ES2748130T3 - Dispositivos y métodos para la producción de un biomaterial - Google Patents

Dispositivos y métodos para la producción de un biomaterial Download PDF

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Publication number
ES2748130T3
ES2748130T3 ES04753608T ES04753608T ES2748130T3 ES 2748130 T3 ES2748130 T3 ES 2748130T3 ES 04753608 T ES04753608 T ES 04753608T ES 04753608 T ES04753608 T ES 04753608T ES 2748130 T3 ES2748130 T3 ES 2748130T3
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ES
Spain
Prior art keywords
fluid
tank
interest
solution
plasmid
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
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ES04753608T
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English (en)
Spanish (es)
Inventor
Henry Hebel
Sriram Ramakrishnan
Hugo Gonzalez
Jeff Darnell
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VGXI Inc
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VGXI Inc
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=33511626&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ES2748130(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by VGXI Inc filed Critical VGXI Inc
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Publication of ES2748130T3 publication Critical patent/ES2748130T3/es
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01FMIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00Other mixers; Mixing plants; Combinations of mixers
    • B01F33/40Mixers using gas or liquid agitation, e.g. with air supply tubes
    • B01F33/406Mixers using gas or liquid agitation, e.g. with air supply tubes in receptacles with gas supply only at the bottom
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/06Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/10Separation or concentration of fermentation products
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06Lysis of microorganisms
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06Lysis of microorganisms
    • C12N1/066Lysis of microorganisms by physical processes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B03SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03DFLOTATION; DIFFERENTIAL SEDIMENTATION
    • B03D2203/00Specified materials treated by the flotation agents; Specified applications
    • B03D2203/003Biotechnological applications, e.g. separation or purification of enzymes, hormones, vitamins, viruses

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Mycology (AREA)
  • Plant Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Sustainable Development (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
ES04753608T 2003-05-30 2004-05-27 Dispositivos y métodos para la producción de un biomaterial Expired - Lifetime ES2748130T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US47474903P 2003-05-30 2003-05-30
PCT/US2004/016806 WO2004108260A2 (fr) 2003-05-30 2004-05-27 Dispositifs et procedes de production de biomateriaux

Publications (1)

Publication Number Publication Date
ES2748130T3 true ES2748130T3 (es) 2020-03-13

Family

ID=33511626

Family Applications (1)

Application Number Title Priority Date Filing Date
ES04753608T Expired - Lifetime ES2748130T3 (es) 2003-05-30 2004-05-27 Dispositivos y métodos para la producción de un biomaterial

Country Status (7)

Country Link
US (1) US7238522B2 (fr)
EP (2) EP1628749B1 (fr)
CA (1) CA2567324C (fr)
DK (2) DK1628749T3 (fr)
ES (1) ES2748130T3 (fr)
TW (1) TWI303275B (fr)
WO (1) WO2004108260A2 (fr)

