ES2627792T3 - O-acetilhomoserina sulfhidrilasa novedosa o variante de la misma y procedimiento para transformar la metionina usando la misma - Google Patents

O-acetilhomoserina sulfhidrilasa novedosa o variante de la misma y procedimiento para transformar la metionina usando la misma Download PDF

Info

Publication number: ES2627792T3
Authority: ES; Spain
Prior art keywords: reaction; methionine; acetylhomoserine; metz; rsp
Prior art date: 2010-12-21
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Active

Application number

ES11850058.6T

Other languages

English (en)

Spanish (es)

Inventor

So Young Kim

Yong Uk Shin

Chang Il Seo

Sung Kwang Son

In Kyung Heo

Han Jin Lee

Ju Eun Kim

Hyun Ah Kim

Jee Yeon BAE

Kwang Ho Na

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

CJ CheilJedang Corp

Original Assignee

CJ CheilJedang Corp

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2010-12-21

Filing date

2011-12-21

Publication date

2017-07-31

2011-12-21 Application filed by CJ CheilJedang Corp filed Critical CJ CheilJedang Corp

2017-07-31 Application granted granted Critical

2017-07-31 Publication of ES2627792T3 publication Critical patent/ES2627792T3/es

Status Active legal-status Critical Current

2031-12-21 Anticipated expiration legal-status Critical

Links

FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 title description 20
229930182817 methionine Natural products 0.000 title description 15
101710083973 Homocysteine synthase Proteins 0.000 title description 12
238000000034 method Methods 0.000 title description 5
230000001131 transforming effect Effects 0.000 title 1
FCXZBWSIAGGPCB-YFKPBYRVSA-N O-acetyl-L-homoserine Chemical compound CC(=O)OCC[C@H]([NH3+])C([O-])=O FCXZBWSIAGGPCB-YFKPBYRVSA-N 0.000 abstract description 20
108090000623 proteins and genes Proteins 0.000 abstract description 4
102000004169 proteins and genes Human genes 0.000 abstract description 3
125000003275 alpha amino acid group Chemical group 0.000 abstract 1
238000006243 chemical reaction Methods 0.000 description 41
230000035772 mutation Effects 0.000 description 24
LSDPWZHWYPCBBB-UHFFFAOYSA-N Methanethiol Chemical compound SC LSDPWZHWYPCBBB-UHFFFAOYSA-N 0.000 description 22
229960004452 methionine Drugs 0.000 description 19
102000004190 Enzymes Human genes 0.000 description 14
108090000790 Enzymes Proteins 0.000 description 14
239000011541 reaction mixture Substances 0.000 description 14
230000000694 effects Effects 0.000 description 13
230000002255 enzymatic effect Effects 0.000 description 13
239000000758 substrate Substances 0.000 description 10
KIUMMUBSPKGMOY-UHFFFAOYSA-N 3,3'-Dithiobis(6-nitrobenzoic acid) Chemical compound C1=C([N+]([O-])=O)C(C(=O)O)=CC(SSC=2C=C(C(=CC=2)[N+]([O-])=O)C(O)=O)=C1 KIUMMUBSPKGMOY-UHFFFAOYSA-N 0.000 description 8
239000002609 medium Substances 0.000 description 7
XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 description 5
FFEARJCKVFRZRR-UHFFFAOYSA-N L-Methionine Natural products CSCCC(N)C(O)=O FFEARJCKVFRZRR-UHFFFAOYSA-N 0.000 description 5
229930195722 L-methionine Natural products 0.000 description 5
241000191043 Rhodobacter sphaeroides Species 0.000 description 5
241001619535 Hyphomonas neptunium Species 0.000 description 4
102220465273 Interferon alpha-8_F65Y_mutation Human genes 0.000 description 4
GNISQJGXJIDKDJ-YFKPBYRVSA-N O-succinyl-L-homoserine Chemical compound OC(=O)[C@@H](N)CCOC(=O)CCC(O)=O GNISQJGXJIDKDJ-YFKPBYRVSA-N 0.000 description 4
XHXXWWGGXFUMAJ-UHFFFAOYSA-N methanethiol;sodium Chemical compound [Na].SC XHXXWWGGXFUMAJ-UHFFFAOYSA-N 0.000 description 4
NGVDGCNFYWLIFO-UHFFFAOYSA-N pyridoxal 5'-phosphate Chemical compound CC1=NC=C(COP(O)(O)=O)C(C=O)=C1O NGVDGCNFYWLIFO-UHFFFAOYSA-N 0.000 description 4
235000007682 pyridoxal 5'-phosphate Nutrition 0.000 description 4
239000011589 pyridoxal 5'-phosphate Substances 0.000 description 4
239000013598 vector Substances 0.000 description 4
QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
238000004458 analytical method Methods 0.000 description 3
238000002474 experimental method Methods 0.000 description 3
238000000855 fermentation Methods 0.000 description 3
