ES2861353T3 - Amplificación específica de alelo utilizando una composición de oligonucleótidos cebadores no específicos de alelo y bloqueantes específicos de alelo solapantes - Google Patents

Amplificación específica de alelo utilizando una composición de oligonucleótidos cebadores no específicos de alelo y bloqueantes específicos de alelo solapantes Download PDF

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Publication number: ES2861353T3
Authority: ES; Spain
Prior art keywords: subsequence; sequence; target; oligonucleotide; dna
Prior art date: 2014-05-19
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Active

Application number

ES15796383T

Other languages

English (en)

Spanish (es)

Inventor

David Yu Zhang

Ruojia Wu

Juexiao Wang

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

William Marsh Rice University

Original Assignee

William Marsh Rice University

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2014-05-19

Filing date

2015-05-19

Publication date

2021-10-06

2015-05-19 Application filed by William Marsh Rice University filed Critical William Marsh Rice University

2021-10-06 Application granted granted Critical

2021-10-06 Publication of ES2861353T3 publication Critical patent/ES2861353T3/es

Status Active legal-status Critical Current

2035-05-19 Anticipated expiration legal-status Critical

Links

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Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
- C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B30/00—Methods of screening libraries
- C40B30/04—Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2527/00—Reactions demanding special reaction conditions
- C12Q2527/107—Temperature of melting, i.e. Tm
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
- C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
- C12Q2537/163—Reactions characterised by the reaction format or use of a specific feature the purpose or use of blocking probe

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Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Life Sciences & Earth Sciences (AREA)
Health & Medical Sciences (AREA)
Engineering & Computer Science (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Chemical Kinetics & Catalysis (AREA)
Molecular Biology (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
Biotechnology (AREA)
Genetics & Genomics (AREA)
Analytical Chemistry (AREA)
Immunology (AREA)
General Engineering & Computer Science (AREA)
Bioinformatics & Cheminformatics (AREA)
Microbiology (AREA)
Physics & Mathematics (AREA)
Biophysics (AREA)
General Chemical & Material Sciences (AREA)
Medicinal Chemistry (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Enzymes And Modification Thereof (AREA)

ES15796383T 2014-05-19 2015-05-19 Amplificación específica de alelo utilizando una composición de oligonucleótidos cebadores no específicos de alelo y bloqueantes específicos de alelo solapantes Active ES2861353T3 (es)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
US201462000114P	2014-05-19	2014-05-19
PCT/US2015/031476 WO2015179339A1 (en)	2014-05-19	2015-05-19	Allele-specific amplification using a composition of overlapping non-allele-specific primer and allele-specific blocker oligonucleotides

Publications (1)

Publication Number	Publication Date
ES2861353T3 true ES2861353T3 (es)	2021-10-06

Family

ID=54554607

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
ES15796383T Active ES2861353T3 (es)	2014-05-19	2015-05-19	Amplificación específica de alelo utilizando una composición de oligonucleótidos cebadores no específicos de alelo y bloqueantes específicos de alelo solapantes

Country Status (13)

Country	Link
US (2)	US20170067090A1 (2)
EP (3)	EP3146080B1 (2)
JP (4)	JP2017515484A (2)
KR (1)	KR102343605B1 (2)
CN (2)	CN106661627B (2)
AU (2)	AU2015264357B2 (2)
BR (1)	BR112016027063A2 (2)
CA (1)	CA2985631A1 (2)
DK (1)	DK3146080T3 (2)
ES (1)	ES2861353T3 (2)
PH (1)	PH12016502542B1 (2)
RU (1)	RU2708992C2 (2)
WO (1)	WO2015179339A1 (2)