Families Citing this family (65)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7248938B2 (en) 2003-12-31 2007-07-24 Vigilistics, Inc. Method and system for monitoring batch product manufacturing
DE102004052254A1 (de) * 2004-05-03 2005-12-01 Plasmid Factory Gmbh & Co. Kg Verfahren zum Aufschluss von Zellen und zur Abtrennung von Zellbestandteilen
US7846720B2 (en) * 2005-01-26 2010-12-07 Vgx Pharmaceuticals, Inc. Optimized high yield synthetic plasmids
AT501380B1 (de) * 2005-02-07 2007-11-15 Vitali Dipl Ing Dshemelinski Verfahren zur reinigung der dna/rna von proteinen und zellulären reststoffen im zellenlysat
CA2666501C (fr) 2006-10-17 2017-03-21 Vgx Pharmaceuticals, Inc. Dispositifs d'electroporation et leurs procedes d'utilisation pour l'electroporation de cellules de mammiferes
JP5793726B2 (ja) * 2007-05-23 2015-10-14 ブイジーエックスアイ,インコーポレイティド 高濃度の生物学的に活性な分子を含む組成物とその製造方法
US20080299621A1 (en) * 2007-05-31 2008-12-04 Ge Healthcare Uk Limited Miniprep system for simple and rapid plasmid dna extraction
CN101679509A (zh) 2007-06-01 2010-03-24 弗·哈夫曼-拉罗切有限公司 免疫球蛋白纯化
US7989615B2 (en) * 2007-07-13 2011-08-02 The Penn State Research Foundation Separation of different isoforms of plasmid DNA using ultrafiltration
CA2705461A1 (fr) 2007-11-12 2009-06-11 The Trustees Of The University Of Pennsylvania Vaccins contre plusiseurs sous-types de virus de l'influenza
KR20100100868A (ko) * 2007-11-14 2010-09-15 브이지엑스 파마수티컬즈, 엘엘씨 전기천공에 의해 전달되는 dna 백신에 의해 유도되는 항체의 생산 방법
KR101686942B1 (ko) 2008-01-11 2016-12-28 이노비오 파마수티컬즈, 인크. 뎅기 바이러스 다중 서브타입에 대항하는 신규한 백신
EP2088196A1 (fr) * 2008-02-08 2009-08-12 Boehringer Ingelheim RCV GmbH & Co KG Procédés et dispositifs pour la production de biomolécules
CA2725383C (fr) 2008-05-28 2018-09-25 Ruxandra Draghia-Akli Vaccin antivariolique a adn et antigenes presents a l'interieur capables de susciter une reponse immunitaire
EP2358733B1 (fr) 2008-11-17 2015-07-08 Inovio Pharmaceuticals, Inc. Antigènes qui induisent une réponse immunitaire contre les flavivirus et procédés d' utilisation de ceux-ci
KR102138780B1 (ko) 2009-11-02 2020-07-29 더 트러스티스 오브 더 유니버시티 오브 펜실바니아 구제역 바이러스(fmdv) 공통 단백질, 이를 위한 코딩 서열 및 이로부터 만들어진 백신
MX2012005091A (es) * 2009-11-02 2012-06-19 Mannkind Corp Reactor para producir particulas farmaceuticas en un procedimiento de precipitacion.
US8298820B2 (en) 2010-01-26 2012-10-30 The Trustees Of The University Of Pennsylvania Influenza nucleic acid molecules and vaccines made therefrom
EP2539354B1 (fr) 2010-02-08 2017-04-05 The Trustees Of The University Of Pennsylvania Molécules d'acide nucléique codant pour rantes, compositions les comprenant et procédés les utilisant
