ES303970A1 - PROCEDURE FOR THE PRODUCTION OF A POLYPEPTURE. - Google Patents

Abstract

Procedure for the production of a polypeptide, especially tetracosapeptide L-seryl-L-tyrosyl-L-seryl-L-norleucyl-L-glutamyl-L-histidyl-L-phenylalanyl-L-arginyl-L-tryptophanyl-glycyl-L -lisyl-L-prolyl-L-valyl-glycyl-L-lysyl-L-lysyl-L-arginyl-L-arginyl-L-prolyl-L-valyl-L-lysyl-L-valyl-L-tyrosyl-L -proline and, characterized in that the heptapeptide derivative N-carbobenzoxy-L-valyl-glycyl ε -N-formyl-L-lysyl- ε -N-formyl-L-lysyl-N-tosyl-L is treated -arginyl-N-tosyl-L-arginyl-L-proline with sodium in dry liquid ammonia, the L-valyl-glycyl- ε -N-formyl-L-lysyl- ε -N-formyl-L- is condensed Lysyl-L-arginyl-L-arginyl-proline resulting with NRL-seryl-L-tyrosyl-L-seryl-L-norleucyl- γ -O-butyl (tert.) - L-glutamyl-L-histidyl-L- phenylalanyl-L-arginyl-L-tryptophanyl-glycyl ε -N-formyl-L-lysyl-L-proline, wherein R means a suitable protecting group, preferably a carbobenzoxy, acetyl, formyl, carbobutoxy radical (tert. ), p-nitrocarbobenzoxy or triphenyl tyl, the NRL-seryl-L-tyrosyl-L-seryl-L-norleucyl- γ -O-butyl (tert.) - L-glutamyl-L-histidyl-L-phenyl-alanyl-L is then reacted -arginyl-L-tryptophanyl-glycyl ε -N-formyl-L-lysyl-L-prolyl-L-valyl-glycyl- ε -N-formyl-L-lysyl- ε -L-lysyl-L- resulting arginyl-L-arginyl-L-proline, in which R has the meaning indicated above, with ester L-valil- ε -N-formyl-L-lysyl-L-valyl-L-tyrosyl-L-proline butyl (tert.) And all protective groups of the resulting protected tetracosapeptide are dissociated in one or more steps in an acidic medium. (Machine-translation by Google Translate, not legally binding)