ES2641014T3 - Gen Dro1 que controla características de enraizamiento profundo de planta y utilización del mismo - Google Patents

Gen Dro1 que controla características de enraizamiento profundo de planta y utilización del mismo Download PDF

Info

Publication number
ES2641014T3
ES2641014T3 ES10839538.5T ES10839538T ES2641014T3 ES 2641014 T3 ES2641014 T3 ES 2641014T3 ES 10839538 T ES10839538 T ES 10839538T ES 2641014 T3 ES2641014 T3 ES 2641014T3
Authority
ES
Spain
Prior art keywords
dna
plant
seq
deep rooting
amino acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES10839538.5T
Other languages
English (en)
Inventor
Yusaku Uga
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
National Agriculture and Food Research Organization
Original Assignee
National Agriculture and Food Research Organization
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by National Agriculture and Food Research Organization filed Critical National Agriculture and Food Research Organization
Application granted granted Critical
Publication of ES2641014T3 publication Critical patent/ES2641014T3/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/8223—Vegetative tissue-specific promoters
    • C12N15/8227—Root-specific
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/52—Bacterial cells; Fungal cells; Protozoal cells
    • A61K2039/521—Bacterial cells; Fungal cells; Protozoal cells inactivated (killed)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2300/00—Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Immunology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • General Preparation And Processing Of Foods (AREA)

Abstract

Método para conferir un rasgo de enraizamiento profundo a una planta, que comprende las etapas de (i) y (ii) a continuación: (i) introducir en una célula vegetal un ADN de uno cualquiera de (a) a (e) a continuación: (a) un ADN que comprende la secuencia de nucleótidos de SEQ ID NO: 1; (b) un ADN que comprende una región codificante de la secuencia de nucleótidos de una cualquiera de SEQ ID NO: 1, 2, 12, 14, 16 y 17; (c) un ADN que codifica para una proteína que comprende la secuencia de aminoácidos de una cualquiera de SEQ ID NO: 3, 13 y 15; (d) un ADN que hibrida en condiciones rigurosas con un ADN que comprende la secuencia de nucleótidos de una cualquiera de SEQ ID NO: 1, 2, 12, 14, 16 y 17, y tiene una actividad de conferir un fenotipo de enraizamiento profundo a una planta; o (e) un ADN que codifica para una proteína que comprende una secuencia de aminoácidos con una o más sustituciones, deleciones, adiciones y/o inserciones de aminoácido en la secuencia de aminoácidos de una cualquiera de SEQ ID NO: 3, 13 y 15, y tiene una actividad de conferir un fenotipo de enraizamiento profundo a una planta o un vector que porta dicho ADN de uno cualquiera de (a) a (e); y (ii) regenerar una planta a partir de la célula vegetal a la que se le introdujo el ADN o el vector en la etapa (i).
ES10839538.5T 2009-12-24 2010-12-24 Gen Dro1 que controla características de enraizamiento profundo de planta y utilización del mismo Active ES2641014T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2009292524 2009-12-24
JP2009292524 2009-12-24
PCT/JP2010/073288 WO2011078308A1 (ja) 2009-12-24 2010-12-24 植物の深根性を制御する遺伝子Dro1とその利用

Publications (1)

Publication Number Publication Date
ES2641014T3 true ES2641014T3 (es) 2017-11-07

Family

ID=44195833

Family Applications (1)

Application Number Title Priority Date Filing Date
ES10839538.5T Active ES2641014T3 (es) 2009-12-24 2010-12-24 Gen Dro1 que controla características de enraizamiento profundo de planta y utilización del mismo

Country Status (10)

