ES2626848T3 - Dispositivos y métodos para la separación de partículas basada en la forma - Google Patents

Dispositivos y métodos para la separación de partículas basada en la forma Download PDF

Info

Publication number
ES2626848T3
ES2626848T3 ES12856833.4T ES12856833T ES2626848T3 ES 2626848 T3 ES2626848 T3 ES 2626848T3 ES 12856833 T ES12856833 T ES 12856833T ES 2626848 T3 ES2626848 T3 ES 2626848T3
Authority
ES
Spain
Prior art keywords
microchannel
outputs
shape
methods
devices
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES12856833.4T
Other languages
English (en)
Inventor
Dino Di Carlo
Mahdokht MASAELI
Elodie Sollier
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of California Berkeley
University of California San Diego UCSD
Original Assignee
University of California Berkeley
University of California San Diego UCSD
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of California Berkeley, University of California San Diego UCSD filed Critical University of California Berkeley
Application granted granted Critical
Publication of ES2626848T3 publication Critical patent/ES2626848T3/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03B—SEPARATING SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS
    • B03B5/00—Washing granular, powdered or lumpy materials; Wet separating
    • B03B5/48—Washing granular, powdered or lumpy materials; Wet separating by mechanical classifiers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502746—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means for controlling flow resistance, e.g. flow controllers, baffles or throttle valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/02—Investigating particle size or size distribution
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/08—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0636—Focussing flows, e.g. to laminate flows
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0652—Sorting or classification of particles or molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/088—Channel loops
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/02—Investigating particle size or size distribution
    • G01N2015/0288—Sorting the particles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/02—Investigating particle size or size distribution
    • G01N2015/0294—Particle shape

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Dispersion Chemistry (AREA)
  • Pathology (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Clinical Laboratory Science (AREA)
  • Hematology (AREA)
  • Fluid Mechanics (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Separation Of Solids By Using Liquids Or Pneumatic Power (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Un sistema de clasificación de partículas, que comprende: una entrada; un microcanal (14) de concentración inercial dispuesto en un sustrato y que tiene una región (18) que se expande aguas abajo en un extremo distal, en donde la entrada (12) se conecta a un extremo aguas arriba del microcanal; una fuente (30) de partículas con formas diferentes conectada a la entrada, en la que la fuente de partículas con formas diferentes se configuran para su introducción continua en la entrada; una pluralidad de salidas (20) conectada al microcanal en la región que se expande aguas abajo; caracterizado porque el sistema comprende además un controlador de presión conectado a la pluralidad de salidas por las líneas de fluidos, en donde el controlador de presión (23) ejerce presiones variables para ajustar la resistencia respectiva del fluido en la pluralidad de salidas.

