ES427232A1 - A procedure to remove and insulate the enzymatic component from the venom of the serpents. (Machine-translation by Google Translate, not legally binding) - Google Patents
A procedure to remove and insulate the enzymatic component from the venom of the serpents. (Machine-translation by Google Translate, not legally binding)Info
- Publication number
- ES427232A1 ES427232A1 ES427232A ES427232A ES427232A1 ES 427232 A1 ES427232 A1 ES 427232A1 ES 427232 A ES427232 A ES 427232A ES 427232 A ES427232 A ES 427232A ES 427232 A1 ES427232 A1 ES 427232A1
- Authority
- ES
- Spain
- Prior art keywords
- venom
- elution
- eluate
- vitro
- translation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 238000000034 method Methods 0.000 title abstract 3
- 239000002435 venom Substances 0.000 title abstract 3
- 231100000611 venom Toxicity 0.000 title abstract 3
- 210000001048 venom Anatomy 0.000 title abstract 3
- 241000270295 Serpentes Species 0.000 title abstract 2
- 230000002255 enzymatic effect Effects 0.000 title abstract 2
- 238000010828 elution Methods 0.000 abstract 4
- 102000004190 Enzymes Human genes 0.000 abstract 3
- 108090000790 Enzymes Proteins 0.000 abstract 3
- 239000000701 coagulant Substances 0.000 abstract 3
- 238000000338 in vitro Methods 0.000 abstract 3
- 235000004252 protein component Nutrition 0.000 abstract 3
- 239000003146 anticoagulant agent Substances 0.000 abstract 2
- 229940127219 anticoagulant drug Drugs 0.000 abstract 2
- 230000000694 effects Effects 0.000 abstract 2
- 238000001727 in vivo Methods 0.000 abstract 2
- 238000010521 absorption reaction Methods 0.000 abstract 1
- 239000003125 aqueous solvent Substances 0.000 abstract 1
- 238000013375 chromatographic separation Methods 0.000 abstract 1
- 239000003112 inhibitor Substances 0.000 abstract 1
- 230000014508 negative regulation of coagulation Effects 0.000 abstract 1
- 231100000614 poison Toxicity 0.000 abstract 1
- 239000002574 poison Substances 0.000 abstract 1
- 239000003998 snake venom Substances 0.000 abstract 1
- 239000007787 solid Substances 0.000 abstract 1
- 239000000758 substrate Substances 0.000 abstract 1
Landscapes
- Extraction Or Liquid Replacement (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
- Peptides Or Proteins (AREA)
Abstract
A method for extracting and isolating snake venom from the enzymatic component having "in vitro" coagulant activity and "in vivo" anticoagulant activity from the other components having "in vitro" coagulant activity and from the protein components, whose process is characterized because the venom of the snakes, dissolved in a suitable medium for a chromatographic separation treatment, is passed through a column filled with a solid support for the selective absorption of said anticoagulant enzyme "in vivo", not soluble in aqueous solvents and combined to a substrate or inhibitor of said enzyme, a first elution medium is passed to ph 6-7 and a molarity less than or equal to 0.05% through said filling into which the poison has been introduced, is collected the eluate in successive portions until the elution of more than 95% of the protein components of the venom and the coagulant fraction "in vitro" has been obtained, different from the in the second eluate, a second elution medium is passed to a ph even higher than 9 and a molarity greater than or equal to 0.3m, and the eluate is collected in successive portions until elution of the anticoagulant enzyme is obtained. Live "absorbed in the column, along with less than 5% of the protein components. (Machine-translation by Google Translate, not legally binding)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IT4240973 | 1973-09-03 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES427232A1 true ES427232A1 (en) | 1976-09-01 |
Family
ID=11253965
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES427232A Expired ES427232A1 (en) | 1973-09-03 | 1974-06-12 | A procedure to remove and insulate the enzymatic component from the venom of the serpents. (Machine-translation by Google Translate, not legally binding) |
Country Status (1)
| Country | Link |
|---|---|
| ES (1) | ES427232A1 (en) |
-
1974
- 1974-06-12 ES ES427232A patent/ES427232A1/en not_active Expired
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