ES444543A1 - Immunological reagent and method of using same - Google Patents
Immunological reagent and method of using sameInfo
- Publication number
- ES444543A1 ES444543A1 ES444543A ES444543A ES444543A1 ES 444543 A1 ES444543 A1 ES 444543A1 ES 444543 A ES444543 A ES 444543A ES 444543 A ES444543 A ES 444543A ES 444543 A1 ES444543 A1 ES 444543A1
- Authority
- ES
- Spain
- Prior art keywords
- antigen
- antibody
- reaction
- mixture
- aqueous solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 239000003153 chemical reaction reagent Substances 0.000 title abstract 2
- 230000001900 immune effect Effects 0.000 title abstract 2
- 238000000034 method Methods 0.000 title abstract 2
- 239000000427 antigen Substances 0.000 abstract 2
- 239000007864 aqueous solution Substances 0.000 abstract 2
- 239000000203 mixture Substances 0.000 abstract 2
- 239000002202 Polyethylene glycol Substances 0.000 abstract 1
- 102000036639 antigens Human genes 0.000 abstract 1
- 108091007433 antigens Proteins 0.000 abstract 1
- 238000003556 assay Methods 0.000 abstract 1
- 239000002736 nonionic surfactant Substances 0.000 abstract 1
- 229920001223 polyethylene glycol Polymers 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5306—Improving reaction conditions, e.g. reduction of non-specific binding, promotion of specific binding
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicinal Preparation (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
A method of immunological determination involving a reaction of an antibody with an antigen to form an antibody-antigen complex, characterized in that the insolubilization of the antigen-antibody complex formed by said reaction is increased using a reagent in said assay method comprising an aqueous solution of a mixture of said polyethylene glycol and a nonionic surfactant, said aqueous solution containing, in use, about 3% to 6% by weight of the mixture, and having within said range a calculated value of ehl (hydrophilic equilibrium- lipophilic) comprised between about 0.7 to 1.7. (Machine-translation by Google Translate, not legally binding)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US54506675A | 1975-01-29 | 1975-01-29 | |
| US55590875A | 1975-03-06 | 1975-03-06 | |
| US05/615,024 US4148869A (en) | 1975-03-06 | 1975-09-19 | Immunological reagent and method of using same |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES444543A1 true ES444543A1 (en) | 1977-06-01 |
Family
ID=27415448
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES444543A Expired ES444543A1 (en) | 1975-01-29 | 1976-01-22 | Immunological reagent and method of using same |
Country Status (15)
| Country | Link |
|---|---|
| JP (1) | JPS604938B2 (en) |
| BE (1) | BE837675A (en) |
| BR (1) | BR7600322A (en) |
| CA (1) | CA1080619A (en) |
| CH (1) | CH613047A5 (en) |
| DE (2) | DE2602068C2 (en) |
| DK (1) | DK150809C (en) |
| ES (1) | ES444543A1 (en) |
| FR (1) | FR2299645A1 (en) |
| GB (1) | GB1540097A (en) |
| IL (1) | IL48804A (en) |
| IT (1) | IT1062433B (en) |
| NL (1) | NL183852C (en) |
| NO (1) | NO149864C (en) |
| SE (1) | SE441392B (en) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2901327B1 (en) * | 1979-01-15 | 1980-07-24 | Boehringer Mannheim Gmbh | Method for determining an opsonizing surface-binding alpha-glycoprotein |
| FI792576A7 (en) * | 1979-08-20 | 1981-01-01 | Orion Yhtymae Oy | Improved enzyme immunoassay method. |
| JPS5943362A (en) * | 1982-09-06 | 1984-03-10 | Kainosu:Kk | Immunological measuring reagent |
| AU580248B2 (en) * | 1982-10-13 | 1989-01-12 | Biowhittaker Technologies, Inc. | Fluorometric assay of allergic reactions and reagents therefor |
| GB8317855D0 (en) * | 1983-06-30 | 1983-08-03 | Iq Bio Ltd | Biochemical detection method |
| DE3327642A1 (en) * | 1983-07-30 | 1985-02-14 | Boehringer Mannheim Gmbh, 6800 Mannheim | METHOD FOR DETERMINING A PARTNER OF AN IMMUNE REACTION AND REAGENT FOR CARRYING OUT THIS METHOD |