Families Citing this family (56)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US8835358B2 (en)	2009-12-15	2014-09-16	Cellular Research, Inc.	Digital counting of individual molecules by stochastic attachment of diverse labels
CA2865575C (en)	2012-02-27	2024-01-16	Cellular Research, Inc.	Compositions and kits for molecular counting
KR102758333B1 (ko)	2013-08-28	2025-01-23	벡톤 디킨슨 앤드 컴퍼니	대량의 동시 단일 세포 분석
WO2015077717A1 (en)	2013-11-25	2015-05-28	The Broad Institute Inc.	Compositions and methods for diagnosing, evaluating and treating cancer by means of the dna methylation status
WO2015085147A1 (en)	2013-12-05	2015-06-11	The Broad Institute Inc.	Polymorphic gene typing and somatic change detection using sequencing data
KR20230076867A (ko)	2013-12-20	2023-05-31	더 브로드 인스티튜트, 인코퍼레이티드	신생항원 백신과의 병용 요법
EP3234130B1 (en)	2014-12-19	2020-11-25	The Broad Institute, Inc.	Methods for profiling the t-cell- receptor repertoire
US10975442B2 (en)	2014-12-19	2021-04-13	Massachusetts Institute Of Technology	Molecular biomarkers for cancer immunotherapy
WO2016138496A1 (en)	2015-02-27	2016-09-01	Cellular Research, Inc.	Spatially addressable molecular barcoding
WO2016160844A2 (en)	2015-03-30	2016-10-06	Cellular Research, Inc.	Methods and compositions for combinatorial barcoding
EP4582556A3 (en)	2015-04-23	2025-10-08	Becton, Dickinson and Company	Methods and compositions for whole transcriptome amplification
JP6940484B2 (ja)	2015-09-11	2021-09-29	セルラーリサーチ，インコーポレイテッド	ライブラリー正規化のための方法および組成物
CN108603193B (zh) *	2015-12-30	2022-07-22	生物辐射实验室股份有限公司	分裂循环和tape扩增
WO2017193025A1 (en)	2016-05-06	2017-11-09	William Marsh Rice University	Stoichiometric nucleic acid purification using randomer capture probe libraries
US10301677B2 (en)	2016-05-25	2019-05-28	Cellular Research, Inc.	Normalization of nucleic acid libraries
US10640763B2 (en)	2016-05-31	2020-05-05	Cellular Research, Inc.	Molecular indexing of internal sequences
US10202641B2 (en)	2016-05-31	2019-02-12	Cellular Research, Inc.	Error correction in amplification of samples
CN109791157B (zh)	2016-09-26	2022-06-07	贝克顿迪金森公司	使用具有条形码化的寡核苷酸序列的试剂测量蛋白质表达
US11549149B2 (en) *	2017-01-24	2023-01-10	The Broad Institute, Inc.	Compositions and methods for detecting a mutant variant of a polynucleotide
CN110382708A (zh)	2017-02-01	2019-10-25	赛卢拉研究公司	使用阻断性寡核苷酸进行选择性扩增
CN110719959B (zh)	2017-06-05	2021-08-06	贝克顿迪金森公司	针对单细胞的样品索引
JP2021500077A (ja) *	2017-10-18	2021-01-07	デイゼロダイアグノスティックス，インコーポレイテッド	Ｄｎａ増幅の標的化抑制による混合ｄｎａ試料中のｄｎａ集団の選択的富化
EP3759118A4 (en) *	2018-02-20	2022-03-23	William Marsh Rice University	Systems and methods for allele enrichment using multiplexed blocker displacement amplification
CN108220444A (zh) *	2018-02-28	2018-06-29	无锡禾盛医疗器械有限公司	一种kras基因突变检测的引物探针组合及其应用
JP7358388B2 (ja)	2018-05-03	2023-10-10	ベクトン・ディキンソン・アンド・カンパニー	反対側の転写物末端における分子バーコーディング
CN112272710A (zh)	2018-05-03	2021-01-26	贝克顿迪金森公司	高通量多组学样品分析
ES2992135T3 (es)	2018-10-01	2024-12-09	Becton Dickinson Co	Determinar secuencias de transcripción 5
US11932849B2 (en)	2018-11-08	2024-03-19	Becton, Dickinson And Company	Whole transcriptome analysis of single cells using random priming
US11492660B2 (en)	2018-12-13	2022-11-08	Becton, Dickinson And Company	Selective extension in single cell whole transcriptome analysis
US20200224260A1 (en) *	2019-01-15	2020-07-16	Tangen Bioscience Inc.	Method for suppressing non-specific amplification products in nucleic acid amplification technologies
ES2945227T3 (es)	2019-01-23	2023-06-29	Becton Dickinson Co	Oligonucleótidos asociados con anticuerpos
WO2020167920A1 (en)	2019-02-14	2020-08-20	Cellular Research, Inc.	Hybrid targeted and whole transcriptome amplification
US20220348606A1 (en) *	2019-06-26	2022-11-03	Nanjing GenScript Biotech Co., Ltd.	Oligonucleotide containing blocker
CN120099137A (zh)	2019-07-22	2025-06-06	贝克顿迪金森公司	单细胞染色质免疫沉淀测序测定
JP7522189B2 (ja)	2019-11-08	2024-07-24	ベクトン・ディキンソン・アンド・カンパニー	免疫レパートリーシーケンシングのための完全長ｖ（ｄ）ｊ情報を得るためのランダムプライミングの使用
US20240279735A1 (en) *	2019-12-20	2024-08-22	Korea University Research And Business Foundation	Primers for detecting trace amount of rare single- nucleotide variant and method for specifically and sensitively detecting trace amount of rare single-nucleotide variant by using same
US11649497B2 (en)	2020-01-13	2023-05-16	Becton, Dickinson And Company	Methods and compositions for quantitation of proteins and RNA
WO2021146219A1 (en)	2020-01-13	2021-07-22	Becton, Dickinson And Company	Cell capture using du-containing oligonucleotides
WO2021155057A1 (en)	2020-01-29	2021-08-05	Becton, Dickinson And Company	Barcoded wells for spatial mapping of single cells through sequencing
WO2021173719A1 (en)	2020-02-25	2021-09-02	Becton, Dickinson And Company	Bi-specific probes to enable the use of single-cell samples as single color compensation control
US20230220456A1 (en) *	2020-05-01	2023-07-13	William Marsh Rice University	Quantitative blocker displacement amplification (qbda) sequencing for calibration-free and multiplexed variant allele frequency quantitation
US11661625B2 (en)	2020-05-14	2023-05-30	Becton, Dickinson And Company	Primers for immune repertoire profiling
US12157913B2 (en)	2020-06-02	2024-12-03	Becton, Dickinson And Company	Oligonucleotides and beads for 5 prime gene expression assay
CN116075520A (zh) *	2020-06-26	2023-05-05	威廉马歇莱思大学	扩增子全面富集
CN111647650B (zh) *	2020-07-06	2023-06-27	河南赛诺特生物技术有限公司	检测人B-raf基因V600E突变的引物、引物探针组合物及试剂盒
US11932901B2 (en)	2020-07-13	2024-03-19	Becton, Dickinson And Company	Target enrichment using nucleic acid probes for scRNAseq
US12391940B2 (en)	2020-07-31	2025-08-19	Becton, Dickinson And Company	Single cell assay for transposase-accessible chromatin
WO2022094403A1 (en) *	2020-11-02	2022-05-05	Nuprobe Usa, Inc.	Quantitative multiplex amplicon sequencing system
EP4247967A1 (en)	2020-11-20	2023-09-27	Becton, Dickinson and Company	Profiling of highly expressed and lowly expressed proteins
CN112522369A (zh) *	2020-12-08	2021-03-19	合肥欧创基因生物科技有限公司	一种ARMS-TaqMan Blocker体系的Blocker双链设计方法
CN116685850A (zh)	2020-12-15	2023-09-01	贝克顿迪金森公司	单细胞分泌组分析
WO2022146773A1 (en)	2020-12-29	2022-07-07	Nuprobe Usa, Inc.	Methods and compositions for sequencing and fusion detection using ligation tail adapters (lta)
WO2022155397A1 (en)	2021-01-15	2022-07-21	Nuprobe Usa, Inc.	Cycle multiplexing for highly multiplexed quantitative pcr
WO2022197688A1 (en)	2021-03-16	2022-09-22	Nuprobe Usa, Inc.	High sensitivity detection of microsatellite loci by blocker displacement amplification with multiple blockers and its use in microsatellite instability detection
WO2023077121A1 (en) *	2021-11-01	2023-05-04	Nuprobe Usa, Inc.	Rna quantitative amplicon sequencing for gene expression quantitation
CN119662835B (zh) *	2025-02-20	2025-05-27	宁波熙宁检测技术有限公司	一种用于检测ezh2基因突变的核酸组合物、试剂盒及检测方法