Description

imagen1
imagen2
imagen3
imagen4
imagen5
imagen6
imagen7
imagen8
imagen9
etapas de crecimiento celular, en las que por ejemplo, las bacterias con forma de vara pueden tener hasta el doble de su longitud.
La separación basada en la forma usando efectos inerciales también puede usarse para la clasificación de células
5 de levadura y la sincronización del ciclo celular. La comprensión del ciclo celular es el sujeto de la investigación actual, que a menudo explora el uso de células de levadura (S. Cerevisiae) debido a la genética bien conocida y a los cambios de forma característicos); las células de levadura germinativas se alargan a partir de una esfera a un gemelo biesférico o a un agregado mayor. Usando el sistema de clasificación de partículas de la FIG. 7C (RAc = 0,64, siete salidas con α1:2 = 3/4, α1:3 = 1/2, α1:4 = 1/4), la clasificación de levaduras se llevó a cabo a un caudal de 60
10 μl/min. La levadura se cultivó en caldo de soja tríptico (CST) en un agitador incubado (37 ºC) durante un día antes del experimento de separación. La suspensión se cultivó se diluyó en TFS a una concentración no limitante de 1,5x106 células/ml y luego, de manera similar a las perlas, se inyectó a diversos caudales usando una bomba de jeringa Harvard Apparatus y una jeringa de vidrio Hamilton. El comportamiento de separación se capturó a través de la formación de imágenes a alta velocidad, siendo el contenido de cada salida analizado mediante el recuento
15 inmediato con un hemocitómetro (Quick-Read). Se observaron las morfologías de las células de levadura y se categorizaron, en función de su estado de ciclo, en (i) individuales pequeñas que no se dividen, (II) individuales grandes, (iii) levadura germinativa, (IV) como dobletes, y (v) agregados que se componen de tres o más células.
La FIG. 9A ilustra una imagen microscópica de las células en la entrada del dispositivo. Las células se categorizan
20 en cinco grupos: individuales pequeñas (recuadro superior), individuales grandes (segundo a partir del recuadro superior), germinativas (tercero a partir del recuadro superior), como dobletes (cuarto a partir del recuadro superior) y agregados (último recuadro). La FIG. 9B ilustra imágenes respectivas de la salida 2 y la salida 3. Las individuales tenían un alto rendimiento de extracción en la salida 2, mientras que en la salida 3, la pureza de las células germinativas aumentó.
25 Se descubrió que las individuales que no se dividen tenían un alto rendimiento de extracción en las salidas 2 y 6 (90 % de las individuales pequeñas y 91 % de las individuales grandes se recuperan en estas salidas como se aprecia en la FIG. 9C), con una pureza de hasta el 94 % (FIG. 9D), mientras que las células de levadura germinativas se recogieron principalmente en las salidas 3 y 5 (54 % de levadura germinativa, con una pureza de
30 hasta el 31 %, en comparación con una pureza del 6,6 % en la entrada). La mayor producción del sistema de clasificación de partículas (60 µl/min, es decir, 1.500 células/s en comparación con 100 células/s) se podría aumentar aún más por la paralelización de los canales de concentración, mientras que la pureza y enriquecimiento especialmente necesarios para esta aplicación de sincronización mejora con la conexión en cascada de varios dispositivos en serie.
35 En los experimentos se descubrió que un sistema de clasificación de partículas con RAc = 0,53 (An = 25 μm, Al = 47 μm) en Q = 40 µl/min que tiene cinco (5) salidas con resistencias iguales es el mejor dispositivo para separar varas largas (1:5) de 6 µm a partir de esferas y varas cortas (1:3), mientras que la separación de esferas de 6 µm de los dos tipos de varas se realiza mejor usando RAc = 0,64 (An = 30 µm, Al = 47 μm) en Q = 80 µl/min con cinco (5)
40 salidas con α1:2 = 3/4 y α1:3 = 1/2. El mejor dispositivo para la separación de los tres tipos de partículas de 6 µm era RAc = 0,64 (An = 30 µm, Al = 47 μm), en Q = 70 µl/min con siete (7) salidas con α1:2 = 3/4, α1:3 = 1/2, α1:4 = 1/4. Para esferas de partículas de 3 μm podría separarse mejor de los dos tipos de varas con RAc = 0,53 (An = 25 μm, Al = 47 μm) del dispositivo en Q = 80 µl/min con cinco (5) salidas con α1:2 = % y α1:3 = 1/2. El enriquecimiento de la levadura germinativa de la población total de células fue exitoso usando un dispositivo con RAc = 0,64 (An = 30 µm, Al = 47
45 μm) con siete (7) salidas con α1:2 = 3/4, α1 3 = 1/2, α1:4 = ¼. Las condiciones pueden ser optimizadas para otros modos de separación deseados, tales como el enriquecimiento de individuales, etc.
11

Claims (1)

  1. imagen1
    imagen2
ES12856833.4T 2011-09-30 2012-09-27 Dispositivos y métodos para la separación de partículas basada en la forma Active ES2626848T3 (es)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201161541934P 2011-09-30 2011-09-30
US201161541934P 2011-09-30
US201261606287P 2012-03-02 2012-03-02
US201261606287P 2012-03-02
PCT/US2012/057631 WO2013089883A2 (en) 2011-09-30 2012-09-27 Devices and methods for shape-based particle separation

Publications (1)

Publication Number Publication Date
ES2626848T3 true ES2626848T3 (es) 2017-07-26

Family

ID=48613336

Family Applications (1)

Application Number Title Priority Date Filing Date
ES12856833.4T Active ES2626848T3 (es) 2011-09-30 2012-09-27 Dispositivos y métodos para la separación de partículas basada en la forma

Country Status (9)

Country Link
US (1) US9333510B2 (es)
EP (1) EP2761303B1 (es)
JP (1) JP6031109B2 (es)
KR (1) KR20140084075A (es)
CN (1) CN103959069B (es)
AU (1) AU2012352879B2 (es)
CA (1) CA2850335A1 (es)
ES (1) ES2626848T3 (es)
WO (1) WO2013089883A2 (es)