230000004151 fermentation Effects 0.000 description 3
238000010438 heat treatment Methods 0.000 description 3
238000004128 high performance liquid chromatography Methods 0.000 description 3
230000000813 microbial effect Effects 0.000 description 3
238000012163 sequencing technique Methods 0.000 description 3
238000003756 stirring Methods 0.000 description 3
QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
241001646716 Escherichia coli K-12 Species 0.000 description 2
235000013878 L-cysteine Nutrition 0.000 description 2
239000004201 L-cysteine Substances 0.000 description 2
RTAQQCXQSZGOHL-UHFFFAOYSA-N Titanium Chemical compound [Ti] RTAQQCXQSZGOHL-UHFFFAOYSA-N 0.000 description 2
238000002835 absorbance Methods 0.000 description 2
230000015572 biosynthetic process Effects 0.000 description 2
HAAZLUGHYHWQIW-KVQBGUIXSA-N dGTP Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HAAZLUGHYHWQIW-KVQBGUIXSA-N 0.000 description 2
239000000284 extract Substances 0.000 description 2
229910000357 manganese(II) sulfate Inorganic materials 0.000 description 2
235000018102 proteins Nutrition 0.000 description 2
238000012216 screening Methods 0.000 description 2
239000000126 substance Substances 0.000 description 2
239000006228 supernatant Substances 0.000 description 2
229910052719 titanium Inorganic materials 0.000 description 2
239000010936 titanium Substances 0.000 description 2
NNEBIZZUFUWQDP-VDQHJUMDSA-N (2s)-4-acetyloxy-2-aminobutanoic acid;(2s)-2-amino-4-methylsulfanylbutanoic acid Chemical compound CSCC[C@H](N)C(O)=O.CC(=O)OCC[C@H](N)C(O)=O NNEBIZZUFUWQDP-VDQHJUMDSA-N 0.000 description 1
UKAUYVFTDYCKQA-UHFFFAOYSA-N -2-Amino-4-hydroxybutanoic acid Natural products OC(=O)C(N)CCO UKAUYVFTDYCKQA-UHFFFAOYSA-N 0.000 description 1
UKAUYVFTDYCKQA-VKHMYHEASA-N L-homoserine Chemical compound OC(=O)[C@@H](N)CCO UKAUYVFTDYCKQA-VKHMYHEASA-N 0.000 description 1
AFCARXCZXQIEQB-UHFFFAOYSA-N N-[3-oxo-3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propyl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(CCNC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 AFCARXCZXQIEQB-UHFFFAOYSA-N 0.000 description 1
108010006785 Taq Polymerase Proteins 0.000 description 1
230000003190 augmentative effect Effects 0.000 description 1
239000000872 buffer Substances 0.000 description 1
238000012258 culturing Methods 0.000 description 1
XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 1
235000018417 cysteine Nutrition 0.000 description 1
238000010586 diagram Methods 0.000 description 1
230000008034 disappearance Effects 0.000 description 1
238000006911 enzymatic reaction Methods 0.000 description 1
238000011156 evaluation Methods 0.000 description 1
239000013604 expression vector Substances 0.000 description 1
239000001963 growth medium Substances 0.000 description 1
239000007788 liquid Substances 0.000 description 1
SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 description 1
238000004519 manufacturing process Methods 0.000 description 1
239000000203 mixture Substances 0.000 description 1
238000002703 mutagenesis Methods 0.000 description 1
231100000350 mutagenesis Toxicity 0.000 description 1
239000002773 nucleotide Substances 0.000 description 1
125000003729 nucleotide group Chemical group 0.000 description 1
239000008057 potassium phosphate buffer Substances 0.000 description 1
239000000047 product Substances 0.000 description 1
238000002708 random mutagenesis Methods 0.000 description 1
239000011734 sodium Substances 0.000 description 1
235000011121 sodium hydroxide Nutrition 0.000 description 1
238000003786 synthesis reaction Methods 0.000 description 1
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/12—Methionine; Cysteine; Cystine
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/54—Acetic acid
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y205/00—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5)
- C12Y205/01—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5) transferring alkyl or aryl groups, other than methyl groups (2.5.1)
- C12Y205/01049—O-acetylhomoserine aminocarboxypropyltransferase (2.5.1.49)