MX355501B (es) 2010-09-27 2018-04-20 Inovio Pharmaceuticals Inc Star Estructuras de consenso de antigenos y vacunas elaboradas a partir de estas y metodos para utilizarlas en el tratamiento de la malaria.
JP6077450B2 (ja) 2010-11-12 2017-02-08 ザ トラスティーズ オブ ザ ユニバーシティ オブ ペンシルバニア コンセンサス前立腺抗原、それをコードする核酸分子、ならびにそれを含むワクチンおよび用途
EA033467B1 (ru) 2011-01-31 2019-10-31 Univ Pennsylvania Молекула нуклеиновой кислоты для индукции иммунного ответа против герпесвируса и ее применение
EA032364B1 (ru) 2011-02-11 2019-05-31 Дзе Трастиз Оф Дзе Юниверсити Оф Пенсильвания Молекула нуклеиновой кислоты, кодирующая коровый белок вируса гепатита в, и вакцина, содержащая указанную молекулу
US9238679B2 (en) 2011-02-11 2016-01-19 The Trustees Of The University Of Pennslyvania Nucleic acid molecule encoding hepatitis B virus core protein and surface antigen protein and vaccine comprising the same
KR102314421B1 (ko) 2011-07-11 2021-10-19 이노비오 파마수티컬즈, 인크. 교차-보호성 아레나바이러스 백신 및 이의 사용 방법
JP6158819B2 (ja) * 2011-11-10 2017-07-05 エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft 分取カラムクロマトグラフィシステム
JP6333730B2 (ja) 2011-12-12 2018-05-30 ザ トラスティーズ オブ ザ ユニバーシティ オブ ペンシルバニア 改善されたil−12遺伝子構築物を含む組成物、及びそれを用いたワクチン、免疫治療剤及び方法
JP6407028B2 (ja) 2011-12-12 2018-10-17 ザ トラスティーズ オブ ザ ユニバーシティ オブ ペンシルバニア MRSAのPBP2a及びその断片を含むタンパク質、それをコードする核酸、並びにMRSA感染を予防する及び治療するための組成物及びそれらの使用
HK1210479A1 (en) 2012-04-10 2016-04-22 The Trustees Of The University Of Pennsylvania Human respiratory syncytial virus consensus antigens, nucleic acid constructs and vaccines made therefrom, and methods of using same
CA3194125A1 (fr) 2012-04-12 2013-10-17 The Trustees Of The University Of Pennsylvania Antigenes consensus de filovirus, constructions d'acides nucleiques et vaccins a base de ceux-ci, et leurs methodes d'utilisation
CN102854052B (zh) * 2012-08-08 2015-01-07 长春迪瑞医疗科技股份有限公司 一种气泡混匀方法及其控制系统
CN104812401B (zh) 2012-12-13 2017-10-13 宾夕法尼亚大学理事会 Wt1疫苗
WO2015089492A2 (fr) 2013-12-13 2015-06-18 The Trustees Of The University Of Pennsylvania Constructions d'anticorps d'adn et procédé d'utilisation
US9556427B2 (en) * 2013-02-27 2017-01-31 Syngenta Participations Ag Methods and compositions for preparation of nucleic acids
KR20150130284A (ko) 2013-03-15 2015-11-23 더 트러스티스 오브 더 유니버시티 오브 펜실바니아 암 백신 및 이를 이용한 치료 방법
CN105338998B (zh) 2013-03-15 2021-12-14 宾夕法尼亚大学理事会 口蹄疫病毒(fmdv)共有蛋白、其编码序列以及由其制造的疫苗
ES2626963T3 (es) * 2014-07-25 2017-07-26 Vito Nv (Vlaamse Instelling Voor Technologisch Onderzoek Nv) Método y aparato para la disrupción de células de biomasa
MX2017007187A (es) 2014-12-01 2018-01-30 Univ Pennsylvania Construcciones de anticuerpos de adn y metodo para utilizarlas.
CN106884011B (zh) * 2015-12-16 2020-11-17 固安鼎泰海规生物科技有限公司 一种规模化质粒纯化的联合液相层析分离方法
