ES369475A1 - Procedure for the enrichment of polipeptides. (Machine-translation by Google Translate, not legally binding) - Google Patents
Procedure for the enrichment of polipeptides. (Machine-translation by Google Translate, not legally binding)Info
- Publication number
- ES369475A1 ES369475A1 ES369475A ES369475A ES369475A1 ES 369475 A1 ES369475 A1 ES 369475A1 ES 369475 A ES369475 A ES 369475A ES 369475 A ES369475 A ES 369475A ES 369475 A1 ES369475 A1 ES 369475A1
- Authority
- ES
- Spain
- Prior art keywords
- substance
- complex
- amino
- inhibitor
- addition product
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 238000000034 method Methods 0.000 title abstract 3
- 239000000126 substance Substances 0.000 abstract 6
- 239000003112 inhibitor Substances 0.000 abstract 4
- 229920001184 polypeptide Polymers 0.000 abstract 3
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 3
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 3
- ICSNLGPSRYBMBD-UHFFFAOYSA-N 2-aminopyridine Chemical compound NC1=CC=CC=N1 ICSNLGPSRYBMBD-UHFFFAOYSA-N 0.000 abstract 2
- 102000004190 Enzymes Human genes 0.000 abstract 2
- 108090000790 Enzymes Proteins 0.000 abstract 2
- 229940088598 enzyme Drugs 0.000 abstract 2
- 238000005406 washing Methods 0.000 abstract 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 2
- NAHSJIQXYWGMJN-UHFFFAOYSA-N 1-cyclohexylpropane-1,3-diamine Chemical compound NCCC(N)C1CCCCC1 NAHSJIQXYWGMJN-UHFFFAOYSA-N 0.000 abstract 1
- NGNBDVOYPDDBFK-UHFFFAOYSA-N 2-[2,4-di(pentan-2-yl)phenoxy]acetyl chloride Chemical group CCCC(C)C1=CC=C(OCC(Cl)=O)C(C(C)CCC)=C1 NGNBDVOYPDDBFK-UHFFFAOYSA-N 0.000 abstract 1
- NUKYPUAOHBNCPY-UHFFFAOYSA-N 4-aminopyridine Chemical compound NC1=CC=NC=C1 NUKYPUAOHBNCPY-UHFFFAOYSA-N 0.000 abstract 1
- QYPPJABKJHAVHS-UHFFFAOYSA-N Agmatine Natural products NCCCCNC(N)=N QYPPJABKJHAVHS-UHFFFAOYSA-N 0.000 abstract 1
- 239000004382 Amylase Substances 0.000 abstract 1
- 102000013142 Amylases Human genes 0.000 abstract 1
- 108010065511 Amylases Proteins 0.000 abstract 1
- 241000283690 Bos taurus Species 0.000 abstract 1
- 102000003670 Carboxypeptidase B Human genes 0.000 abstract 1
- 108090000087 Carboxypeptidase B Proteins 0.000 abstract 1
- 102000000496 Carboxypeptidases A Human genes 0.000 abstract 1
- 108010080937 Carboxypeptidases A Proteins 0.000 abstract 1
- 102000003914 Cholinesterases Human genes 0.000 abstract 1
- 108090000322 Cholinesterases Proteins 0.000 abstract 1
- 108090000746 Chymosin Proteins 0.000 abstract 1
- 108090000317 Chymotrypsin Proteins 0.000 abstract 1
- 241000196324 Embryophyta Species 0.000 abstract 1
- 108010088842 Fibrinolysin Proteins 0.000 abstract 1
- 108010003272 Hyaluronate lyase Proteins 0.000 abstract 1
- 102000001974 Hyaluronidases Human genes 0.000 abstract 1
- 108010067372 Pancreatic elastase Proteins 0.000 abstract 1
- 102000016387 Pancreatic elastase Human genes 0.000 abstract 1
- 108090000526 Papain Proteins 0.000 abstract 1
- 108010087702 Penicillinase Proteins 0.000 abstract 1
- 108090000284 Pepsin A Proteins 0.000 abstract 1
- 102000057297 Pepsin A Human genes 0.000 abstract 1
- 239000004365 Protease Substances 0.000 abstract 1
- 108010083644 Ribonucleases Proteins 0.000 abstract 1
- 102000006382 Ribonucleases Human genes 0.000 abstract 1
- 235000002595 Solanum tuberosum Nutrition 0.000 abstract 1
- 244000061456 Solanum tuberosum Species 0.000 abstract 1
- 108090000190 Thrombin Proteins 0.000 abstract 1
- 108090000631 Trypsin Proteins 0.000 abstract 1
- 102000004142 Trypsin Human genes 0.000 abstract 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 abstract 1
- QYPPJABKJHAVHS-UHFFFAOYSA-P agmatinium(2+) Chemical compound NC(=[NH2+])NCCCC[NH3+] QYPPJABKJHAVHS-UHFFFAOYSA-P 0.000 abstract 1
- 235000019418 amylase Nutrition 0.000 abstract 1
- 150000008064 anhydrides Chemical class 0.000 abstract 1
- 239000007853 buffer solution Substances 0.000 abstract 1
- 239000004202 carbamide Substances 0.000 abstract 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 abstract 1
