ES337456A1 - Antibiotics 16,511 R.P. and 18,051 R.P. and their preparation - Google Patents

Antibiotics 16,511 R.P. and 18,051 R.P. and their preparation

Info

Publication number
ES337456A1
ES337456A1 ES337456A ES337456A ES337456A1 ES 337456 A1 ES337456 A1 ES 337456A1 ES 337456 A ES337456 A ES 337456A ES 337456 A ES337456 A ES 337456A ES 337456 A1 ES337456 A1 ES 337456A1
Authority
ES
Spain
Prior art keywords
antibiotic
ethyl acetate
solvent
chromatography
nrrl
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES337456A
Other languages
Spanish (es)
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Rhone Poulenc SA
Original Assignee
Rhone Poulenc SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Rhone Poulenc SA filed Critical Rhone Poulenc SA
Publication of ES337456A1 publication Critical patent/ES337456A1/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07G—COMPOUNDS OF UNKNOWN CONSTITUTION
    • C07G11/00—Antibiotics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C12P1/06—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using actinomycetales
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/465—Streptomyces
    • C12R2001/55—Streptomyces hygroscopicus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/465—Streptomyces
    • C12R2001/56—Streptomyces lavendulae

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

The invention relates to an antibiotic 16,511 R.P., which is a yellow amorphous powder of m. pt. 114 C. and which is an acid forming salts with bases, e.g. alkali metal salts it is soluble in methanol and ethanol, methylene chloride, chloroform, ethyl acetate, dimethylformamide, dimethylsulphoxide and acetone, less so in benzene and diethyl ether and practically insoluble in water, cyclohexane and hexane it has the elementary analysis C= = 65À8%, H=7À8%, O=22À7%, N=3À55% its ultraviolet spectrum (Fig. 1) shows absorption max. at 232 nm. (E1% 1 cm =682) and 350 nm max. at 232 nm. (E1% 1cm = 682) and 350 nm (E1% 1 cm. = 375) and minimum at 260 nm (E1% 1 cm. =130) its infra-red spectrum is shown in Fig. 3 [[alpha]] D 22 = - 121 2 (C=1À0) it contains components A (Rf value=0À1), B (Rf=0À2) also known as 18,051 R.P., C (Rf= 0À35) and D (Rf=0À60). Rf value is determined by thin layer chromatography using a Kieselgel H support and ethyl acetate as solvent. Antibiotic 18,051 R.P. is a yellow amorphous powder of m. pt. 112 C. having the elementary analysis C=64À7%, H=8À2%, O=23À75%, N=3À35% U.V. as in Fig. 6 (not shown) I.R. as in Fig. 8 (not shown), and [[alpha]]22 D = - 116À5 5À5 (C=0À73). Antibiotic 16,511 R.P. is produced by aerobically cultivating Streptomyces hygroscopicus var. tepidalitus NRRL 3237, Streptomyces lavendulae NRRL 3277, Streptomyces lavendulae NRRL 3278, or a 16,511 R.P.- producing mutant thereof. 16,511 R.P. may be isolated from the fermentation broth by (a) solvent extraction of the whole or clarified broth, (b) adsorption or strongly anionic exchange resins from a clarified broth, followed by elution with aqueous alcohol containing an electrolyte, e.g. sodium chloride. Purification may be by (a) precipitation from solution by a non-solvent such as hexane, (b) chromatography on alumina, silica gel or florisil, (c) treatment with carbon. 18,051 R.P. is separated from 16,511 R.P. by (A) chromatography on Kieselgel buffered at pH 4À0 with M/15 phosphate buffer, the chromatogram being developed with ethyl acetate (B) selective extraction with a methylene chloride-cyclohexane M/15 phosphate buffer at pH 9À0 (80: 20: 100 by volume). The antibiotics are effective against Gram positive micro-organisms. Pharmaceutical compositions, e.g. injections, comprise at least one of 16,511 R.P. and 18,051 R.P. or a non-toxic salt thereof and a carrier.
ES337456A 1966-03-02 1967-03-02 Antibiotics 16,511 R.P. and 18,051 R.P. and their preparation Expired ES337456A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR51696A FR1516739A (en) 1966-03-02 1966-03-02 New antibiotics and their methods of preparation by culture of streptomyces hygroscopicus
FR91136 1967-01-13

Publications (1)

Publication Number Publication Date
ES337456A1 true ES337456A1 (en) 1968-03-01

Family

ID=26168950

Family Applications (1)

Application Number Title Priority Date Filing Date
ES337456A Expired ES337456A1 (en) 1966-03-02 1967-03-02 Antibiotics 16,511 R.P. and 18,051 R.P. and their preparation

Country Status (9)

Country Link
AT (1) AT270066B (en)
BE (1) BE694872A (en)
CH (1) CH472494A (en)
ES (1) ES337456A1 (en)
FR (2) FR1516739A (en)
GB (1) GB1152386A (en)
GR (1) GR32437B (en)
LU (1) LU53088A1 (en)
NL (1) NL6702707A (en)

Also Published As

Publication number Publication date
LU53088A1 (en) 1967-09-01
GR32437B (en) 1967-07-04
GB1152386A (en) 1969-05-14
FR1577210A (en) 1969-08-08
NL6702707A (en) 1967-09-04
FR1516739A (en) 1968-02-05
CH472494A (en) 1969-05-15
AT270066B (en) 1969-04-10
BE694872A (en) 1967-09-01

Similar Documents

Publication Publication Date Title
IE36434B1 (en) Gentamicin antibiotics
GB1058686A (en) The antibiotic rubradirin
ES373632A1 (en) Process for the preparation of daunorubicin
ES337456A1 (en) Antibiotics 16,511 R.P. and 18,051 R.P. and their preparation
GB1350271A (en) Antibiotic and a process for its preparation
ES252350A1 (en) The new organic compound tylosin, its hydrolysis product desmycosin and methods for preparing same
GB1303842A (en)
JPS5750994A (en) Novel antibiotic mf266 substance and its preparation
IE37892L (en) Antibiotic a.2315.
GB1226494A (en)
GB1299924A (en) The antibiotic sl3364
ELANDER et al. Synthesis of an antibiotic closely resembling fusidic acid by imperfect and perfect dermatophytes
IE41640L (en) Production of antibiotic substances
GB1212741A (en) A method for producing an antibiotic b-2847
GB1504994A (en) Naphthyridinomycin antibiotics and preparation thereof
GB1131079A (en) The antibiotic armentomycin
SE329371B (en)
US3817836A (en) Method of preparing macrolide antibiotics
GB1356723A (en) Antibiotic sf-1293 substance and its production and uses
GB1226665A (en)
ES341402A1 (en) Antibiotics of agricultural fungicides,polyoxins d,e,f,g and h
ES350779A1 (en) Antibiotic 19,402 r.p.
GB1314983A (en) Antibiotic substance and method of producing same
GB1101361A (en) The new antibiotic, shinomycin, and production thereof
Uchida et al. METABOLIC PRODUCTS OF MICROORGANISMS 137 RINAMYCIN, A NEW INHIBITOR OF RNA SYNTHESIS