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US6312892B1 (en) *	1996-07-19	2001-11-06	Cornell Research Foundation, Inc.	High fidelity detection of nucleic acid differences by ligase detection reaction
ATE541055T1 (de) *	2004-10-18	2012-01-15	Univ Brandeis	Reagenzien und verfahren zur verbesserung der reproduzierbarkeit und zur reduzierung des mispriming bei der pcr-amplifikation
WO2007106534A2 (en) *	2006-03-14	2007-09-20	Harbor-Ucla Research And Education Institute	Selective amplification of minority mutations using primer blocking high-affinity oligonucleotides
US8071338B2 (en) *	2007-08-08	2011-12-06	Roche Molecular Systems, Inc.	Suppression of amplification using an oligonucleotide and a polymerase significantly lacking 5′-3′ nuclease activity
JP5535921B2 (ja) *	2007-10-04	2014-07-02	コモンウェルスサイエンティフィックアンドインダストリアルリサーチオーガナイゼーション	核酸増幅
CN105200097A (zh) *	2008-10-20	2015-12-30	霍夫曼-拉罗奇有限公司	改进的等位基因特异性扩增
US8206929B2 (en) *	2009-01-07	2012-06-26	Roche Molecular Systems, Inc.	Nucleic acid amplification with allele-specific suppression of sequence variants
EP2411543B1 (en) *	2009-03-27	2017-04-19	Life Technologies Corporation	Methods, compositions, and kits for detecting allelic variants
KR101312241B1 (ko) *	2010-04-27	2013-09-27	사회복지법인 삼성생명공익재단	증폭억제시발체를 이용하는 유전자 돌연변이 검출 방법
US20130059294A1 (en) *	2010-05-16	2013-03-07	Xiangdong Ren	Identification of polymorphic hepatitis b viruses and kras oncogene mutations and clinical use
GB2487632B (en) *	2011-01-14	2014-03-12	Genefirst Ltd	Methods,compositions,and kits for determining the presence/absence of a variant nucleic acid sequence
KR20210056458A (ko) *	2011-05-04	2021-05-18	바이오셉트 인코포레이티드	핵산 서열 변이체를 검출하는 방법
EP2798078B1 (en) *	2011-12-30	2018-02-14	Polowang Biotech Inc.	Method and primer set for detecting mutation
US9879316B2 (en) *	2012-04-25	2018-01-30	Tho Huu Ho	Method for competitive allele-specific cDNA synthesis and differential amplification of the cDNA products
CN103215361A (zh) *	2013-04-18	2013-07-24	深圳联合医学科技有限公司	等位基因变体检测方法、试剂盒及组合物
EP2992110B1 (en) *	2013-04-29	2017-10-25	QIAGEN Marseille S.A.S.	A method for dna amplification with a blocking oligonucleotide