- 229940048961 cholinesterase Drugs 0.000 abstract 1
- 229960002376 chymotrypsin Drugs 0.000 abstract 1
- 238000006073 displacement reaction Methods 0.000 abstract 1
- 238000010494 dissociation reaction Methods 0.000 abstract 1
- 230000005593 dissociations Effects 0.000 abstract 1
- 229960004979 fampridine Drugs 0.000 abstract 1
- 125000001153 fluoro group Chemical group F* 0.000 abstract 1
- 150000002357 guanidines Chemical class 0.000 abstract 1
- 229960002773 hyaluronidase Drugs 0.000 abstract 1
- 150000002500 ions Chemical class 0.000 abstract 1
- -1 kallicrein Proteins 0.000 abstract 1
- DILRJUIACXKSQE-UHFFFAOYSA-N n',n'-dimethylethane-1,2-diamine Chemical compound CN(C)CCN DILRJUIACXKSQE-UHFFFAOYSA-N 0.000 abstract 1
- 210000000496 pancreas Anatomy 0.000 abstract 1
- 229940055729 papain Drugs 0.000 abstract 1
- 235000019834 papain Nutrition 0.000 abstract 1
- 229950009506 penicillinase Drugs 0.000 abstract 1
- 229940111202 pepsin Drugs 0.000 abstract 1
- 229940012957 plasmin Drugs 0.000 abstract 1
- 235000012015 potatoes Nutrition 0.000 abstract 1
- 238000000746 purification Methods 0.000 abstract 1
- 239000011347 resin Substances 0.000 abstract 1
- 229920005989 resin Polymers 0.000 abstract 1
- 210000001625 seminal vesicle Anatomy 0.000 abstract 1
- 239000000243 solution Substances 0.000 abstract 1
- 229960004072 thrombin Drugs 0.000 abstract 1
- 239000012588 trypsin Substances 0.000 abstract 1
- 239000002753 trypsin inhibitor Substances 0.000 abstract 1
- 201000010653 vesiculitis Diseases 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6445—Kallikreins (3.4.21.34; 3.4.21.35)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
- C12N11/089—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/81—Protease inhibitors
- C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
- C07K14/811—Serine protease (E.C. 3.4.21) inhibitors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6427—Chymotrypsins (3.4.21.1; 3.4.21.2); Trypsin (3.4.21.4)
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Gastroenterology & Hepatology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
A process for the enrichment of a substance capable of forming a complex with a polypeptide which comprises linking a polypeptide by a covalent linkage to a carrier to form an addition product, introducing basic groups into the addition product, contacting the thus modified addition product in water with a solution of the substance to be enriched whereby a complex between the said substance and the addition product is formed, purifying the complex by washing, causing the complex to dissociate, and isolating the said substance. The substance to be purified may be an enzyme e.g. trypsin, chymotrypsin, kallicrein, plasmin, pepsin, rennin, ribonuclease, thrombin, amylase, papain, hyaluronidase, carboxypeptidase A or B, pancreatopeptidase E, penicillinase or cholinesterase, or an inhibitor such as kallicreintrypsin inhibitor ("Kuritz inhibitor") or the trypsin inhibitors obtained from pig and bovine pancreas, from seminal vesicles, from plant seeds or from potatoes. When the substance is an enzyme the polypeptide used in the process may be the corresponding inhibitor and vice versa. The carrier may be a resin containing anhydride or acid chloride groups or activated fluorine atoms, or carboxyl groups plus activated groups. The necessary basic groups may be introduced by treatment with N,N - dimethylethylene - diamine, agmatine, N - methyl- N1 - (3 - amino - propyl) - piperazine, 4- amino - pyridine, 2 - amino - pyridine, or 3- amino 1 - cyclohexyl - aminopropane. Purification of the complex may be by washing with water and buffer solutions, and dissociation may be by altering pH or ion concentration, by displacement, or by adding urea or guanidine salts.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19681768934 DE1768934A1 (en) | 1968-07-15 | 1968-07-15 | Process for the enrichment of polypeptides, preferably of enzymes and enzyme inhibitors |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES369475A1 true ES369475A1 (en) | 1971-06-01 |