| DE3532626A1 (en) * | 1985-09-12 | 1987-03-19 | Boehringer Mannheim Gmbh | METHOD FOR DETERMINING A PARTNER OF AN IMMUNE REACTION |
| JPS62159047A (en) * | 1985-12-31 | 1987-07-15 | Chemo Sero Therapeut Res Inst | Reagent for quantitative determination of plasma protein |
| US4810638A (en) * | 1986-07-24 | 1989-03-07 | Miles Inc. | Enzyme-labeled antibody reagent with polyalkyleneglycol linking group |
| JP2684069B2 (en) * | 1988-10-13 | 1997-12-03 | 昇一 首藤 | Method for measuring immunologically active substance and reagent therefor |
| CA2077906A1 (en) * | 1991-01-10 | 1992-07-11 | Kimihiko Matsuzawa | Method for measurement of tissue factor in high sensitivity and measurement kit therefor |
| CA2136757A1 (en) * | 1993-03-26 | 1994-10-13 | Robert C. Wohl | Method and assay involving improved specific binding reactivity of a polypeptide |
| DE602004021248D1 (en) * | 2003-04-03 | 2009-07-09 | Seed Co Ltd | CONTACT LENSES WITH THE ABILITY OF CONTINUOUS MEDICATION RELEASE AND PROTECTION SOLUTIONS THEREFOR |
| JP4915638B2 (en) * | 2005-08-26 | 2012-04-11 | パナソニック株式会社 | Electrodeless discharge lamp device and lighting fixture equipped with the electrodeless discharge lamp device |
| JP6242068B2 (en) * | 2013-04-10 | 2017-12-06 | 積水メディカル株式会社 | Latex immunoagglutination measurement reagent |
| TWI842764B (en) * | 2018-11-09 | 2024-05-21 | 日商積水醫療股份有限公司 | Methods for suppressing abnormal detections in immunoassays conducted with an automatic analyzer, detection methods, immunoassay reagents, and immunoassay reagent kits |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE1598151A1 (en) * | 1966-11-19 | 1970-05-27 | Kleine Dr Rer Nat Norbert | Agglutination enhancers |
| DE2361169C3 (en) * | 1973-12-07 | 1978-09-14 | Boehringer Mannheim Gmbh, 6800 Mannheim | Process for the activation of cholesterol oxidase freed from detergent traces |
| US3947250A (en) * | 1974-06-21 | 1976-03-30 | Baxter Laboratories, Inc. | Method of immunodiffusion |
-
1976
- 1976-01-07 IL IL48804A patent/IL48804A/en unknown
- 1976-01-12 CA CA243,325A patent/CA1080619A/en not_active Expired
- 1976-01-12 SE SE7600232A patent/SE441392B/en not_active IP Right Cessation
- 1976-01-15 FR FR7600986A patent/FR2299645A1/en active Granted
- 1976-01-16 GB GB1784/76A patent/GB1540097A/en not_active Expired
- 1976-01-16 DK DK016976A patent/DK150809C/en not_active IP Right Cessation
- 1976-01-16 IT IT19348/76A patent/IT1062433B/en active
- 1976-01-19 BE BE163598A patent/BE837675A/en not_active IP Right Cessation
- 1976-01-19 BR BR7600322A patent/BR7600322A/en unknown
- 1976-01-21 DE DE2602068A patent/DE2602068C2/en not_active Expired
- 1976-01-21 DE DE2661010A patent/DE2661010C2/en not_active Expired
- 1976-01-22 NO NO760203A patent/NO149864C/en unknown
- 1976-01-22 ES ES444543A patent/ES444543A1/en not_active Expired
- 1976-01-23 NL NLAANVRAGE7600697,A patent/NL183852C/en not_active IP Right Cessation
- 1976-01-23 JP JP51007067A patent/JPS604938B2/en not_active Expired
- 1976-01-27 CH CH102276A patent/CH613047A5/en not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| SE441392B (en) | 1985-09-30 |
| BE837675A (en) | 1976-07-19 |
| BR7600322A (en) | 1976-08-31 |
| DK150809B (en) | 1987-06-22 |
| NL7600697A (en) | 1976-08-02 |
| DK150809C (en) | 1988-05-02 |
| IL48804A0 (en) | 1976-03-31 |
| DE2661010C2 (en) | 1987-03-19 |
| NO149864C (en) | 1984-07-04 |
| JPS51101121A (en) | 1976-09-07 |
| CH613047A5 (en) | 1979-08-31 |
| CA1080619A (en) | 1980-07-01 |
| FR2299645A1 (en) | 1976-08-27 |
| DE2602068A1 (en) | 1976-08-05 |
| NO149864B (en) | 1984-03-26 |
| SE7600232L (en) | 1976-07-30 |
| NL183852C (en) | 1989-02-01 |
| DE2602068C2 (en) | 1986-09-11 |
| GB1540097A (en) | 1979-02-07 |
| NO760203L (en) | 1976-07-30 |
| IL48804A (en) | 1979-05-31 |
| FR2299645B1 (en) | 1980-11-28 |
| JPS604938B2 (en) | 1985-02-07 |
| IT1062433B (en) | 1984-10-10 |
| NL183852B (en) | 1988-09-01 |
| DK16976A (en) | 1976-07-30 |
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