharification method
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ES13752441.9T
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English (en)
Inventor
Jung Kul Lee
Tae Su Kim
Sujit Sadashiv JAGTAP
Min Ho Cha
Jong In Lee
Hang Duk Roh
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SK Chemicals Co Ltd
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SK Chemicals Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/145—Fungi isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Molecular Biology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Una cepa SKU714 de Pholiota adiposa productora de celulasa depositada con el n.º de acceso KCCM 11187P.

Description

imagen1
imagen2
imagen3
imagen4
imagen5
(3) Rendimiento de sacarificación en función de la temperatura
Se investigó el efecto de la temperatura sobre la sacarificación de álamo mediante la enzima de sacarificación producida por la cepa SKU714 de Pholiota adiposa. Se midió el rendimiento de sacarificación a diferentes temperaturas de reacción de 20, 35, 50, 65 y 80°C. Se muestra el resultado en la Tabla 5.
Tabla 5
Temperatura de reacción (°C)
Rendimiento de sacarificación (%)
20
33,2
35
45,0
50
75,8
65
82,1
80
71,2
Tal como se observa a partir de la Tabla 5, se obtuvo un rendimiento de sacarificación superior cuando la temperatura era de 50-80°C. El mejor rendimiento de sacarificación se obtuvo a 65°C.
(4) Rendimiento de sacarificación en función del pH
10 Se investigó el efecto del pH sobre la sacarificación de álamo mediante la enzima de sacarificación producida por la cepa SKU714 de Pholiota adiposa. Se midió el rendimiento de sacarificación a diferentes pH de reacción de 1, 3, 5, 7 y 9. Se muestra el resultado en la Tabla 6.
Tabla 6
pH
Rendimiento de sacarificación (%)
1
20,0
3
62,6
5
81,4
7
84,0
9
39,4
15 Tal como se observa a partir de la Tabla 6, se obtuvo un rendimiento de sacarificación superior con pH 4-7. El mejor rendimiento de sacarificación se obtuvo con pH 7.
Ejemplo 6. Sacarificación en condiciones optimizadas
(1) Sacarificación de álamo mediante el uso de celulasa de Pholiota adiposa
Se llevó a cabo la sacarificación de álamo en condiciones optimizadas mediante el uso de celulasa producida por la
20 cepa SKU714 de Pholiota adiposa. La sacarificación se llevó a cabo en condiciones de una concentración de sustrato de 10% en peso, concentración de enzima de 25 FPU/g de sustrato, pH 6 y temperatura de 65°C. El rendimiento de sacarificación de la celulasa producida por la cepa SKU714 de Pholiota adiposa se comparó con el de la celulasa de Novozymes derivada de Trichoderma reesei (1,5 L de Celluclast) en la Tabla 7.
Tabla 7
Celulasa
Producción de azúcar (mg/g de álamo) Rendimiento de sacarificación (%)
SKU714 de Pholiota adiposa
672 84
1,5 L de Celluclast
242 35
imagen6
La presente Autoridad Depositaria Internacional acepta el microorganismo identificado anteriormente en I, el cual fue recibido el 20 de abril de 2011.
IV. AUTORIDAD DEPOSITARIA INTERNACIONAL
Nombre: Centro Coreano de Cultivo de Microorganismos Dirección: 361-221, Yurirn B/D Hongje-l-dong, Seodaemun-gu SEÚL 120-091 República de Corea
Firma/s de la/s persona/s autorizada/s a representar a la Autoridad Depositaria Internacional o del/de los funcionario/s autorizado/s Fecha: 20 de abril de 2011.
Formulario BP/4 (KCTC Formulario17)
Texto Libre
<110> SK CHEMICALS CO., LTD.
5
<120> Nueva cepa que produce celulasa y método de sacarificación que la utiliza
<130< DP-2011-0069 10 <161> 1
<170> KopatentIn 2.0
<210> 1 15 <211> 592
<212> ADN
<213> Pholiota adiposa
<400> 1
20
imagen7
<110> SK CHEMICALS CO., LTD. 5 <120> Nueva cepa que produce celulasa y método de sacarificación que la utiliza
<130> DP-2011-0069
<160> 1
10
<170> KopatentIn 2.0
<210> 1
<211> 592 15 <212> ADN
<213> Pholiota adiposa
<400> 1
imagen8

Claims (1)

  1. imagen1
ES13752441.9T 2012-02-24 2013-02-21 Nueva cepa fúngica para la producción de celulasa y método de sacarificación que la utiliza Active ES2618730T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
KR20120019335 2012-02-24
KR20120019335A KR20130097582A (ko) 2012-02-24 2012-02-24 셀룰라아제를 생산하는 신균주 및 이를 이용한 당화방법
PCT/KR2013/001406 WO2013125886A1 (ko) 2012-02-24 2013-02-21 셀룰라아제를 생산하는 신균주 및 이를 이용한 당화방법

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ES2618730T3 true ES2618730T3 (es) 2017-06-22

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US (2) US20150203808A1 (es)
EP (1) EP2829173B1 (es)
JP (1) JP6022606B2 (es)
KR (1) KR20130097582A (es)
CN (1) CN104254243B (es)
ES (1) ES2618730T3 (es)
WO (1) WO2013125886A1 (es)

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KR20150117599A (ko) 2014-04-09 2015-10-20 에스케이이노베이션 주식회사 바이오매스의 연속당화 반응기
KR101883628B1 (ko) * 2018-06-28 2018-08-30 건국대학교 산학협력단 2차 바이오매스 기질을 이용한 라카아제의 무독화 활성 증진 방법
CN109439574B (zh) * 2018-11-08 2022-09-02 唐山师范学院 纤维素酶产生菌及其应用
CN113637594B (zh) * 2021-10-18 2022-01-07 山东蓬勃生物科技有限公司 一种多脂鳞伞菌株yx1、培养方法及其应用
JP2025076528A (ja) * 2022-03-29 2025-05-16 三菱ケミカル株式会社 ハロゲン化炭化水素系樹脂の分解方法及びハロゲン化炭化水素系樹脂分解能を有する微生物
CN115181676B (zh) * 2022-07-01 2023-09-01 无锡赞匠生物科技有限公司 一株通过紫外诱变获得的黄伞菌株及其应用
CN115261238A (zh) * 2022-07-14 2022-11-01 大理大学 一种高产纤维素酶的菌株的发酵方法
CN116334111B (zh) * 2023-03-03 2024-02-09 上海市农业科学院 一种草菇纤维二糖水解酶及其用途

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CN101735952B (zh) * 2008-11-04 2011-09-21 中国农业科学院农业资源与农业区划研究所 黄伞新菌株
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KR20130097582A (ko) 2013-09-03
US20150203808A1 (en) 2015-07-23
JP2015508660A (ja) 2015-03-23
JP6022606B2 (ja) 2016-11-09
CN104254243B (zh) 2017-04-26
US10196661B2 (en) 2019-02-05
EP2829173A4 (en) 2015-10-28
EP2829173B1 (en) 2016-12-28
WO2013125886A1 (ko) 2013-08-29
CN104254243A (zh) 2014-12-31
EP2829173A1 (en) 2015-01-28
US20170226548A1 (en) 2017-08-10

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