Family
ID=5699923
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES369475A Expired ES369475A1 (en) | 1968-07-15 | 1969-07-14 | Procedure for the enrichment of polipeptides. (Machine-translation by Google Translate, not legally binding) |
Country Status (21)
| Country | Link |
|---|---|
| JP (1) | JPS5212242B1 (en) |
| AT (1) | AT287188B (en) |
| BE (1) | BE736069A (en) |
| CA (1) | CA923834A (en) |
| CH (1) | CH512522A (en) |
| CS (1) | CS150676B2 (en) |
| DE (1) | DE1768934A1 (en) |
| DK (1) | DK122433B (en) |
| EG (1) | EG10148A (en) |
| ES (1) | ES369475A1 (en) |
| FI (1) | FI47375C (en) |
| FR (1) | FR2014613A1 (en) |
| GB (1) | GB1228698A (en) |
| IE (1) | IE33418B1 (en) |
| IL (1) | IL32511A (en) |
| LU (1) | LU59055A1 (en) |
| NL (1) | NL6910881A (en) |
| NO (1) | NO124538B (en) |
| PL (1) | PL82620B1 (en) |
| RO (1) | RO61383A (en) |
| SE (1) | SE372276B (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4054646A (en) | 1973-07-30 | 1977-10-18 | General Electric | Method and apparatus for detection of antibodies and antigens |
| FR2802817B1 (en) * | 1999-12-23 | 2002-10-11 | Centre Nat Rech Scient | NOVEL GLYCOSIDASE INHIBITORS AND THEIR PHARMACOLOGICAL APPLICATIONS, PARTICULARLY FOR TREATING DIABETES |
| CA2655873C (en) | 2006-07-14 | 2017-10-17 | Wisconsin Alumni Research Foundation | Adsorptive membranes for trapping viruses |
| EP3196315B1 (en) | 2016-01-19 | 2020-02-05 | ORITEST spol. s r.o. | Spherical pellets, manufacturing process of such pellets, use, and a detection tube comprising such pellets |
| CN117099869B (en) * | 2023-06-27 | 2024-11-15 | 河南工业大学 | A preparation method of wheat protein peptide chelated calcium |
-
1968
- 1968-07-15 DE DE19681768934 patent/DE1768934A1/en active Pending
-
1969
- 1969-06-24 CH CH964469A patent/CH512522A/en not_active IP Right Cessation
- 1969-06-30 CA CA055716A patent/CA923834A/en not_active Expired
- 1969-06-30 IL IL6932511A patent/IL32511A/en unknown
- 1969-07-02 GB GB3335669A patent/GB1228698A/en not_active Expired
- 1969-07-04 IE IE918/69A patent/IE33418B1/en unknown
- 1969-07-04 AT AT643769A patent/AT287188B/en active
- 1969-07-05 RO RO6900060439A patent/RO61383A/en unknown
- 1969-07-08 LU LU59055A patent/LU59055A1/xx unknown
- 1969-07-14 CS CS4976A patent/CS150676B2/cs unknown
- 1969-07-14 JP JP44055134A patent/JPS5212242B1/ja active Pending
- 1969-07-14 PL PL1969134799A patent/PL82620B1/pl unknown
- 1969-07-14 SE SE6909961A patent/SE372276B/xx unknown
- 1969-07-14 DK DK380869AA patent/DK122433B/en unknown
- 1969-07-14 BE BE736069A patent/BE736069A/xx unknown
- 1969-07-14 NO NO2941/69A patent/NO124538B/no unknown
- 1969-07-14 ES ES369475A patent/ES369475A1/en not_active Expired
- 1969-07-15 FI FI692106A patent/FI47375C/en active
- 1969-07-15 EG EG381/69*A patent/EG10148A/en active
- 1969-07-15 NL NL6910881A patent/NL6910881A/xx unknown
- 1969-07-15 FR FR6924062A patent/FR2014613A1/fr not_active Withdrawn
Also Published As
| Publication number | Publication date |
|---|---|
| GB1228698A (en) | 1971-04-15 |
| EG10148A (en) | 1976-11-30 |
| DE1768934A1 (en) | 1972-03-16 |
| BE736069A (en) | 1970-01-14 |
| RO61383A (en) | 1977-10-15 |
| JPS5212242B1 (en) | 1977-04-06 |
| NL6910881A (en) | 1970-01-19 |
| FI47375B (en) | 1973-07-31 |
| LU59055A1 (en) | 1969-11-18 |
| IE33418L (en) | 1970-01-15 |
| DK122433B (en) | 1972-03-06 |
| AT287188B (en) | 1971-01-11 |
| CS150676B2 (en) | 1973-09-04 |
| CA923834A (en) | 1973-04-03 |
| CH512522A (en) | 1971-09-15 |
| SE372276B (en) | 1974-12-16 |
| NO124538B (en) | 1972-05-02 |
| IE33418B1 (en) | 1974-06-26 |
| IL32511A (en) | 1973-05-31 |
| FI47375C (en) | 1973-11-12 |
| PL82620B1 (en) | 1975-10-31 |
| FR2014613A1 (en) | 1970-04-17 |
| IL32511A0 (en) | 1969-11-12 |
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