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Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Life Sciences & Earth Sciences (AREA)
Engineering & Computer Science (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Bioinformatics & Cheminformatics (AREA)
General Health & Medical Sciences (AREA)
Biochemistry (AREA)
General Engineering & Computer Science (AREA)
Microbiology (AREA)
Biotechnology (AREA)
Chemical Kinetics & Catalysis (AREA)
General Chemical & Material Sciences (AREA)
Biomedical Technology (AREA)
Molecular Biology (AREA)
Medicinal Chemistry (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Enzymes And Modification Thereof (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)

ES11850058.6T 2010-12-21 2011-12-21 O-acetilhomoserina sulfhidrilasa novedosa o variante de la misma y procedimiento para transformar la metionina usando la misma Active ES2627792T3 (es)

Applications Claiming Priority (3)

Application Number	Priority Date	Filing Date	Title
KR1020100131954A KR101250651B1 (ko)	2010-12-21	2010-12-21	신규 ｏ-아세틸호모세린 설피드릴라제 또는 변이체 및 이를 이용한 메치오닌 전환 방법
KR20100131954		2010-12-21
PCT/KR2011/009965 WO2012087038A2 (ko)	2010-12-21	2011-12-21	신규 ｏ-아세틸호모세린 설피드릴라아제 또는 이의 변이체 및 이를 이용한 메치오닌 전환 방법

Publications (1)

Publication Number	Publication Date
ES2627792T3 true ES2627792T3 (es)	2017-07-31

Family

ID=46314641

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
ES11850058.6T Active ES2627792T3 (es)	2010-12-21	2011-12-21	O-acetilhomoserina sulfhidrilasa novedosa o variante de la misma y procedimiento para transformar la metionina usando la misma

Country Status (11)

Country	Link
US (1)	US9080155B2 (pl)
EP (1)	EP2657249B1 (pl)
JP (1)	JP5907993B2 (pl)
KR (1)	KR101250651B1 (pl)
CN (1)	CN103328498B (pl)
BR (1)	BR112013015992B1 (pl)
DK (1)	DK2657249T3 (pl)
ES (1)	ES2627792T3 (pl)
PL (1)	PL2657249T3 (pl)
RU (1)	RU2573928C2 (pl)
WO (1)	WO2012087038A2 (pl)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
CN108342424A (zh) *	2011-09-02	2018-07-31	阿克马法国公司	L-甲硫氨酸的制备方法
ES2906229T3 (es)	2015-06-04	2022-04-13	Cj Cheiljedang Corp	Microorganismo productor de O-acetil-homoserina, y método para producir O-acetil-homoserina mediante el uso de este
WO2017009047A1 (en) *	2015-07-13	2017-01-19	Evonik Degussa Gmbh	Method for producing l-methionine
MY186559A (en) *	2015-10-13	2021-07-27	Cj Cheiljedang Corp	O-acetylhomoserine sulfhydrylase variant and method for producing l-methionine using same
KR102016050B1 (ko)	2018-03-20	2019-08-29	씨제이제일제당 주식회사	신규한 프로모터 및 이의 용도
KR102377500B1 (ko) *	2019-10-28	2022-03-23	씨제이제일제당 주식회사	외래 metZ 유전자에 의해 코딩되는 단백질이 도입된 L-메티오닌 생산 미생물 및 이를 이용한 L-메티오닌 생산방법
JP2026503321A (ja) *	2023-01-31	2026-01-28	シージェイチェイルジェダンコーポレーション	Ｏ－アシルホモセリンスルフヒドリラーゼ変異体及びその用途
CN120843473A (zh) *	2025-09-25	2025-10-28	山东天力药业有限公司	一种o-乙酰高丝氨酸硫化氢解酶突变体、表达该突变体的重组工程菌及其应用