Country Link
US (2) US20120317678A1 (es)
EP (1) EP2518148B1 (es)
JP (1) JP5791049B2 (es)
KR (1) KR101806342B1 (es)
CN (1) CN102918154B (es)
AU (1) AU2010336253B2 (es)
BR (1) BR112012017055B1 (es)
CA (1) CA2782300C (es)
ES (1) ES2641014T3 (es)
WO (1) WO2011078308A1 (es)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105132440B (zh) * 2015-09-23 2018-08-21 安徽农业大学 与小麦叶片叶绿素含量和粒重相关的基因、其Indel标记及应用
US10513708B2 (en) * 2016-09-30 2019-12-24 The United States Of America, As Represented By The Secretary Of Agriculture DRO1 related genes influence lateral root orientation and growth in Arabidopsis and Prunus species
CN108411020B (zh) * 2018-02-06 2021-10-01 北京市农林科学院 适于毛细管电泳检测平台的一套玉米叶绿体InDel分子标记
CN110656125A (zh) * 2019-09-23 2020-01-07 四川育良生物科技有限公司 一种耐干旱玉米的遗传转化方法
GB201914137D0 (en) * 2019-10-01 2019-11-13 Univ Leeds Innovations Ltd Modified Plants
CN117203227A (zh) 2021-02-25 2023-12-08 成对植物服务股份有限公司 用于修饰植物中根结构的方法和组合物
CN112997879B (zh) * 2021-03-22 2023-01-31 甘肃农业大学 一种评价玉米中胚轴和胚芽鞘最大伸长特性的方法
JP7658590B2 (ja) * 2022-05-13 2025-04-08 国立研究開発法人農業・食品産業技術総合研究機構 水田からのメタン排出量の削減方法、イネにおけるメタン排出量の調節の程度を判定する判定方法、及びイネの包装品
CN114736280B (zh) * 2022-05-24 2023-03-24 中国农业大学 ZmROA1蛋白在调控植物耐密性中的应用
AR133164A1 (es) * 2023-07-18 2025-09-03 Pairwise Plants Services Inc Métodos y composiciones para modificar la arquitectura radicular en plantas
CN117447575B (zh) * 2023-12-19 2024-03-08 中国农业大学 深根蛋白在特异性调控玉米根夹角中的应用

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2649287B2 (ja) 1992-07-07 1997-09-03 日本たばこ産業株式会社 単子葉植物の形質転換方法
US7868149B2 (en) * 1999-07-20 2011-01-11 Monsanto Technology Llc Plant genome sequence and uses thereof
AU765277B2 (en) 1999-11-04 2003-09-11 National Agriculture And Bio-Oriented Research Organization Plant photoperiod sensitivity gene Hd1 and use of the same
US6936467B2 (en) * 2000-03-27 2005-08-30 University Of Delaware Targeted chromosomal genomic alterations with modified single stranded oligonucleotides
US20110131679A2 (en) * 2000-04-19 2011-06-02 Thomas La Rosa Rice Nucleic Acid Molecules and Other Molecules Associated with Plants and Uses Thereof for Plant Improvement
JP2002153283A (ja) 2000-11-24 2002-05-28 National Institute Of Agrobiological Sciences 植物の開花を誘導する遺伝子Ｈｄ３ａおよびその利用
JP3911202B2 (ja) 2002-05-28 2007-05-09 独立行政法人農業生物資源研究所 植物の開花時期を促進するＥｈｄ１遺伝子およびその利用
JP2005185101A (ja) * 2002-05-30 2005-07-14 National Institute Of Agrobiological Sciences 植物の全長ｃＤＮＡおよびその利用
JP3823137B2 (ja) 2002-08-30 2006-09-20 独立行政法人農業生物資源研究所 植物の開花促進遺伝子ｒｆｔ１および植物の開花時期を予測する方法
JP4504049B2 (ja) 2003-03-10 2010-07-14 独立行政法人農業生物資源研究所 植物の開花を制御する遺伝子Ｌｈｄ４とその利用
CN1486593A (zh) * 2003-07-30 2004-04-07 上海市农业生物基因中心 一种构建成水稻遗传测验材料的方法
JP4404341B2 (ja) 2003-10-08 2010-01-27 独立行政法人農業生物資源研究所 植物の感光性遺伝子Ｈｄ５およびその利用
CN1330755C (zh) * 2004-01-15 2007-08-08 向成斌 一种拟南芥转录因子及其编码基因与应用
CN1271916C (zh) * 2004-06-04 2006-08-30 广东省农业科学院作物研究所 一种节水抗旱的花生新品种的选育方法
WO2006123392A1 (ja) 2005-05-16 2006-11-23 Japan Tobacco Inc. 植物の深根性評価方法
JP2007222129A (ja) 2006-02-27 2007-09-06 Institute Of Physical & Chemical Research 環境ストレス耐性植物の作出方法

