ES338445A1 - Manufacture of Purified Amyloglucosidase Preparations. - Google Patents
Manufacture of Purified Amyloglucosidase Preparations.Info
- Publication number
- ES338445A1 ES338445A1 ES338445A ES338445A ES338445A1 ES 338445 A1 ES338445 A1 ES 338445A1 ES 338445 A ES338445 A ES 338445A ES 338445 A ES338445 A ES 338445A ES 338445 A1 ES338445 A1 ES 338445A1
- Authority
- ES
- Spain
- Prior art keywords
- manufacture
- solution
- transglucosidase
- amyloglucosidase
- aqueous solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 108010073178 Glucan 1,4-alpha-Glucosidase Proteins 0.000 title abstract 3
- 238000002360 preparation method Methods 0.000 title abstract 2
- 238000004519 manufacturing process Methods 0.000 title 1
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 abstract 3
- MUBZPKHOEPUJKR-UHFFFAOYSA-N Oxalic acid Chemical compound OC(=O)C(O)=O MUBZPKHOEPUJKR-UHFFFAOYSA-N 0.000 abstract 3
- 108090000790 Enzymes Proteins 0.000 abstract 2
- 102000004190 Enzymes Human genes 0.000 abstract 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 abstract 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 abstract 2
- 239000007864 aqueous solution Substances 0.000 abstract 2
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Substances OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 abstract 2
- 150000001875 compounds Chemical class 0.000 abstract 2
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 abstract 2
- 239000000243 solution Substances 0.000 abstract 2
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 abstract 1
- PXRKCOCTEMYUEG-UHFFFAOYSA-N 5-aminoisoindole-1,3-dione Chemical compound NC1=CC=C2C(=O)NC(=O)C2=C1 PXRKCOCTEMYUEG-UHFFFAOYSA-N 0.000 abstract 1
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 abstract 1
- 239000012141 concentrate Substances 0.000 abstract 1
- 238000000855 fermentation Methods 0.000 abstract 1
- 230000004151 fermentation Effects 0.000 abstract 1
- 239000000706 filtrate Substances 0.000 abstract 1
- 235000019253 formic acid Nutrition 0.000 abstract 1
- 238000011065 in-situ storage Methods 0.000 abstract 1
- 230000002779 inactivation Effects 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 238000001556 precipitation Methods 0.000 abstract 1
- 238000000746 purification Methods 0.000 abstract 1
- 230000001105 regulatory effect Effects 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
- 239000001117 sulphuric acid Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
A process for the purification of amyloglucosidase preparations comprises the inactivation in situ, without removal, of transglucosidase contained in an aqueous solution of the impure enzyme by incubating the solution at a pH below 2À5 without adding auxiliary substances other than a pH regulating compound which does not cause precipitation of the transglucosidase and which does not have surface-active properties. The compound added may be hydrochloric, sulphuric, phosphoric, citric, oxalic, acetic or formic acid. The aqueous solution treated may comprise the entire fermentation broth in which the amyloglucosidase has been formed, a culture filtrate, or a solution of an enzyme concentrate.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| NL6604012A NL6604012A (en) | 1966-03-26 | 1966-03-26 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES338445A1 true ES338445A1 (en) | 1968-04-01 |
Family
ID=19796102
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES338445A Expired ES338445A1 (en) | 1966-03-26 | 1967-03-25 | Manufacture of Purified Amyloglucosidase Preparations. |
Country Status (6)
| Country | Link |
|---|---|
| BE (1) | BE696051A (en) |
| DK (1) | DK113704B (en) |
| ES (1) | ES338445A1 (en) |
| FR (1) | FR1515697A (en) |
| GB (1) | GB1180802A (en) |
| NL (1) | NL6604012A (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4532213A (en) * | 1983-12-08 | 1985-07-30 | Miles Laboratories, Inc. | Recovery of acid fungal protease |
-
1966
- 1966-03-26 NL NL6604012A patent/NL6604012A/xx unknown
-
1967
- 1967-03-23 GB GB1384167A patent/GB1180802A/en not_active Expired
- 1967-03-24 BE BE696051D patent/BE696051A/xx unknown
- 1967-03-25 ES ES338445A patent/ES338445A1/en not_active Expired
- 1967-03-28 FR FR100522A patent/FR1515697A/en not_active Expired
- 1967-03-28 DK DK160767A patent/DK113704B/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| BE696051A (en) | 1967-09-25 |
| FR1515697A (en) | 1968-03-01 |
| DK113704B (en) | 1969-04-21 |
| NL6604012A (en) | 1967-09-27 |
| GB1180802A (en) | 1970-02-11 |
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