WO2017136758A1 (fr) 2016-02-05 2017-08-10 Inovio Pharmaceuticals, Inc. Vaccins anticancéreux et méthodes de traitement les utilisant
US10774322B2 (en) 2016-12-29 2020-09-15 Shoreline Biome, Llc Combined lysis protocol for comprehensive cell lysis
US11149246B2 (en) 2016-12-29 2021-10-19 Shoreline Biome, Llc Methods for cell lysis and preparation of high molecular weight DNA from modified cells
KR102668476B1 (ko) 2017-12-13 2024-05-23 이노비오 파마수티컬즈, 인크. Boris를 표적으로 하는 암 백신 및 이의 용도
WO2019118764A1 (fr) 2017-12-13 2019-06-20 Inovio Pharmaceuticals, Inc. Vaccins anticancer ciblant muc16 et leurs utilisations
WO2019118760A1 (fr) 2017-12-13 2019-06-20 Inovio Pharmaceuticals, Inc. Vaccins anti-cancéreux ciblant la mésothéline et leurs utilisations
CA3083532C (fr) 2017-12-13 2023-09-05 Inovio Pharmaceuticals, Inc. Vaccins anticancereux ciblant l'antigene exprime de facon preferentielle dans le melanome (prame) et ses utilisations
CN108298626A (zh) * 2018-01-15 2018-07-20 天津科创复兴科技咨询有限公司 一种废水曝气净化装置
MX2020012910A (es) * 2018-05-31 2021-05-27 Vgxi Inc Aparato de espiral de lisis y usos del mismo para el aislamiento y purificacion de polinucleotidos.
EP3594338A1 (fr) * 2018-07-12 2020-01-15 Kaneka Eurogentec SA Procédé et appareil pour la purification de séquences d'acides nucléiques extra-chromosomiques
US12239970B2 (en) * 2018-11-27 2025-03-04 Ddp Specialty Electronic Materials Us, Llc Method for reducing dilution effects in fluid treatment vessels
CN112940061B (zh) 2019-12-10 2024-05-03 财团法人工业技术研究院 核酸萃取装置
CN111073885A (zh) * 2019-12-31 2020-04-28 江苏耀海生物制药有限公司 一种应用于双链dna片段的纯化方法
CN111662807A (zh) * 2020-07-14 2020-09-15 漳州卫生职业学院 染色体自动收获装置及其自动收获方法
CN111979109A (zh) * 2020-09-01 2020-11-24 深圳普瑞金生物药业有限公司 质粒载体连续裂解装置
CN114164113A (zh) * 2020-09-10 2022-03-11 西比曼生物科技(香港)有限公司 一种封闭式的菌体裂解工艺
CN114181931B (zh) * 2020-10-19 2023-12-26 艾棣维欣(苏州)生物制品有限公司 一种从细菌中提取质粒dna的方法
CN114381355B (zh) * 2020-10-19 2024-07-30 艾棣维欣(苏州)生物制品有限公司 用于提取细菌中质粒dna的气泡发生装置
CN115404232A (zh) * 2021-05-10 2022-11-29 艾棣维欣(苏州)生物制品有限公司 用于提取细菌中质粒dna的方法
WO2022271955A1 (fr) 2021-06-23 2022-12-29 Musc Foundation For Research Development Nouvelles nanoparticules de sharn ciblées pour la thérapie du cancer
CN114561273A (zh) * 2022-04-02 2022-05-31 北京沃森创新生物技术有限公司 一种制备质粒的连续碱裂解系统以及质粒的制备方法
CN114933960A (zh) * 2022-07-03 2022-08-23 苏州谱博分析技术有限公司 一种连续流细菌破碎与澄清装置
AU2023365103A1 (en) * 2022-10-19 2025-04-24 Abec, Inc. Cell lysis and plasmid isolation system
JP2026510008A (ja) * 2023-03-13 2026-03-27 ホンブレヒティコン システムズ エンジニアリング アクチェンゲゼルシャフト 混合物を提供するための混合装置及び方法
CN117431149B (zh) * 2023-12-22 2024-03-08 北京艺妙神州医药科技有限公司 一种菌体的洗涤方法
WO2026015658A1 (fr) * 2024-07-09 2026-01-15 The General Hospital Corporation Fluidique à disque intégré pour traitement d'échantillon à haut débit