Families Citing this family (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11243494B2 (en) 2002-07-31 2022-02-08 Abs Global, Inc. Multiple laminar flow-based particle and cellular separation with laser steering
US10908066B2 (en) 2010-11-16 2021-02-02 1087 Systems, Inc. Use of vibrational spectroscopy for microfluidic liquid measurement
US8961904B2 (en) 2013-07-16 2015-02-24 Premium Genetics (Uk) Ltd. Microfluidic chip
US11796449B2 (en) 2013-10-30 2023-10-24 Abs Global, Inc. Microfluidic system and method with focused energy apparatus
WO2016010861A1 (en) * 2014-07-14 2016-01-21 President And Fellows Of Harvard College Systems and methods for improved performance of fluidic and microfluidic systems
US10180388B2 (en) 2015-02-19 2019-01-15 1087 Systems, Inc. Scanning infrared measurement system
US10591403B2 (en) 2016-03-16 2020-03-17 Georgia Tech Research Corporation Multiplexed analysis of cell-materials in niches
US12128407B2 (en) * 2016-04-15 2024-10-29 Vortex Biosciences, Inc. Microfluidic chips and cartridges and systems utilizing microfluidic chips and cartridges
US10471425B2 (en) 2017-02-16 2019-11-12 International Business Machines Corporation Automated machine for sorting of biological fluids
US11326991B2 (en) * 2017-11-14 2022-05-10 Aerodyne Microsystems Inc. Airborne particle detection with selective thermophoretic particle deflection
CN108405169B (zh) * 2018-03-07 2020-03-10 中国工程物理研究院激光聚变研究中心 一种液体筛分微球装置
US11229912B2 (en) 2018-03-27 2022-01-25 Hewlett-Packard Development Company, L.P. Particle separation
SG11202010398RA (en) * 2018-05-09 2020-11-27 Hoffmann La Roche Devices and methods for determining particle concentration in a sample
US11331670B2 (en) 2018-05-23 2022-05-17 Abs Global, Inc. Systems and methods for particle focusing in microchannels
CN108872047B (zh) * 2018-08-15 2021-02-26 军事科学院系统工程研究院卫勤保障技术研究所 一种微流控电阻抗检测区分微小粒子形状的系统及方法
US10611995B2 (en) 2018-08-15 2020-04-07 Deepcell, Inc. Systems and methods for particle analysis
US11815507B2 (en) 2018-08-15 2023-11-14 Deepcell, Inc. Systems and methods for particle analysis
US20200070167A1 (en) * 2018-08-31 2020-03-05 Vortex Biosciences, Inc. Processing systems for isolating and enumerating cells or particles
EP3899489B1 (en) 2018-12-21 2025-04-09 ABS Global, Inc. System and methods for sub-population identification
US11524292B2 (en) * 2019-02-21 2022-12-13 International Business Machines Corporation Programmable hydraulic resistor array for microfluidic chips
EP3955735B1 (en) 2019-04-18 2024-05-22 ABS Global, Inc. System and process for continuous addition of cryoprotectant
US12053569B2 (en) 2019-09-04 2024-08-06 The Regents Of The University Of Michigan Indwelling intravascular aphaeretic system for in vivo enrichment of circulating tumor cells
CN114901392B (zh) * 2019-10-21 2024-10-29 南洋理工大学 使用离心力从全血中直接和可扩展地分离循环胞外囊泡
US11628439B2 (en) * 2020-01-13 2023-04-18 Abs Global, Inc. Single-sheath microfluidic chip
WO2022087299A1 (en) 2020-10-21 2022-04-28 Abs Global, Inc. Methods and systems for processing genetic samples to determine identity or detect contamination
US12135270B2 (en) 2020-11-23 2024-11-05 Abs Global, Inc. Modular flow cytometry systems and methods of processing samples
WO2022170340A1 (en) * 2021-02-03 2022-08-11 University Of Georgia Research Foundation, Inc. Devices, kits, and methods for label-free inertial ferrohydrodynamic cell separation with high throughput and resolution
CN112973815B (zh) * 2021-03-09 2022-06-03 西北大学 一种聚集非球形微游泳体的微流管道及一种过滤方法
US12576194B2 (en) 2021-06-29 2026-03-17 Regents Of The University Of Michigan Microfluidic flow control using direct-current peristaltic pump
CN113769797B (zh) * 2021-09-02 2023-03-14 浙江理工大学 一种流固两相输运中的微尺度颗粒直径测定的方法
USD1118965S1 (en) 2021-11-12 2026-03-17 Abs Global, Inc. Flow cytometry device
CN118067593A (zh) * 2022-11-22 2024-05-24 中国科学院深圳先进技术研究院 微流控分选芯片、控制设备、微流控分选系统及方法
CN116673080B (zh) * 2023-06-27 2025-09-02 深圳大学 微流控制装置的制备方法及黏弹性-惯性微流控分选装置
CN119738236A (zh) * 2024-12-30 2025-04-01 上海交通大学 一种生物检测芯片、检测方法及检测设备