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WO2015179339A1 (en)	2015-11-26
RU2016149307A3 (2)	2018-12-10
RU2708992C2 (ru)	2019-12-12
PH12016502542B1 (en)	2024-01-17
CN114032234A (zh)	2022-02-11
AU2021266291A1 (en)	2021-12-09
CN106661627A (zh)	2017-05-10
BR112016027063A2 (pt)	2017-10-31
EP3146080A1 (en)	2017-03-29
AU2021266291C1 (en)	2024-12-12
EP3901278B1 (en)	2024-10-23
CN106661627B (zh)	2021-11-23
CA2985631A1 (en)	2015-11-26
JP2022166131A (ja)	2022-11-01
JP7126718B2 (ja)	2022-08-29
EP4512908A2 (en)	2025-02-26
EP4512908A3 (en)	2025-03-26
AU2015264357A1 (en)	2017-01-05
JP2021019607A (ja)	2021-02-18
JP2024167406A (ja)	2024-12-03
DK3146080T3 (en)	2021-04-12
US20220090168A1 (en)	2022-03-24
PH12016502542A1 (en)	2017-04-10
EP3146080A4 (en)	2017-11-01
AU2015264357B2 (en)	2021-08-12
JP2017515484A (ja)	2017-06-15
KR102343605B1 (ko)	2021-12-27
EP3901278A1 (en)	2021-10-27
KR20170003695A (ko)	2017-01-09
RU2016149307A (ru)	2018-06-20
EP3146080B1 (en)	2021-01-06
US20170067090A1 (en)	2017-03-09

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Murray et al.	2014	Seven novel probe systems for real-time PCR provide absolute single-base discrimination, higher signaling, and generic components
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ES2565331T3 (es)	2016-04-04	Análisis de dosificación génica
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