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JP4110641B2 (ja) *	1998-11-17	2008-07-02	味の素株式会社	発酵法によるｌ−メチオニンの製造法
DE10109690A1 (de) *	2000-09-02	2002-03-14	Degussa	Neue für das metY-Gen kodierende Nukleotidsequenzen
AU2004245988A1 (en)	2003-05-30	2004-12-16	Microbia, Inc.	Methods and compositions for amino acid production
KR100620092B1 (ko)	2004-12-16	2006-09-08	씨제이 주식회사	코리네박테리움 속 세포로부터 유래된 신규한 프로모터서열, 그를 포함하는 발현 카세트 및 벡터, 상기 벡터를포함하는 숙주 세포 및 그를 이용하여 유전자를 발현하는방법
RU2413001C2 (ru) *	2005-07-18	2011-02-27	Эвоник Дегусса Гмбх	Применение диметилдисульфида для продукции метионина микроорганизмами
KR100905381B1 (ko) *	2006-07-28	2009-06-30	씨제이제일제당 (주)	Ｌ-메치오닌 전구체 생산 균주 및 상기 ｌ-메치오닌전구체로부터의 ｌ-메치오닌 및 유기산의 생산방법
US7851180B2 (en)	2008-04-04	2010-12-14	Cj Cheiljedang Corporation	Microorganism producing L-methionine precursor and the method of producing L-methionine precursor using the microorganism
KR101136248B1 (ko) *	2008-04-04	2012-04-20	씨제이제일제당 (주)	Ｌ-메치오닌 전구체 생산 균주 및 이를 이용한 ｌ-메치오닌 전구체의 생산 방법
KR101048593B1 (ko)	2009-02-27	2011-07-12	씨제이제일제당 (주)	메칠머캅탄과 디메칠설파이드의 혼합물을 사용하여 메치오닌 생산능을 증가시키는 방법

2010
- 2010-12-21 KR KR1020100131954A patent/KR101250651B1/ko active Active
2011
- 2011-12-21 DK DK11850058.6T patent/DK2657249T3/en active
- 2011-12-21 JP JP2013546020A patent/JP5907993B2/ja active Active
- 2011-12-21 PL PL11850058T patent/PL2657249T3/pl unknown
- 2011-12-21 BR BR112013015992-8A patent/BR112013015992B1/pt active IP Right Grant
- 2011-12-21 US US13/997,054 patent/US9080155B2/en active Active
- 2011-12-21 RU RU2013133636/10A patent/RU2573928C2/ru active
- 2011-12-21 CN CN201180065568.6A patent/CN103328498B/zh active Active
- 2011-12-21 ES ES11850058.6T patent/ES2627792T3/es active Active
- 2011-12-21 WO PCT/KR2011/009965 patent/WO2012087038A2/ko not_active Ceased
- 2011-12-21 EP EP11850058.6A patent/EP2657249B1/en active Active

Also Published As

Publication number	Publication date
WO2012087038A3 (ko)	2012-08-23
JP5907993B2 (ja)	2016-04-26
RU2013133636A (ru)	2015-01-27
US20130273614A1 (en)	2013-10-17
JP2014506129A (ja)	2014-03-13
RU2573928C2 (ru)	2016-01-27
US9080155B2 (en)	2015-07-14
BR112013015992A2 (pt)	2020-07-07
EP2657249B1 (en)	2017-03-15
DK2657249T3 (en)	2017-06-06
WO2012087038A2 (ko)	2012-06-28
CN103328498B (zh)	2015-11-25
CN103328498A (zh)	2013-09-25
KR20120070411A (ko)	2012-06-29
PL2657249T3 (pl)	2017-08-31
EP2657249A2 (en)	2013-10-30
BR112013015992B1 (pt)	2022-01-25
EP2657249A4 (en)	2014-10-01
KR101250651B1 (ko)	2013-04-03

Publication	Publication Date	Title
ES2627792T3 (es)	2017-07-31	O-acetilhomoserina sulfhidrilasa novedosa o variante de la misma y procedimiento para transformar la metionina usando la misma
ES2534375T3 (es)	2015-04-22	Composiciones y procedimientos de producción de metionina
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