Also Published As

Publication number Publication date
AU2010336253B2 (en) 2014-09-18
BR112012017055A2 (pt) 2015-09-01
US20120317678A1 (en) 2012-12-13
EP2518148A1 (en) 2012-10-31
JPWO2011078308A1 (ja) 2013-05-09
KR101806342B1 (ko) 2017-12-07
EP2518148A4 (en) 2013-10-23
KR20120101542A (ko) 2012-09-13
WO2011078308A1 (ja) 2011-06-30
CN102918154B (zh) 2016-02-17
BR112012017055B1 (pt) 2019-02-26
EP2518148B1 (en) 2017-07-26
CA2782300A1 (en) 2011-06-30
CA2782300C (en) 2019-05-14
AU2010336253A1 (en) 2012-06-14
JP5791049B2 (ja) 2015-10-07
CN102918154A (zh) 2013-02-06
US20170306421A1 (en) 2017-10-26

Similar Documents

Publication Publication Date Title
MX339869B (es) Polinucleotidos y polipeptidos aislados, y metodos para utilizarlos para mejorar la eficacia en el uso de nitrogeno, rendimiento, tasa de crecimiento, vigor, biomasa, contenido de aceite y/o tolerancia al estres abiotico.
PH12014500455A1 (en) Isopropylmalate synthase from nicotiana tabacum and methods and uses thereof
MX2021012596A (es) Polinucleotidos y polipeptidos aislados, sus metodos de utilizacion para mejorar la eficacia en el uso del nitrogeno, rendimiento, tasa de crecimiento, vigor, biomasa, contenido de aceite y/o tolerancia al estres abiotico.
ES2565060T3 (es) Polipéptidos que tienen actividad de glucoamilasa y polinucleótidos que codifican los mismos
AR093346A2 (es) Construccion genetica que comprende una secuencia de un promotor de antiquitina de arroz y vector que la comprende
IN2012DN00403A (es)
MX2020005724A (es) Variantes de alfa-amilasa y polinucleotidos que codifican las mismas.
NZ604057A (en) Plant cytochrome p450
AR088263A1 (es) Moleculas de acido nucleico que se dirigen a rps6 y confieren resistencia a plagas de coleopteros
PH12013501592A1 (en) Novel use of a dense and erect panicle 1 gene in improving nitrogen utilization efficiency
NZ601950A (en) Method for producing pyripyropene
MX393132B (es) Polinucleotidos y polipeptidos aislados, y metodos para utilizarlos para aumentar la eficacia en el uso de nitrogeno, rendimiento, tasa de crecimiento, vigor, biomasa, contenido de aceite y/o tolerancia al estres abiotico.
AR114287A2 (es) Constructo de un gen quimérico aislado; polipéptido codificado por dicho constructo; y método para conferir a una planta tolerancia a sequía, alta salinidad y congelamiento, excluida la planta obtenida por dicho método
MY152746A (en) Altering enzyme balance through fermentation conditions
MX2018005935A (es) Plantas modificadas que contienen una combinacion de genes de apirasa y metodo para obtener plantas modificadas con combinacion de genes de apirasa.
AR047590A1 (es) Alteracion de la estructura de la raiz durante el desarrollo de la planta
AR106087A1 (es) Promotor y 3’ utr de plantas para la expresión del transgén
MY157117A (en) Polynucleotides and methods for improving plants
IN2012DN01315A (es)
AR092217A1 (es) Cepas de levadura modificadas para producir etanol a partir de acetato
NZ629391A (en) Plants having improved growth properties
IN2012DN01359A (es)
AR069689A2 (es) Polinucleotido recombinante, construccion de acido nucleico, polipeptido recombinante que tiene actividad de glifosato-n-acetiltransferasa
AR069555A1 (es) Polinucleotidos que codifican polipeptidos vegetales, y metodos para usarlos para incrementar tolerancia a estres abiotico y/o biomasa y/o rendimiento en plantas que los expresan
AR085899A1 (es) Plantas que tienen mejores rasgos relacionados con el rendimiento y un metodo para producirlas