Family Cites Families (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CH453316A (de) * 1964-11-10 1968-06-14 Inventa Ag Verfahren zur Herstellung von Hydroxylamin durch katalytische Hydrierung von Stickoxyd und Vorrichtung zur Durchführung dieses Verfahrens
US4533123A (en) 1984-05-07 1985-08-06 Betz Laboratories, Inc. Liquid mixing device
US5438128A (en) 1992-02-07 1995-08-01 Millipore Corporation Method for rapid purifiction of nucleic acids using layered ion-exchange membranes
DE69324716T2 (de) 1992-02-13 1999-09-09 Becton Dickinson And Co. Celithydrat und Reinigung von DNS
US5482836A (en) 1993-01-14 1996-01-09 The Regents Of The University Of California DNA purification by triplex-affinity capture and affinity capture electrophoresis
US5591841A (en) 1993-01-14 1997-01-07 Ji; Huamin Rapid purification of circular DNA by triplex-mediated affinity capture
CA2170604C (fr) 1993-08-30 2007-03-13 Vikas V. Padhye Compositions et methodes pour la purification de l'acide nucleique
US5438127A (en) 1993-09-27 1995-08-01 Becton Dickinson And Company DNA purification by solid phase extraction using a PCl3 modified glass fiber membrane
US5561064A (en) 1994-02-01 1996-10-01 Vical Incorporated Production of pharmaceutical-grade plasmid DNA
US5990301A (en) 1994-02-07 1999-11-23 Qiagen Gmbh Process for the separation and purification of nucleic acids from biological sources
GB9411572D0 (en) 1994-06-09 1994-08-03 Amersham Int Plc Magnetic bead precipitation method
US6197553B1 (en) 1994-07-15 2001-03-06 Merck & Co., Inc. Method for large scale plasmid purification
US5625053A (en) 1994-08-26 1997-04-29 Board Of Regents For Northern Illinois Univ. Method of isolating purified plasmid DNA using a nonionic detergent, solution
US5705628A (en) 1994-09-20 1998-01-06 Whitehead Institute For Biomedical Research DNA purification and isolation using magnetic particles
US5837529A (en) 1994-10-17 1998-11-17 Genzyme Corporation Method for lysing cells
US5981735A (en) 1996-02-12 1999-11-09 Cobra Therapeutics Limited Method of plasmid DNA production and purification
FR2746412B1 (fr) 1996-03-21 1998-06-12 Rhone Poulenc Rorer Sa Purification d'adn plasmidique de qualite pharmaceutique
US7026468B2 (en) 1996-07-19 2006-04-11 Valentis, Inc. Process and equipment for plasmid purification
US7807822B2 (en) 1996-08-01 2010-10-05 Robert Bridenbaugh Methods for purifying nucleic acids
US6011148A (en) 1996-08-01 2000-01-04 Megabios Corporation Methods for purifying nucleic acids
US5707812A (en) 1996-08-06 1998-01-13 Vical Incorporated Purification of plasmid DNA during column chromatography
US5843731A (en) 1996-09-05 1998-12-01 Asahi Kogaku Kogyo Kabushiki Kaisha Method for purifying plasmid DNA on calcium phosphate compound
EP0853123A1 (fr) 1997-01-10 1998-07-15 Roche Diagnostics GmbH Purification d'ADN à l'aide de 'cross-flow-filtration'
US5986085A (en) 1997-04-25 1999-11-16 Transgenomic, Inc. Matched ion polynucleotide chromatography (MIPC) process for separation of polynucleotide fragments
SE9703532D0 (sv) 1997-09-30 1997-09-30 Pharmacia Biotech Ab A process for the purification of plasmid DNA
GB2338236B (en) 1998-06-13 2003-04-09 Aea Technology Plc Microbiological cell processing
DE19900681B4 (de) 1999-01-04 2007-04-12 Sartorius Ag Verwendung von Anionaustauschermembranen zur Entfernung von Endotoxinen aus Nukleinsäure-haltigen Lösungen
KR20020009566A (ko) 1999-03-11 2002-02-01 추후제출 세포 용해물의 혼합용 용기
GB9905646D0 (en) 1999-03-11 1999-05-05 Cobra Therapeutics Ltd A vessel for mixing a cell lysate
DE60125035T2 (de) 2000-06-02 2007-06-21 Pall Corp. Behandlung von plasmid-haltigen lösungen
US7314746B2 (en) 2002-09-13 2008-01-01 Valentis, Inc. Apparatus and method for preparative scale purification of nucleic acids

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EP1628749A2 (fr) 2006-03-01
WO2004108260A2 (fr) 2004-12-16
US20050014245A1 (en) 2005-01-20
EP2364769B1 (fr) 2016-01-13
EP2364769A1 (fr) 2011-09-14
DK1628749T3 (da) 2019-09-16
US7238522B2 (en) 2007-07-03
WO2004108260A3 (fr) 2005-05-12
CA2567324A1 (fr) 2004-12-16
CA2567324C (fr) 2012-01-03
EP1628749B1 (fr) 2019-07-31
TW200504207A (en) 2005-02-01
TWI303275B (en) 2008-11-21
DK2364769T3 (en) 2016-04-11

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