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7735652B2 (en) * 2006-06-01 2010-06-15 The Trustees Of Princeton University Apparatus and method for continuous particle separation
US8186913B2 (en) * 2007-04-16 2012-05-29 The General Hospital Corporation Systems and methods for particle focusing in microchannels
JP5304506B2 (ja) * 2009-07-22 2013-10-02 富士ゼロックス株式会社 分級装置及び分級方法
SG183468A1 (en) 2010-03-04 2012-09-27 Univ Singapore Microfluidics sorter for cell detection and isolation
US8590710B2 (en) * 2010-06-10 2013-11-26 Samsung Electronics Co., Ltd. Target particles-separating device and method using multi-orifice flow fractionation channel
US9090865B2 (en) * 2010-10-29 2015-07-28 The Regents Of The University Of California Systems and methods for particle classification and sorting

Also Published As

Publication number Publication date
EP2761303A2 (en) 2014-08-06
WO2013089883A3 (en) 2013-08-29
AU2012352879A1 (en) 2014-04-10
EP2761303A4 (en) 2015-06-03
JP6031109B2 (ja) 2016-11-24
WO2013089883A2 (en) 2013-06-20
CN103959069B (zh) 2016-02-24
CA2850335A1 (en) 2013-06-20
KR20140084075A (ko) 2014-07-04
CN103959069A (zh) 2014-07-30
JP2015501136A (ja) 2015-01-15
US9333510B2 (en) 2016-05-10
US20140224710A1 (en) 2014-08-14
AU2012352879B2 (en) 2017-01-05
EP2761303B1 (en) 2017-03-01

Similar Documents

Publication Publication Date Title
ES2626848T3 (es) Dispositivos y métodos para la separación de partículas basada en la forma
Wu et al. Microfluidic technology for the isolation and analysis of exosomes
Li et al. Size-based sorting of hydrogel droplets using inertial microfluidics
Bagi et al. Advances in technical assessment of spiral inertial microfluidic devices toward bioparticle separation and profiling: A critical review
CN104877898B (zh) 一种低成本、高效分离获取单细胞的系统和方法
WO2014145152A3 (en) On-chip microfluidic processing of particles
Wang et al. High‐throughput inertial focusing of micrometer‐and sub‐micrometer‐sized particles separation
CN109580323B (zh) 一种螺旋形微通道及其使用方法与串、并联安装结构
CN101765762A (zh) 使粒子在微通道中聚集的系统和方法
CN107702973A (zh) 一种全血血浆分离系统及方法
CN103923816B (zh) 一种基于微流控技术的细胞捕获阵列
US20210053061A1 (en) Inertial cell focusing and sorting
TW201902555A (zh) 用於灌注應用之切向流過濾裝置
CN207366327U (zh) 一种全血血浆分离系统
Sherbaz et al. Deterministic lateral displacement microfluidic chip for minicell purification
Wang et al. The automatic and high‐throughput purification and enrichment of microalgae cells using deterministic lateral displacement arrays with different post shapes
CN114574357B (zh) 一种基于确定性侧向位移和介电泳技术联用的微藻与细菌分选装置
Wang et al. High-throughput microalgae sorting based on the deterministic lateral displacement technique
CN102896006B (zh) 微球分离筛选芯片及其制备方法
Qian et al. Coupling inertial effects and deterministic lateral displacement for high-purity microalgae separation in a microfluidic platform
CN202786219U (zh) 微流控生物芯片
CN108949497A (zh) 极微量循环肿瘤细胞的特异性单细胞定点捕捉芯片
CN204503113U (zh) 多级微球筛选芯片
CN103421082B (zh) 一种开放式恒流扩散蛋白质结晶阵列芯片及其制作方法
Ma et al. Experimental and theoretical study of hydrodynamic cell lysing of cancer cells in a high-throughput Circular Multi-Channel Microfiltration device